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Maternal prenatal depression is associated with child sleep. We investigated whether maternal depression comorbid with anxiety worsens toddler's sleep problems in a prospective cohort study. A total of 1583 mother-infant pairs from the China-Anhui Birth Cohort study were examined. The participants completed the Center for Epidemiologic Studies Depression Scale (CES-D) and Self-Rating Anxiety Scale (SAS) at 30-34 weeks of gestation, and the Edinburgh Postnatal Depression Scale (EPDS) at 3-month postpartum. Toddler's sleep was assessed by the Brief Infant Sleep Questionnaire (BISQ) at 30 months old. Logistic regression models were used to investigate the associations between prenatal depression and anxiety and toddler's sleep, while adjusting for maternal gestational age, education, family income, alcohol use, premature birth, fetal growth restriction, mode of delivery, postnatal depression, and 3-month breastfeeding. In total, 9.0% of participants reported prenatal depression comorbid with anxiety symptoms, and the prevalence of depression, anxiety was 6.7% and 7.3%, respectively. Compared with mothers without depression and anxiety, maternal depression combined with anxiety were significantly associated with shorter total sleep duration (11.16 ± 1.06 h), longer settling time (29.25 ± 23.57 min), and higher risk of toddlers' sleep problems assessed by BISQ (OR = 2.09, 95% CI: 1.22-3.57) or parental report (OR = 1.84, 95% CI: 1.22-2.77). However, there was no significant association between maternal postnatal depression and toddler sleep behaviors. Maternal prenatal depression comorbid with anxiety significantly associated with poorer toddler's sleep. Strategies to regulate prenatal mood status should be considered during prenatal health care to improve children's sleep development., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asian countries. Its root and rhizome have long been used for the treatment of cardiovascular and hematological diseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous plant tissues is significant for characterizing the metabolic networks of Panax notoginseng , and this will also provide a highly informative approach to understand the complex molecular changes in the processing of Panax notoginseng ., Methods: Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize the spatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panax notoginseng ., Results: A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine, gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizome and root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during the steaming of Panax notoginseng root were also characterized in this study. And, a series of metabolites such as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng were successfully screened out and imaged., Conclusion: These spatially-resolved metabolite data not only enhance our understanding of the Panax notoginseng metabolic networks, but also provide direct evidence that a serious of metabolic alterations occurred during the steaming of Panax notoginseng ., Competing Interests: The authors declare no conflict of interest., (© 2021 The Korean Society of Ginseng. Publishing services by Elsevier B.V.)

The effects of the functional membrane covering on the gas emissions and bacterial community during dairy cow manure aerobic composting were investigated. A lab-scale aerobic composting experiment was conducted with the control group (CK), Gore group (Gore), and ZT group (ZT), namely, without and with two functional membranes. Covering the functional membrane retained heat and improved the seed germination index in Gore and ZT groups. Compared with the CK group, the Gore membrane decreased NH 3 and N 2 O emissions by 11.77% and 26.40%, respectively. The ZT membrane decreased N 2 O and CO 2 emissions by 68.44% and 1.56%, respectively. The Gore and ZT membranes decreased the global warming potential by 16.97% and 53.41%, respectively. Moreover, Covering the two functional membranes improved the Actinobacteria relative abundance and were conducive to the degradation of volatile solid. Altogether, membrane-covered aerobic composting is an important technology for the resource utilization of organic waste., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

In this study, the effects of covering the compost pile with a semi-permeable membrane in combination with intermittent aeration on the gas emissions during aerobic composting from the solid fraction of dairy manure at industrial scale were investigated. A large-scale composting experiment was carried out to compare a membrane-covered (CT) group with a control (CK) group. The results indicated that the CT group could maintain a suitable aerobic and positive micro-pressure environment. The carbon dioxide, methane, nitrous oxide, and ammonia emissions outside the membrane during the aeration interval were reduced by 64.23%, 70.07%, 54.87%, and 11.32%, respectively, compared with that inside the membrane. It was also determined that the methane and nitrous oxide emissions from the CT group were reduced by 99.89% and 60.48% relative to the CK group, confirming that the combined process represented a novel strategy for reducing gas emissions during dairy manure composting., (Copyright © 2021. Published by Elsevier Ltd.)

