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Your search keyword '"ŠATKAUSKAS, Saulius"' showing total 273 results
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273 results on '"ŠATKAUSKAS, Saulius"'

9. The Role of Mutated Calreticulin in the Pathogenesis of BCR-ABL1 -Negative Myeloproliferative Neoplasms.

Author

Vadeikienė, Roberta, Jakštys, Baltramiejus, Laukaitienė, Danguolė, Šatkauskas, Saulius, Juozaitytė, Elona, and Ugenskienė, Rasa

Subjects
MYELOPROLIFERATIVE neoplasms, CALRETICULIN, OXIDATIVE stress, CELL cycle, BONE marrow
Abstract

Myeloproliferative neoplasms (MPNs) are characterized by increased proliferation of myeloid lineages in the bone marrow. Calreticulin (CALR) 52 bp deletion and CALR 5 bp insertion have been identified in essential thrombocythemia (ET) and primary myelofibrosis (PMF). There is not much data on the crosstalk between mutated CALR and MPN-related signaling pathways, such as JAK/STAT, PI3K/Akt/mTOR, and Hedgehog. Calreticulin, a multifunctional protein, takes part in many cellular processes. Nevertheless, there is little data on how mutated CALR affects the oxidative stress response and oxidative stress-induced DNA damage, apoptosis, and cell cycle progression. We aimed to investigate the role of the CALR 52 bp deletion and 5 bp insertion in the pathogenesis of MPN, including signaling pathway activation and functional analysis in CALR-mutated cells. Our data indicate that the JAK/STAT and PI3K/Akt/mTOR pathways are activated in CALR-mutated cells, and this activation does not necessarily depend on the CALR and MPL interaction. Moreover, it was found that CALR mutations impair calreticulin function, leading to reduced responses to oxidative stress and DNA damage. It was revealed that the accumulation of G2/M-CALR-mutated cells indicates that oxidative stress-induced DNA damage is difficult to repair. Taken together, this study contributes to a deeper understanding of the specific molecular mechanisms underlying CALR-mutated MPNs. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Physical Methods for Drug and Gene Delivery Through the Cell Plasma Membrane

Author

Jakutavičiūtė, Milda, Ruzgys, Paulius, Tamošiūnas, Mindaugas, Maciulevičius, Martynas, Šatkauskas, Saulius, Korf, Horst-Werner, Series editor, Boeckers, T.M., Series editor, Clascá, Francisco, Series editor, Kmiec, Z., Series editor, Singh, Baljit, Series editor, Sutovsky, Peter, Series editor, Timmermans, Jean-Pierre, Series editor, Kulbacka, Julita, editor, and Satkauskas, Saulius, editor

Published
2017
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16. Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer.

Author

Urbanskas, Ernestas, Jakštys, Baltramiejus, Venckus, Justinas, Malakauskaitė, Paulina, Šatkauskienė, Ingrida, Morkvėnaitė-Vilkončienė, Inga, and Šatkauskas, Saulius

Subjects
CHO cell, SQUARE waves, GENE expression, CELL survival, ELECTRIC fields, ELECTROPORATION
Abstract

Electroporation is a method that shows great promise as a non-viral approach for delivering genes by using high-voltage electric pulses to introduce DNA into cells to induce transient gene expression. This research aimed to evaluate the interplay between electric pulse intensity and 100 µs-duration pulse numbers as an outcome of gene electrotransfer efficacy and cell viability. Our results indicated a close relationship between pulse number and electric field strength regarding gene electrotransfer efficacy; higher electric pulse intensity resulted in fewer pulses needed to achieve the same gene electrotransfer efficacy. Subsequently, an increase in pulse number had a more negative impact on overall gene electrotransfer by significantly reducing cell viability. Based on our data, the best pulse parameters to transfect CHO cells with the pMax-GFP plasmid were using 5 HV square wave pulses of 1000 V/cm and 2 HV of 1600 V/cm, correspondingly resulting in 55 and 71% of transfected cells and maintaining 79 and 54% proliferating cells. This shows ESOPE-like 100 µs-duration pulse protocols can be used simultaneously to deliver cytotoxic drugs as well as immune response regulating genetically encoded cytokines. [ABSTRACT FROM AUTHOR]

Published
2024
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25. Affective Engagement to Virtual and Live Lectures

Author

Kasperiuniene, Judita, Jariwala, Meet, Vaskevicius, Egidijus, Satkauskas, Saulius, Diniz Junqueira Barbosa, Simone, Series editor, Chen, Phoebe, Series editor, Du, Xiaoyong, Series editor, Filipe, Joaquim, Series editor, Kara, Orhun, Series editor, Kotenko, Igor, Series editor, Liu, Ting, Series editor, Sivalingam, Krishna M., Series editor, Washio, Takashi, Series editor, Dregvaite, Giedre, editor, and Damasevicius, Robertas, editor

Published
2016
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50. Electrospray Mediated Localized and Targeted Chemotherapy in a Mouse Model of Lung Cancer

Author

Ruzgys, Paulius, primary, Böhringer, Stephan, additional, Dokumaci, Ayse Sila, additional, Hari, Yvonne, additional, Schürch, Christian M., additional, Brühl, Frido, additional, Schürch, Stefan, additional, Szidat, Sönke, additional, Riether, Carsten, additional, Šatkauskas, Saulius, additional, Geiser, Thomas, additional, Hradetzky, David, additional, and Gazdhar, Amiq, additional

Published
2021
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