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1. An experiment was conducted to determine the effect of the source of fat (soybean oil or tallow) on the ileal endogenous amino acid (EAA) losses in broilers.2. Three nitrogen (N)-free diets; a control diet with no added fat and test diets with 60 g/kg of either soybean oil or tallow were formulated. Titanium dioxide (5 g/kg) was added to all diets as an indigestible marker. Each diet was assigned to six replicate cages (eight birds per cage) from d 18 to 21 post-hatch. On d 21, the digesta were collected from the lower half of the ileum.3. The endogenous losses of nitrogen and amino acids (AA) were lower (p = 0.08; p = 0.001) in broilers fed diets with soybean oil or tallow, respectively, compared to those fed the diet with no fat. Source of fat had no influence (p > 0.05) on EAA losses.4. The most abundant AA in the ileal endogenous protein was glutamic acid, followed by aspartic acid, threonine, leucine, serine, valine and proline. In general, the concentrations of AA in the endogenous protein were lower (p < 0.05) with added fat. The exceptions were methionine, cysteine, proline and serine, which were unaffected. The effect of fat source on the AA contents of endogenous protein were inconsistent and differed depending on the AA.5. The inclusion of fats decreased EAA losses which implied they have beneficial effects beyond direct energy contribution. It can be proposed that the reduction of EAA flow may be an additional mechanism contributing to the extra-caloric effect of dietary fats. [ABSTRACT FROM AUTHOR]

In the current study, an electronic nose (E-nose) and gas chromatography–ion mobility spectrometry (GC-IMS) were employed to investigate the volatile flavor compounds (VFCs) of intense flavor beef tallow (L) and ordinary beef tallow (P). The study results indicate that an E-nose combined with an LDA and GC-IMS combined with an OPLS-DA can effectively distinguish between the two types of beef tallow. Compared with ordinary beef tallow, the E-nose sensors of intense flavor beef tallow have stronger response signals to sulfides, terpenes, and nitrogen oxides. A total of 22 compounds contribute to making the flavor of intense flavor beef tallow more typical and richer; in contrast, ethyl acetate was the main aroma-active compound found in the ordinary beef tallow. Sulfur-containing compounds and terpenoids might be the key substances that cause sensory flavor differences between the two types of beef tallow. In conclusion, the results of this study clarify the characteristics and differences of the two types of beef tallow and provide an enhanced understanding of the differences in the flavors of the two types of beef tallow. [ABSTRACT FROM AUTHOR]

The EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) assessed the safety of amines, di‐C14‐C18‐alkyl, oxidised, renamed by the Panel as amines, di‐C14‐C20‐alkyl, oxidised, from hydrogenated vegetable oil. The substance amines, bis(hydrogenated tallow alkyl) oxidised, consisting of the same components, but originating from tallow, is currently authorised as FCM substance No 768. The vegetable‐sourced substance is intended to be used at up to 0.1% w/w as antioxidant and/or stabiliser in the manufacture of polyolefin food contact materials (FCM) and articles intended for contact with dry, aqueous and acidic foods. The substance is a mixture consisting of linear N,N‐dialkyl hydroxylamines and their corresponding amine, nitrone and oxime derivatives, as well as further components: tert‐N‐oxides, secondary amides and carboxylic acids. Specific migration was tested from polyethylene samples in 10% ethanol and 3% acetic acid for 2 h at 100°C followed by 10 days at 60°C. None of the non‐authorised components were detected to migrate at detection limits (LoD) in the range 0.003–0.029 mg/kg. The LoD of authorised carboxylic acids was 0.35 mg/kg. The Panel reassessed the genotoxicity studies carried out on FCM No 768 and evaluated two new bacterial reverse mutation tests on the nitrone and oxime derivatives as well as new (qualitative/quantitative) structure–activity relationship (Q)SAR analyses on other components. The Panel concluded that the substance did not raise a concern for genotoxicity. The Panel concluded that the substance is not of safety concern for the consumers if it is used as an additive at 0.1% w/w in the manufacture of polyolefin FCM intended to be in contact with foods simulated by food simulants A, B, C and E, except for infant formula and human milk, for storage above 6 months at room temperature and below, including hot‐fill conditions and heating up to 100°C for 2 h. [ABSTRACT FROM AUTHOR]

旨在为复合风味牛油基火锅油产品的开发提供依据，以牛油和巴沙硬脂为原料，在不同温度（80、100、120 ℃）下熬制不同时间（20、40、60 min）制备牛油基火锅油，采用电子鼻和顶空固相微萃取气相色谱-质谱联用技术分析不同熬制条件下牛油基火锅油的挥发性风味物质组成与含量，以及其理化性质和感官品质，考察熬制条件对牛油基火锅油品质的影响。结果表明：火锅油中的挥发性风味物质主要由萜烯类、烃类、醇类、酯类、醛类、酮类及其他类化合物组成，挥发性风味物质总数量在79~109之间，关键挥发性风味物质有10种，主要为萜烯类和醛类化合物，其中不良风味物质(E)-2-壬烯醛对熬制温度为120 ℃的火锅油风味贡献最大，与未经过熬制的牛油-巴沙硬脂火锅油的相同；而以鸡香和鸡油味为风味特征的(E,E)-2,4-癸二烯醛对熬制温度为80、100 ℃的火锅油风味贡献最大，且在100 ℃下熬制60 min制备的火锅油的(E)-2-壬烯醛的相对气味活性值（ROAV）最低〔在检出（E）-2-壬烯醛的所有火锅油中〕；熬制条件对火锅油样脂肪酸组成及含量没有显著影响；不同熬制温度下熬制60 min内，火锅油的酸值（KOH）（< 1.40 mg/g ）无显著变化，而过氧化值在80、100 ℃下随熬制时间的延长先升高后降低，在120 ℃时呈下降趋势，但均符合DBS50/022—2021要求；120 ℃下熬制60 min的火锅油全氧化值和茴香胺值明显高于80 ℃和100 ℃的；随熬制时间的延长和熬制温度的升高，火锅油的颜色越深；经风味、色泽、外观、黏性/硬度等感官评价，100 ℃熬制60 min的火锅油总体感官得分较高。综上，在100 ℃下熬制60 min制备的牛油基火锅油的综合品质较佳，可作为适宜的牛油基火锅油的熬制条件。Aming to provide a basis for the development of multi-flavor tallow-based hotpot oil products,tallow-based hotpot oil was prepared by decoction of tallow and Pangasius bocourti stearin under different temperatures (80, 100, 120 ℃) for different times (20, 40, 60 min). Electronic nose and headspace solid phase microextraction-gas chromatography-mass spectrometry were used to analyzed the composition and content of volatile flavor substances, physicochemical properties and sensory quality of the tallow-based hotpot oil under different decoction conditions, so as to investigate the influence of decoction conditions on the quality of tallow-based hotpot oil. The results showed that the volatile flavor substances in hotpot oil were mainly composed of terpenes, hydrocarbons, alcohols, esters, aldehydes, ketones and other compounds, and the total number of volatile flavor substances was 79-109,in which ten kinds was key volatile flavor substances, mainly terpenes and aldehydes. The bad flavor substance (E) -2-nonenal contributed the most to the overall flavor of hotpot oil with a decoction temperature of 120 ℃, and it was the same as the hotpot oil without decoction.(E, E)-2, 4-decadienal characterized by chicken flavor and chicken oil flavor contributed the most to the overall flavor of hotpot oil with decoction temperature of 80, 100 ℃, and the lowest relative odour activity value(ROAV) of (E) -2-nonenenal was found in the hotpot oil prepared under the conditions of decoction time 60 min and decoction temperature 100 ℃ (among all hotpot oils in which (E)-2-nonenenal was detected).The decoction conditions had little influence on the fatty acid composition and content of the hotpot oil. The acid values of all the hotpot oils were less than 1.40 mgKOH/g within 60 min of decoction, the peroxide value increased first and then decreased with the increase of decoction time at 80 ℃ and 100 ℃, and showed a decreasing trend at 120 ℃, but both were in line with the requirements of DBS50/022-2021. The full oxidation value and anisidine value of the hotpot oil decocted at 120 ℃ for 60 min were significantly higher than that at 80 ℃ and 100 ℃. With the extension of decoction time and the increase of decoction temperature, the color of hotpot oil became darker. The sensory evaluation of flavor, color, appearance, viscosity/hardness showed that the overall sensory score of hotpot oil decocted at 100 ℃ for 60 min was higher. To sum up, the comprehensive quality of the tallow-based hotpot oil prepared at 100 ℃ for 60 min is better, which can be used as a suitable decoction condition of the tallow-based hotpot oil.

In most cases, the unused by-products of venison, including deer tallow, are disposed of in rendering plants. Deer tallow contains essential fatty acids and can be used to prepare products for everyday food and advanced applications. This work aimed to process deer tallow into hydrolyzed products using microbial lipases. A Taguchi design with three process factors at three levels was used to optimize the processing: amount of water (8, 16, 24%), amount of enzyme (2, 4, 6%), and reaction time (2, 4, 6 h). The conversion of the tallow to hydrolyzed products was expressed by the degree of hydrolysis. The oxidative stability of the prepared products was determined by the peroxide value and the free fatty acids by the acid value; further, color change, textural properties (hardness, spreadability, stickiness, and adhesiveness), and changes at the molecular level were observed by Fourier transform infrared spectroscopy (FTIR). The degree of hydrolysis was 11.8–49.6%; the peroxide value ranged from 12.3 to 29.5 µval/g, and the color change of the samples expressed by the change in the total color difference (∆E*) was 1.9–13.5. The conditions of enzymatic hydrolysis strongly influenced the textural properties: hardness 25–50 N, spreadability 20–40 N/s, and stickiness < 0.06 N. FTIR showed that there are changes at the molecular level manifested by a decrease in ester bonds. Enzymatically hydrolyzed deer tallow is suitable for preparing cosmetics and pharmaceutical matrices. [ABSTRACT FROM AUTHOR]