The effects of functional membrane covering (FMC) on nitrogen transformation and related functional genes during aerobic composting were investigated by performing a comparable experiment. The FMC increased the pile temperature, promoted compost maturity, and decreased nitrogen loss. The FMC reduced NH 3 and N 2 O emissions by 7.34% and 26.27%, respectively. The water film and the micro-positive pressure environment under the membrane effectively prevented NH 3 escaping. The FMC up-regulated the amoA gene copy number (promoting NH 3 /NH 4 + oxidation). The reduction of N 2 O emission by the FMC was mainly related to denitrifying genes (nirK, nirS, and nosZ). The FMC down-regulated the nirK and nirS gene copy numbers, but up-regulated the nosZ gene copy number. Pearson correlation analysis indicated that the functional membrane characteristics and differences between the composting pile environments caused by the FMC significantly affected the nitrogen forms and the related functional genes. The FMC strongly decreased nitrogen emissions and therefore conserved nitrogen., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Pseudogenes are accumulated in host-restricted Salmonella enterica serovars, while pseudogenization is primarily regarded as a process that purges unnecessary genes from the genome. Here we showed that the inactivation of sopA, which encodes an effector of Salmonella Pathogenicity Island 1, in human-restricted S. enterica serovar Typhi (S. Ty) and Paratyphi A (S. PA) is under positive selection and aimed to reduce bacterial cytotoxicity toward host macrophages. Moreover, we found that the expression of sopA in Salmonella Typhimurium (S. Tm), a broad-host-range serovar which causes systemic disease in mice, was negatively regulated during mice infection and survival in murine macrophages. The sopA repression in S. Tm is mediated by IsrM, a small RNA absent from the genome of S. Ty and S. PA. Due to the lack of IsrM, sopA expression was unregulated in S. Ty and S. PA, which might have facilitated the convergent inactivation of sopA in these two serovars. In conclusion, our findings demonstrate that sopA inactivation or intracellular repression is the target of positive selection during the systemic infection caused by S. enterica serovars., Competing Interests: Declaration of competing interest There is no conflict of interest., (Copyright © 2020 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)

Salmonella Typhimurium establishes systemic infection by replicating in host macrophages. Here we show that macrophages infected with S. Typhimurium exhibit upregulated glycolysis and decreased serine synthesis, leading to accumulation of glycolytic intermediates. The effects on serine synthesis are mediated by bacterial protein SopE2, a type III secretion system (T3SS) effector encoded in pathogenicity island SPI-1. The changes in host metabolism promote intracellular replication of S. Typhimurium via two mechanisms: decreased glucose levels lead to upregulated bacterial uptake of 2- and 3-phosphoglycerate and phosphoenolpyruvate (carbon sources), while increased pyruvate and lactate levels induce upregulation of another pathogenicity island, SPI-2, known to encode virulence factors. Pharmacological or genetic inhibition of host glycolysis, activation of host serine synthesis, or deletion of either the bacterial transport or signal sensor systems for those host glycolytic intermediates impairs S. Typhimurium replication or virulence.

Zn(II) can efficiently promote the catalytic performance of imidazolium salt ionic liquids (imi-ILs) for the chemical fixation of CO 2 into epoxides. To obtain sustainability, immobilized bifunctional catalysts containing both imi-ILs and Zn(II) were prepared using bimodal mesoporous silica (BMMs) as carrier, through grafting of Zn(OAc) 2 and 1-(trimethoxysilyl)propyl-3-methylimidazolium chloride (Si-imi) separately in the nanopores. The catalysts, named as BMMs-Zn&ILs, were identified as efficient catalysts for cycloaddition reaction of CO 2 into epoxides under solvent-free conditions. BMMs-Zn&ILs showed good catalytic activity, which increased with the increase of the molar ratio of Zn(II) to Si-imi. As a comparison, different catalytic systems including homogeneous imi-IL, BMMs-ILs and BMMs-Zn were studied to demonstrate different cooperation behaviors. Furthermore, the kinetics studies of homogeneous and heterogeneous bifunctional catalysts were employed to confirm the differences, as well as to support the proposed cooperative catalysis mechanism in the nanopores., (© 2020 Wiley-VCH GmbH.)

