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This study aimed to develop an antioxidative compound extraction method for Kratom (Mitragyna speciosa) leaves using cellulase and pectinase, in combination and alone. The enzymatic hydrolysis-assisted process (EA) successfully enhanced antioxidant extraction from kratom leaves. The single enzymatic hydrolysis-assisted process (SEA) with 6% cellulase (C6) provided the highest extraction yield. The mixed enzymatic hydrolysis-assisted process (MEA) was then developed using C6, mixed with different concentrations of pectinase. Based on the high extraction yield, the MEA using 6% cellulase and 2% pectinase (C6+P2) was selected for further studies in comparison with that from relevant SEA and the control (without EA). Different extraction processes altered the characteristics and antioxidative activities of resulting extracts. The antioxidative activity of C6+P2 was dramatically enhanced in the gastrointestinal tract model system. Fourier transform infrared (FT-IR) spectrometer results confirmed significant content of phenolic compounds, proteins, and polysaccharides in C6+P2. From in vitro cytotoxicity study, C6+P2 showed an IC 50 value of 22.86 and 4.76 µg/mL in RAW264.7 and Caco-2 cells, respectively. The bioactive compounds from C6+P2 should be identified in further studies to facilitate their application in the food and pharmaceutical industries. [Display omitted] • 6% Cellulase mixed with 2% pectinase (C6+P2) gave the highest extraction yield. • C6+P2 showed drastically increase in antioxidative activity after digestion. • FTIR spectra reconfirmed the complex form of antioxidative compounds. • Cytotoxicity of C6+P2 was evaluated as IC 50 on macrophage and epithelial cells. [ABSTRACT FROM AUTHOR]

Summary: Whey and sunflower meals are primary by‐products of dairy cheese and vegetable oil production, respectively. Due to their widespread abundance, their valorisation could enable the generation of various value‐added food ingredients or products. In this study, an effort was made to utilise enzymatic pectinolysis along with other pre‐treatments to generate mixtures from whey and sunflower meal (SFM). The increasing presence of SFM increased the critical denaturation temperature of the mixtures and induced less protein structural changes. In addition, the presence of complex carbohydrates in SFM enhanced water and oil holding capacities. In general, pectinase and ultrasonication led to enhanced foaming of the mixtures, although the foams were relatively short‐lived. SFM inclusion limited foaming and emulsification capacities of the blends. In most cases, increasing the ratio of SFM to protein resulted in lower total reducing sugars (TRS), suggesting a more limited enzymatic activity. On the other hand, heating temperature or ultrasonication improved the extent of pectinolysis. Pre‐treatments and pectinolysis improved the functional properties of SFM and whey protein concentrate blends and are promising as an alternative protein source in new product applications. [ABSTRACT FROM AUTHOR]

This study discussed the impact of pectinase treatment on the properties of starch/PBAT composite, using two types of plant fibers such as coconut coir and bamboo fibers. The fibers underwent pretreatment with pectinase assisted by ultrasonics, and the composite was subsequently produced through hot pressing. The research focused on evaluating the thermal properties, mechanical properties, moisture absorption properties, and microstructure of the resulting composites. The findings revealed that the inclusion of pectinase‐treated plant fibers significantly enhanced the composite's strength. In particular, the bending strength of the composite with 15 wt% bamboo fibers exhibited the highest improvement, registering a 65% increase compared to the composite without added fibers. Additionally, the linear expansion coefficient of the plant fiber composite treated with pectinase was notably lower than that without added fibers. Among these, the linear expansion coefficient of the 15 wt% coir fiber composite decreased by 73.4% in the temperature range of 40–80°C compared to the composite without added fibers. The observed effects were attributed to the pectinase treatment, which proved effective in disrupting the network structure of cell walls, eliminating pectin components from fibers, and facilitating enhanced interaction between the fillers and the polymer matrix. Highlights: The inclusion of fibers significantly enhanced the composite's strength.10 wt% of bamboo fibers had a greater impact on the composite's strength than coir fiber.The linear expansion coefficient of the plant fiber composite decreased.The composite with 5 wt% bamboo fiber demonstrated excellent moisture resistance.The pectinase treatment enhanced interaction between fillers and matrix. [ABSTRACT FROM AUTHOR]

Fungal endophytes inhabit a similar ecological niche to that occupied by many phytopathogens, with several pathogens isolated from healthy tissues in their latent phase. This study aimed to evaluate the pathogenicity, the colonisation ability, and the enzyme activity of 37 endophytic fungal isolates recovered from apparently healthy apple shoot and leaf tissues. The pathogenicity of the isolates was assessed on 'Royal Gala' and 'Braeburn' fruit and detached 'Royal Gala' shoots. For the non-pathogenic isolates, their ability to endophytically colonise detached 'Royal Gala' shoots was evaluated. Enzyme activity assays were undertaken to determine whether the pathogenicity of the endophytes was related to the production of the extracellular enzymes, amylase, cellulase, pectinase, protease, and xylanase. Of the 37 isolates studied, eight isolates, representing the genera Colletotrichum , Diaporthe , Fusarium, and Penicillium, were shown to be pathogenic on both apple shoots and fruit. Two isolates identified as Trichoderma atroviride , were pathogenic only on shoots, and three isolates, representing the genus Diaporthe , were pathogenic only on fruit. Of the remaining 24 isolates, 22 (Biscogniauxia (n = 8), Chaetomium (n = 4), Trichoderma (n = 3), Epicoccum (n = 2), Neosetophoma (n = 2), Xylaria (n = 1), Daldinia (n = 1), and Paraphaeosphaeria (n = 1)) were recovered from the inoculated apple shoots but two failed to colonise the shoot tissues. Of the isolates tested, 20 produced amylase, 15 cellulase, 25 pectinase, 26 protease, and 13 xylanase. There was no correlation between the range and type of enzymes produced by the isolates and their pathogenicity or ability to endophytically colonise the shoot tissue. The study showed that approximately one-third (13/37) of the isolates recovered from the apparently healthy apple shoot tissues were observed as latent pathogens. The isolates that did not cause disease symptoms may have the ability to reduce colonisation of apple tissues by pathogens including Neonectria ditissima associated with European canker of apple. • Latent plant pathogens isolated from healthy plant tissue identified as endophytes. • Pathogenicity and enzyme production of 37 fungal apple endophyte isolates assessed. • Thirteen isolates produced disease symptoms on apple fruit and/or detached shoots. • Twenty-two isolates endophytically colonised apple shoots, with no symptoms. • Enzyme production did not differentiate between pathogenic and endophytic isolates. [ABSTRACT FROM AUTHOR]

In this work, we assessed genipin activation of chitosan particles and pectinase immobilization parameters, and after applying these biocatalysts for fruit juice clarification. Support activation pH and enzyme load, along with immobilization pH, were evaluated. Pectinex Ultrapulp was used for enzyme immobilization. The immobilized enzyme maintained around 60% initial activity after 5 h at 60ºC, compared to free enzyme's rapid decline in <1 h. Orange juice clarification showed no statistical difference between free and immobilized enzyme, while grape juice clarification exhibited a significant increase with immobilized enzyme compared to free enzyme. In pectin (0.1%) hydrolysis batch test, the immobilized preparation retained 40% relative initial activity over 20 batches. For fruit juice clarification stability, an improved washing approach, to remove adsorbed juice particles in the biocatalysts, helped maintain 40% initial capabilities after 10 cycles. This study demonstrates the potential of genipin-activated chitosan particles for immobilizing pectinase and enhancing fruit juice clarification. [Display omitted] • Biocatalyst presented 21.9% of immobilization yield and activity of 851.9 U.g−1 using 100 mg.g−1 of protein. • Immobilized pectinase kept 60% of initial activity after 5 h at 60 °C, outperforming free enzyme. • Grape juice clarification was improved with immobilized pectinase compared to the free enzyme. • Juice particles tended to be adsorbed by the support particles after reutilization process. • Improved washing approach sustains 40% initial capabilities after 10 cycles. [ABSTRACT FROM AUTHOR]