Biolubricants are oleochemical esters that are non-toxic to the environment, and they are preferred because of their high-performance activities in various automobile and industrial fields. In this study, chicken tallow has been explored as an alternative to a non-renewable source, which acts as a low-cost feedstock for synthesizing biolubricants in a two-step hydro esterification process catalysed by Candida rugosa lipase (CRL). The enzymatic hydrolysis of chicken tallow is optimized at different time intervals at 40 °C, and the maximum release of 160.2 µmol min−1 free fatty acids (FFAs) is observed at 100 min. The hydrolysed FFAs are characterized by the gas chromatography-mass spectrometry (GC-MS) analysis that has depicted the fatty acid methyl esters (FAMEs) composition of the tallow oil. The recovered FFA is then enzymatically esterified with trimethylolpropane (TMP) to produce TMP esters. Optimization studies are performed to explore the effect of factors such as time, temperature, amount of the enzyme, and TMP: FFA molar ratio for the esterification process. Thus, the optimal conditions for achieving a maximal hydroxyl group conversion of 60% during TMP ester production include incubation time (120 min), temperature (35 °C), lipase concentration (3% w/w), and TMP: FFA molar ratio (1:4). Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) studies confirm the synthesized TMP esters. Moreover, physicochemical properties such as viscosity, cloud point (CP), and calorific value (CV) are found to be 36 mm2/s, 7159.14 cal/g, and 20 °C, respectively. [ABSTRACT FROM AUTHOR]

Introduction: In the past studies, the beneficial effect of insoluble fiber in broiler feed has been well demonstrated. Studies have shown that moderate amounts of fiber in poultry diets lead to significant improvements in nutrient utilization efficiency. Depending on the type of dietary fiber (soluble or insoluble), the particle size plays a role in the growth and development of gizzard and improves the mixture of digestive substances with digestive secretions. On the other hand, nutritionists are trying to respond to the rapid growth needs of birds by increasing the energy concentration of diets. Fats are considered for this purpose, as their energy value is at least twice that of carbohydrates and proteins. At a young age, the secretion of bile acids and the activity of pancreatic lipase are low, and therefore, the ability to digest fat in young broilers is compromised. Some studies have shown that cellulose and oat hull as a source of insoluble fiber (3%) in the diet of broiler chickens have increased the apparent digestibility of crude fat in the ileum and increased the availability of energy for the bird. Therefore, the purpose of this research is to investigate the effects of sunflower hull with different particle sizes in diets containing different sources of fat on growth performance, digestibility of nutrients and the microbial population of the caecum of broiler chickens. Materials and methods: This experiment was conducted using 360 one-day-old Ross 308 broiler chickens in a completely randomized design with a 2×3 factorial arrangement for 42 days. Experimental treatments included: sunflower hulls (SFH) (without hulls, 3% with 1 mm particle size and 3% with 5 mm particle size) and different fat sources (3% tallow, 3% canola oil). Feed intake (FI) and body weight gain (BWG) of birds were recorded and feed conversion ratio (FCR) was calculated. To determine the ileal digestibility of nutrients, 3 g/kg of chromium oxide was fed to chickens on days 37-42 of the rearing period. On the 42nd day of rearing, the ileum contents of 2 birds were collected and stored in a freezer at -20◦C. To investigate the morphology of the small intestine, 5 cm of the duodenum, jejunum and ileum were taken. Cecal microbial population also determined at 42 days of age. The data obtained from the experiment were analyzed using SAS statistical software and GLM procedure. To compare means, Duncan's (1955) multi-range test was used at a significant level of 5%. Results and discussion: The results showed that, using of the coarse and fine SFH in the diet of broiler chickens increased FI and improved the FCR in the whole period of experiment (P<0.05). Canola oil improved FI and BWG compared to tallow (P<0.05). Apparent digestibility of crude protein and crude fat also increased by using of coarse SFH in the diet (P<0.05). The use of coarse SFH increased the villus height of duodenum and the villus height and thickness of ileum (P<0.05). Canola oil increased the villus height of ileum, but decreased the villus thickness compared to tallow treatment (P<0.05). The use of coarse SFH in the diet reduced the population of Escherichia coli bacteria in the cecum, and coarse SFH with different sources of fat increased the population of Lactobacillus(P<0.05). Conclusions: In general, the results of the present study showed that the addition of coarse sunflower hull to the diet could improve the growth performance of broilers by increasing the digestibility of crude protein, crude fat, Lactobacillus population, and reducing the cecal population of E. coli. Although its interaction with canola oil also had positive effects on the population of beneficial gut bacteria. [ABSTRACT FROM AUTHOR]

This study aimed to restructure beef tallow‐milk‐vegetable oils blends through the chemical and enzymatic interesterifications and to analyze some properties of interesterified lipids including peroxide value, free fatty acid and mono‐di‐triacylglycerol contents, fatty acid profile, and slip melting point. Moreover, the mid‐infrared spectra of the samples were obtained using Fourier transform infrared spectroscopy. The monounsaturated and polyunsaturated fatty acid contents of beef tallow increased by blending and interesterification reactions. The free fatty acid content of the enzymatically interesterified lipids was higher than that of the chemically interesterified fats. The slip melting point of the structured fats decreased after both blending and interesterification. A statistical model showed that infrared spectra combined with analytical data could discriminate the interesterified lipids from binary and ternary blends. It was found out that a 15% milk and 40% vegetable oil ratio in the initial blends yielded as end products which could be an alternative product of butterfat in the bakery industry after chemical interesterification. [ABSTRACT FROM AUTHOR]

When plants are sequentially attacked by multiple herbivores, herbivore identity and host specialization can greatly influence the patterns of herbivore–herbivore and plant–herbivore interactions. However, how prior herbivory and the resulting induced plant responses potentially affect subsequent herbivores deserves further investigation. In this study, we conducted a common‐garden experiment that manipulated sequential herbivory by the specialist caterpillar Gadirtha fusca Pogue (Lepidoptera: Nolidae) and the generalist caterpillar Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) on Chinese tallow, Triadica sebifera (L.) Small (Euphorbiaceae). We tested how prior exposure to herbivores with different levels of host specialization affected the performance of subsequently arriving con‐ and heterospecifics, as well as plant growth and defense responses under subsequent herbivory. We found that prior exposure to the specialist G. fusca facilitated the performance of subsequent conspecifics, resulting in a significant decrease in the growth (height and stem diameter at ground level) of tallow plants. However, prior exposure to the generalist S. litura did not affect the feeding of subsequent con‐ or heterospecifics or the growth of tallow plants. Sequential herbivory by specialist and generalist conspecifics resulted in lower levels of tannins and flavonoids, respectively, in leaves of tallow plants, whereas sequential herbivory by the two species did not affect the levels of tannins or flavonoids, compared to a single damage event. We conclude that herbivore species‐specific plant responses appear to be more important than herbivore identity or specialization in determining herbivore–herbivore interactions and plant responses to sequential herbivore attack. [ABSTRACT FROM AUTHOR]

This study was conducted in the poultry farm of the Department of Animal Production / College of Agriculture / University of Anbar, during the period from 43 to 54 week, with the aim of study the effect of adding Fennel seeds (Foeniculum vulgare L.) to the diets on the productive performance of laying hens Seventy-two laying hens of Lohmann Brown breed, aged 43 weeks were used in this experiment. The layer hens were distributed randomly in to six treatments with four replicates per treatment (3 hens/ replicate). The treatments were T1: (Positive control) based diet containing 1% hydrogenated vegetable fat, T2: (negative control) based diet containing 1% animal fat, while treatments T3, T4, T5 and T6 The based diet contained 1% animal fat + supplementation of 0.25, 0.50, 0.75 and 1% Fennel seeds powder sequentially. The results showed were significant (P≤0.05) differences in productive traits of addition treatments, the treatment T6 was significantly (P≤0.05) increased compared with other treatments in (Hen House Production%, egg weight and egg mass) and a significant improvement (P ≤0.05) in feed conversion ratio, while treatment T4 was significantly (P≤0.05) increased compared with other treatment.

Introduction: One of the most important problems in the sheep breeding industry is its low reproduction capacity. According to the advances made in the field of reproduction, new methods have led to the improvement of the reproduction process. One of the important and effective indicators in reproduction is the level of energy used in the diet during the reproductive season. The use of fat supplements in the diet increases the energy density, and by reducing the inhibitory effects of the negative energy balance, it improves reproductive and productive performance. The right amount of oil consumption guarantees health and food safety. It is recommended that 10% or less of the energy needed by the body per day comes from saturated fat sources and 20-35% of daily energy comes from unsaturated fat. Due to the importance of oil seeds, their cultivation is also very important and they have a wide cultivated area all over the world. The purpose of this research was to investigate the effects of levels and sources of oil and fat on reproductive performance and blood factors of Kurdish sheep.Materials and Methods: The experiment was conducted at the Research Unit Farm of the Light Livestock Breeding Shightogan Delaware Company, located in the southern Khorasan province, Iran. Fifty-six ewes with Non-pregnant with an average age of one year and average body weight of 41.35±2.5 kg were divided into 7 treatments and 8 replications for 60 days (habituation period 14 days). Experimental treatments include: 1- base diet without oil, 2- Basic ration containing 2% sunflower oil, 3- Basic ration containing 2% canola oil, 4- Basic ration containing 2% tallow, 5- Basic ration containing 4% sunflower oil, 6- Basic ration containing 4% canola oil and 7- Basic ration It contained 4% tallow. Estral synchronization was performed in spring with CIDR. Ewes after CIDR (14 day) extraction and PMSG injection as soon as the signs of estrus were observed, the ewes were isolated from the others and artificially inseminated with fresh sperm using the transcortical method. Energy and chemical composition of rations were similar and were examined simultaneously with CIDR removal and estrus observation. Then, in each experiment parameters such as the time of estrus initiation (hour), rate of return to estrus, parturition rate, rate of multiple births, number of lambs and rate of lambing were evaluated Body weight (BW) and body growth measures were recorded First and period End. Data obtained were analyzed by statistical software SAS (version 1.9). Estral synchronization was performed in spring with CIDR. Ewes after CIDR (14 day) extraction and PMSG injection as soon as the signs of estrus were observed, the ewes were isolated from the others and artificially inseminated with fresh sperm using the transcortical method. The nutrition program with software (SRNS) version was adjusted based on the pregnancy diet.Results and Discussion: The results showed that the highest pregnancy rate (100%) and lambing rate (125%) were related to ewes receiving rations with 2% tallow oil and 4% sunflower oil. The highest increase in body weight was observed in ewes consuming ration with 2% sunflower oil, which was significantly different from the control group. But with other groups, this difference was insignificant. The highest body weight gain and the best (lowest) feed conversion ratio were observed in ewes consuming 2% sunflower oil diet, which was significantly different from the control group. But with other groups, this difference was insignificant. Triglyceride concentration, plasma total protein concentration and plasma albumin were not affected by oil and fat sources in the diets and no significant difference was observed between the diets. There was a significant difference in the average apparent digestibility coefficients of dry matter, crude fat and organic matter of nutrients between treatments.Conclusion: In general, use of vegetable and animal oils in the diet can positive effect on the productive and reproductive performance of sheep results of this research showed that in order to reduce the rate of consumption costs, 2% in the ration is suggested.