Background: Triple-negative breast cancer (TNBC) is a kind of malignant tumor with higher recurrence and metastasis rate. According to historical records, the dry rhizomes Cyperus rotundus L. could be ground into powder and mixed with ginger juice and wine for external application for breast cancer. We studied the effect of the ethanol extract of Cyperus rotundus L. (EECR) on TNBC cells and found its' apoptosis-inducing effect with a dose-relationship. But the function mechanism of EECR on TNBC is still mysterious. Hence, the present research aimed to detect its function mechanism at the small molecule level through ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) metabolomics., Methods: The CCK-8 assay and the Annexin V-FITC/PI assay were applied to test the effect of EECR on MDA-MB-231 cells and MDA-MB 468 cells at various concentrations of 0, 200, 400, and 600 μg/ml. UPLC-Q-TOF-MS/MS based metabolomics was used between the control group and the EECR treatment groups. Multivariate statistical analysis was used to visualize the apoptosis-inducing action of EECR and filtrate significantly changed metabolites., Results: The apoptosis-inducing action was confirmed and forty-nine significantly changed metabolites (VIP > 1, p < 0.05, and FC > 1.2 or FC < 0.8) were identified after the interference of EECR. The level of significant differential metabolites between control group, middle dose group, and high dose group were compared and found that which supported the apoptosis-inducing action with dose-dependence., Conclusion: By means of metabolism, we have detected the mechanism of EECR inducing apoptosis of TNBC cells at the level of small molecule metabolites and found that EECR impacted the energy metabolism of TNBC cells. In addition, we concluded that EECR induced apoptosis by breaking the balance between ATP-production and ATP-consumption: arresting the pathways of Carbohydrate metabolism such as Central carbon metabolism in cancer, aerobic glycolysis, and Amino sugar and nucleotide sugar metabolism, whereas accelerating the pathways of ATP-consumption including Amino Acids metabolism, Fatty acid metabolism, Riboflavin metabolism and Purine metabolism. Although further study is still needed, EECR has great potential in the clinical treatment of TNBC with fewer toxic and side effects.

Introduction: Dozens of Alzheimer's disease (AD)-associated loci have been identified in European-descent populations, but their effects have not been thoroughly investigated in the Hong Kong Chinese population., Methods: TaqMan array genotyping was performed for known AD-associated variants in a Hong Kong Chinese cohort. Regression analysis was conducted to study the associations of variants with AD-associated traits and biomarkers. Lasso regression was applied to establish a polygenic risk score (PRS) model for AD risk prediction., Results: SORL1 is associated with AD in the Hong Kong Chinese population. Meta-analysis corroborates the AD-protective effect of the SORL1 rs11218343 C allele. The PRS is developed and associated with AD risk, cognitive status, and AD-related endophenotypes. TREM2 H157Y might influence the amyloid beta 42/40 ratio and levels of immune-associated proteins in plasma., Discussion: SORL1 is associated with AD in the Hong Kong Chinese population. The PRS model can predict AD risk and cognitive status in this population., Competing Interests: All authors declare no conflicts of interests., (© 2020 The Authors. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring published by Wiley Periodicals, Inc. on behalf of the Alzheimer's Association.)

Profiling the spatial distributions and dynamic changes of metabolites in plant tissue is critical to elucidate the complex metabolic regulation during plant growth, development, and responses to abiotic or biotic stresses. In this study, we developed a high-coverage MALDI-MS imaging method to visualize the spatial locations of a wide spectrum of metabolites in Salvia miltiorrhiza Bge. 1,5-Diaminonaphthalene (DAN) and 1,1'-binaphthyl-2,2'-diamine (BNDM) were optimized as MALDI matrices in positive and negative ion modes respectively, due to their low background interference and high sensitivity. Moreover, a simple organic washing protocol using acetone was shown to significantly improve the sensitivity of MALDI-MSI. Using this method, we successfully imaged the spatial locations of amino acids, phenolic acids, fatty acids, oligosaccharides, cholines, polyamines, tanshinones, and phospholipids in Salvia miltiorrhiza Bge. In addition, the distributions of some metabolites in Salvia miltiorrhiza Bge root and stem were found to exhibit good spatial match with plant tissue structure. Thus, our method provides a spatially-resolved way to map the plant metabolic networks and to understand the physiological roles of several plant metabolites., Competing Interests: Declaration of Competing Interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019. Published by Elsevier B.V.)

