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1,439 results on '"Wang, Yongfeng"'

356. Identification of potential therapeutic targets in urothelial bladder carcinoma of Chinese population by targeted next-generation sequencing

Author

Kaiyan Zhang, Tao Wang, Jinchun Xing, Anran Sun, Bin Chen, Xuegang Wang, Zhengsheng Liu, Wang Yongfeng, Wen Xiao, Zhun Wu, Zhiqiang Shao, Rongtuan Luo, Peide Bai, Wei Li, and Bo Duan

Subjects
0301 basic medicine, Oncology, Adult, Cancer Research, medicine.medical_specialty, Gene mutation, DNA sequencing, 03 medical and health sciences, 0302 clinical medicine, Chinese han population, Internal medicine, Carcinoma, Medicine, Humans, Urothelial carcinoma, Aged, Pharmacology, Aged, 80 and over, Chinese population, Bladder cancer, business.industry, High-Throughput Nucleotide Sequencing, Middle Aged, medicine.disease, 030104 developmental biology, Urinary Bladder Neoplasms, 030220 oncology & carcinogenesis, Mutation, Molecular Medicine, Risk of death, business, Research Paper
Abstract

Patients with urothelial carcinoma (UC) of the bladder have a high risk of death in China. However, a lack of comprehensive molecular profiling in Chinese Han population hinders the development of targeted therapies for bladder cancer. In our present study, we collected fresh bladder tumors from low-grade (T1, N0, M0, G1) non-muscle invasive bladder cancer (NMIBC) patients (n = 16) and high-grade (T2-4, N0, M0, Gx) muscle-invasive bladder cancer (MIBC) patients (n = 16) with their paired normal bladder tissues, and subjected the total genomic DNAs to targeted next-generation sequencing (NGS) for 94 cancer-associated genes. NGS results showed that 30.9% of detected genes (29/94) was mutated in 32 urothelial carcinoma bladder tissues. Furthermore, our results and ICGC database showed that FGFR3, KMT2D, TP53, KDM6A, and ARID1A were the most frequently mutated genes in UC patients. Of note, NMIBC and MIBC displayed distinguishable genomic alterations. FGFR3, KMT2D, AKT1, ARID1A, and STAG2 were the most frequently mutated genes in NMIBC patients, whereas mutations of TP53, CREBBP, FGFR3, KDM6A, KMT2D, and ARID1A were frequently detected in MIBC. Intriguingly, gene ontology and clustering analysis revealed that these frequently mutated genes were highly enriched in signaling pathways responsible for cancer development. Taken together, the mutation frequency of genes associated with UC development in NMIBC and MIBC was screened out in Chinese Han population and elucidation of the related mechanisms provides theoretical basis and technical support for the development of early diagnosis and therapeutic strategies in UC.

Published
2020

358. Effect of Temperature on RCS Measure and Calibration of Standard Metal Ball up to 800°C

Author

Lyu Ming, Wang Yongfeng, Zhao Yilun, Xiaofeng Yuan, Jinchun Li, Wang Yan, and Qi Kainan

Subjects
Imagination, Electromagnetic field, Electromagnetics, Materials science, Chemical substance, business.industry, media_common.quotation_subject, Ranging, Temperature measurement, Amplitude, Optics, Ball (bearing), Physics::Accelerator Physics, business, media_common
Abstract

Metal balls are often used as standard bodies in the target RCS measure based on precise theoretical calculations, and the accuracy of RCS measure data is directly related to the experiment measure. This paper mainly studies the calibration of metal balls at high temperatures in target RCS measure. Firstly, the high temperature field of targets is analyzed, and it is found that the changes of amplitude and phase distributions of the electromagnetic field surrounding the heated target. Then the echo of the standard metal ball at the ambient temperature is obtained through experiments, and the data is used as the calibration. Several temperature points of the target ranging from 30° to 800°C are selected for RCS measure to obtain the standard body RCS data, and get the experiment error less than 0.5 dB based on the temperature dependence of RCS data of 200 mm diameter metal ball. Therefore, RCS data are not sensitive to the temperature below 800°C, so the high temperature performance measure of the stealth targets can be calibrated at ambient temperature, which can obviously improve the efficiency at the high temperature.

