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A biotechnology for personalized ex vivo gene therapy based on molecular genomic balancing of hematopoietic stem cell (HSC) chromatin with nucleosome monomers of human genomic DNA (hDNA nmr ) has been developed and implemented in the clinic to change (to "correct") mutant chromosome loci genomes of dominant HSC clones that form mono- and oligoclonal hematopoiesis during aging and major (oncological, cardiovascular, neurodegenerative and autoimmune) fatal immune-mediated diseases of civilization. A fundamentally new biotechnological approach has been applied to the delivery of genetic material into eukaryotic stem and progenitor cells by establishing an artificial "recombinogenic situation" in them to induce homologous recombination (equivalent replacement) of mutant DNA regions with healthy hDNA nmr . In experimental preclinical trials, the effectiveness of genomic balancing technology has been proven to reduce the risk of sudden death in old animals and to increase the lifespan of outbred mice by 30% and Wistar rats by 57%. The improvement in their quality of life, compared with the control, is explained by an increase in the telomeric regions of the HSCs and HPCs chromosomes by 1.5-2 times. The potential of the technology to slow down the hereditary neurodegenerative diseases on the model of amyotrophic lateral sclerosis is shown. The effectiveness of this technology in clinical practice is presented on the example of a terminal patient with stage 4 neuroendocrine cancer. This technology used in the treatment of a number of oncological, neurodegenerative, autoimmune and hereditary diseases with clonal hematopoiesis is able to arrest the progression of the disease, prevent its recurrence, prolong the active life of a person, increase the average life expectancy and prevent sudden death., (Copyright © 2023. Published by Elsevier Inc.)

The comparative study of the protein markers of the membrane surface of the autologous hematopoietic stem cells (СD34+ СD45+) was performed to detect the patterns of the proteomic profiles, if any. The study included five groups. The subpopulations of the hematopoietic stem cells were studied in 569 samples of the mobilized peripheral blood mononuclear cells from adult cancer cases (group 1), 557 samples from children cancer cases (group 2), 66 samples from amyotrophic lateral sclerosis cases (group 3), 5 samples from other neurodegenerative diseases cases (supplementary group 4) and 61 samples isolated from healthy donors (control group 5). The protein markers of the membrane surface of the autologous hematopoietic stem cells were mapped and profiled with flow cytometry. The specific patterns of the proteomic profiles characteristic for cancer and neurodegenerative disease and different from those of healthy donors were identified. The authors suppose that as far as the HSC is the parent cell of the immune system cells, the modification of its proteomic profile speaks of specific immune insufficiency that not only accompanies the disease but presents the key cause for its onset and precedes its clinical manifestation. Thus, the evaluation of the protein markers of the membrane surface of hematopoietic stem cells can be used for ultra early diagnosis of these diseases., Competing Interests: Conflict of interests No conflict of interest., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Introduction: In 2014 and 2015 Professor of neurology Andrey Bryukhovetskiy published a novel theory of the information-commutation organization of the human brain in Russia, China and the USA. The theory posits the hypothesis that the higher nervous activity (cognitive, intellectual, mnestic) of the humans and their mind are material and have microwave electromagnetic nature. The theory perceives the human mind as a result of dynamic extracortical information-commutation relations of the super-positions of the electromagnetic waves of ultra high frequency emitted by different areas of the human brain in the inter-membrane cerebrospinal fluid space of the human head at a certain period of time. The inter-membrane cerebrospinal fluid space of the human head (the space between the dura, arachnoid and pia mater filled with the cerebrospinal fluid) of about 10mm size, has all morphological attributes to realize the holography. It is a universal natural bioprocessor for processing, analysis and synthesis of the input data and their record or reproduction on the pia as on the biological holographic membrane. The theory suggested that the processes of the mind can be recorded and digitalized with the last generation contemporary microwave receptors of the UHF band., Goal: The goal is to experimentally test the theory of the information-commutation organization of the human brain, particularly, the postulate that the human mind has material, and, namely, electromagnetic nature represented by the microwave bioelectric activity; it must be detected, recorded and statistically processed, i.e. its existence must be confirmed., Methods: On their own initiative, the team of mathematicians, radioengineers and neurologists performed the non-invasive research of the electromagnetic radiation of human brain in the broad frequency range varying from 850MHz to 26.5GHz with the last generation specialized measuring equipment with high sensitivity and recording speed, specialized measuring antennas and low noise amplifying equipment in the anechoic chamber of the 1st class of protection according to the Russian system of certification GOST R 50414-92., Results: The previously unknown microwave electromagnetic radiation of the EHF/UHF range (from 1.5GHz to 4.5GHz) with signal strength of -130dBm .. -100dBm (1e -15 .. 1e -13 W) are discovered. The detected electromagnetic waves have zonal variations in the different areas of the human head and are absent in other areas of the human body. The method of recording of the microwave electromagnetic activity of the human brain is patented in the Russian Federation. The microwave electromagnetic activity of the brain is billion-fold different from the bioelectric activity recorded by the encephalography., Conclusion: Discovery of the phenomenon of the microwave radiation of the human brain provides evidence to the idea that thinking and mind are material. This phenomenon has the potential to become a new informational channel of the diagnostics of the functional and pathological state of the higher nervous activity of the human brain. It can provide the basis for the development of the equipment for real-time analysis of the microwave bioelectric activity of the brain in norm and pathology, for objective early diagnostics of the functional and emotional conditions as well as of the psychiatric disorders at the preclinical stage, for the biocontrol of the human brain and the artificial simulators of the human brain. It also can provide the foundation for new systems of the artificial intellect, brain-computer interface and systems of the closed-loop biomanagement of the damaged brain., (© 2020 Elsevier B.V. All rights reserved.)