BACKGROUND: Cherry tomatoes are nutritious and favored by consumers. Processing them into dried cherry tomatoes can prolong their storage life and improve their flavor. The pretreatment of tomato pericarp is crucial for the subsequent processing. However, the traditional physical and chemical treatments of tomato pericarp generally cause nutrient loss and environmental pollution. RESULTS: In this study, a novel enzymatic method for cherry tomatoes was performed using mixed enzymes containing cutinase, cellulase and pectinase. Results showed that the pericarp permeability of cherry tomatoes was effectively improved due to enzymatic treatment. Changes in the microscopic structure and composition of the cuticle were revealed. After treatment with different concentrations of enzymes, cherry tomatoes exhibited higher pericarp permeability and sensory quality to varying degrees. The lycopene content and total polyphenol content significantly increased 2.4- and 1.45-fold, respectively. In addition, the satisfactory effect of the six-time reuse of enzymes on cherry tomatoes could still reach the same level as the initial effect, which effectively reduced the cost of production. CONCLUSIONS: This study revealed for the first time that a mixed enzymatic treatment consisting of cutinase, pectinase and cellulase could effectively degrade the cuticle, enhance the pericarp permeability and improve the quality of cherry tomatoes, with the advantages of being mildly controllable and environmentally friendly, providing a new strategy for the processing of dried cherry tomatoes. [ABSTRACT FROM AUTHOR]

Enzymatic hydrolysis using pectinase is critical for producing high-yield and quality sea buckthorn juice. This study determined the optimal temperature, time, and enzyme dosage combinations to guide manufacturers. A temperature of 60 °C, hydrolysis time of 3 h, and 0.3% enzyme dosage gave 64.1% juice yield—25% higher than without enzymes. Furthermore, monitoring physicochemical properties reveals enzyme impacts on composition. Higher dosages increase soluble solids up to 15% and soluble fiber content by 35% through cell wall breakdown. However, excessive amounts over 0.3% decrease yields. Pectin concentration also declines dose-dependently, falling by 91% at 0.4%, improving juice stability but needing modulation to retain viscosity. Electrochemical fingerprinting successfully differentiates process conditions, offering a rapid quality control tool. Its potential for commercial inline use during enzymatic treatment requires exploration. Overall, connecting optimized parameters to measured effects provides actionable insights for manufacturers to boost yields, determine enzyme impacts on nutrition/functionality, and introduce novel process analytical technology. Further investigations of health properties using these conditions could expand sea buckthorn juice functionality. [ABSTRACT FROM AUTHOR]

Strawberry (Fragaria × ananassa) is one of the most important fruit crops, worldwide. In June 2023, strawberry fruits with pink mould symptoms were collected from a storage facility in Khorasan Razavi province of Iran. Trichothecium roseum was isolated from symptomatic fruit and identified through morphological characteristics. Molecular identification was carried out based on the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) using ITS1 and ITS4 primers. Pathogenicity was confirmed according to Koch's postulates. Cell wall–degrading enzyme secretion was investigated as a potential factor in fungal pathogenicity. The results of morphological identification, ITS analysis and pathogenicity test indicated that the causal agent was T. roseum. Enzyme activity assays revealed that T. roseum obtained from strawberry with a high level of aggressiveness was able to secrete cellulase and pectinase. To our knowledge, this is the first report of post‐harvest pink mould of strawberry caused by T. roseum in Iran. [ABSTRACT FROM AUTHOR]

A dynamic plant architecture is the basis of plant adaptation to changing environments. Although many genes regulating leaf rolling have been identified, genes directly associated with water homeostasis are largely unknown. Here, we isolated a rice mutant, dynamic leaf rolling 1 (dlr1), characterized by ‘leaf unfolding in the morning-leaf rolling at noon-leaf unfolding in the evening’ during a sunny day. Water content was decreased in rolled leaves and water sprayed on leaves caused reopening, indicating that in vivo water deficiency induced the leaf rolling. Map-based cloning and expression tests demonstrated that an A1400G single base mutation in Oryza sativa Polygalacturonase 1 (OsPG1)/PHOTO-SENSITIVE LEAF ROLLING 1 (PSL1) was responsible for the dynamic leaf rolling phenotype in the dlr1 mutant. OsPG1 encodes a polygalacturonase, one of the main enzymes that degrade demethylesterified homogalacturonans in plant cell walls. OsPG1 was constitutively expressed in various tissues and was enriched in stomata. Mutants of the OsPG1 gene exhibited defects in stomatal closure and decreased stomatal density, leading to reduced transpiration and excessive water loss under specific conditions, but had normal root development. Further analysis revealed that mutation of OsPG1 led to reduced pectinase activity in the leaves and increased demethylesterified homogalacturonans in guard cells. Our findings reveal a mechanism by which OsPG1 modulates water homeostasis to control dynamic leaf rolling, providing insights for plants to adapt to environmental variation. [ABSTRACT FROM AUTHOR]

Bacterial isolates collected from the environment were screened for pectinolytic activity, and a strain with the highest activity was selected and identified as Bacillus subtilis Mz‐12. The presence of pectin hydrolase, lyase, and esterase activities was confirmed. Pectinase was purified and characterized. Enzyme production was optimized with respect to temperature, pH, and growth medium. Enzyme stability and activity were characterized under different temperatures and pH values. The results showed that this strain was capable of producing high yields of pectinase in commercial medium (Pharmamedia) 24.6 U/mL compared to other media. The purified pectinase of 22.3 kDa produced was constitutive in nature. The isolated enzyme from this strain displayed a wide range of temperature and pH stability, with the optimal activity observed at pH 9.0 and 50°C. These results indicate that the B. subtilis Mz‐12 strain is a favorable candidate for industrial enzyme production. The use of Pharmamedia is reported for first time for pectinase production. [ABSTRACT FROM AUTHOR]

Simple Summary: Inorganic minerals are often provided in excess in poultry diets to meet the nutritional needs of the birds, with phosphorus and calcium being of particular importance. Phosphorus, which is not easily digestible by birds, is already present in many of the common plant-based ingredients in poultry diets. This plant-based phosphorus can be made more digestible for birds through the inclusion of enzymes such as phytase. Other enzymes such as xylanase can also be included to help breakdown the plant-based material and release essential minerals and nutrients. In this study, the effects of adding a natural enzyme complex with primary activity of phytase and secondary activity of xylanase, pectinase, protease, β-glucanase, cellulase and amylase to broiler chicken diets were tested. The enzyme-supplemented birds showed improvements in various digestibility parameters. More phosphorus was retained in the enzyme-supplemented diets when compared to the positive control. These findings indicate that these enzymes can be used to improve the efficient use of phytate-bound phosphorus without negatively affecting broiler productivity. The use of enzymes also reduced phosphorus excretion, therefore reducing the potential negative impacts on the environment. The importance of enzymes in the poultry industry is ever increasing because they help to extract as many nutrients as possible from the raw material available and reduce environmental impacts. Therefore, an experiment was conducted to examine the effect of a natural enzyme complex (ASC) on diets low in AME, Ca and P. Male Ross 308 broilers (n = 900) were fed one of four diets: (1) positive control (PC) with no enzyme added (AME 12.55 MJ/kg, AVPhos 4.8 g/kg and AVCal 9.6 g/kg); (2) negative control (NC) with no enzyme added and reduced AME, Ca and P (AME 12.18 MJ/kg, AVPhos 3.3 g/kg, AVCal 8.1 g/kg); (3) negative control plus ASC at 200 g/t; and (4) negative control plus ASC at 400 g/t. Broiler performance, digesta viscosity, tibia mineralization and mineral content were analyzed at d 21. Between d 18 and 20, excreted DM, GE, total nitrogen, Ca, and P were analyzed. ASC at 200 g/t and 400 g/t improved the FCR (p = 0.0014) significantly when compared with that of the NC. There were no significant differences in BW or FI between the treatments. Birds fed ASC at 200 g/t and 400 g/t had significantly improved digesta viscosity (p < 0.0001) compared with that of the PC and NC birds and had significantly higher excreted DM digestibility (p < 0.01) than the NC and the PC birds with 400 g/t ASC. ASC inclusion significantly improved P retention (p < 0.0001) compared to that in the PC. Ca retention was significantly increased by 400 g/t ASC compared to that in the PC and NC (p < 0.001). AME was significantly higher (p < 0.0001) for all treatments compared to that in the NC. There were no significant differences between treatments for any of the bone measurements. This study showed that feeding with ASC can support the performance of broilers when fed a diet formulated to have reduced Ca, P and AME, with the greatest results being seen with a higher level of ASC inclusion. [ABSTRACT FROM AUTHOR]