Background: The digestibility of animal fats and oils is limited by a reduction in the production and secretion of lipase and bile salts in young chickens. The addition of a natural emulsifier (lysophospholipids [LPL]) in poultry diet may increase the emulsification of lipids and their digestibility. Objectives: An experiment was conducted to evaluate the effects of feed LPLs supplementation with different fat sources on performance, serum lipid composition, small intestine morphology and caeca microflora in broiler chickens. Methods: A completely randomized factorial design (2 × 3 × 2) was used to evaluate the effect of LPL supplementation (0 and 0.25 g/kg) and three different fat sources (soybean oil, tallow and a 50:50 mixture of the two) in corn and soybean meal diets containing two levels of fat (1.5 and 3%), providing 12 isocaloric and isonitrogenous grower diets. Each experimental diet was fed to six replications of 10 birds from 15 to 28 days of age. Average growth performance during this period and small intestine morphology, serum lipid composition and caeca microflora were evaluated on day 28. Results: The interaction effects of LPL supplementation, source and/or level of fats were not significant for the performance parameters measured during the 15 to 28 days. The treatment effects were significant for the villus width and crypt depth measured in the jejunum on day 28. The LPL supplementation significantly increased crypt depth. The interaction effect of fat source and level of fat were significant for villus width. The addition of a 3% blend of soybean oil/tallow (50/50) reduced the serum low‐density lipoprotein (LDL) level. The Lactobacillus population was increased by the addition of LPL, or a 1.5% blend of soybean oil and tallow, to the diet. Conclusions: Our study showed that LPL supplementation of diet containing a 1.5% blend of soybean oil and tallow can improve serum lipid indices and caeca Lactobacillus populations in broiler chickens. [ABSTRACT FROM AUTHOR]

Introduction:1 One of the most important problems in the sheep breeding industry is its low reproduction capacity. According to the advances made in the field of reproduction, new methods have led to the improvement of the reproduction process. One of the important and effective indicators in reproduction is the level of energy used in the diet during the reproductive season. The use of fat supplements in the diet increases the energy density, and by reducing the inhibitory effects of the negative energy balance, it improves reproductive and productive performance. The right amount of oil consumption guarantees health and food safety. It is recommended that 10% or less of the energy needed by the body per day comes from saturated fat sources and 20-35% of daily energy comes from unsaturated fat. Due to the importance of oil seeds, their cultivation is also very important and they have a wide cultivated area all over the world. The purpose of this research was to investigate the effects of levels and sources of oil and fat on reproductive performance and blood factors of Kurdish sheep. Materials and Methods: The experiment was conducted at the Research Unit Farm of the Light Livestock Breeding Shightogan Delaware Company, located in the southern Khorasan province, Iran. Fifty-six ewes with Non-pregnant with an average age of one year and average body weight of 41.35±2.5 kg were divided into 7 treatments and 8 replications for 60 days (habituation period 14 days). Experimental treatments include: 1- base diet without oil, 2- Basic ration containing 2% sunflower oil, 3- Basic ration containing 2% canola oil, 4- Basic ration containing 2% tallow, 5- Basic ration containing 4% sunflower oil, 6- Basic ration containing 4% canola oil and 7- Basic ration It contained 4% tallow. Estral synchronization was performed in spring with CIDR. Ewes after CIDR (14 day) extraction and PMSG injection as soon as the signs of estrus were observed, the ewes were isolated from the others and artificially inseminated with fresh sperm using the transcortical method. Energy and chemical composition of rations were similar and were examined simultaneously with CIDR removal and estrus observation. Then, in each experiment parameters such as the time of estrus initiation (hour), rate of return to estrus, parturition rate, rate of multiple births, number of lambs and rate of lambing were evaluated Body weight (BW) and body growth measures were recorded First and period End. Data obtained were analyzed by statistical software SAS (version 1.9). Estral synchronization was performed in spring with CIDR. Ewes after CIDR (14 day) extraction and PMSG injection as soon as the signs of estrus were observed, the ewes were isolated from the others and artificially inseminated with fresh sperm using the transcortical method. The nutrition program with software (SRNS) version was adjusted based on the pregnancy diet. Results and Discussion: The results showed that the highest pregnancy rate (100%) and lambing rate (125%) were related to ewes receiving rations with 2% tallow oil and 4% sunflower oil. The highest increase in body weight was observed in ewes consuming ration with 2% sunflower oil, which was significantly different from the control group. But with other groups, this difference was insignificant. The highest body weight gain and the best (lowest) feed conversion ratio were observed in ewes consuming 2% sunflower oil diet, which was significantly different from the control group. But with other groups, this difference was insignificant. Triglyceride concentration, plasma total protein concentration and plasma albumin were not affected by oil and fat sources in the diets and no significant difference was observed between the diets. There was a significant difference in the average apparent digestibility coefficients of dry matter, crude fat and organic matter of nutrients between treatments. Conclusion: In general, use of vegetable and animal oils in the diet can positive effect on the productive and reproductive performance of sheep results of this research showed that in order to reduce the rate of consumption costs, 2% in the ration is suggested. [ABSTRACT FROM AUTHOR]

Copyright of Anbar Journal of Agricultural Sciences is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

为解决牛油火锅底料夏季软化形变及轻微化油问题，对酯交换法制备的高硬度牛油的基本理化指标、脂肪酸组成及含量、硬度、固体脂肪含量（SFC）进行了分析，并与老火锅牛油、混合牛油（由棕榈油和老火锅牛油以质量比1∶ 1混合而成）进行了对比。结果表明：3种牛油在基本理化指标上存在一定差异；3种牛油脂肪酸组成一致，各脂肪酸含量有明显差异；4 ℃时混合牛油的硬度最高，20～40 ℃时高硬度牛油的硬度最高，且随着温度的升高，高硬度牛油的硬度变化最小；温度超过10 ℃时，高硬度牛油的SFC最高，且随着温度的升高，其SFC下降速率最低。综上，高硬度牛油可以更好地保持牛油火锅底料在货架期的外观形态。In order to solve the problems of softening deformation and slight oil melting of beef tallow hotpot seasoning in summer, the basic physicochemical indexes, fatty acid composition and content, hardness and solid fat content (SFC) of high hardness beef tallow prepared by transesterification were analyzed and compared with old hotpot beef tallow and mixed beef tallow (mixed by palm oil and old hotpot beef tallow at a mass ratio of 1∶ 1). The results showed that there were some differences in the basic physicochemical properties of the three types of beef tallow. The fatty acid composition of the three types of beef tallow was consistent, but the content of each fatty acid was significantly different. The hardness of the mixed beef tallow was the highest at 4 ℃, and the hardness of the high hardness beef tallow was the highest at 20-40 ℃. With the increase of temperature, the hardness of the high hardness beef tallow changed the least. The SFC of the high hardness beef tallow was the highest over 10 ℃, and the SFC decline rate was the lowest with the increase of temperature. In summary, high hardness beef tallow can better maintain the appearance of the beef tallow hotpot seasoning during the shelf life.

In the current study, an electronic nose (E-nose) and gas chromatography–ion mobility spectrometry (GC-IMS) were employed to investigate the volatile flavor compounds (VFCs) of intense flavor beef tallow (L) and ordinary beef tallow (P). The study results indicate that an E-nose combined with an LDA and GC-IMS combined with an OPLS-DA can effectively distinguish between the two types of beef tallow. Compared with ordinary beef tallow, the E-nose sensors of intense flavor beef tallow have stronger response signals to sulfides, terpenes, and nitrogen oxides. A total of 22 compounds contribute to making the flavor of intense flavor beef tallow more typical and richer; in contrast, ethyl acetate was the main aroma-active compound found in the ordinary beef tallow. Sulfur-containing compounds and terpenoids might be the key substances that cause sensory flavor differences between the two types of beef tallow. In conclusion, the results of this study clarify the characteristics and differences of the two types of beef tallow and provide an enhanced understanding of the differences in the flavors of the two types of beef tallow.

The construction industry represents the largest energy-consuming sector globally, primarily due to its substantial operational processes power demand. To address this, there has been an increased emphasis on using materials capable of absorbing and storing heat as alternative energy storage in buildings. Phase Change Materials (PCMs) demonstrate this capability, harnessing the latent heat principle to absorb surplus heat energy and subsequently release it in times of deficiency. This study examines the mechanical and thermal properties of wall cladding materials integrated with PCMs, specifically beef tallow and rice husk charcoal. These composites were produced via direct incorporation, with rice husk charcoal weight fractions of 8, 10, and 12 %. This approach resulted in weight fractions of 28, 30, and 32 % in the plaster layer material. Fourier Transform Infrared Spectroscopy (FTIR) tests confirmed the PCM composite's chemical compatibility across all its components, with the composite morphology appearing as microcapsules. In terms of thermal conductivity, the addition of rice husk charcoal to beef tallow enhanced the PCM composite's performance. This enhancement indicated that approximately 10 % of rice husk charcoal weight fraction could be successfully incorporated into the plaster layer material without leakage. At an ambient temperature of 45 ℃, a plaster composite with 30 wt. % PCM met the standard compressive strength for plaster coating. Furthermore, it was found that this composite could reduce the temperature by 2.4 ǃ. The results concluded that beef-tallow PCM exhibits promising potential as a heat storage system for buildings, contributing to energy conservation in the construction industry. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the quality of hotpot oil from various hotpot seasonings. For this, 12 representative hotpot seasonings with beef tallow (BT) and flavored rapeseed oil (FRO) were collected before the hotpot oil was extracted. The oil content, sensory evaluation scores, physiochemical properties, fatty acid composition, harmful substances, and nutrient content of the hotpot oil were subsequently analyzed. The results showed that the oil content of the hotpot seasoning was 38.3%–58.2%. Furthermore, the BT hotpot oils produced better sensory scores (7–8.5), and their oxidative stability (12.08–13.17 h) was higher on average than that of the FRO hotpot oils. Additionally, the FRO hotpot oils had higher contents of unsaturated fatty acid (81.70%–97.32%), phytosterol (3466.07–6110.37 ppm), tocopherol (182.91–1276.17 mg kg−1), and polyphenol (34.48–61.94 mg kg−1). The factor analyses revealed that the FRO and BT hotpot oils were significantly different and were affected by the iodine value, acid value, and linoleic acid and phytosterol contents. Practical applications: It is necessary to improve the nutritional value and taste of hotpot oils to facilitate rapid development in the hotpot seasoning industry. This study showed FRO was a positive mediator of antioxidant, anti‐inflammatory, and anticancer effects owing to its richness of nutritional compounds, such as polyphenols, phytosterols, and tocopherols. In comparison, BT was found to have a lower nutritional value than FRO but added a unique taste and aroma to the hotpot. The use of blended oil as raw oil could also improve the quality of hotpot oil. This information will provide an important guide to the nutritional value and industrial production of hotpot oil. Blended oil is a promising raw oil for future use in hotpot seasoning processing to meet consumer demands for nutritious and pleasantly flavored hotpot oil. [ABSTRACT FROM AUTHOR]

