201. Hawthorn fruit extract ameliorates H 2 O 2 -induced oxidative damage in neuronal PC12 cells and prolongs the lifespan of Caenorhabditis elegans via the IIS signaling pathway.
- Author
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Wang X, Li X, Li L, Yang X, Wang J, Liu X, Chen J, Liu S, Zhang N, Li J, and Wang H
- Subjects
- Animals, Antioxidants metabolism, Antioxidants pharmacology, Caenorhabditis elegans genetics, Catalase metabolism, Fruit metabolism, Glutathione Peroxidase metabolism, Hydrogen Peroxide metabolism, Hydrogen Peroxide toxicity, Insulin-Like Growth Factor I metabolism, Lactate Dehydrogenases metabolism, Longevity, Malondialdehyde metabolism, Molecular Docking Simulation, Oxidative Stress, PC12 Cells, Paraquat, Phosphatidylinositol 3-Kinase metabolism, Phosphatidylinositol 3-Kinases genetics, Phosphatidylinositol 3-Kinases metabolism, Plant Extracts metabolism, Plant Extracts pharmacology, Proto-Oncogene Proteins c-akt metabolism, Rats, Reactive Oxygen Species metabolism, Signal Transduction, Superoxide Dismutase metabolism, Ultraviolet Rays, Crataegus, Insulins metabolism
- Abstract
Hawthorn ( Crataegus pinnatifida ) fruit has a long history of use as traditional Chinese medicine and is shown to have many health benefits including antioxidant and anti-aging. In this study, the anti-aging mechanism of hawthorn fruit extract (HFE) is predicted by network pharmacology and further verified in H
2 O2 -induced PC12 cells and Caenorhabditis elegans . Network pharmacology predicted that the antiaging mechanism of HFE is mainly involved in phosphoinositide 3-kinase (PI3K)/AKT and the insulin/insulin-like growth factor-1 (IIS) signaling pathway. HFE significantly improved cell viability, increased superoxide dismutase, catalase, and glutathione peroxidase activity, decreased lactate dehydrogenase release, the level of reactive oxygen species (ROS), and malondialdehyde content in H2 O2 -induced PC12 cells ( p < 0.05). HFE significantly increased the mean lifespan of C. elegans by 28.43% (100 μg mL-1 ) and enhanced the stress resistance to H2 O2 , paraquat, juglone, ultraviolet radiation, and heat shock. HFE also suppressed the accumulation of aging pigments, improved the body bending ability, increased antioxidant enzyme activities, and reduced the contents of ROS and malondialdehyde. In addition, relevant gene expression, lifespan experiments with mutant strains, and molecular docking studies supported the results that HFE might extend lifespan through the IIS signal pathway.- Published
- 2022
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