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PROFİLE OF URİTİCA DİOİCA L. SEED EXTRACT ON OXİDATİVE DNA DAMAGE İN LİVER TİSSUE AND WHOLE BLOOD OF RADİATİON INDUCED RATSTahir ÇAKIR1, Kenan YILDIZHAN1, Zübeyir HUYUT2, Ahmet UYAR31 Yuzuncu Yil University, Faculty of Medicine, Department of Biophysics, Van, Turkey2 Yuzuncu Yil University, Faculty of Medicine, Department of Biochemistry, Van, Turkey3 Yuzuncu Yil University, Faculty of Veterinary, Deparment of Pathology, Van, TurkeyAbstract Aim: Ionizing radiation is commonly used to treatment options in the majority of cancer patients, but radiotherapy applications leads to oxidative DNA damage in non-targeted tissues. In this study, we investigated the radioprotective effects of Uritica dioica L. seed extract (UDSE) against oxidative DNA damage in whole blood and liver tissues of irradiated rats.Methods: 32 rats (eight weeks) divided into 4 group (n:8). Control group (C); was fed with pellet for 10 days. Radiation group (IR) was fed with pellets for 10 days after exposing 5Gy radiations as a single fraction. Radiation+UDSE (IR+UDSE) group was exposed 5Gy radiations as a single fraction and was fed with UDSE for 10 days. UDSE group (UDSE) was fed with only UDSE for 10 days. The rats were sacrificed under anesthesia (50mg/kg ketamine). 8-hydroxy-2-deoxyguanosine (8-OHdG) and dG levels were measured by high pressure liquid chromatography(HPLC) method in liver tissue and whole blood. Histopathological changes in the liver tissues were determined by immunohistochemistry.Results: 8-OHdG/106dG levels of liver tissues and whole blood were increased and degenarations were observed in liver tissues in IR group (p?0.05). However, 8-OHdG/106dG levels of liver tissue and whole blood, and degenarative changes of liver tissues were decreased in the IR+UDSE group compared to the IR group (p?0.05). 8-OHdG/106dG levels also were lower in whole bloods of UDSE group, and we did not observe any degeneration in liver tissues of this group.Conclusions: It was observed that radiation increased oxidative DNA damage and caused to degenerative changes in liver. But, after exposure to radiation, the administration of UDSE that has antioxidant properties may reduce oxidative DNA damage and degenerative changes revealed by the effects of radiotherapy.Key Words: Antioxidant, Urtica dioica, ionizing radiation, oxidative DNA damage, oxidative stress.

In this study, gamma shielding properties of concretes containing magnetite and limonite ores at different ratios (5%, 10%, 15%, 20%, and 30%) were investigated by using a Co-60 (1.25 MeV) radioactive source which was built in Thratron 1000E (TM) device used for radiotherapy purposes. Then, the photon transmission values (I/I-0) were measured by 0.6 cm(3), farmer type, PTW (TM) ion chamber. The dose readings (I-0 and I), made by PTW (TM) Unidose Electrometer, were used to calculate the linear attenuation coefficient (mu, cm(-1)), the mean free path (lambda, cm), and the mass attenuation coefficient (mu(rho), cm(2)/g) by the Lambert equations, and the results were compared with the calculations obtained using the WinXCom computer program. The findings from this study revealed that the concretes containing magnetite and limonite ores were more effective than the ordinary concrete in the attenuation of gamma rays. It was determined that the values of the mean free path decreased with the increasing concentrations of magnetite and limonite ores while the linear attenuation coefficients for 10 concrete blocks increased with the increasing magnetite and limonite ore concentrations. A comparison of the theoretical values obtained from the WinXCom computer program and the experimental results revealed a good level of conformity.

The aim of study was to evaluate the effects ontarget volume (brain) and lens doses with different treatment plannings in patients who received whole brain radiotherapy because of brain metastases. The system tomography scans of 14 patients at 0.5 cm intervals, were transferred to three-dimensional treatment planning computer and the normal tissues and target volumes were outlined. Seven different treatment plannings were done for each patient. Plan I: Angled blocked plan; Plan II: Non-angled blocked plan; Plan III: Helmet plan; Plan IV: Blocked plan with the inferior border at the inferior orbital ridge; Plan V: Angled unblocked plan; Plan VI: Non-angled unblocked plan; Plan VII: Conventially planned non-angled unblocked plan. Brain and lens minimum, maximum and mean doses and brain V95 and D95 doses were recorded for each plan using dose-volume histograms.