Abstract Background The digestibility of animal fats and oils is limited by a reduction in the production and secretion of lipase and bile salts in young chickens. The addition of a natural emulsifier (lysophospholipids [LPL]) in poultry diet may increase the emulsification of lipids and their digestibility. Objectives An experiment was conducted to evaluate the effects of feed LPLs supplementation with different fat sources on performance, serum lipid composition, small intestine morphology and caeca microflora in broiler chickens. Methods A completely randomized factorial design (2 × 3 × 2) was used to evaluate the effect of LPL supplementation (0 and 0.25 g/kg) and three different fat sources (soybean oil, tallow and a 50:50 mixture of the two) in corn and soybean meal diets containing two levels of fat (1.5 and 3%), providing 12 isocaloric and isonitrogenous grower diets. Each experimental diet was fed to six replications of 10 birds from 15 to 28 days of age. Average growth performance during this period and small intestine morphology, serum lipid composition and caeca microflora were evaluated on day 28. Results The interaction effects of LPL supplementation, source and/or level of fats were not significant for the performance parameters measured during the 15 to 28 days. The treatment effects were significant for the villus width and crypt depth measured in the jejunum on day 28. The LPL supplementation significantly increased crypt depth. The interaction effect of fat source and level of fat were significant for villus width. The addition of a 3% blend of soybean oil/tallow (50/50) reduced the serum low‐density lipoprotein (LDL) level. The Lactobacillus population was increased by the addition of LPL, or a 1.5% blend of soybean oil and tallow, to the diet. Conclusions Our study showed that LPL supplementation of diet containing a 1.5% blend of soybean oil and tallow can improve serum lipid indices and caeca Lactobacillus populations in broiler chickens.

The moisture distribution and key volatile flavor compounds of beef tallow from Qinchuan cattle roasted for different periods were analyzed by low field-nuclear magnetic resonance (LF-NMR) spectroscopy and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). The results showed that the L* value significantly decreased (P 0.05), while the proportion of immobile water first increased and then decreased, and the proportion of free water showed an overall downward trend (P > 0.05). The relaxation time T23 and T24 showed a significant decreasing trend as roasting time increased (P < 0.05), indicating that the binding between water molecules and macromolecular substances such as protein and lipid became tighter. Beef tallow samples roasted for different periods could be effectively distinguished by electronic nose and the roasting process had a significant effect on the contents of alcohols, aromatic compounds, terpenes, and organic sulfur compounds in beef tallow according to principal component analysis (PCA) of the electronic nose data. A total of 95 volatile compounds were identified in all samples. The contents of 1-methoxy-2-propanol, 1-octene-3-ol, hexanal, nonanal, acetic acid, nonanoic acid, and acetamide were relatively higher in each sample, whereas ketones, heterocycles and amine compounds occurred mostly in the later stages of roasting. In total 19 key aroma components were identified based on odor activity value (OAV) and most of the substances have the largest OAV at 12 min of roasting.

To smelt beef tallow by using 1 000 kg as the mass of each sample of beef fat, and the fatty acid composition of the obtained butter including perirenal fat, abdominal fat and segmented fat was analyzed by gas chromatography (GC), and the volatile flavor components were detected by gas chromatography-olfactory mass spectrometry (GC-O-MS). The results showed that the saturated fatty acids (SFA) of the three kinds of beef tallow were mainly palmitic acid and stearic acid, and the unsaturated fatty acids (UFA) were mainly oleic acid. In addition, there was no significant difference (P>0.05) in palmitic acid content among three kinds of beef tallow. Although there were no significant differences in the stearic acid and oleic acid contents between perirenal tallow and belly tallow (P>0.05), there were significant differences in the stearic acid and oleic acid contents between partitioned tallow and waist oil and belly tallow (P

In most cases, the unused by-products of venison, including deer tallow, are disposed of in rendering plants. Deer tallow contains essential fatty acids and can be used to prepare products for everyday food and advanced applications. This work aimed to process deer tallow into hydrolyzed products using microbial lipases. A Taguchi design with three process factors at three levels was used to optimize the processing: amount of water (8, 16, 24%), amount of enzyme (2, 4, 6%), and reaction time (2, 4, 6 h). The conversion of the tallow to hydrolyzed products was expressed by the degree of hydrolysis. The oxidative stability of the prepared products was determined by the peroxide value and the free fatty acids by the acid value; further, color change, textural properties (hardness, spreadability, stickiness, and adhesiveness), and changes at the molecular level were observed by Fourier transform infrared spectroscopy (FTIR). The degree of hydrolysis was 11.8–49.6%; the peroxide value ranged from 12.3 to 29.5 µval/g, and the color change of the samples expressed by the change in the total color difference (∆E*) was 1.9–13.5. The conditions of enzymatic hydrolysis strongly influenced the textural properties: hardness 25–50 N, spreadability 20–40 N/s, and stickiness < 0.06 N. FTIR showed that there are changes at the molecular level manifested by a decrease in ester bonds. Enzymatically hydrolyzed deer tallow is suitable for preparing cosmetics and pharmaceutical matrices.

This research aimed to perform a lipidomics study using liquid chromatography-high resolution mass spectrometry (LC-HRMS) to identify lard, beef tallow and chicken fat. A total of 292, 345, and 403 lipid compounds were observed in lard, beef tallow, and chicken fat, respectively. The lipid groups of AcHexStE (acyl hexosyl stigmasterol ester), biotinylPE (biotinylphosphoetanolamine), LPC (lysophosphatidylcholine), MePC (monoetherphosphatidylcholine), PC (phosphatidylcholine) and PI (phosphoinocitol) were found to be specific for lard. The principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) successfully differentiated lard from beef tallow and chicken fat. This research suggested that the untargeted lipidomics technique using LC-HRMS combined with chemometrics could be used to discriminate lard from beef tallow and chicken fat. This method is a promising technique for the detection of lard adulteration in beef tallow and chicken fat for halal authentication purposes. [ABSTRACT FROM AUTHOR]

Using emulsifiers in poultry diets may increase digestibility of alternative lipid sources and enrich poultry egg yolks altering their fatty acid composition. This study aimed to evaluate performance, nutrient digestibility, egg quality, egg quality according to their storage period, and the fatty acid profile of Japanese quail egg yolk (Coturnix coturnix japônica). The design was completely randomized, in which 270 female quails were allocated in a 3x2 factorial diet arrangement: three lipid sources (soybean oil, poultry fat, and beef tallow), supplemented or not with an emulsifier, following two nutritional strategies – a diet formulated to meet the nutritional requirements proposed by INRA and a diet formulated with a reduction of 96 Kcal/kg of feed and added emulsifier. The effects of interactions between the lipid sources and the emulsifier were studied, their isolated effects when interactions were absent and the effect of storage time, and their interactions with the factors evaluated for egg quality variables. Feed intake was greater when beef tallow was added to the diets, while feed conversion was worse for birds fed diets with soybean oil and the emulsifier. Quails fed the diet with the emulsifier and soybean oil produced better quality eggs represented by the higher Haugh unit. Diets with beef tallow and poultry fat provided a higher percentage of palmitic and unsaturated fatty acids in the quail egg yolk. Alternative lipid sources such as beef tallow and poultry fat can be used as a substitute for soybean oil with added emulsifiers in diets for egg-laying quails without impairing performance and egg quality. The use of emulsifiers with alternative lipid sources to soybean oil can be considered a nutritional strategy in laying quail diets, but the reduction of energy in the diet must be adequate for the species. [ABSTRACT FROM AUTHOR]

The aims of this study were to investigate the recovery of antioxidant extract from oat (Avena Sativa L.) seed via ultrasonic waves and evaluate the antioxidative effect of the extract in tallow fat. The powdered oat seed sample was extracted with ethanol/H2O (70:30, V/V) using ultrasonic waves at the temperature of 50°C and time of 30 minutes with the frequency of 40 KHZ and solid to solvent ratio of 1:20 (w/v) in an ultrasound water bath. The extractive yield (24.20 ± 0.08 g/100g DW), total phenolic contents (365.73 ± 5.22 mg GAE/100g DW) and DPPH radical scavenging activity at different concentrations of oat extract (25 ppm, 50 ppm, 75 ppm, 100 ppm, 125 ppm, 150 ppm) were determined. The yielded extract was added to tallow. The protective effects of the extracts in stabilization of tallow fat at different concentrations (0 ppm, 400 ppm, 800 ppm, 1200 ppm) were evaluated daily for a period of five days (120 h) by monitoring the peroxide, p-anisidine, totox values at an oven temperature of 90C (accelerated oxidation). Also, the oxidative stability index (OSI) was evaluated and compared with the synthetic antioxidant BHT (200 ppm). In oxidative stability tests of tallow fat supplemented with oat antioxidant extracts, increasing the concentration of the extract led to its better performance in delaying oxidative rancidity (p <0.5). According to the results, BHT (200 ppm) and followed by the oat extract (1200 ppm) showed the highest protective effects. In the rancimat test (at120°C), the oxidative stability indices of the control sample, the sample of tallow fat containing 1200 ppm of oat extract and BHT (200 ppm) were 2.89, 5.86 and 6.91 hours, respectively (p <0.05). Thus, the antioxidant BHT and subsequent oat extract (1200 ppm) in comparison with the control sample, showed a significant effect on increasing the oxidative stability index and of course the induction period of tallow fat. According to the results of antioxidant extract of oats extracted by ultrasound can be used as a source of antioxidant compounds in edible oils. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Enzymatic interesterification (EIE) of beef tallow (BT) blended with soybean oil (SO), peanut oil (PO) and canola oil (CO) in different proportions were analyzed in terms of the fatty acid compositions, thermal properties (melting and crystallization behaviors), rheological properties, hardness, crystal, and microstructure to achieve their sufficient utilization. The content of saturated fatty acids in all interesterified oils (IOs) decreased gradually with the increase of plant oil content in base stock. The higher the proportion of plant oil in the base stock, the lower the enthalpy of IOs. All IOs were in the solid-like state, and the hardness of all IOs met the requirements of traditional plastic oil. All IOs were mainly in α and β′ crystal forms and can be used as basic stock for special oils. [ABSTRACT FROM AUTHOR]