Introduction: Amyotrophic lateral sclerosis (ALS) is also known as motor neuron disease (MND) or Lou Gehrig's disease. It is a fatal neurodegenerative disease the cause of which is not clear. The effective therapy is absent. ALS is diagnosed through clinical examination and neurophysiologic tests. Clinically, the symptoms manifest when about 80% of motor neurons are dead., Materials and Methods: The hematopoietic stem cells are isolated through administration of the granulocyte colony-stimulating factor from three groups: group 1 of 62 ALS cases, group 2 of 54 ALS-free healthy donors and group 3 of 6 ALS-free ALS-family members. The expression of HLA-DR, CD38, CD117, CD13, CD33, CD56, Thy-1, CD45, СD10, CD71 was assessed in 86 samples of HSCs in ALS group, 61 samples of HSCs in healthy group and 6 samples from ALS-free ALS-family members by the multiparameter flow cytometry., Results: The obtained immunophenotypic profiles of HSCs membrane antigens of the ALS group significantly differ from the ALS-free group, while the immunophenotypic profiles of HSCs membrane antigens of the ALS-family members group are close to the ALS group., Discussion: We suppose that the ALS onset as the disease of HSCs and manifests in the genome and proteome of the HSCs. Such immunophenotypic profiling might permit identification of ALS-specific immune insufficiency and become a tool for early diagnostics of the ALS before clinical manifestation of the disease. New options of the updated therapy of ALS might be developed or corrected considering this new evidence., Conclusion: Further research with larger samples and deeper examination is required., (© 2020 Elsevier B.V. All rights reserved.)

Immunophenotype of mobilized stem blood cells (CD34+) was studied in 29 patients with late post-traumatic spinal lesions. The CD34+ cells demonstrated different levels of expression of CD45, CD38, monomorphic determinants HLA-DR and gp130 epitopes. Most patients presented with a CD34+ cell fraction with no or low expression of common leukocytic antigen CD45. Only 2 patients had greater than 15 percent of HLA-DR-CD38- cells in the CD34+ fraction. A common transducer molecule of interleukin-6 family cytokines gp130 was expressed on stem (CD34+) cells in all the cases, 26 percent of the patients had an activated gp130 phenotype, i.e. a combination of C7+ and A1- epitopes.