Various studies have shown that the microbial proteins are often more stable than belongs to other sources like plant and animal origin. Hence, the interest in microbial enzymes has gained much attention due to many potential applications like bioenergy, biofuel production, biobleaching, bioconversion and so on. Additionally, recent trends revealed that the interest in isolating novel microbes from harsh environments have been the main focus of many scientists for various applications. Basically, industrially important enzymes can be categorized into mainly three groups: carbohydrases, proteases, and lipases. Among those, the enzymes especially carbohydrases involved in production of sugars. Carbohydrases include amylases, xylanases, pectinases, cellulases, chitinases, mannases, laccases, ligninases, lactase, glucanase, and glucose oxidase. Thus, here, an approach has been made to highlight five enzymes namely amylase, cellulase, laccase, pectinase, and xylanase from different sources with special emphasis on their properties, mechanism, applications, production optimization, purification, molecular approaches for its enhanced and stable production, and also biotechnological perspectives of its future development. Also, green and sustainable catalytic conversion strategies using nanoparticles of these enzymes have also been discussed. This review will provide insight into the carbohydrases importance and their usefulness that will help to the researchers working in this field. [ABSTRACT FROM AUTHOR]

Background: The demand for low-cost cellulolytic enzyme synthesis is rising in the enzyme market. This work aims to produce cellulase by utilizing various agricultural wastes and investigating the use of enzyme in saccharification and textile industries. Results: Solid state fermentation (SSF) was applied to produce industrial enzymes, particularly cellulase, through utilizing Molokhia (Corchorus olitorius) stems by Aspergillus awamori MK788209 isolate. Two stages of statistical factorial designs Plackett-Burman (PB) and Central Composite Design (CCD) were applied to enhance the A. awamori MK788209 cellulase production from Molokhia stems (MS). The fold increase of enzyme production by PB followed by CCD was 2.51 and 4.86, respectively. Additionally, the A. awamori MK788209 culture filtrate was highly effective in saccharifying various agricultural wastes, particularly pea peels (PP) (yielding 98.33 mg reducing sugar/ml), due to its richness in cellulase, laccase, xylanase, pectinase, and amylase. By optimizing the three main variables; pea peel weight, culture filtrate volume added, and saccharification time by CCD, the sugar recovery from PP was enhanced, leading to a 3.44-fold increase in reducing sugar recovery (338 mg reducing sugar /ml). Furthermore, the A. awamori MK788209 culture filtrate showed high efficacy in textile applications, enhancing the roughness, weight loss, white index, and printing capability of treated cotton fabrics. Conclusions: A. Awamori MK788209 produced cellulase which was effective in PP saccharification. The enzyme was also capable of enhancing cotton fabric properties. [ABSTRACT FROM AUTHOR]

Zingiber zerumbet affords the essential oil having important application and its rhizomes are the main ingredient in traditional Indian Ayurveda formulation. We report here the impact of enzymes on the isolable yield of oleoresin and essential oil (EO) from Zingiber zerumbet. The impact of enzymes like cellulase, amylase, bio-protease, pectinase, hemi-cellulase and enzyme for herbal extract is evaluated. Viscozyme affords the highest oleoresin (16%) and essential oil (7.1%), a betterment of 138% and 24% respectively. Zerumbone, a principal bioactive component of zerumbet, was afforded in 8.98% and 5.7% from oleoresin and essential oil. Box Behnken Design has been employed to optimize parameters to get the highest oleoresin and EO yield. The EO was tested for its inhibitory activity on α -amylase and α-glucosidase. The EO collected from viscozyme-assisted extraction inhibited α -amylase with IC 50 26 µM while the same from enzyme for herbal extract-assisted extraction inhibited α -glucosidase with IC 50 274 µM. The extraction efficiency of all the enzymes used here is reported. [Display omitted] • Viscozyme treated zerumbet affords high oleoresin & essential oil. • Zerumbone from oleoresin & essential oil was respectively 7.5% & 5.7%. • Zerumbet essential oil showed potent α -amylase and α -glucosidase inhibition. • Optimized the extraction method by RSM. • Quantified zerumbone by Gas Chromatography (GC). [ABSTRACT FROM AUTHOR]

Downy mildew caused by Sclerospora graminicola is a systemic infectious disease affecting foxtail millet production in Africa and Asia. S. graminicola-infected leaves could be decomposed to a state where only the veins remain, resulting in a filamentous leaf tissue symptom. The aim of the present study was to investigate how S. graminicola influences the formation of the filamentous leaf tissue symptoms in hosts at the morphological and molecular levels. We discovered that vegetative hyphae expanded rapidly, with high biomass accumulated at the early stages of S. graminicola infection. In addition, S. graminicola could affect spikelet morphological development at the panicle branch differentiation stage to the pistil and stamen differentiation stage by interfering with hormones and nutrient metabolism in the host, resulting in hedgehog-like panicle symptoms. S. graminicola could acquire high amounts of nutrients from host tissues through secretion of ß-glucosidase, endoglucanase, and pectic enzyme, and destroyed host mesophyll cells by mechanical pressure caused by rapid expansion of hyphae. At the later stages, .S', graminicola could rapidly complete sexual reproduction through tryptophan, fatty acid, starch, and sucrose metabolism and subsequently produce numerous oospores. Oospore proliferation and development further damage host leaves via mechanical pressure, resulting in a large number of degraded and extinct mesophyll cells and, subsequently, malformed leaves with only veins left, that is, "filamentous leaf tissue." Our study revealed the S. graminicola expansion characteristics from its asexual to sexual development stages, and the potential mechanisms via which the destructive effects of S. graminicola on hosts occur at different growth stages. [ABSTRACT FROM AUTHOR]

Pectinases are applied in many commercial processes, particularly for fruit juice clarification. Cost effective production of pectinase necessitates utilization of agro-industrial wastes as fermentation raw materials. Biomass from halophytic plants provides a prospect for its sustainable utilization in industrial processes. Here, we report utilization of two halophytic biomasses, Suaeda fruticosa and Cressa cretica to grow a pectinolytic strain, Geotrichum candidum AA15 and its mutant strain AHC1. Amount of pectinase produced was compared by cultivating the strains in the medium containing apple peels and orange peels. The data showed that G. candidum AHC1 can produce pectinase titers of 15.43 and 28.15 IU mL−1 within 25 h when cultivated in the medium containing biomass from C. cretica and S. fruticosa, respectively. The results of FTIR and Scanning Electron microscopy affirmed the bacterial growth mediated changes in the structure of the substrate that was associated with the pectinase production. The crude pectinase preparation was applied to hydrolyze an agro-waste, sugarcane bagasse, that resulted in the release of 86 mg g−1 of reducing sugars. Thus, this study proposed the usage of halophytic plant biomass for cost effective pectinase production and its application for saccharification of agro-waste. [ABSTRACT FROM AUTHOR]

A fundamental step in improving the quality and consumer acceptability of wild watermelon (Citrullus lanatus) is the process of juice clarification. The aim of this research was to investigate the physicochemical properties of crude wild watermelon (Citrullus lanatus) juice and to optimise the processing conditions, incubation time, incubation temperature and enzyme concentration for the enzymatic clarification of the crude juice. Crude wild watermelon juice samples were treated with pectinase enzyme in different concentrations (0.05 to 0.15 w/w%), at different incubation temperatures (30–50 oC) and for different incubation durations (60–180 min). The effects of the different treatments on turbidity, clarity, viscosity, lightness, and brix were determined. The response models adequately predicted turbidity, clarity, and viscosity at R2 > 0.5, but not lightness considering that R2 < 0.5. The model was statistically significant in predicting turbidity (R2 = 0.86), clarity (R2 = 0.81), viscosity (R2 = 0.97) and brix (R2 = 0.94) – but not lightness (R2 = 0.24) at p < 0.05. The enzyme concentration did not significantly affect turbidity, clarity, and lightness, but it did significantly affect brix positively (p < 0.05). Response surface methodology software was used to determine optimal clarification conditions. In conclusion, the optimum conditions for crude watermelon juice clarification were 0.15 w/w% enzyme concentration, 60 min incubation time and 60 oC incubation temperature. The optimum output parameters were 14.18 NTU for turbidity, 0.04 Abs for clarity, 52.30 L* value for lightness, 1.96 cps for viscosity and 3.08% for Brix. [ABSTRACT FROM AUTHOR]