Published
2019

359. Multislice computed tomography performance in differential diagnosis of high-density thymic cyst and thymoma in lesions less than 3 cm

Author

Pu Zhitao, Hu Bibo, Wang Yongfeng, Wu YiJiao, Jin Yinhua, Jin Zhonggao, Zheng Jianjun, Wang Jun, and Li Diansheng

Subjects
Pulmonary and Respiratory Medicine, Thymoma, Receiver operating characteristic, business.industry, High density, General Medicine, Multislice computed tomography, Thymic cyst, medicine.disease, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Oncology, 030220 oncology & carcinogenesis, Carcinoma, medicine, Differential diagnosis, Nuclear medicine, business, Pathological
Abstract

BACKGROUND This study was conducted to evaluate the performance of multislice computed tomography (MSCT) to diagnose high-density thymic cysts and thymomas in lesions measuring < 3 cm. METHODS The records of 42 patients admitted to Ningbo No. 2 Hospital with an anterior mediastinal mass (diameter < 3 cm) suspected of carcinoma originating from the thymus were retrospectively analyzed. All patients underwent surgery and pathological examination. Twenty-eight were diagnosed with thymic cysts and 14 with thymoma. The features of thymic cysts and thymomas revealed on MSCT were compared. RESULTS Mediastinal masses with a triangular or teardrop shape and a straight mediastina-lung border were more likely to be diagnosed as thymic cysts (P < 0.05), while those with a bulging nodule-lung border were more likely thymomas (P < 0.05). Using the CT value as a reference for differential diagnosis, the diagnostic sensitivity, specificity, and area under the receiver operating characteristic curve for CT values of non-contrast CT images, enhanced CT values, and ΔCT values were 0.65 (0.47-0.84), 0.95(0.86-1.00), and 0.96 (0.89-1.00), respectively. CONCLUSION MSCT of high-density thymus gland nodules (maximum diameter ≤ 3 cm) can reveal specific features that could be helpful for differential diagnosis.

Published
2018

360. Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus

Author

Shunqiang Xie, Jinchun Xing, Xuegang Wang, Rui Wan, Wen Xiao, Tao Wang, Bin Chen, Wang Yongfeng, Kaiyan Zhang, Zhun Wu, Lianguo Shi, Peide Bai, Rongtuan Luo, Jiang Deng, and Wei Li

Subjects
0301 basic medicine, Cancer Research, medicine.medical_specialty, Angiomyolipoma, Urology, Antineoplastic Agents, Hematocrit, Metastasis, 03 medical and health sciences, 0302 clinical medicine, White blood cell, Biopsy, Tuberous Sclerosis Complex 2 Protein, medicine, Humans, Everolimus, Pharmacology, Red Cell, medicine.diagnostic_test, Cell growth, business.industry, Epithelioid Cells, Middle Aged, medicine.disease, Prognosis, Kidney Neoplasms, Bedside-to-Bench Report, 030104 developmental biology, medicine.anatomical_structure, Oncology, Response Evaluation Criteria in Solid Tumors, 030220 oncology & carcinogenesis, Mutation, Molecular Medicine, Female, business, medicine.drug
Abstract

People who suffers renal angiomyolipoma (AML) has a low quality of life. It is widely known that genetic factors including TSC2 mutation contribute to certain populations of renal AML-bearing patients. In this study, we are the first to identify novel TSC2 mutations in one Chinese renal epithelioid AML patient: c.2652C>A; c.2688G>A based on sequencing result from biopsy tissue. These two somatic mutations cause a translational stop of TSC2, which leads to mTORC1 activation. Given the fact that activation of mTORC1 ensures cell growth and survival, we applied its inhibitor, FDA-approved everolimus, to this woman. After months of treatment with everolimus, Computer-Tomography (CT) scan results showed that everolimus successfully reduced tumor growth and distal metastasis and achieved partial response (PR) to everolimu according to Response Evaluation Criteria in Solid Tumors (RECIST version 1.1). Further Blood Routine Examination results showed the concentration of red cell mass, hemoglobin, white blood cell (WBC), platelets and hematocrit (HCT) significantly returned to normal levels indicating patients with these two TSC2 mutations could be effectively treated by everolimus.