This essay explores the uses of animal fat from domesticated livestock (cattle, swine, and sheep) in three separate, albeit closely related situations: as a substance harvestable from within the animal body, as a commodity reconfigured from its original form, and as a tool for scientific understanding in the late eighteenth and early nineteenth centuries. Using the concept of affordances, as initially described in the 1970s by James Jerome Gibson, and subsequentially amended by anthropologists, philosophers, and sociologists, as well as material culture or design historians, 1 trace animal fat across multiple stages of time and processing to show that while certain affordances remained constant throughout the period under consideration, material references to its origin within the animal body receded and ultimately disappeared. 1 explore the different forms of use and expectations that occurred in relation to animal fats within the cultural environments of the slaughterhouse, tallow chandlery, and soap-manufacturing facility. I conclude with the fundamental shift in ways of understanding animal fat that beginning in the late eighteenth century, transformed a substance once highly specific and linked directly to a particular animal's body into something that was subject to chemical analysis and ultimately synthetization. This paper is part of a special issue entitled "Making Animal Materials in Time," edited by Laurence Douny and Lisa Onaga. [ABSTRACT FROM AUTHOR]

The main purpose of this study was to investigate the effect of different levels of fiber and fat on performance, pH, and viscosity of digesta in young broilers. A total of 420 one-day-old male chicks Ross 308 were assigned to 60 cages of seven birds each. The birds fed with 15 experimental diets produced by central composite design (CCD), containing three levels of sugar beet pulp (SBP: 0.00, 1.75, 3.50%), tallow (T: 0.00, 0.50, 1.00%) and soybean oil (SO: 0.00, 0.50, 1.00%) from 0 to 14 d. The results showed that maximum average daily body weight gain (ADG: 42.10 g/b/d) and minimum feed conversion ratio (FCR: 0.99) were observed with diet containing 0.35% SBP, 0.00% T and 0.51% SO. Maximum (4.64) and minimum (3.01) pH in gizzard were found with diets containing 0.00% SBP, 0.51% T, 0.27% SO and 1.70% SBP, 0.21% T and 0.00% SO, respectively. The highest (6.94) and lowest (5.86) pH in ileum contents were obtained with diets comprising 0.00% SBP, 1.00% T, 0.43% SO and 1.47% SBP, 0.00% T, and 0.00% SO, respectively. Maximum (3.54) and minimum (1.50) viscosity in ileal digesta were observed with diets containing 3.50% SBP, 1.00% T, and 1.00% SO and 0.00% SBP, 0.00% T and 0.53% SO, respectively. This experiment has revealed that, the CCD and response surface methodology (RSM) have the efficiency to describe the relationships between different levels of SBP, T and SO and the ability to predict the optimal point of the level of diet ingredients in order to achieve the best performance. With increasing the amount of SBP and decreasing the level of T and SO in the diets, the pH of digesta in the gizzard and ileum decreased. The ileum digesta viscosity increased with increasing SBP, T, and SO levels. [ABSTRACT FROM AUTHOR]

Introduction: Fat inclusion in the diet presents positive features such as essential fatty acids and vitamin supply, slowing the passage rate, and lubricating the feed milling equipment. The price of conventional added fat sources has been increasing in the last few years; therefore, there is a growing interest in the use of alternative energy sources in poultry feeding. In this context, coproducts derived from the soybean oil refinement process represent an economic alternative and permit giving added value to residual products. Soybean lecithin, which is extracted from the soybean oil degumming process, is mainly composed of polar lipids (>60%), especially of phospholipids, but also contains an important amount of neutral lipids (30-40%), as triacylglycerols and free fatty acids. Soy lecithin may also act as a source of polyunsaturated fatty acids, which are important precursors of eicosanoids for human health benefits and neonatal growth. The inclusion of lecithin in the diet could improve lipid digestibility, liver function, and performance. Therefore, the present study was designed to evaluate the effect of fat source and soybean lecithin on performance, egg quality, nutrient digestibility, and blood parameters in laying hens. Materials and methods: A total of 144 Nick Chick laying hens were randomly divided into six treatments, six replicates, and four hens per replicate as a 2×3 factorial experiment in a completely randomized design. The experimental treatments included: 1) 3% soybean oil, 2) 3% soybean oil + 0.1% lecithin, 3) 3% tallow, 4) 3% tallow + 0.1% lecithin, 5) a mixture of soybean oil + tallow in equal proportions, and 6) a mixture of soybean oil + tallow in equal proportions + 0.1% lecithin. Lecithin contains 7480 kcal/kg metabolizable energy and is added to experimental diets at one kg/ton as per company recommendation. Productive performance and egg quality traits were recorded from 37 to 44 weeks of age. Egg production and egg weight were recorded daily and feed intake was recorded weekly. This information was used to calculate the feed conversion ratio. Egg quality was measured in four eggs which were individually weighed and the external and internal quality (shape index, yolk index, yolk weight, yolk color, Haugh unit, shell weight, and shell thickness) was determined. At the end of the experiment (44 weeks of age) celite was added to experimental diets as an insoluble marker. Apparent metabolizable energy, dry matter, fat and crude protein digestibility were measured. Liver enzymes' activity (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and lactate dehydrogenase concentrations) and blood parameters (triglyceride, cholesterol, HDL, and LDL) were measured at the end of the experiment. Results and discussion: Feed intake (P<0.01), egg production, and egg mass improved by a mixture of soybean oil + tallow (P<0.05) in the entire experiment. The effects of the source of oil on the productive performance of laying hens are contradictory and depend on the oil source and fatty acid compositions of oil/lipids, strain, and age of hens. Inclusion of lecithin in the diet increased feed intake and yolk color (P<0.01) but decreased Haugh unit (P<0.05). The reasons for the discrepancies among researchers concerning the effects of dietary lecithin on poultry production are not known but might depend on the type of bird used, as well as on the characteristics of the diet, including the level, source, and fatty acid profile of the lipid source used. The information available on the effects of lecithin on yolk pigmentation is scarce. The unsaturated fatty acid profile of lecithin is more favorable for xanthophyll absorption and utilization than that of the more saturated fatty acid profile of animal fat. Lecithin facilitates the absorption and transfer of fats, so it can cause the absorption of pigment substances as fatsoluble compounds. Yolk color increased and eggshell thickness was decreased (P<0.05) by a mixture of soybean oil + tallow. Apparent metabolizable energy, dry matter, fat, and protein digestibility were not affected by fat source, lecithin inclusion, or fat source × lecithin interaction. Moreover, the data indicated that the addition of soy oil decreased the cholesterol in the plasma (P<0.05). Aspartate aminotransferase (P<0.01), lactate dehydrogenase (P<0.05), triglyceride (P<0.01), cholesterol, and LDL (P<0.05) concentrations of the plasma decreased by lecithin inclusion in the diet. Lecithin is one of the natural elements that have dispersing properties. Lecithin is capable of reducing LDL cholesterol. It also promotes HDL cholesterol synthesis. In addition, it is used to help reduce cholesterol and triglycerides and protect the liver in the prevention of kidney stone formation. The lecithin modifies the cholesterol homeostasis in the liver, increasing the HMG-CoA reductase (3-hydroxy-3-methylglutaryl-coenzyme A reductase) and alpha 7 hydroxylase cholesterol activities. The LDL concentration and size are also significantly reduced and the bile acid pool and bile lipid secretion are increased. Conclusions: In general, soybean lecithin can be added, in combination with soybean oil and tallow as an energy source in laying hens' diet to improve performance. [ABSTRACT FROM AUTHOR]

Simple Summary: There have been reports on alternate feeding between diets based on different lipid sources, but how the intestinal microbiota of fish responds to alternate feeding strategies and its relevant roles in the host's health have not been reported. The present study provides novel results in this area. The main objective of the study was to assess how the intestinal microbiota of fish responds to alternate feeding between diets based on different lipid sources. Foremost, the juvenile turbot accepted alternate feeding between diets based on different lipid sources with no negative effects on survival or growth performance. Additionally, novel results were observed regarding the different bacterial compositions, microbial co-occurrence networks, and functional predictions among the different alternating feeding strategies. The results of this study indicate that a more comprehensive evaluation should be conducted from the perspective of intestinal microorganisms when a new feeding strategy is applied in aquaculture practices. These results will contribute to the nutritional regulation of aquatic animals from the perspective of microorganisms. A nine-week feeding trial was conducted to investigate changes in the intestinal microbiota of turbot in response to alternate feeding between terrestrially sourced oil (TSO)- and fish oil (FO)-based diets. The following three feeding strategies were designed: (1) continuous feeding with the FO-based diet (FO group); (2) weekly alternate feeding between soybean oil (SO)- and FO-based diets (SO/FO group); and (3) weekly alternate feeding between beef tallow (BT)- and FO-based diets (BT/FO group). An intestinal bacterial community analysis showed that alternate feeding reshaped the intestinal microbial composition. Higher species richness and diversity of the intestinal microbiota were observed in the alternate-feeding groups. A PCoA analysis showed that the samples clustered separately according to the feeding strategy, and among the three groups, the SO/FO group clustered relatively closer to the BT/FO group. The alternate feeding significantly decreased the abundance of Mycoplasma and selectively enriched specific microorganisms, including short-chain fatty acid (SCFA)-producing bacteria, digestive bacteria (Corynebacterium and Sphingomonas), and several potential pathogens (Desulfovibrio and Mycobacterium). Alternate feeding may maintain the intestinal microbiota balance by improving the connectivity of the ecological network and increasing the competitive interactions within the ecological network. The alternate feeding significantly upregulated the KEGG pathways of fatty acid and lipid metabolism, glycan biosynthesis, and amino acid metabolism in the intestinal microbiota. Meanwhile, the upregulation of the KEGG pathway of lipopolysaccharide biosynthesis indicates a potential risk for intestinal health. In conclusion, short-term alternate feeding between dietary lipid sources reshapes the intestinal microecology of the juvenile turbot, possibly resulting in both positive and negative effects. [ABSTRACT FROM AUTHOR]