Today, the extraction and use of biologically active compounds from agricultural and food wastes has received much attention. In the present research, the effect of enzyme treatment, ultrasound and the combined effect of enzyme treatment and ultrasound on the extraction of phenolic compounds of sour grape wastes was done so that 2 pectinase enzymes (Pectinex Ultracolor and Pectinex Yildamesh) at levels of 10, 20 and 30 mg/kg and ultrasound (times 10, 25 and 40 minutes) and (sound intensity 30, 60 and 90%) were used. The effect of enzyme treatment, ultrasound and the combined effect of enzyme treatment and ultrasound were investigated. According to the obtained results, the highest extraction efficiency belonged to Yaldemesh enzyme and with increasing sound intensity, the extraction efficiency increased significantly. On the other hand, increasing the extraction time led to an increase in extraction efficiency (p≤0.01). So that the highest extraction efficiency was observed in the sample extracted by Yaldemesh enzyme and under ultrasound at 90% sound intensity for 40 minutes (p≤0.01). According to the obtained results, the highest flavonoid, total phenol and antioxidant activity (DPPH, FRAP, ABTS) belonged to Yaldemesh enzyme, and with increasing sound intensity, antioxidant activity increased significantly (p≤0.01). On the other hand, increasing the extraction time led to a significant increase in antioxidant activity (p≤0.01). The highest antioxidant activity belonged to the sample extracted by Yaldemesh enzyme and subjected to ultrasound at a ultrasound intensity of 90% for 40 minutes and was introduced as the best treatment. [ABSTRACT FROM AUTHOR]

The demand for pectinases in the global market has been on the rise in recent years due to their significant biotechnological uses. The present study involves the characterization of a crude alkaline pectinase from Bacillus halodurans LBW 5117 and the demonstration of its bioscouring potential. The enzyme was an endo polygalacturonase (PGase LBW 5117) that was brown and had a slight fermentation odor. It exhibited an activity of 0.21 U/ml and retained 67, 90.5, 95.2, and 95.2 % of this activity after 1 year of storage at -20, 4, 20, and 30 °C, respectively. Its operational pH and temperature ranged between 10.2 - 11.0 (optimum, 10.5) and 45 - 57 oC (optimum 50 °C), respectively. Metal ions e.g., K+, Ca2+, Mg2+, Fe3+, Cu2+, Na+, Mn2+, and Zn2+ either stimulated or did not significantly affect its activity at 1 mM. The enzyme retained only 15 % of its activity after 8 h of incubation at 50 °C, but this improved to 200 % in the presence of 1.5 mM Ca2+ and 0.05 mM Tween 20. The enzyme was cellulase-free and hydrolyzed pectin in an endo-manner. Moreover, when used under the established optimized operating conditions, it degraded and eliminated pectin (and other non-cellulosic hydrophobic impurities that adhere to it) from woven cotton (weight loss = 0.68 %) and produced a more hydrophilic fabric with improved wettability for water (drop test = 10 sec) and dye (capillary rise test = 28 mm after 30 min). This result shows that PGase LBW 5117 possesses good operational properties, potentially making it a good bioscouring agent. It is, however, recommended that the bioscouring process be optimized, and its effectiveness be compared with that of standard bio-and/or chemical-scouring process(es). [ABSTRACT FROM AUTHOR]

The nutritional value of blue honeysuckle (Lonicera caerulea L.) fruit is abundant; however, its production faces challenges due to a short harvesting period and fruit drop issues. In this study, the effects and potential mechanisms of two different plant growth regulators, GA3 (Gibberellins acid) and 2,4-D (2,4-Dichlorophenoxyacetic acid), on blue honeysuckle fruit abscission and abscission layer formation were investigated. The main cultivated variety of blue honeysuckle, 'Berel', was used as the experimental material. GA3 and 2,4-D were sprayed on the plants from the veraison. The anatomical structure of the fruit abscission zone (FAZ) was observed after treatment. Key enzymes involved in cell wall degradation, pectinase, cellulase, polygalacturonase, and pectin methylesterase, were analyzed for their activities. Furthermore, the gene expression levels of cell wall detachment-related genes CX1, CX2, PL20, PE, and key genes for gibberellin and ethylene synthesis GA2OX1, GA1, ACO, and ACO3 were examined. The results indicated that the application of GA3 and 2,4-D could delay the formation of the abscission layer. In the FAZ treated with GA3 and 2,4-D at 5 DAT, the activity of key enzymes involved in cell wall degradation decreased, the expression of genes related to cell wall degradation enzymes and key genes for ethylene synthesis was inhibited, and the drop of fruit reduced. In conclusion, exogenous application of GA3 and 2,4-D suppresses the abscission of 'Berel' blue honeysuckle fruit, likely through the inhibition of cell wall degradation and abscission layer formation. [ABSTRACT FROM AUTHOR]

In this study, phytase from Bacillus megaterium EBD9-1 and cellulase from Bacillus subtilis 171ES obtained from isolated from different provinces soils, and commercial pectinase enzymes were investigated for the removal potential of plant impurities from raw wool fabrics. Enzymatic removal of plant impurities is usually carried out at pH 4.0. The efficiency of the enzyme was shown at pH 7.0, but the best effect was obtained with pH 4.0. Enzymes were effective alone, but the effects of double and triple enzyme mixtures on plant impurities were more. It was determined that the effect decreased in case the enzyme amount was used low, and the effect increased even more in the presence of the concentrated enzyme. With this study, which was carried out for the first time with local isolate, the operability of more environmentally friendly enzymes in carbonization processes traditionally performed with chemical substances was demonstrated. [ABSTRACT FROM AUTHOR]

Glycosidases are essential for the industrial production of functional oligosaccharides and many biotech applications. A novel β-galactosidase/α-L-arabinopyranosidase (PpBGal42A) of the glycoside hydrolase family 42 (GH42) from Paenibacillus polymyxa KF-1 was identified and functionally characterized. Using pNPG as a substrate, the recombinant PpBGal42A (77.16 kD) was shown to have an optimal temperature and pH of 30 °C and 6.0. Using pNPαArap as a substrate, the optimal temperature and pH were 40 °C and 7.0. PpBGal42A has good temperature and pH stability. Furthermore, Na+, K+, Li+, and Ca2+ (5 mmol/L) enhanced the enzymatic activity, whereas Mn2+, Cu2+, Zn2+, and Hg2+ significantly reduced the enzymatic activity. PpBGal42A hydrolyzed pNP-β-D-galactoside and pNP-α-L-arabinopyranoside. PpBGal42A liberated galactose from β-1,3/4/6-galactobiose and galactan. PpBGal42A hydrolyzed arabinopyranose at C20 of ginsenoside Rb2, but could not cleave arabinofuranose at C20 of ginsenoside Rc. Meanwhile, the molecular docking results revealed that PpBGal42A efficiently recognized and catalyzed lactose. PpBGal42A hydrolyzes lactose to galactose and glucose. PpBGal42A exhibits significant degradative activity towards citrus pectin when combined with pectinase. Our findings suggest that PpBGal42A is a novel bifunctional enzyme that is active as a β-galactosidase and α-L-arabinopyranosidase. This study expands on the diversity of bifunctional enzymes and provides a potentially effective tool for the food industry. [ABSTRACT FROM AUTHOR]

Freshwater fungi play an important role in the decomposition of organic matter of leaf litter in rivers and streams. They also possess the necessary mechanisms to endure lower temperatures caused by habitat and weather variations. This includes the production of coldactive enzymes and antifreeze proteins. To better understand the physiological activities of freshwater fungi in their natural environment, different methods are being applied, and genome sequencing is one in the spotlight. In our study, we sequenced the first genome of the freshwater fungus Filosporella fistucella (45.7 Mb) and compared the genome with the evolutionary close-related species Tricladium varicosporioides (48.2 Mb). The genomes were annotated using the carbohydrate-active enzyme database where we then filtered for leaflitter degradation-related enzymes (cellulase, hemicellulase, laccase, pectinase, cutinase, amylase, xylanase, and xyloglucanase). Those enzymes were analyzed for antifreeze properties using a machine-learning approach. We discovered that F. fistucella has more enzymes to participate in the breakdown of sugar, leaf, and wood than T. varicosporioides (855 and 719, respectively). Filosporella fistucella shows a larger set of enzymes capable of resisting cold temperatures than T. varicosporioides (75 and 66, respectively). Our findings indicate that in comparison with T. varicosporioides, F. fistucella has a greater capacity for aquatic growth, adaptability to freshwater environments, and resistance to low temperatures. [ABSTRACT FROM AUTHOR]