Published
2019

373. Studying soil viral ecology under an ecosystem services framework.

Author

Liang, Xiaolong, Wang, Yongfeng, Xie, Ninghui, Wang, Shuo, Zhang, Ying, and Radosevich, Mark

Subjects
*VIRAL ecology, *SOIL ecology, *MICROBIAL ecology, *ECOSYSTEM services, *BIOGEOCHEMICAL cycles, *TECHNOLOGICAL innovations
Abstract

Viruses, the most abundant life form on Earth, are key regulators of microbial cellular and community properties. They can drive substantial microbial mortality (killing large proportions of microbial cells in the environment each day) and have profound effects on biogeochemical cycles. Recent studies suggest that viruses are a central member of soil trophic networks and can function across a wide range of spatial scales, thereby having critical roles in terrestrial ecosystem services. However, soil viral ecology research is still in its infancy stages, and it remains challenging to systematically assess the role of viruses in soil food webs and ecosystem function. With an urgent need to increase soil ecosystem productivity and sustainability, interpreting the ecological function of soil viruses presents major opportunities for better understanding critical soil ecosystem processes. In this work, we propose new perspectives of studying the function of soil viruses under an ecosystem services framework, which revolves around fundamentals of virus-host interactions, methodology for studying soil viral ecology, virus-driven shifts in microbial communities, impacts of soil viruses on ecosystem functionality and livelihood, and viral responses to a changing climate. • Fundamental study of soil virosphere has been overlooked. • Experimental evidence of soil viruses affecting ecological processes is urgently needed. • Systematically assessing the function of soil viruses requires technical innovations. • The ecological role of soil virosphere under global change is an emerging knowledge gap. • Incorporating soil viruses into soil ecosystem services framework is proposed. [ABSTRACT FROM AUTHOR]

Published
2024
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374. Efficacy and safety of Pien Tze Huang capsules in patients with herpes zoster: A multicenter, randomized, double-blinded, and placebo-controlled trial.

Author

Wu, Wenfeng, Yang, Dingquan, Sui, Daoshun, Zhu, Minghua, Luo, Guangpu, Yang, Zhonghui, Wang, Yongfeng, Luo, Hong, Ling, Li, Zhang, Zexin, Wu, Yanmei, Feng, Guoming, and Li, Hongyi

Abstract

Herpes zoster (HZ) is a common medical condition accompanied by several distressing symptoms, including acute pain. Pien Tze Huang (PZH) is a well-known traditional Chinese medicine (TCM) with numerous pharmacological effects, including antiviral properties, neuroprotection, and immunity regulation. To investigate the efficacy and safety of PZH capsules in patients with HZ. A multicenter, double-blinded, randomized, and placebo-controlled trial from 8 hospitals in 5 cities of China. Eligible participants were randomly assigned to the PZH capsule and placebo group at a 1:1 ratio. Treatment was conducted for 14 days with a window period of no more than 2 days. For the first 7 days, participants received antiviral drugs combined with PZH capsules (0.6 g/time, 3 times a day) or placebos. For the remaining 7 days, they were only treated with PZH capsules (0.6 g/time, 3 times a day) or placebos. We included 222 patients in the full analysis set (FAS), and 187 patients in the per protocol set (PPS). The change of numeric rating scale pain scores from baseline to the seventh day (±1 day) after treatment in the PZH capsule group was statistically superior to the placebo group (FAS: 2.33 vs. 1.71, 97.5% CI : 0.03 ∼ 1.19; PPS: 2.29 vs. 1.51, 97.5% CI : 0.18 ∼ 1.38). In the PPS, there was a significant difference in the time (days) of pain relief between the placebo group and the PZH capsule group (Mean (SD): 5.71 (3.76) vs. 4.69 (3.57), p = 0.046). On the seventh day (±1 day) after treatment, the level of CD8+ cells in the PZH capsule group were higher than those of the placebo group (FAS: Mean (SD): 24.08 (6.81) vs. 21.93 (8.19), p = 0.007; PPS: Mean (SD): 24.26 (6.93) vs. 22.15 (8.51), p = 0.012). The level of cytotoxic lymphocyte cells found similar results on the seventh day (±1 day) (FAS: Mean (SD): 12.17 (4.65) vs. 10.55 (4.15), p = 0.018; PPS: Mean (SD): 12.25 (4.65) vs. 10.11 (3.93), p = 0.002). No serious adverse events were noted and PZH capsules were well tolerated. PZH capsules confer therapeutic effects on HZ with the TCM symptom of stagnated heat of liver channel by substantially reducing the pain intensity, shortening the time of pain relief as well as regulating the immune function. On the basis of the efficacy and safety profiles, PZH capsules may be a promising complementary therapy for the treatment of HZ. [ABSTRACT FROM AUTHOR]

