Search

Induction of antitumor immunity using autologous tumor proteins is an attractive approach to cancer therapy. However, better methods and stimulants to present these autologous proteins back to the immune system are needed. Here, we identify streptavidin as a novel carrier protein and stimulant, and test the efficacy of both syngeneic (rat) and autologous vaccines (dogs) using streptavidin in combination with reduced soluble tumor proteins. Initial syngeneic vaccine studies in the 9L rat glioma model were used to optimize vaccine dose and selectivity. Cytokine and blood analysis was used to monitor the response. Rats receiving two vaccinations of syngeneic tumor vaccine demonstrated a statistically significant (P < 0.05) survival advantage compared with controls (adjuvant only). Notably, vaccination also led to remission rates of between 30% and 60% in the aggressive 9L glioma model. Antibodies to streptavidin were detected in the serum of vaccinated rats; however, antibody levels did not correlate with the response. The cytokine TNF-α was upregulated in vaccine-treated rats, whereas ICAM1 was downregulated. After engraftment, vaccinated rats maintained CD4(+), CD8(+) T cells, and total lymphocyte levels closer to normal baseline than those in the controls. Twenty-five dogs treated with autologous vaccine preparations using streptavidin as a stimulant showed no adverse reactions, irrespective of additional chemotherapy and other medications. In this study, we developed a novel method for producing syngeneic and autologous vaccines using streptavidin selectivity and immunogenicity. These vaccines show efficacy in the 9L glioma rat model. Safety was also demonstrated in canine patients presenting with cancer treated with autologous vaccine.

Purpose: Effects of resistance training and detraining on glucose and insulin responses to an oral glucose load, muscle fiber type, and muscular performance in the offspring of those with type 2 diabetes (familial insulin resistant (FIR)) were investigated., Methods: Six FIR participants and 10 controls (C) completed 9 wk of resistance training and 9 wk of detraining. Measures of strength and power, an oral glucose tolerance test, and a muscle biopsy to determine myosin heavy chain (MHC) fiber composition were taken at baseline (T1), after training (T2), and after detraining (T3)., Results: Three-repetition maximum increased (P ≤ 0.001) similarly in both groups in all strength measures, e.g., leg press (FIR T1, T2: 121 ± 34 kg, 186 ± 50 kg; C T1, T2: 137 ± 42 kg, 206 ± 64 kg, respectively (means ± SD)). Wingate peak power increased (FIR T1, T2: 505 ± 137 W, 523 ± 143 W; C T1, T2: 636 ± 211 W, 672 ± 223 W, respectively; P ≤ 0.005 (means ± SD)). Training reduced insulin area under the curve more (P = 0.050) in FIR (T1, T2: 1219 ± 734 pmol·L, 837 ± 284 pmol·L, respectively (means ± SD)) than that in C (T1, T2: 647 ± 268 pmol·L, 635 ± 258 pmol·L, respectively (means ± SD)). MHC distribution did not change with training. Strength (three-repetition maximum measures) decreased with detraining (P ≤ 0.001) although Wingate power did not. Detraining increased insulin area under the curve (P = 0.018) in FIR (T2, T3: 837 ± 285 pmol·L, 1040 ± 194 pmol·L, respectively (means ± SD)) but not in C (T2, T3: 635 ± 258 pmol·L, 625 ± 213 pmol·L, respectively (means ± SD)). MHC IIX fibers increased with detraining (P = 0.026)., Conclusion: FIR appears to have exaggerated responses to resistance training and detraining, with a greater reduction in insulin release with glucose ingestion after training and increase when training ceases. Resistance training has a significant effect on insulin responses and may reduce future risk of type 2 diabetes mellitus among FIR.

It is perceived that long haul travel, comprising of rapid movement across several time zones is detrimental to performance in elite athletes. However, available data is equivocal on the impact of long haul travel on maximal explosive movements. The aim of this study was to quantify the impact of long haul travel on lower body muscle performance. Five elite Australian skeleton athletes (1 M, 4 F) undertook long haul flight from Australia to Canada (LH(travel)), while seven national team Canadian skeleton athletes (1 M, 6 F) acted as controls (NO(travel)). Lower body power assessments were performed once per day between 09:30 and 11:00 h local time for 11 days. Lower body power tests comprised of box drop jumps, squat jump (SJ) and countermovement jumps (CMJ). The LH(travel) significantly decreased peak and mean SJ velocity but not CMJ velocity in the days following long haul flight. CMJ height but not SJ height decreased significantly in the LH(travel) group. The peak velocity, mean velocity and jump power eccentric utilisation ratio for the LH(travel) group all significantly increased 48 h after long haul flight. Anecdotally athletes perceived themselves as 'jet-lagged' and this corresponded with disturbances observed in 'one-off' daily jumping ability between 09:30 and 11:00 h after eastward long haul travel from Australia to North America when compared to non-travel and baseline controls.

The aims of this study were to talent transfer, rapidly develop, and qualify an Australian female athlete in the skeleton event at the 2006 Torino Winter Olympic Games and quantify the volume of skeleton-specific training and competition that would enable this to be achieved. Initially, 26 athletes were recruited through a talent identification programme based on their 30-m sprint time. After attending a selection camp, 10 athletes were invited to undertake an intensified skeleton training programme. Four of these athletes were then selected to compete for Australia on the World Cup circuit. All completed runs and simulated push starts were documented over a 14-month period. The athlete who eventually represented Australia at the Torino Winter Olympic Games did so following approximately 300 start simulations and about 220 training/competition runs over a period of 14 months. Using a deliberate programming model, these findings provide a guide to the minimum exposure required for a novice skeleton athlete to reach Olympic representative standard following intensified sport-specific training. The findings of this study are discussed in the context of the deliberate practice theory and offer the term "deliberate programming" as an alternative way of incorporating all aspects of expert development.

