1. Insights into physiological traits of Bifidobacterium animalis subsp. lactis BB-12 through membrane proteome analysis
- Author
-
Gilad, Ofir, Hjernø, Karin, Østerlund, Eva C., Margolles, Abelardo, Svensson, Birte, Stuer-Lauridsen, Birgitte, Møller, Anders L. Blom, and Jacobsen, Susanne
- Subjects
- *
BIFIDOBACTERIUM , *PROTEOMICS , *PROBIOTICS , *MEMBRANE proteins , *OLIGOSACCHARIDES , *ENDOPEPTIDASES , *CHROMOSOMAL translocation , *ADENOSINE triphosphatase - Abstract
Abstract: Bifidobacterium animalis subsp. lactis BB-12 is a widely used probiotic strain associated with a variety of health-promoting traits. There is, however, only limited knowledge available regarding the membrane proteome and the proteins involved in oligosaccharide transport in BB-12. We applied two enrichment strategies to improve the identification of membrane proteins from BB-12 cultures grown on glucose and on xylo-oligosaccharides, the latter being an emerging prebiotic substrate recently reported to be fermented by BB-12. Our approach encompassed consecutive steps of detergent- and carbonate-treatment in order to generate inside-out membrane vesicles and to interfere with binding of membrane-associated proteins to the membrane, respectively. Proteins in the enriched membrane fraction and membrane-associated fraction were digested by lysyl endopeptidase and trypsin followed by peptide sequencing by LC-ESI-Q-TOF MS/MS. Ninety of a total of 248 identified unique proteins were predicted to possess transmembrane segments (TMSs), and 56 of these have more than one TMS. Seventy-nine of the identified proteins are annotated to be involved in transport of amino acids, oligosaccharides, inorganic ions, nucleotides, phosphate or exopolysaccharides, or to belong to the F1F0-ATP-synthetase complex and the protein translocation machinery, respectively. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF