1. ALKBH5 regulates chicken adipogenesis by mediating LCAT mRNA stability depending on m6A modification.
- Author
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Chao, Xiaohuan, Guo, Lijin, Ye, Chutian, Liu, Aijun, Wang, Xiaomeng, Ye, Mao, Fan, Zhexia, Luan, Kang, Chen, Jiahao, Zhang, Chunlei, Liu, Manqing, Zhou, Bo, Zhang, Xiquan, Li, Zhenhui, and Luo, Qingbin
- Subjects
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ADIPOGENESIS , *CHICKENS , *PYRUVATE dehydrogenase kinase , *RNA methylation , *ADIPOSE tissues , *MESSENGER RNA , *FAT cells - Abstract
Background: Previous studies have demonstrated the role of N6-methyladenosine (m6A) RNA methylation in various biological processes, our research is the first to elucidate its specific impact on LCAT mRNA stability and adipogenesis in poultry. Results: The 6 100-day-old female chickens were categorized into high (n = 3) and low-fat chickens (n = 3) based on their abdominal fat ratios, and their abdominal fat tissues were processed for MeRIP-seq and RNA-seq. An integrated analysis of MeRIP-seq and RNA-seq omics data revealed 16 differentially expressed genes associated with to differential m6A modifications. Among them, ELOVL fatty acid elongase 2 (ELOVL2), pyruvate dehydrogenase kinase 4 (PDK4), fatty acid binding protein 9 (PMP2), fatty acid binding protein 1 (FABP1), lysosomal associated membrane protein 3 (LAMP3), lecithin-cholesterol acyltransferase (LCAT) and solute carrier family 2 member 1 (SLC2A1) have ever been reported to be associated with adipogenesis. Interestingly, LCAT was down-regulated and expressed along with decreased levels of mRNA methylation methylation in the low-fat group. Mechanistically, the highly expressed ALKBH5 gene regulates LCAT RNA demethylation and affects LCAT mRNA stability. In addition, LCAT inhibits preadipocyte proliferation and promotes preadipocyte differentiation, and plays a key role in adipogenesis. Conclusions: In conclusion, ALKBH5 mediates RNA stability of LCAT through demethylation and affects chicken adipogenesis. This study provides a theoretical basis for further understanding of RNA methylation regulation in chicken adipogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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