1. Characteristics of in vitro rumen fermentation and microbial protein synthesis of different protein source leaves.
- Author
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Ilahude, Meygi, Sudarman, Asep, and Jayanegara, Anuraga
- Subjects
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RUMEN fermentation , *MICROBIOLOGICAL synthesis , *PROTEIN synthesis , *FERMENTATION , *BLOCK designs , *FATTY acids - Abstract
The purpose of this study was to assess the quality of three protein source leaves, i.e., cassava (M. esculanta), leucaena (L. leucocephala), and indigofera (I. zollingeriana) in terms of in vitro rumen fermentation and microbial protein synthesis. Rumen fluid as a source of microbial inoculum was collected five times from each of two sheeps (a total of 10 sheeps were used). The experimental design used was a randomized block design (RBD) with four treatments and five replicates (groups). The treatments used were the leaves of cassava (P1, CP 23%), leucaena (P2, CP 25.86%), indigofera (P3, CP 27.76%) and concentrate (P4 as control, CP 16.42%), and the grouping was based on the time of rumen fluid taken. The results showed that the provision of leaves of different protein sources had a significant effect (P<0.05) on ammonia (NH3) and volatile fatty acid (VFA) with the highest value shown in treatment P3 and the values decreased in P1, P2 and P4 treatments. However, the concentration of acetate showed the higher value (P<0.05) in the P2 treatment and the lower in the P4, while the higher value (P<0.05) for propionat was in the P4 treatment and the lower in the P3. Methane emission showed the higher value (P<0.05) in treatment P2 and the lower in P4. There was no significant effect on rumen pH and microbial protein synthesis. It could be concluded that protein source leaves provide better rumen fermentation profiles than that of concentrate diet, and Indigofera zollingeriana leaf was the best among the three leaves. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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