为了开发消费者喜爱的牛油产品，对两种牛油（老火锅牛油与浓香牛油）的感官特征及其相关的风味物质进行了分析。采用适合项勾选法（CATA）探究两种牛油的感官特征，并分析了感官属性与喜好度之间的关系。通过固相微萃取-全二维气相色谱-质谱联用法（SPME-GC×GC-MS）测定两种牛油的挥发性风味物质含量，并采用气味活度值（OAV）确定关键风味物质。结果表明：浓香牛油的“肉香味”“焦香味”“干草味”和“蘑菇味”更突出，老火锅牛油的“脂香味”“膻味”和“腥味”更突出；老火锅牛油与浓香牛油中共检出80种挥发性风味物质，根据OAV分别鉴定出13、10种关键风味物质，这些关键风味物质呈现的香气特征与感官特征分析结果一致。开发牛油产品时，增加产品的“奶香味”“清新味”与“肉香味”，均可提高消费者喜好度。In order to develop the favorite beef tallow products of consumer, the sensory characteristics of different technological beef tallow （fragrant beef tallow and hot pot beef tallow） and the volatile compounds related to its sensory characteristics were analyzed, Check-all-that-apply was used to evaluate the sensory characteristics of the two beef tallow, and the relationship between sensory attributes and consumer preference was analyzed. Meanwhile, the contents of the volatile compounds of the two beef tallow were determined using solid phase micro-extraction-comprehensive two-dimensional gas chromatography-mass spectrometry,then the key flavor compounds were identified by odor activity value(OAV).The results showed that meat, fried, hay and mushroom were more prominent in fragrant beef tallow, while fat, cowy and raw were more prominent in hot pot beef tallow. A total of 80 volatile compounds were detected in hot pot beef tallow and fragrant beef tallow, and 13 and 10 key volatile compounds were identified according to OAV, respectively. And the aroma profiles presented by key volatile compounds were consistent with the results of sensory characteristics. When developing beef tallow products, increasing the creamy, freshness and meat flavor of the products can improve consumer preference.

ABSTRACT The present research aims to evaluate the inclusion of an emulsifying additive based on mono- and diglycerides of fatty acids in diets with different lipid sources, studying its effects on duodenum histology, biometry of the digestive and reproductive tract, and blood biochemical profile of Japanese quails. The study was carried out in the experimental aviary of the Federal University of Grande Dourados, Mato Grosso do Sul, Brazil. The experimental design was completely randomized, with diets in a 3x2 factorial design with three different lipid sources (soybean oil, poultry fat, and beef tallow), either supplemented or not with an emulsifier. The birds that received a diet composed of poultry fat and an emulsifying additive had a larger gizzard. Animals that received a diet containing an emulsifier, regardless of the lipid source, had greater heart weight. The duodenum of animals that received bovine tallow in their diet showed a greater height and villus:crypt ratio. Biochemical parameters were not affected by either the lipid source or the inclusion of emulsifier in quail diets. The biochemical blood profile of birds in treatments did not show significant changes among all diets used. The use of soybean oil, bovine tallow, and poultry fat with or without the inclusion of an emulsifier does not show changes in the health and development of the quails’ organs, which also indicates that the health of Japanese quails in the laying phase is kept in good standards.

The purpose of the study was to examine the impact of adding Fennel seeds (Foeniculum vulgare L.) into the diets of laying hens on egg quality. This study was conducted in poultry fields belonging to Department of Animal Production, College of Agriculture, and University of Anbar for 12 weeks. A total of 72 laying hens (Lohman Brown) were used in this experiment, at age 43 weeks. Hens were randomly distributed to six treatments groups and with four replicates per treatment (3 hens/replicate). The treatments were T1: (positive control) based diet containing 1% hydrogenated vegetable fat, T2: (negative control) based diet containing 1% Sheep fat, T3, T4, T5 and T6 The based diet contained 1% Sheep fat + supplementation of 0.25, 0.50, 0.75 and 1% Fennel seeds powder sequentially. The results showed significant (P≤0.05) differences for the supplementation treatments on the qualitative characteristics of egg quality, as treatment T6 was significantly (P≤0.05) superior to the rest of the experimental treatments in shell weight, yolk weight, yolk index, percentage of Albumen weight %, Albumen weight (g) and Haugh Unit. Treatments T5 and T6 were significantly (P≤0.05) superior to the rest of the experimental treatments in shell thickness, percentage of yolk weight%, and Albumen index, while there was no significant effect (P≤0.05) of the supplementation treatments on (percentage of shell weight%) compared to the two control treatments T1 and T2. It was concluded adding 0.75 and 1% Fennel seeds powder to laying hens' diets improved quality of eggshell, yolk, and egg white.

为保障牛油的食用安全和风味品质，以被邻苯二甲酸二正丁酯（DBP）和邻苯二甲酸二（2-乙基）己酯（DEHP）污染的生牛脂为原料，对其进行炼制，考察炼制条件对牛油中DBP和DEHP含量的影响，采用电子鼻分析炼制条件对牛油风味的影响。结果表明：牛油中DBP、DEHP含量随炼制温度、炼制时间、炼制压力和加水量的变化表现出不同的变化规律，适宜炼制条件为炼制温度120~140 ℃、炼制时间10~15 min、炼制压力-0.02 MPa、加水量2 mL/100 g，在此条件下炼制的牛油DBP和DEHP含量较低且稳定；氮氧化合物、硫化物、萜类化合物、短链烷类、醇醚醛酮类化合物是牛油的主要风味成分。通过线性判别分析可知，加水量、炼制压力、炼制时间对牛油风味影响较大。 In order to ensure the edible safety and flavor quality of tallow,the refining of raw beef fat contaminated with di-n-butyl phthalate (DBP) and di(2-ethyl)hexyl phthalate (DEHP) was carried out to investigate the effects of refining conditions on the contents of DBP and DEHP in tallow, and the effects of refining conditions on the flavor of tallow were analyzed by electronic nose. The results showed that the contents of DBP and DEHP in tallow changed differently with the changes of refining temperature, refining time, refining pressure and water-adding amount. The suitable refining conditions were refining temperature 120-140 ℃, refining time 10-15 min, refining pressure -0.02 MPa, water-adding amount 2 mL/100 g. Under these conditions,the contents of DBP and DEHP were low and stable. Nitrogen oxides, sulfides, terpenoids, short chain alkanes, alcohol, ethers, aldehydes and ketones were the main flavor components of tallow. Through linear discriminant analysis, it could be seen that the water-adding amount, refining pressure and refining time had a greater impact on the flavor quality of tallow.

Objective To investigate the physiological effects of exogenous emulsifiers in broiler chickens that were fed tallow-incorporated reduced-energy diets over 35 days. Methods A total of 256 Ross 308 one-day-old broilers (42.28±0.16 g) were randomly allocated in a 2×2 factorial arrangement to 32 pens with eight chicks per cage. Birds were fed one of four dietary treatments as follows: i) positive control (PCN; energy sufficient diet); ii) negative control (NCN; energy-deficient diet, −100 ME kcal/kg); iii) PCL (PCN plus 0.05% emulsifier); and iv) NCL (NCN plus 0.05% emulsifier). Growth performance was evaluated weekly whereas assessments for the carcass traits, digestibility, some blood metabolites, ileal morphology, and meat quality were measured on d 21 and d 35. Results Birds fed the NCL diet had higher (p

Simple Summary: Despite the US Food and Drug Administration's zero-tolerance policy against Salmonella, several Salmonella-linked outbreaks and recalls linked to pet foods have been reported. Post-processing steps, such as fat and flavor coating, drying, cooling, and packaging are common steps where Salmonella becomes contaminated in dry pet food kibbles. Rendered animal fats and oils are commonly coated on kibbles to enhance palatability and increase energy density. A tiny layer of water in the bottom of bulk fat transport trucks or/and storage tanks could easily harbor Salmonella, leading to its entry into the pet food during coating. In this study, different types of acidulants were applied in the fat and oil system, and their effect against Salmonella in the water or fat phase in different types of fats and oils was evaluated. Our results were promising, wherein all the tested acidulants were effective to mitigate Salmonella from the fat or oil system (both aqueous and fat phase) within 2 h. The findings of this study could be helpful to the rendering and pet food industry in a fight to mitigate Salmonella in pet food. Salmonella-contaminated pet foods could potentially become a source of human salmonellosis. This study evaluated the survival of Salmonella without and with the addition of acidulants in different fat types (chicken fat (CF), canola oil (CO), Menhaden fish oil (FO), lard (La), and tallow (Ta)) commonly used to coat dry pet food kibbles. The minimum inhibitory concentration (MIC) of individual acidulants and the combination were determined using the broth microdilution method. Autoclave-sterilized rendered fats were treated with pre-determined concentrations of antimicrobial acidulants (0.5% sodium bisulfate (SBS), 0.5% phosphoric acid (PA), 0.25% lactic acid (LA), etc.) and incubated overnight at 45 °C. The treated fats were inoculated with approximately eight logs of a Salmonella cocktail. Microbiological analyses were conducted separately for the fat-phase and water-phase at predetermined time intervals (0, 2, 6, 12, and 24 h) by plating them onto TSA plates. After incubating at 37 °C for 24 h, the plate count results were expressed as log CFU/mL. The MIC of SBS was 0.3125%, and of PA and LA were both 0.1953% against cocktail Salmonella serotypes. We observed a possible synergistic effect when SBS and organic acid were combined. All the acidulant tested at targeted concentrations individually as well as in combination with organic acids were highly effective against Salmonella spp. (non-detectable within 2 h) across different fat types. A potent anti-bactericidal effect leading to non-detectable Salmonella immediately (<1 h) at 45 °C was observed in the aqueous phase of the fish oil system, even without the addition of acidulants. These findings are significant for the dry pet food industries, where potential post-processing contamination of Salmonella could be controlled by treating fats and oils with acidulants. [ABSTRACT FROM AUTHOR]