With increasing air pollution, chronic bronchitis (CB) has become a major public health problem worldwide. Previous studies have shown beneficial effects of Bergenin (Ber) on chronic bronchitis. To facilitate understanding of the pathogenesis underlying CB as well as to elucidate the Ber therapeutic mechanism, it is crucial to confirm the rational biomarkers of CB and its treatment. This study aimed to investigate the preventive chronic bronchitis mechanism of Ber by applying a serum metabolomics strategy. In this study, 18 Sprague-Dawley rats were randomly divided into three groups,with six rats in each group. Rats from the CB and Ber groups were exposed to tobacco smoke for 1 hd -1 (1 h per day) for 28 days. Ber was administered orally to Ber rats 3 h after exposure every day, and the others were administered water. According to the morphometric analysis of the airway epithelium and the count of white blood cells in the bronchoalveolar lavage fluid, Ber suppressed the infiltration of inflammatory cells, inhibited the secretion of mucus, and reduced white blood cells in bronchoalveolar lavage fluid. The metabolic profiles of sera were analyzed by multivariate statistical analyses, including PCA, PLS-DA and OPLS-DA models, and revealed that the levels of thirteen metabolites were significantly changed and identified as potential biomarkers in the CB group and Ber group. The results suggested that the therapeutic mechanism of Ber may be related to the regulation of dysfunctions in glycerophospholipid, tryptophan, arginine and proline metabolism induced by CB, and changes in arachidonic acid metabolism., (Copyright © 2018 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)

It is very important to reduce methane production and emission during aerobic composting. In this study, the effects of covering with a semi-permeable membrane during pig manure and wheat straw composting were investigated. Two laboratory-scale composting reactors were used: the membrane covered treatment (treatment A) and the control treatment (treatment B). Composting in treatment A effectively improved the oxygen utilization rate and decreased methane emissions by 22.42% relative to the control treatment. Quantification of functional genes and Pearson rank correlations showed that the mcrA and mcrA/pmoA gene abundances were significantly positively correlated with temperature and negatively correlated with the interstitial oxygen concentration, and that the pmoA gene abundance was positively correlated with the carbon: nitrogen ratio and moisture content. Therefore, increasing the aeration rate and optimizing the carbon: nitrogen ratio and moisture content will decrease methane emissions. Together, the results demonstrate that coverage membrane could be a novel strategy for reducing methane emissions during composting., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Objective: To estimate the mediationl effects of psychological symptoms, coping styles and impulsiveness on relationship between childhood abuse and non-suicidal self-injuries( NSSI) among middle school students., Methods: A total of 9704 participants( boys( 4600), girls( 5104); urban( 4049), rural( 5655); junior( 5168), senior( 4536)) were retained from all epidemiological study, involving students from junior and senior middle schools in Zhengzhou, Guiyang by random cluster sampling. The Childhood Trauma Questionnaire( CTQ-SF), Non-Suicidal Self-Injury Questionnaire, Multidimensional Sub-health Questionnaire of Adolescents( MSQA), Trait Coping Style Questionnaire( TCSQ) and Barratt Impulsiveness Scale( BIS-11) were used to measure childhood abuse assessment, NSSI frequency, psychological symptoms, coping styles and impulsiveness scores. Chi-square test was used to compare the frequency of childhood abuse and NSSI among different groups. Pearson's correlation analysis was used in analyzing the relationship, Bootstrap method and PROCESS software were applied to examine the mediationl effects., Results: A total of 44. 6% of middle school students had childhood abuse experiences, girls were higher than boys( 45. 9% vs. 43. 1%), junior secondary students were higher than senior secondary students( 47. 3% vs. 41. 5%), the differences were statistically significant( χ~2= 7. 558 and 32. 433; P < 0. 01). However, there was no statistical significance in the residence area( P > 0. 05). 38. 5% of high school students had NSSI, of which girls were lower than boys( 37. 1% vs. 40. 1%), junior secondary students were higher than senior secondary students( 41. 4% vs. 35. 3%), the differences were statistically significant( χ~2= 9. 151 and 37. 955; P <0. 01). The middle of perceived family SES who the detection rate of childhood abuse and NSSI were lower than poor and high family SES, the differences were statistically significant( χ~2= 9. 916 and 25. 735; P < 0. 01). The psychological symptoms, coping style and impulsiveness scores were related with childhood abuse scores and NSSI frequency respectively( P < 0. 01). These mediating effects persisted after controlling for sex, grade, residence area and perceived family SES, the total mediationl effects was 52. 24%, specific mediationl effect of psychological symptoms, negative and positive coping styles, impulsiveness was 33. 41%, 14. 87%, 0. 49% and 3. 48%( P < 0. 01)., Conclusion: It suggests that psychological symptoms, coping styles, impulsiveness may play a partial mediating effect on the relationship between childhood abuse and NSSI among middle school students. The alleviating of psychological symptoms, negative coping style may contribute to the prevention and control of NSSI among middle school students who have experienced childhood abuse.