This study characterizes key elements of the start in elite female World Cup skeleton athletes. The top 20 female competitors in three World Cup races were videotaped within a calibrated space to allow the following components of the start to be quantified: (1) acceleration (velocity at 15-m mark, time to 15-m mark), (2) capacity (time to load, total number of steps to load), and (3) load (velocity at 45-m mark). A correlation analysis was used to establish the relationship between the variables of interest and overall start time (15- to 65-m mark). Velocity at the 15-m mark accounted for 86% of the variance in overall start time at St. Moritz and 85% at Sigulda. A stepwise regression analysis revealed that approximately 89% of the variation in start time could be explained by velocity at the 15-m mark, time to load, and velocity at the 45-m mark. Of the variables analysed in this study, rapid acceleration to attain a high velocity at the 15-m mark was the most important component of a fast overall start time. The importance of the time to load and velocity at the 45-m mark vary according to the different track characteristics.

The winter sliding sport known as skeleton requires athletes to produce a maximal sprint followed by high speed sliding down a bobsled track. Athletes are required to complete the course twice in 1 hour and total time for the 2 runs determines overall ranking. The purpose of this investigation was to examine the effect of whole-body vibration (WBV) on lower body power to explore the utility of WBV as an ergogenic aid for skeleton competition. Elite skeleton athletes (1 male and 6 females) completed an unloaded squat jump (SQJ) immediately followed by 2 countermovement jumps (CMJs) and a maximal 30-m sprint before and after WBV or no vibration (CON) using a crossover design. The second 30-m sprint was slower following both CON (1.4% decrement; p = 0.05) and WBV (0.7% decrement; p = 0.03). Mean vertical velocity was maintained following WBV in the SQJ but decreased following CON (p = 0.03). There was a trend for athletes to commence the SQJ from a higher starting stance post-WBV compared to CON (p = 0.08). WBV decreased total vertical distance traveled compared to CON in the SQJ (p = 0.006). WBV had little effect on peak velocity, jump height, dip, and peak acceleration or any CMJ parameters. When sprint athletes' warm up and perform maximal jumps and a 30-m sprint with 15-20 minutes of recovery before repeating the sequence, the second series of performances tend to be compromised. However, when WBV is used before the second series of efforts, some aspects of maximal jumping and sprinting appear to be influenced in a beneficial manner. Further research is required to explore whether WBV can improve the second sprint for athletes in actual competition and/or what sort of WBV protocol is optimal for these populations.

Objective: To quantify the impact of eastward long haul travel on diurnal variations in cortisol, psychological sensations and daily measurements of physical performance., Methods: Five elite Australian skeleton athletes undertook a long haul eastward flight from Australia to Canada (LH(travel)), while seven elite Canadian skeleton athletes did not travel (NO(travel)). Salivary cortisol was measured on awakening, 60 min and 120 min after awakening. Psychological sensations were measured with a questionnaire, and maximal 30 m sprints were performed once a day between 09:30 and 11:00 h local time., Results: Compared with baseline, average (SD) resting salivary cortisol decreased by 67% immediately after long haul travel (23.43 (5.71) nMol/l) (mean+/-90% confidence interval) in the LH(travel) group (p = 0.03), while no changes were found in the NO(travel) group (p = 0.74). There were no significant differences in 30 m sprint time between baseline and post-flight tests in the LH(travel) group (p>0.05). The LH(travel) group perceived themselves as "jet lagged" for up to 2 days after the flight (p = 0.01 for both midday lunch and evening dinner)., Conclusions: Despite a distinct phase change in salivary cortisol rhythmicity and the athletes perceiving themselves as "jet lagged", minimal disturbances in "one-off" maximal sprinting ability between 09:30 and 11:00 h local time were seen in a group of elite skeleton athletes after long haul eastward travel from Australia to Canada.

Acceleration is an important factor for success in team-sport athletes. The purpose of this investigation was to compare the reliability of 10-m sprint times when using different starting techniques. Junior male rugby players (n = 15) were tested for speed over 10 m on 2 different testing sessions. Three trials of 3 different starting techniques (standing, foot, and thumb starts) were assessed. Despite large differences in the time taken to perform 10-m sprints from different starts, there was minimal difference in the typical error (approximately 0.02 seconds, or <1%) between the 3 different starts. There was a small, 0.02 +/- 0.02 second, decrease (p = 0.05) in sprint time between sessions for the foot start. For all starting techniques, the magnitude of error (typical error) was greater than the smallest worthwhile change (<0.01 second), indicating that acceleration over 10 m measured by photocells only has a marginal chance of reliably detecting a change of sufficient magnitude to be worthwhile in practical terms. However, by accounting for the smallest worthwhile change and typical error, it is possible to establish the probability an individual has had a worthwhile change in sprint performance. Coaching and sports-science practitioners can use a variety of sprint-start techniques shown to have small typical errors (<1%); however, the results from the different starting technique are not interchangeable.