The fungal system holds morphological plasticity and metabolic versatility which makes it unique. Fungal habitat ranges from the Arctic region to the fertile mainland, including tropical rainforests, and temperate deserts. They possess a wide range of lifestyles behaving as saprophytic, parasitic, opportunistic, and obligate symbionts. These eukaryotic microbes can survive any living condition and adapt to behave as extremophiles, mesophiles, thermophiles, or even psychrophile organisms. This behaviour has been exploited to yield microbial enzymes which can survive in extreme environments. The cost-effective production, stable catalytic behaviour and ease of genetic manipulation make them prominent sources of several industrially important enzymes. Pectinases are a class of pectin-degrading enzymes that show different mechanisms and substrate specificities to release end products. The pectinase family of enzymes is produced by microbial sources such as bacteria, fungi, actinomycetes, plants, and animals. Fungal pectinases having high specificity for natural sources and higher stabilities and catalytic activities make them promising green catalysts for industrial applications. Pectinases from different microbial sources have been investigated for their industrial applications. However, their relevance in the food and textile industries is remarkable and has been extensively studied. The focus of this review is to provide comprehensive information on the current findings on fungal pectinases targeting diverse sources of fungal strains, their production by fermentation techniques, and a summary of purification strategies. Studies on pectinases regarding innovations comprising bioreactor-based production, immobilization of pectinases, in silico and expression studies, directed evolution, and omics-driven approaches specifically by fungal microbiota have been summarized. [ABSTRACT FROM AUTHOR]

Prickly pear has attracted domestic and international industries' attention owing to its nutritional and health benefit potentials. However, consumption of prickly pear juices is limited by many factors which are addressed essentially through processing methods. The purpose of this study was to compare the effect of clarification by pectinolytic enzymes of Penicillium occitanis coupled with microfiltration (MF) on the physicochemical properties and antioxidant activities of prickly pear juices. The juices were treated with various enzyme concentrations (0.5–5 U/mL), at different times (10–40 min) and temperatures (35–65 °C). The effect of these enzymatic treatments on the viscosity of juices was evaluated using a central composite design. The optimum conditions for the clarification of red fruit juice (RFJ) and green fruit juice (GFJ) were obtained based on surface and contour plots. The optimal treatment conditions were as follows: enzyme concentration 4.75 U/mL, time 37 min and temperature 55 °C for RFJ and enzyme concentration 4.65 U/mL, time 35 min and temperature 55 °C for GFJ. The experimental value of viscosity of the two prickly pear juices was reduced by 34.1% and 30%, respectively which was closely linked to the predicted value (31.79% and 28.93%, respectively). Higher permeate flux and better juice quality were achieved after the enzymatic treatment. The total phenolic and flavonoid contents were correlated with the antioxidant activities. During three months of storage at 4 °C, the clarified juices were microbiologically safe. Our findings showed that the combined pectinolytic enzymes-MF process can be considered as a promising technique that satisfactorily preserves the juice quality. [Display omitted] • Differences in the chemical composition of two prickly pear juices were observed. • The pectinase treatment conditions were optimized by RSM on the viscosity of juices. • The optimized treatment with pectinase was followed by microfiltration (MF). • Clear and stable prickly pear juices were obtained. [ABSTRACT FROM AUTHOR]

Premise: Current methods for maceration of plant tissue use hazardous chemicals. The new method described here improves the safety of dissection and maceration of soft plant tissues for microscopic imaging by using the harmless enzyme pectinase. Methods and Results: Leaf material from a variety of land plants was obtained from living plants and dried herbarium specimens. Concentrations of aqueous pectinase and soaking schedules were optimized, and tissues were manually dissected while submerged in fresh solution following a soaking period. Most leaves required 2–4 h of soaking; however, delicate leaves could be macerated after 30 min while tougher leaves required 12 h to 3 days of soaking. Staining techniques can also be used with this method, and permanent or semi‐permanent slides can be prepared. The epidermis, vascular tissue, and individual cells were imaged at magnifications of 10× to 400×. Only basic safety precautions were needed. Conclusions: This pectinase method is a cost‐effective and safe way to obtain images of epidermal peels, separated tissues, or isolated cells from a wide range of plant taxa. [ABSTRACT FROM AUTHOR]

Cucumber Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum seriously affects the sustainable development of the cucumber industry. In order to obtain high-efficiency antagonistic Bacillus spp. against cucumber Fusarium wilt and clarify its growth-promoting effect, hte biocontrol strain with a strong antagonistic effect against F. oxysporum were screened from Bacillus with a certain resistance to Fusarium solan, and its morphological, physiological and biochemical, genetic characteristics and plant growth-promoting characteristics were evaluated. The results showed that strain N1、N3 and N6 presented significant inhibitory effects on F. oxysporum, with inhibitory rates of 77.71%, 75.94% and 74.76%, respectively. Three strains were all identified as Bacillus velezensis, all the three strains showed releasing potassium ability, and produced protease, chitinase, pectinase and siderophores. In the seedlings disease-prevention and growth-promotion test, the disease index of inoculated strain N1, N3 and N6 significantly reduced, the control efficiency was 41.93%, 20.97%, and 66.13% respectively on the 18th day after inoculation. Inoculation with N1, N3 and N6 strains effectively promoted the growths of cucumber seedlings compared with single inoculation of F. oxysporum f. sp. treatment, including plant height, stem thickness, leaf area, chlorophyll content and aboveground and subsurface fresh weight, the growth promoting effect of N6 treatment was the most significant. It increased by 21.56%, 56.13%, 47.95%, 76.18%, 222.89% and 70% respectively. Compared with the control, the growth characteristics of treated plants significantly decreased except for the fresh weight of underground parts. Under inoculation F. oxysporum f. sp., the addition of N6 significantly increased the stem thickness and aboveground fresh weight. In conclusion, N6 can be used as potential biocontrol resources to control cucumber Fusarium wilt caused by F. oxysporum f. sp. cucumerinum and promote the growth of seedlings for sustainable and efficient cucumber production. [ABSTRACT FROM AUTHOR]

In the present study, attempts have been made to isolate reductive acetogens from the rumen fluid samples of Murrah buffaloes (Bubalus bubalis). Out of 32 rumen samples 51 isolates were isolated, and based on autotrophic growth for production of acetate and presence of formyltetrahydrofolate synthetase gene (FTHFS) 12 isolates were confirmed as reductive acetogens. Microscopic observations showed that ten isolates as Gram-positive rods (ACB28, ACB29, ACB66, ACB73, ACB81, ACB91, ACB133, ACB229, ACB52, ACB95) and two isolates as Gram-positive cocci (ACB19, ACB89). All isolates tested negative for catalase, oxidase, and gelatin liquefaction, whereas the production of H2S was detected for two (ACB52 and ACB95) of the above isolates. All these isolates showed autotrophic growth from H2 and CO2, and heterotrophic growth with different fermentable sugars, viz., d-glucose, D-fructose, and D-trehalose but failed to grow on salicin, raffinose, and l-rhamnose. Out of the isolates, two showed amylase activity (ACB28 and ACB95), five showed CMCase activity (ACB19, ACB28, ACB29, ACB73 and ACB91), three showed pectinase activity (ACB29, ACB52 and ACB89), whereas none of the isolates was found positive for avicellase and xylanase activity. Based on 16S rDNA gene sequence analysis, the isolates showed their phylogenetic relationship with maximum similarity up to 99% to different strains of earlier reported known acetogens of clostridia group including Clostridium sp. (6), Eubacterium limosum (1), Ruminococcus sp. (1) and Acetobacterium woodii (1) except one, i.e., Vagococcus fluvialis. The results indicate that reductive acetogens isolated from the rumen fluid samples of Murrah buffalos are both autotrophic and heterotrophic in nature and further investigations are required to exploit and explore their potential as an alternate hydrogen sink. [ABSTRACT FROM AUTHOR]