Background: The causal link between tobacco smoke exposure (TSE) and numerous severe respiratory system diseases (RSD), including chronic bronchitis, chronic obstructive pulmonary disease, and lung cancer, is well established. However, the pathogenesis of TSE-induced RSD remains incompletely understood. This research aims to detect the pathogenetic mechanisms and potential therapeutic targets of TSE-induced RSD., Methods: This study employed TSE model which rats were exposed to a concentration of 60% tobacco smoke in a toxicant exposure system for four weeks. Tandem mass tags (TMT) labeled quantitative proteomics combined with off-line high pH reversed-phase fractionation, and nano-liquid chromatography-mass spectrometry method (off-line high pH RPF-nano-LC-MS/MS) were adopted to detect differentially expressed proteins (DEPs) in the lung tissues of the TSE model rats and to compare them with those in control. The accuracy of the results was verified by western blot., Results: Compared with the control group, 33 proteins in the TSE model group's lung tissues showed significant differential expression. Analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways indicated that, several biological pathways, such as the steroid biosynthesis pathway, were involved and played significant roles in the pathogenesis of the experimental group's TSE., Conclusions: These findings make a crucial contribution to the search for a comprehensive understanding of TSE-induced RSD's pathogenesis, and furthermore provide guidance for the diagnosis and treatment of TSE-induced RSD., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Enhanced bursting activity of neurons in the lateral habenula (LHb) is essential in driving depression-like behaviours, but the cause of this increase has been unknown. Here, using a high-throughput quantitative proteomic screen, we show that an astroglial potassium channel (Kir4.1) is upregulated in the LHb in rat models of depression. Kir4.1 in the LHb shows a distinct pattern of expression on astrocytic membrane processes that wrap tightly around the neuronal soma. Electrophysiology and modelling data show that the level of Kir4.1 on astrocytes tightly regulates the degree of membrane hyperpolarization and the amount of bursting activity of LHb neurons. Astrocyte-specific gain and loss of Kir4.1 in the LHb bidirectionally regulates neuronal bursting and depression-like symptoms. Together, these results show that a glia-neuron interaction at the perisomatic space of LHb is involved in setting the neuronal firing mode in models of a major psychiatric disease. Kir4.1 in the LHb might have potential as a target for treating clinical depression.

The objective of the present study was to study the changes in compost particle and its relationship with other physicochemical process during aerobic composting employing 5%, 10%, 20% or 0% biochar. Changes of physicochemical and biological parameters and gases emissions indicated that appropriate biochar addition improved both degradation rate and final degree of the organic matter and simultaneously reduced CO 2 , CH 4 , N 2 O and NH 3 emissions. Beneficial properties like stability and high porosity of biochar could optimize composting environment, accelerate the process of composting and facilitate microbial growth during the thermophilic composting stage, with increases of 1.3×10 10 to 1.5×10 11 cfu/g. Analysis of microstructure characterization of the changes in compost particle indicated that biochar amended contributed to better degradation of compost particle with smaller sizes and a higher degree of looseness. Ultimately, 10% biochar addition optimized organic matter degradation, while reducing ammonia and greenhouse gas emissions and costs., (Copyright © 2017. Published by Elsevier Ltd.)