The effect of temperature was studied on the synthesis of fatty acid alkyl esters by means of transesterification of waste beef tallow using ethanol and, iso-butanol and 1-butanol at supercritical conditions. These alcohols are proposed for the synthesis of biodiesel in order to improve the cold flow properties of alkyl esters. Alcohol–beef tallow mixtures were fed to a high-pressure high-temperature autoclave at a constant molar ratio of 45:1. Reactions were carried out in the ranges of 310–390 °C and 310–420 °C for ethanol and iso-butanol, respectively; meanwhile, synthesis using 1-butanol was assessed only at 360 °C. After separation of fatty acid alkyl esters, these samples were characterized by nuclear magnetic resonance (NMR) and gas chromatography coupled to mass spectrometry (GC-MS) to quantify yields, chemical composition, and molecular weight. Results indicated that yields enhanced as temperature increased; the maximum yields for fatty acid ethyl esters (FAEEs) were attained at 360 °C, and for fatty acid butyl esters (FABEs) were achieved at 375 °C; beyond these conditions, the alkyl ester yields reached equilibrium. Concerning the physicochemical properties of biodiesel, the predicted cetane number and cloud point were enhanced compared to those of fatty acid methyl esters. [ABSTRACT FROM AUTHOR]

This study aimed to develop nutritious and healthy Sichuan hotpot oil. Four blended oil formulas were formulated using MATLAB based on the fatty acid composition of four base constituents (beef tallow, mutton tallow, peanut oil, and palm oil). The sensory characteristics, physicochemical properties, nutritional composition, harmful substances, and antioxidant capacity of the oils were analyzed during the boiling process. A blend of 60% beef tallow + 10% mutton tallow + 10% peanut oil + 20% palm oil exhibited a low level of peroxide (0.03 g/100 g) and malondialdehyde (0.04 mg/kg), and high phytosterol content (1028.33 mg/kg), which was the suitable hotpot blending oil. Furthermore, the changes in the physicochemical properties during boring were low, with a high retention rate of phytosterol (94.85%), and the levels of 3,4-benzopyrene (1.12 μg/kg) and 3-monochloropropane-1,2-diol ester (0.67 mg/kg) were both lower than the recommended limits. This study will provide a theoretical basis for the advancement of the hotpot oil industry. [ABSTRACT FROM AUTHOR]

MgO nanoparticles (MgONPs) have been successfully synthesized using ZOE (Zingiber officinale Roscoe extract in water) and applied for catalytic conversion of beef tallow to bio gasoline, kerosene and diesel. ZOE was used due to containing the alkaloid as a weak base source to hydrolyze Mg(NO3)2 precursor and form the MgONPs. The synthesized MgONPs was characterized using UV-Vis spectrophotometer, X-ray diffraction (XRD), particle size analyzer (PSA), Brunauer?Emmett?Teller (BET) surface areas, UV–Vis diffuse reflectance spectrophotometry (DRS), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) and transmission electron microscopy (TEM). The catalyst activity was performed to convert beef tallow to biofuel in the stainless-steel reactors at 3000C for 60 min. The conversion results of beef tallow were analyzed using gas chromatography-mass spectrometry (GC-MS). The beef tallow conversion shows that all fatty acids derived from beef tallow are converted to gases and liquids fractions. The conversion using catalyst to feed weight ratio of 4 wt. % produced the liquid fractions containing the dominant of alkanes (62.85%) and cyclic compounds (18.77%). Pentadecane and Heptadecane are the main compounds in liquid products, which indicates a decarboxylation reaction.

The present study explores biodiesel production from the single-cell oil (SCO) synthesized by Yarrowia lipolytica MTCC 9520, utilizing slaughterhouse lipid waste, goat tallow, as the carbon substrate. Various parameters, including cultivation time (96 h), pH(6), substrate concentration (1.5%, v/v), inoculum size (5%, v/v), and C/N ratio (100), were optimized to attain the maximum biomass and lipid yield and lipid content of 3.8 g/L, 2.6 g/L, and 69.3% (g/g dry weight), respectively. The presence of intracellular lipid bodies in Y. lipolytica was confirmed by observing the Nile red-stained cells by fluorescence microscopy. Besides, the fluorescence intensities of the intracellular lipid bodies were determined by the flow cytometer. The extracted SCO from Y. lipolytica MTCC 9520 was analyzed by Fourier transform infrared (FT-IR) spectroscopy. Gas chromatography-mass spectrometry (GC-MS) analysis of the transesterified SCO presented the fatty acid methyl ester (FAME) profile, including palmitic acid (42.9%), stearic acid (21.5%), myristic acid (18.3%), and oleic acid (7.0%). The obtained FAME composition was further used to predict the properties of the biodiesel. The revealed characteristics of the transesterified FAMEs signify the candidature of the synthesized SCO as an alternate feedstock for biodiesel production. [ABSTRACT FROM AUTHOR]

The production of preparations based on natural ingredients has become a priority in the cosmetic and pharmaceutical industries. The novelty of the study was to present a possible method for obtaining pure diacylglycerols (DAG) from natural raw materials like fats (hemp seed oil and mutton tallow). We have determined the optimum water content for enzymatic interesterification to shift the reaction equilibrium towards hydrolysis and obtain the highest DAG formation yield. Then, DAG were separated and purified by column chromatography. Raw fats were characterized by fatty acids composition and their positional distribution using gas chromatography. Triacylglycerols, diacylglycerols, monoacylglycerols, and free fatty acid contents were determined using gel permeation chromatography. The main fatty acid found in sn-2 MAG of hemp seed oil was linoleic acid, which was followed by oleic acid. Saturated fatty acids in hemp seed oil's TAG showed a preference for the outer sn-1,3 positions. For mutton tallow, the majority of oleic acid was found at sn-2 position. The optimum water content applied during enzymatic interesterification for DAG formation was estimated to be 2.0% w/w, using the following conditions: 5% w/w of catalyst, 200 rpm, 60°C, 6 h. DAG yield was 27%. Purification of the mutton tallow-based and hemp seed oil-based DAG yielded 83% w/w and 82% w/w, respectively. High-purity DAG could be applied as effective emulsifiers in food, cosmetic or pharmaceutical emulsions. [ABSTRACT FROM AUTHOR]

Tallow is a less economical and underutilized animal's fat. In fact, tallow comprises triglycerides from several fatty acids which support skin protection by increasing moisture and preventing dryness. The use of tallow in soap manufacturing will gain its interest and enhance its value added. In this study, solid soaps are prepared using tallow as raw materials by adding kefir curd from goat milk. This study analyzed the physicochemical and organoleptic characteristics of the particular soaps by developing a completely randomized design (CRD) experiment. The kefir curd gradually increased its proportion in soap manufacturing and observed its effect on pH, moisture content, degree of foaming, and foam stability. Organoleptic tests for both hedonics and hedonic qualities were also performed and analyzed by Kruskal Wallis's nonparametric analysis. The statistical analysis reported a significant difference of 6% curd addition on pH (p0.05).

IntroductionMetabolic syndrome (MetS) is considered as a complex, intertwined multiple risk factors that directly increase the risk of various metabolic diseases, especially cardiovascular atherosclerotic diseases and diabetes mellitus type 2. While lifestyle changes, including dietary intervention are effective in mitigating or preventing MetS, there are no specific therapies against MetS. Typical western diets comprise of high saturated fatty acid, cholesterol, and simple sugar; consequently their consumption may increase the potential pathological developmental risk of MetS. Partial replacement of dietary fatty acids with polyunsaturated fatty acids (PUFAs) is widely recommended measure to manage MetS-related disorders.MethodsIn the present study, we used rat model to investigate the role of n-3 PUFA enriched beef tallows (BT) on MetS and tunicamycin (TM)-induced endoplasmic reticulum (ER) stress, by partially replacing dietary fat (lard) with equal amounts of two different BTs; regular BT or n-3 PUFA-enriched BT. The experimental rats were randomly assigned to three different dietary groups (n = 16 per group): (1) high-fat and high-cholesterol diet (HFCD); (2) HFCD partially replaced with regular BT (HFCD + BT1); (3) HFCD partially replaced with n-3 enhanced BT (w/w) (HFCD + BT2). After 10 weeks of dietary intervention, each experimental rodent was intraperitoneally injected with either phosphate-buffered saline or 1 mg/kg body weight of TM.ResultsHFCD + BT2 showed improved dyslipidemia before TM injection, and increased serum high-density lipoprotein cholesterol (HDL-C) levels after TM injection. BT replacement groups had significantly reduced hepatic triglyceride (TG) levels, and decreased total cholesterol (TC) and TG levels in epididymal adipose tissue (EAT). Furthermore, BT replacement remarkably attenuated TM-induced unfolded protein responses (UPRs) in liver, showing reduced ER stress, with BT2 being more effective in the EAT.DiscussionTherefore, our findings suggest that partially replacing dietary fats with n-3 PUFA to lower the ratio of n-6/n-3 PUFAs is beneficial in preventing pathological features of MetS by alleviating HFCD- and/or TM-induced dyslipidemia and ER stress.

Objective: To investigate the physiological effects of exogenous emulsifiers in broiler chickens that were fed tallow-incorporated reduced-energy diets over 35 days. Methods: A total of 256 Ross 308 one-day-old broilers (42.28±0.16 g) were randomly allocated in a 2×2 factorial arrangement to 32 pens with eight chicks per cage. Birds were fed one of four dietary treatments as follows: i) positive control (PCN; energy sufficient diet); ii) negative control (NCN; energy-deficient diet, -100 ME kcal/kg); iii) PCL (PCN plus 0.05% emulsifier); and iv) NCL (NCN plus 0.05% emulsifier). Growth performance was evaluated weekly whereas assessments for the carcass traits, digestibility, some blood metabolites, ileal morphology, and meat quality were measured on d 21 and d 35. Results: Birds fed the NCL diet had higher (p<0.05) body weights, daily gains, daily feed intake, and improved feed efficiency over the entire 35-day period. Improvements (p<0.05) for the ileal digestibility of crude fat, energy, and dry matter commensurate with longer (p<0.05) villus heights were also observed with emulsifiers in the NCL and PCL diets. For the carcass measurements, only the liver weights were increased (p<0.05) with emulsifiers in the supplemented groups. For blood metabolites, higher (p<0.05) lipase levels were noticed with emulsifiers in the NCL and PCL diets. In addition, marginal reductions (p = 0.076; p = 0.095, respectively) were also noted with emulsifiers for the total cholesterol and triglyceride contents on d 35. Regarding meat quality, breast muscle yellowness was increased (p<0.05) with emulsifier use in supplemented groups. Conclusion: Our results suggest that emulsifier supplementation at 0.05% in diets could potentially improve the growth performance and nutrient digestibility of broilers over 35 days. This could compensate for the lower growth performance that could be recorded with fat-incorporated lower-energy diets. [ABSTRACT FROM AUTHOR]

Introduction1 Feed cost has gradually increased in poultry production worldwide. One approach to minimizing production cost is dietary manipulation of nutrient supplies through improved feed efficiency. The inclusion of lipids in broiler diets is essential to meet metabolisable energy requirement. Great amounts of animal fats and vegetable oils are usually added to broiler diets to increase their energy content. However, several factors can affect lipids digestion, both related to the animal characteristics such as bird age, genetic strain, secretion and activity of digestive enzymes, and to the diet composition such as type of fat used as lipid supplement, ratio of unsaturated to saturated fatty acids in the diet, presence of pentosans and dietary fiber. Within this reality, researches have been conducted to study the increased digestibility of oils and fats by emulsifying substances, such as bile salts and phospholipids. Lysophospholipid (LPL) is a more effective biosurfactant in emulsifying properties than bile salts and soy lecithin because one molecule in the hydrophobic tail is removed, indicating more stability in the aqueous environment of the gastrointestinal tract. Another action of lysophospholipids is related to the antibacterial effect. They may modify the biological structure of bacteria causing changes in membrane permeability, raising the speculation of a combined effect with organic acids on poultry gut health. The changes in membrane permeability by lysophospholipids may facilitate the ion dissociation of the organic acids inside the bacteria. In turn, the control of the intestinal microbiota through these additives could preserve the integrity of endogenous, consequently, the present study was designed to investigate the effects of dietary LPL supplementation in diets differing in fat on blood parameters, intestinal morphology, ceca microbiome and performance in broiler chicks. Materials and Methods This experiment was performed in a completely randomized design based on factorial arrangement to evaluate the effect of lysophospholipid supplementation (0 and 0.25g/kg) in cornsoybean meal diets containing three fat sources (soybean oil, tallow and their blend 50: 50%) and each at two levels (1.5 and 3%) with 12 isocaleric and isonitrogenous starter diets. Each diet was fed to six replicates of 10 chicks each during 1 to 14 days of age. Performance traits, jejunal morphology, serum lipid composition, cecal microbial population of one chick from each replicate were measured at day 14. Results and Discussion the interaction effects of lysophospholipid supplementation, fat source and fat level were not significant on broiler performance during 1 to 14 days of age, except for the lysophospholipid supplementation and fat level that was significant on feed intake in the starter period. Among the main effects only fat levels had a significant effect on body weight, body weight gain in the starter period, and 3% fat level compared to 1.5% improved body weight and body weight gain. These results are in agreement with zampiga et al, (2016) that demonstrated the addition of the lysophospholipid did not statistically improve final body weight and daily weight gain of broilers in the whole period of trial (0-42 d). These observations are partially in contrast with Melegy et al. (2010) who reported that the use of an emulsifier based on lysolecithin at the dosage of 0.25 or 0.5 kg/ton of feed significantly improved these productive parameters. Other factors may be affect on broiler performance such as inappropriate dose of lysophospholipid in diet, low energy levels of the experimental diets, use of 3 factors in the experimental design, and low bird population in each treatment that combined the effects. The Interaction effects of lysophospholipid supplementation, fat source and fat level were not significant on jejunal villus height, villus width, diameter of epithelium layer, crypt depth and villus height to crypt depth ratio. The interaction effects of lysophospholipid supplementation and fat levels in diet showed a significant effect on villus width. The effect of fat levels was significant for villus height and it increased by the use of 3% levels. The Interaction effect of lysophospholipid supplementation, fat source and fat level was not significant on the concentration of triglyceride, cholesterol, HDL and LDL in serum of broiler chicks. The interaction effect of lysophospholipid supplementation and fat level in diet was significant on the concentration of triglyceride level, and the interaction effect of lysophospholipid supplementation and fat source in diet was significant on LDL in serum lipid level of broiler chicks. The main effect of lysophospholipid supplementation was significant on the concentration of triglyceride, and also the effect of fat source was significant on cholesterol and HDL in serum of broiler chicks. The Interaction effects of lysophospholipid supplementation, fat source and fat level was not significant for lactobacillus and E-coli population in ceca. The main effect of lysophospholipid supplementation was significant increase on the lactobacillus population in ceca, and the effect of fat level in diet was significant decrease on the E-coli population in ceca. Conclusion It can be concluded in this study that of lysophospholipids supplementation did not have a significant effect on performance in broiler chickens during the starter period. [ABSTRACT FROM AUTHOR]

Introduction1 To date, there is not any accurate estimation of calf mortality in the world; however, annual pre-weaning calves' mortality was estimated to be around 7.8, 6.5, 5.5, and 2.6% in the United States, Iran, China, and Sweden, respectively. Raboisson et al. (2013) represented that most neonatal calf mortality happens at age under one month. Hill et al. (2011) reported that nutritional factors could modulate the calf immune system's functions. Studies on non-ruminants confirm that the consumption of polyunsaturated fatty acids (PUFA) relating to the n-3 FA can affect the immune response. In calves' nutrition, using PUFA in milk or milk replacer (MR) had a pleasant effect on immune responses and antioxidant status. Supplementation n-3 FA, especially EPA and DHA, would increase the proportion of PUFA in the membrane phospholipids, which might change the performance of the immune system. The n-3 PUFA plays a critical role in influencing the immune system through various mechanisms described in detail by Calder (2012). Previous studies showed that adding n-3 PUFA to milk or MR decreases the symptoms of diarrhea and inflammatory diseases caused by viral or bacterial infections. So far, there are not enough reports regarding dietary n-3 PUFA on the APR in neonatal calves. Nevertheless, most research regarding FO supplementation and its anti-inflammatory effects on neonatal calves' health has been done on long-term consumption. As earlier mentioned, most calf mortality occurs at the first 30 days of age; consequently, long-term (more than one month) consumption of FO might not provide clear evidence to evaluate the anti-inflammatory effect of FO on the status of neonatal calves' health. Therefore, the purpose of this study was the first evaluation of short-term supplementation of n-3 PUFA on the APR of neonatal calves. Materials and Methods Twenty-four bull calves, with a mean age of 34.5 3.7 days, were housed outdoors in individual pens bedded with wheat straw at the dairy farm facilities of Astan Quds Razavi Animal Husbandry and Agriculture Co. (Mashhad, Iran) in February 2019. The criteria for calf selection were, namely, the type of calf delivery (without any difficulty) and no history of disease or diarrhea. To achieve a quantitative similarity between calves, we used age and body weight as further criteria. The experiment's duration was 11 days (a week before LPS challenge and three days after LPS challenge) with an adaptation period (seven days). After the adaptation period, calves were weighed (57.5 ± 4.4 kg) and randomly assigned to 1 of 4 groups (six calves/group). Randomized calves received treatments during the study period according to the group they were already allocated: 1. negative control group (NC), 2. Positive control group (LPS challenge, PC), 3. Tallow 350 mg/kg BW group + LPS (TA), 4. Fish oil 350 mg/kg BW group + LPS (FO). All calves were fed the same diet, 5 L/d of whole milk, and had free access to freshwater during the experiment. The PC, FO, and TA groups were intravenously challenged with 0.5 μg/kg BW ultrapure LPS from E. coli serotype O111:B4 (Sigma-Aldrich: registered; product NO. L2630) on day eight. Treatments FO and TA were mixed with whole milk and were offered two times a day (at 0800 and 1700). FO and TA groups were isocaloric to compare the effect of manipulating fatty acid intake in the same level of energy intake on the APR of neonatal calves. The blood samples were collected at 1, 2, 3, 4, 6, 12, 24, 48, 72 h, post LPS challenge (p.c.) to evaluate inflammatory condition. The clinical signs (RT, RR, and HR) were recorded at 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, 8, 12, 18, 24 h p.c. According to Plessers et al.'s (2015) model, the appearance of behavioral phases (respiratory, depression, and recovery phase) was assessed. Data were analyzed as a completely randomized design by using JMP (13.2) software. Results and Discussion The results of this study confirm previous experiments that showed a significant increase of cytokines level by the LPS administration (26, 29). As expected, the IL-6 increased when the TNF-α decreased (Maximal level at 3 and 1 h p.c., respectively). There was no significant difference in cytokines and APPs between PC, FO, and TA, while the FO had the minimum level. The typical sickness behavior of LPSchallenged calves was distinguished as respiratory, depression, and recovery phases according to Plessers et al.'s (2015) model. In this study, there was no significant effect of decreasing n-6/n-3 FA ratio on sickness behavior. Besides, the level of inflammatory cytokines and acute-phase proteins were not affected by experimental groups. These results were in line with McDonnell et al., (2019) reported no FO effect on immune function during the pre-weaning period. Although the level of DHA + EPA requirement for calves has not been well known, studies represented that their highest level in humans is 5 g/d. Stanley et al. (2007) concluded that the n-6/n-3 FA ratio might not be a helpful concept and distracts attention from increasing absolute intakes of long-chain n-3 FA. In this regard, Flaga et al. (2019) represented that DHA-rich algae supplementation in milk replacer could decrease cytokines' mRNA expression. They suggested that 3 g/d DHA might be the maximum level in neonatal calves' diet with an appropriate effect on the immune system. In the current study, NC, PC, and TA received 2 mg/d, and FO received 3 g/d DHA. It might be worthwhile considering the amount of DHA + EPA when FO is used as an n-3 PUFA source in calves' diet. Conclusion The results showed that decreasing the n6/n3 FA ratio in diets by supplementing FO could not affect acute phase response in calves. Besides, short-term supplementation of FO could not improve calves' immune systems as no differences in cytokines and APP between PC and FO were observed. Although sickness behavior in FO finished sooner than PC, there was no significant difference between them. In this study, increasing MUFA intake could not affect APR in calve. It seems that more studies are needed to evaluate the effect of EPA and DHA on the performance and health status of calves. [ABSTRACT FROM AUTHOR]