A naturally occurring component called pectin is present in many fruits, including berries and apples. Almost all plants contain it, where it adds to the structure of the cell. Pectinase is an enzyme product that is used professionally to break down pectin. Pectinases are widely used in the wine and citrus juice industries. It is used in the fruit juice business for clarification because it lowers viscosity, which results in the creation of clear juice. In the current research, soil samples were taken from six different regions in the Ahmedabad district in an effort to isolate bacteria that produce pectinase from those samples. Primary screening produced a total of 41 strains, of which 14 showed pectinase activity. Screening was done by using Vincent's agar medium containing pectin. Best pectinolytic activity was determined by clear zone of hydrolysis on selective media. Among 14 isolates, Isolate 1 was showing highest zone of utilization which was selected for further study. Pectinase was produced by submerge fermentation technique and physicochemical parameters were optimized in which isolate 1 showed highest activity at pH 7.4, temperature 37°C, incubation period 48hrs., inoculation size, substrate, carbon source, nitrogen source. Isolate 1 was characterized by its cultural, morphological, biochemical and molecular basis by 16S rRNA sequencing and designated as Bacillus subtilis. This isolate was applied for fruit juice clarification and demucilization. [ABSTRACT FROM AUTHOR]

This study evaluated the action of commercial and non-commercial cellulases and pectinases in the hydrolysis of soybean hulls (SH) and corn stover and cobs (CSC), the effect of temperature and agitation on the lignocellulosic substrate hydrolysis and the bromatological characteristics of hydrolyzed substrates. The effect of pretreatment on the hydrolysis of lignocellulosic residues and bromatological analysis were also evaluated. The highest hydrolytic activity occurred at 300 rpm for SH (47.95 and 51.43% for cellulase and pectinase, respectively) and at 350 rpm for CSC (26.05 and 9.23% for cellulase and pectinase, respectively). Non-commercial enzymes achieved 7.26–30% of the amount of hydrolysis obtained with commercial enzymes, on the same substrates. Pretreatment with 7.5% of NaOH and a particle size of the substrate of 0.5 mm significantly increased the hydrolysis of SH and CSC for both enzymes. The bromatological characteristics showed that soybean hulls hydrolyzed with both commercial cellulase and pectinase have potential for large-scale use in animal feed production. [ABSTRACT FROM AUTHOR]

Mangrove plants, also known as halophytes, are ecologically important plants that grow in various tropical and subtropical intertidal regions. Owing to the extreme abiotic and biotic stressful conditions they thrive in, these plants produce unique compounds with promising pharmacological propensities. Mangroves are inhabited by an astronomical number of fungal communities which produce a diverse array of extracellular degradative enzymes, namely: amylase, cellulase, xylanase, pectinase, cholesterol oxidase, etc. Such enzymes can be isolated from the mangrove fungi and harnessed for different biotechnological applications, for example, as replacements for chemical catalysts. Mangrove microbes attract considerable attention as they shelter the largest group of marine microorganisms that are resistant to extreme conditions and can produce novel biogenic substances. Vaccines developed from mangrove microbes may promise a safe future by developing effective immunization procedures with a minimum of economic burden. Interestingly, mangroves offer an exciting opportunity for synthesizing nanoparticles in a greener way as these plants are naturally rich in phytochemicals. Rhizophora mucronata Lam., Avicennia officinalis L. and Excoecaria agallocha L. are capable of synthesizing nanoparticles which have evolved recently as an alternative in various industries and are used for their biomedical application. Besides, the phytoconstituents isolated from mangrove plants, such as: gallic acid, galactose, lupeol, catechins, carotenoids, etc., were explored for various biological activities. These compounds are used in the pharmaceutical and nutraceutical industries to produce antimicrobial, antioxidant, anticancer, antidiabetic, and other therapeutic agents. The present review provides information on the biotechnological potentials of mangrove plants and their bioactive compounds as a new source of novel drugs, enzymes, nanoparticles and therapeutically important microbial pigments. Thus, this review forms a base of support and hasten the urgent research on biomedical applications of mangroves. [ABSTRACT FROM AUTHOR]

Lentinula edodes (shiitake mushroom) is one of the most widely cultivated edible mushrooms and is primarily cultivated using sawdust medium. While there have been improvements in the cultivation technology, the mechanism of mycelial block cultivation, such as mycelial growth and enzymatic sawdust degradation, has not been clarified. In this study, the mycelium was elongated longitudinally in the bottle sawdust culture for 27 days, and the cultivated sawdust medium was divided into three sections (top, middle, and bottom parts). To determine spatial heterogeneity in the enzyme secretion, the enzymatic activities of each part were analyzed. Lignocellulose degradation enzymes, such as endoglucanase, xylanase, and manganese peroxidase were highly secreted in the top part of the medium. On the other hand, amylase, pectinase, fungal cell wall degradation enzyme (β-1,3-glucanase, β-1,6-glucanase, and chitinase), and laccase activities were higher in the bottom part. The results indicate that the principal sawdust degradation occurs after mycelial colonization. Proteins with the laccase activity were purified from the bottom part of the medium, and three laccases, Lcc5, Lcc6 and Lcc13, were identified. In particular, the expression of Lcc13 gene was higher in the bottom part compared with the level in the top part, suggesting Lcc13 is mainly produced from the tip region and have important roles for mycelial spread and nutrient uptake during early stage of cultivation. [ABSTRACT FROM AUTHOR]

This study investigates the production of the enzyme cocktail by the isolated fungi Aspergillus flavus B2 (GenBank accession number OL655454) using agricultural and industrial (AI) residues as the sole substrate. Of all the AI residues tested, Jew's mallow stalk was the best inducer substrate for enzyme cocktail production without adding any nutrients. Statistical optimization using Response Surface Methodology enhanced the production by 5.45, 5.20, and 3.34-fold, respectively for pectinase, xylanase, and CMCase. Optimum temperature, activation energy (E a), and activation energy for denaturation (E d) were determined. Michaelis constant (K m) for CMCase, xylanase, and pectinase enzyme was 1.82, 1.23, and 1.05 mg/mL, respectively. Maximum reaction rate (V max) was 4.67, 5.29, and 17.13 U/mL, respectively for CMCase, xylanase, and pectinase. Thermal stability revealed that pectinase, CMCase, and xylanase enzymes retained 64.7%, 61.8%, and 53.2% residual activities after incubation for 1 h at 50 °C. Half-life time (t 0.5) of pectinase, CMCase, and xylanase at 50 °C were 189.38, 129.8, and 127.89 min, respectively. Thermodynamics of the produced enzymes enthalpy (ΔH* d), free energy (ΔG* d), and entropy (ΔS* d) were determined at 40, 50, and 60 °C. In the presence of EDTA (5 mM), CMCase, xylanase, and pectinase retained 69.5%, 66.2%, and 41.2%, respectively of their activity. This work is significant for the valorization of AI residues and the production of value-added products. • A new Aspergillus flavus B2 strain was isolated from sugarcane bagasse. • The new strain exhibited high CMCase, xylanase and pectinase production. • Jew's mallow stalk was the best inducer for co-production of enzymes. • Optimized media promote enzymes production in a range of 3.3–5.5-fold. • Produced enzymes utilizing wastes are suitable for industrial applications. [ABSTRACT FROM AUTHOR]

The interaction between endoarabinase (endoA) and exo-type enzymes has been studied during their joint hydrolysis of branched arabinane (BAra), sugar beet pulp (SBP), and apple pomace (AP). It has been shown that mixtures of endoA with arabinofuranosidase (AF) or arabinoxylan-arabinofuranhydrolase (AXH) with endoA contents of 20 and 40%, respectively, were the most effective in the hydrolysis of BAra. As a result of the optimization of the complex of arabinases, cellulases, and pectinase, almost complete conversion of AP into monosaccharides (arabinose, glucose, and fructose) has been carried out. During the hydrolysis of SBP, the conversion rate of hemicellulose (arabinane) was more than 50% and for cellulose, 75%. [ABSTRACT FROM AUTHOR]

Background: The process of producing enzymes from microorganisms is one of the most current approaches with a bright future. Pectinase is an acidic polysaccharide that is produced by microorganisms, accounts for a large proportion of commercially marketed enzymes and is extremely important because they are used in a variety of industrial, food and medical applications. This study aimed to use the peels of some fruits as a basic material in the production of pectinase enzyme by some fungi isolated from some fruits and vegetables via both solid-state and submerged fermentation methods. Methods: The current study included the isolation and identification of some fungi from fruits and vegetables in the local markets of Al-Diwaniyah City/Iraq, the detection of their capability to produce pectinase enzyme using the standard medium and the possibility of using the peels of some fruits as basic materials. Result: The results indicated the isolation and identification of 178 fungal isolates belonging to 11 genera at different frequency rates and it was found that the isolate P. expansum isolated from Apples was the most efficient isolate in producing pectinase enzyme and the use of Apple peels gave the highest production of the enzyme from the fungal isolate understudy compared to other fruit peels used. [ABSTRACT FROM AUTHOR]

This study aimed to assess the effectiveness of two clarifying procedures and their effects on some properties in thermally or microwave-pasteurized pomegranate juices. The experiment consisted in combining pectinase and protease as well as chitosan and gelatin once they were being stored in the refrigerator. The experiment consisted in three parts, being the first one a fresh juice without any clarification treatment, whereas, the second one was the fresh juice treated with clarifying agent consisted of pectinase and protease mixture ratio (2:1) at 0.75 v/v,and 50 °C for 20 min. Finally, the third one was fresh juice treated with chitosan and gelatin mixture at 0.4 and 0.8) g/L, respectively, at 50 °C for (20) min. The pasteurization of all three experiments was done by using two techniques, i.e., one with thermal water bath at 85 °C for two min and the microwave at 400 Watts for two min. All pomegranate juice bottles were stored at 4 °C for three months. The results showed a significant effect of the clarification method variable on the properties studied, especially turbidity, polyphenol and anthocyanin values. Moreover, the juice clarified with the enzymatic clarification method had better characteristics than the traditional ones during storage, what has therefore a better commercial appeal. The area of significance was founded with the use of traditional clarification with concentration at (0.4 and 0.8) g/l, and microwave pasteurization with 400 watts and zero month of storage at 4 °C, respectively, which is provided a minimum turbidity value. [ABSTRACT FROM AUTHOR]

The present research work was carried out on isolating lignocellulolytic enzyme-producing natural bacterial isolates of the Sundarban Mangrove ecosystem in West Bengal, India. Multiple bacterial isolations and qualitative as well as quantitative screening of the isolates was conducted by selective media plates and dye decolorization assay. Growth characterization of the top three isolates, namely GD1, GD2, and GD3, was determined in both nutrient broth and selective media. GD1 showed laccase activity in ABTS plate and decolorized congo red, malachite green, and methylene blue by 64.82%, 47.69%, and 33.33%, respectively, which is the inherent property of laccase. Along with the maximum laccase activity (9.72U/L), it also showed a little amount of cellulase (5.8U/ml) and pectinase (0.55U/ ml) activity in the enzyme assay. GD2 showed maximum cellulase activity (6.56U/ml) with only 3.3% degradation of congo red and 39.43% degradation of malachite green. Like GD1, GD2 also bears 5.6U/L laccase and 0.12U/ml pectinase enzyme activity. The last isolate, GD3 specialized in pectinase production and having 4.4U/ml enzyme activity, degraded congo red by 66.75% and malachite green by 54.26%. All isolates showed activity between 30° and 37°C, and pH ranges from 4.5 to 7. The outcomes of this research will be useful in the fiber industry like jute or banana, dye industry, pulp-paper industry, and textile industry for waste remediation, recycling, and fiber modification. [ABSTRACT FROM AUTHOR]

Pectin is a complex heteropolysaccharide with a predominantly galacturonic acid (GalA) main chain and various branching sugars, leading to some analytical and quantitative determination challenges. By comparison with various acid hydrolysis methods, an effective and precise hydrolysis method for GalA determination from pectin was investigated using a combination of pectinase hydrolysis (PH) and HPLC determination, which was named the PH-HPLC method. With a pectinase loading of 2250 U/g pectin, 4.0 g/L commercial pectin was almost completely hydrolysed to the intact and detectable GalA at 50 °C after 24 h, for quantitative determination by HPLC. Acid-catalysis methods showed obvious disadvantages in terms of GalA degradation or incomplete hydrolysis of pectin, resulting in imprecise determination results. Moreover, the PH-HPLC method was employed for the quantitative determination of GalA in three common natural pectin feedstocks and indicated 45.5–233.1% higher content of GalA than the acid hydrolysis method. Thus, the PH-HPLC method is demonstrated to be a precise approach for analysing and quantifying the GalA of pectin and respective feedstock. [ABSTRACT FROM AUTHOR]

Process parameters were optimized for enzyme (textile‐grade enzymes) hydrolysis of Azolla, water hyacinth, and algae biomass for recovery of maximum sugar to be used as feed for biohydrogen production by Enterobacter cloacae strain DT‐1. Under optimum temperature and pH, the hydrogen yield in mole per mole of sugar from enzyme‐hydrolyzed water hyacinth biomass, Azolla biomass, and Spirulina and Scenedesmus biomass was determined with regard to cellulase, hemicellulase, amylase, pectinase, and xylanase. The maximum hydrogen yield was observed with hemicellulase‐treated Spirulina, Azolla, and Scenedesmus biomass. [ABSTRACT FROM AUTHOR]

The mixed enzymes-assisted mechanical bio-extraction method was first used to extract polysaccharides from Dendrobium officinale. Different parameters including the ratio of enzyme, the amount of enzyme, the grinding time, the extraction time and the solid/liquid ratio were investigated by single factor experiments and multifactorial experiments. Through the response surface methodology the optimal extraction conditions were obtained with the ratio of cellulase to pectinase was 2: 1 and total amount of enzyme was 0.23 mg, the grinding time of 11.48 min, the extraction time of 5.99 min. The obtained polysaccharide extracts were hydrolyzed and derivatized and then injected into high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS) for monosaccharide composition analysis. After optimization of the chromatographic conditions (including mobile phase and column), twelve monosaccharides were successfully determined within 20 min. The proposed method provided satisfactory linearity with the correlation coefficients higher than 0.99, suitable recoveries (81.46–114.92 %), acceptable reproducibility ranging from 0.06 % to 4.77 %, low limits of detection (0.70–45.45 ng/mL). Compared with other methods, this method makes the extraction efficiency much higher and has the advantages of simple operation, environmental friendliness and mild extraction conditions. Therefore, this method can be used for the extraction of polysaccharides from plants and the determination of monosaccharides and has the potential to be used in more areas. [ABSTRACT FROM AUTHOR]

This research aimed to investigate the efficiency of crude xylanase–pectinase in pulping of sugarcane bagasse. Optimum biopulping was obtained, using xylanase–pectinase dose 200–60 IU/g, bagasse/liquid ratio 1:10 and 1.0% Tween 80 concentration at 55 °C temperature, pH 8.5 and period of treatment 180 min. Treatment of sugarcane bagasse samples with these enzymes generated pulp with lower rejections (58.76%), total solids (12.64%), kappa number (47.77%), higher screened pulp yield (10.66%), along with enhanced optical and physical properties, in comparison with a chemical pulp. Bagasse biopulping resulted in a 13% decrease in alkali dose to obtain the optical and physical properties similar to those achieved under the 100% alkali dose. The breaking length, burst factor, tear index, double fold, gurley porosity and viscosity were improved by 15.19, 37.64, 2.47, 37.77, 35 and 23.17%, respectively, after bleaching treatment of biopulped samples. Thus, enzymatic pulping is an eco-friendly environmentally sustainable approach, since it reduces the use of pulping chemicals and simultaneously improves the paper quality. This is the first report, showing pulping of sugarcane bagasse, with crude xylanase–pectinase, produced by an isolate. [ABSTRACT FROM AUTHOR]

【Objective】 The present study aimed to investigate the mechanism of allelopathy-promoting action of p-hydroxybenzoic acid on the pathogenicity of Fusarium oxysporum.【Method】 In the study, the root soil of Panax notoginseng with different planting years in the genuine producing area of Wenshan prefecture, Yunnan province was collected. The allelopathic effect of p-hydroxybenzoic acid on F. oxysporum was analyzed using HPLC, metagenomics combined with enzymology. 【Result】The soil planted P. notoginseng for 1, 2 and 3 years had varied p-hydroxybenzene acid contents, which were 3.48, 3.95 and 2.22 mmol/L, respectively. Under natural growth state, content of p-hydroxybenzoic acid in P. notoginseng planting soil decreased with planting time, while the population density of F. oxysporum increased. When the content of p-hydroxybenzoic acid in the soil was 2.61 mmol/L, the population density of F. oxysporum was only 20.94%. With the increase of p-hydroxybenzoic acid content, the population density of F. oxysporum in the soil decreased. When p-hydroxybenzoic acid content was 5.11 mmol/L, the density of F. oxysporum was only 1.057%. The medium concentration of p-hydroxybenzoic acid (2.5-5.0 mmol/L) promoted the growth of F. oxysporum, and its colony diameter was higher than that of the control. The colony was purple after 24 hours of culture, and the mycelial growth was slightly denser than that of the control. Moreover, a large number of conidia could be observed under the microscope, the activity of pectinase of F. oxysporum was promoted, which was 1.02 U/ (mL·min) when the concentration was 2.5 mmol/L. Under stress of 2.5-10.0 mmol/L p-hydroxybenzoic acid, the cellulase activity changed little. 【Conclusion】The optimal p-hydroxybenzoic acid concentration for promoting the allelopathy of F. oxysporum was about 2.0-3.0 mmol/L. At this concentration, the population density of F. oxysporum in soil was the highest, the colony diameter and spore yield reached the peak, and the secretion of pathogenic enzymes was promoted. [ABSTRACT FROM AUTHOR]

To increase its operational stability and ongoing reusability, B. subtilis pectinase was immobilized on iron oxide nanocarrier. Through co-precipitation, magnetic iron oxide nanoparticles were synthesized. Scanning electron microscopy (SEM) and energy dispersive electron microscopy (EDEX) were used to analyze the nanoparticles. Pectinase was immobilized using glutaraldehyde as a crosslinking agent on iron oxide nanocarrier. In comparison to free pectinase, immobilized pectinase demonstrated higher enzymatic activity at a variety of temperatures and pH levels. Immobilization also boosted pectinase's catalytic stability. After 120 h of pre-incubation at 50 °C, immobilized pectinase maintained more than 90% of its initial activity due to the iron oxide nanocarrier, which improved the thermal stability of pectinase at various temperatures. Following 15 repetitions of enzymatic reactions, immobilized pectinase still exhibited 90% of its initial activity. According to the results, pectinase's catalytic capabilities were enhanced by its immobilization on iron oxide nanocarrier, making it economically suitable for industrial use. [ABSTRACT FROM AUTHOR]

Introduction. The aim of this research was to study the effect of pretreatment of pumpkin seed with proteolytic, cellulolytic, and pectolytic enzymes on pressed oil production. Materials and methods. Enzymes, papain, pectinase, cellulase, pepsin, and cellulolytic enzyme mixture Viscozyme L were used in the study. The number of disrupted cells was determined by the amount of easily extractable pumpkin seed oil as result of immediate shaking. To study the cell microstructure, ultramicrotome slices of pumpkin seeds were treated with individual enzymes and enzyme mixtures. The antioxidant activity of the pumpkin seed oil was determined using (DPPH) 2,2-diphenyl-1-picrylhydrazylradical radical scavenging activity. Results and discussion. The evaluation of cell integrity by immediate hexane extraction (shaking method) showed that all samples treated with different enzymes had a higher oil yield, ranging from 33.2 to 34.1% of seed weight, than the control samples (32.1%). The number of disrupted cells in enzyme treated samples was also higher than the control (64.4%), ranging from 67.6 to 69.5%. The highest amount of damaged cells, 71.0 and 71.1%, was found in the samples treated with mixtures (a) pepsin, Viscozyme L, pectinase and (b) pepsin, cellulase, with the pepsin+ViscozymeL+pectinase mixture was by 7.0% higher than that of the control sample. The enzymatic pretreatment did not significantly affect the free fatty acid content, peroxide value, fatty acid, and phytosterols composition. The antioxidant activity expressed as DPPH radical scavenging capacity of the pressed oil obtained from enzymatically pretreated pumpkin seeds was by 2.7% higher than of control oil. Conclusion. Pretreatment of pumpkin seeds with a mixture of cellulolytic and proteolytic enzymes allowed increasing the yield of oil with high quality characteristics. [ABSTRACT FROM AUTHOR]

Peptones are one of the most expensive components of microbial culture media. The present study was conducted to test the usability of low-cost sheep wool peptone (SWP) as an organic nitrogen source in the production of six industrially important enzymes (lipase, amylase, tannase, pectinase, cellulase and invertase). SWP was prepared by alkaline hydrolysis and acid neutralization. Bacillus licheniformis and Aspergillus niger were selected as test microorganisms for enzyme production. To evaluate the efficacy of SWP in enzyme production, it was compared with commercial tryptone peptone (TP) in the shaking flask cultures of the test microorganisms. The optimum concentration of both SWP and TP was determined to be 8 g/L for the production of B. licheniformis-derived enzymes, but 6 g/L for the production of A. niger-derived enzymes. It was determined that SWP was superior to TP in the production of four enzymes (lipase, amylase, tannase and pectinase) of both B. licheniformis and A. niger. This is the first study about the usage of sheep wool protein hydrolysate (SWP) as an organic nitrogen source or a peptone in fermentative production of microbial enzymes. [ABSTRACT FROM AUTHOR]

The traditional extraction technique for hemp fibers results in high energy consumption, serious pollution, excessive degumming and strength loss. Herein, a cleaning and reusable biochemical degumming method is provided, which combines calcium alginate microspheres loaded with pectinase lyase (CA-PL) and a small number of chemical auxiliaries. This biochemical degumming method has a shorter reaction time and lower reaction temperature and is environmentally friendly. Complete degumming can be achieved by repeating this method two to three times. The effect of the number of uses of the biochemical degumming solution on the composition, mechanical properties and strength of the resulting hemp fibers was studied. The biochemical degumming solution could be reused three times; the extraction rates of cellulose after each degumming treatment were 77.09%, 76.94% and 70.61%, and the breaking strength was 64.3 cN dtex−1, 62.1 cN dtex−1 and 59.7 cN dtex−1, respectively. The results of repeated degumming treatment showed that the optimal number of recycles of the biochemical degumming solution was two; the degumming efficiency was higher and the cellulose extraction degree was better. [ABSTRACT FROM AUTHOR]

For the first time, a large-scale screening has been carried out of pectinolytic activity in Saccharomyces bayanus strains isolated in various regions of the world from fermentation processes and natural sources. The ability to secrete active endo-polygalacturonase was shown to be a feature of this species. Regardless of the source and place of isolation, S. bayanus var. uvarum, S. bayanus var. bayanus and S. eubayanus have the PGU1bPGU2bPGU3b genotype. According to phylogenetic analysis, the pectinase genes (PGU) of the hybrid brewer's yeast S. pastorianus are originated from the cryophilic S. bayanus yeast and not from S. cerevisiae. Five selected S. bayanus strains with the highest pectinolytic activity are promising for further molecular genetic studies and breeding of wine yeasts. [ABSTRACT FROM AUTHOR]

Pectinases (EC 3.2.1.15) are a class of enzymes that catalyze the depolymerization or de-esterification reactions that degrade pectic substances. in the present study, we have isolated the Aspergillus niger strain from soil samples, in india and evaluated pectinase production. the highest pectinase producing A. niger strain was further evaluated and optimized with various agricultural wastes. Plackett-Burman design (PBD) and Central composite design '(CCD)' were used to determine the best parameters for maximum pectinase production. Pectinase activity was increased to 99.21 U/ml after optimizing the production medium using PBD and CCD statistical analysis. A positive correlation of pectinase activity between predicted (112.65 U/ml) and experimental (99.21 U/ml with SD=0.005) optimum was observed. Maximum pectinase was produced by A. niger under submerged fermentation, utilizing orange peel, which is a cost-effective, adaptable, and environmentally friendly approach. the partially purified pectinase showed significant application for apple juice clarification and showed the ability to degrade pectin and therefore the colour change was observed in apple juice within 120 min. Maximum pectinase was produced by A. niger using agricultural waste orange peel under submerged fermentation which is an economical, versatile and eco-friendly process and pectinase showed a significant application for apple juice clarification. [ABSTRACT FROM AUTHOR]