As a eukaryotic-like Ser/Thr protein kinase, Mycobacterium tuberculosis virulent effector protein kinase G (PknG) mediates mycobacterial survival by regulating bacterial cell metabolic processes and preventing phagosome-lysosome fusion in host macrophages. Targeting PknG is an effective strategy for development of anti-tuberculosis (TB) drugs. In the study, we found that sclerotiorin, derived from marine fungi from the South China Sea, exhibited moderately strong inhibitory effects on recombinant PknG, with an IC 50 value of 76.5 μM, and acted as a non-competitive inhibitor. The dissociation constant (K D ) of sclerotiorin determined by MST was 11.4 μM, demonstrating a moderate binding strength between them. Sclerotiorin could substantially impair the mycobacterial survival in infected macrophages while the macrophage viability remained unaffected, though it did not inhibit the mycobacterial growth in culture. When sclerotiorin was used in combination with rifampicin, intracellular mycobacterial growth decreased as sclerotiorin concentration increased. Docking analysis suggested a binding mechanism of inhibition with performing interactions with the P-loop and catalytic loop of PknG. In summary, we reported that sclerotiorin had moderately strong PknG inhibitory activity, but no cytotoxicity, and it could substantially decrease the mycobacterial growth inside macrophages, suggesting that sclerotiorin has potential to supplement antibiotic therapy for TB., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Proliferating cell nuclear antigen (PCNA) has been suggested as a potential diagnostic biomarker for early hepatocellular carcinoma (HCC). However, its prognostic significance in HCC remains unclear. In the present study, we investigated the expression and significance of PCNA in HCC and then analyzed the role of PCNA in clinical outcomes. Our findings show that the expression intensity of PCNA is much higher in HCC tissues than that in paracarcinoma tissues and associated with AFP, albumin, tumor number, clinical grade, vascular invasion, and tumor-node-metastasis (TNM) stage (all p < 0.000). Kaplan-Meier analysis indicated that high PCNA expression was associated with poor disease-free survival (DFS) (p < 0.000) and overall survival (OS) (p < 0.000) in a training cohort of 76 HCC patients. Multiple Cox regression analysis indicated PCNA acts as an independent predictor for DFS (p = 0.002) and OS (p = 0.004) in HCC patients. Along with pathological results, our systematic review also identified the expression of PCNA was closely associated with DFS and OS (both p < 0.000). In conclusion, this study suggested that PCNA is increased in HCC patients and is indeed a novel unfavorable biomarker for prognostic prediction for patients with this deadly disease.

Positron emission tomography (PET) using 18 F-fluorodeoxyglucose has been widely used for analyzing cellular metabolism. The present study aimed to evaluate the association between the diagnostic value of PET/computed tomography (CT) in patients with post-operative recurrent and metastatic colorectal cancer (CRC), and the different levels of carcinoembryonic antigen (CEA). A total of 105 suspected recurrent and metastatic CRC patients (67 males and 38 females; mean age, 48.5 years) were included in this retrospective study. All the patients underwent PET/CT examination. The differences in the PET/CT diagnostic values of CEA-positive and -negative patients with recurrent CRC following surgery were retrospectively analyzed and compared. Among the 105 CRC patients, 87 exhibited recurrence and metastasis, as confirmed by histopathological diagnosis or clinical follow-up data. By contrast, the PET/CT examination results revealed that 85 cases were true positives (a false positive foci was diagnosed in one of the patients), 18 were true negatives and 2 were false negatives. Correspondingly, the sensitivity and degree of accuracy were 97.7 and 97.1%, respectively. The detection rates of PET/CT for the recurrence and metastases were 85.3% in the CEA-positive group and 75.7% in the CEA-negative group. No significant differences were observed between the two groups. Overall, CEA levels do not help improve the detection rate of PET/CT in the recurrence and metastasis of CRC. PET/CT imaging has a high sensitivity and degree of accuracy in detecting recurrence and metastasis following CRC surgery. Therefore, this method is ideal for monitoring relapsed and metastatic foci of post-operative colon cancer cases.