Published
2024
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375. Identification of WRKY transcription factors responding to abiotic stresses in Brassica napus L.

Author

Chen, Hao, Wang, Yongfeng, Liu, Jiong, Zhao, Tian, Yang, Cuiling, Ding, Qunying, Zhang, Yanfeng, Mu, Jianxin, and Wang, DaoJie

Subjects
RAPESEED, TRANSCRIPTION factors, ABIOTIC stress, CELLULAR signal transduction, GENE mapping, ABSCISIC acid
Abstract

Main conclusion: A total of 278 BnWRKYs were identified and analyzed. Ectopic expression of BnWRKY149 and BnWRKY217 suggests that they function in the ABA signaling pathway. WRKY transcription factors play an important role in plant development, however, their function in Brassica napus L. abiotic stress response is still unclear. In this study, a total of 278 BnWRKY transcription factors were identified from the B. napus genome data, and they were subsequently distributed in three main groups. The protein motifs and classification of BnWRKY transcription factors were analyzed, and the locations of their corresponding encoding genes were mapped on the chromosomes of B. napus. Transcriptome analysis of rapeseed seedlings exposed to drought, salt, heat, cold and abscisic acid treatment revealed that 99 BnWRKYs responded to at least one of these stresses. The expression profiles of 12 BnWRKYs were examined with qPCR and the result coincided with RNA-seq analysis. Two genes of interest, BnWRKY149 and BnWRKY217 (homologs of AtWRKY40), were overexpressed in Arabidopsis, and the corresponding proteins were located to the nucleus. Transgene plants of BnWRKY149 and BnWRKY217 were less sensitive to ABA than Arabidopsis Col-0 plants, suggesting they might play important roles in the responses of rapeseed to abiotic stress. [ABSTRACT FROM AUTHOR]

Published
2022
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393. Identification of potential therapeutic targets in urothelial bladder carcinoma of Chinese population by targeted next-generation sequencing

Author

Wang, Tao, primary, Liu, Zhengsheng, additional, Wang, Xuegang, additional, Bai, Peide, additional, Sun, Anran, additional, Shao, Zhiqiang, additional, Luo, Rongtuan, additional, Wu, Zhun, additional, Zhang, Kaiyan, additional, Li, Wei, additional, Xiao, Wen, additional, Duan, Bo, additional, Wang, Yongfeng, additional, Chen, Bin, additional, and Xing, Jinchun, additional

Published
2020
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394. Identification of Ala2Thr mutation in insulin gene from a Chinese MODY10 family

Author

Zhang, Juan, primary, Liu, Yanjun, additional, Li, Ming, additional, Ge, Xiaoxu, additional, Wang, Ying, additional, Huang, Xin, additional, Yang, Di, additional, Zhang, Rong, additional, Chen, Yating, additional, Lu, Ming, additional, Yin, Jun, additional, Song, Mingqiang, additional, Wang, Yongfeng, additional, Wang, Yanzhong, additional, Wang, Feng, additional, Jiang, Meisheng, additional, and Liu, Limei, additional

Published
2020
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