201. Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation.
- Author
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Cui YR, Hong C, Zhou YL, Li Y, Gao XM, and Zhang XX
- Subjects
- Animals, Antibodies, Monoclonal chemistry, Antibodies, Monoclonal immunology, CD3 Complex immunology, CD3 Complex metabolism, Magnetite Nanoparticles ultrastructure, Mice, Microscopy, Electron, Transmission, Reproducibility of Results, Spectroscopy, Fourier Transform Infrared, Spleen cytology, T-Lymphocytes cytology, T-Lymphocytes metabolism, X-Ray Diffraction, Cell Separation methods, Ferric Compounds chemistry, Gold chemistry, Magnetite Nanoparticles chemistry
- Abstract
Orientedly bioconjugated core/shell Fe(3)O(4)@Au magnetic nanoparticles were synthesized for cell separation. The Fe(3)O(4)@Au magnetic nanoparticles were synthesized by reducing HAuCl(4) on the surfaces of Fe(3)O(4) nanoparticles, which were further characterized in detail by TEM, XRD and UV-vis spectra. Anti-CD3 monoclonal antibody was orientedly bioconjugated to the surface of Fe(3)O(4)@Au nanoparticles through affinity binding between the Fc portion of the antibody and protein A that covalently immobilized on the nanoparticles. The oriented immobilization method was performed to compare its efficiency for cell separation with the non-oriented one, in which the antibody was directly immobilized onto the carboxylated nanoparticle surface. Results showed that the orientedly bioconjugated Fe(3)O(4)@Au MNPs successfully pulled down CD3(+) T cells from the whole splenocytes with high efficiency of up to 98.4%, showing a more effective cell-capture nanostructure than that obtained by non-oriented strategy. This developed strategy for the synthesis and oriented bioconjugation of Fe(3)O(4)@Au MNPs provides an efficient tool for cell separation, and may be further applied to various fields of bioanalytical chemistry for diagnosis, affinity extraction and biosensor., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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