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Your search keyword '"ŠPUNDA, Rudolf"' showing total 14 results
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14 results on '"ŠPUNDA, Rudolf"'

2. Viability of Human Arterial Grafts Monitored by Comet Assay.

Author

PILIN, Alexander, MAZURA, Ivan, LAINKOVÁ, Radka, SALMAY, Myroslav, MĚŘIČKA, Pavel, PECHA, Ondřej, JANOUŠEK, Libor, GRUS, Tomáš, ŠPUNDA, Rudolf, LINDNER, Jaroslav, and ŠPAČEK, Miroslav

Subjects
ARTERIAL grafts, ELECTROPHORESIS, VISUALIZATION, CELL survival, ORGAN donors, HOMOGRAFTS
Abstract

An analytical method for studying DNA degradation by electrophoresis after cell lysis and visualization of DNA fragments with fluorescent dye, comet assay, was used to evaluate the viability of the endothelial layer of human arterial grafts with the aim of identifying the procedure that will least damage the tissue before cryopreservation. Four groups of samples were studied: cryopreserved arterial grafts that were thawed in two different ways, slowly lasting 2 hours or rapidly for approx. 7 minutes. Arterial grafts that were collected as part of multiorgan procurement with minimal warm ischemia time. Cadaveric grafts were taken as part of the autopsy, so they have a more extended period of warm ischemia. The HeadDNA (%) parameter and others commonly used parameters like TailDNA (%). TailMoment, TailLength, OliveMoment, TailMoment to characterize the comet were used to assess viability in this study. The ratio of nondecayed to decayed nuclei was determined from the values found. This ratio for cadaveric grafts was 0.63, for slowly thawed cryopreserved grafts 2.9, for rapidly thawed cryopreserved grafts 1.9, and for multi-organ procurement grafts 0.68. The results of the study confirmed the assumption that the allografts obtained from cadaveric donors are the least suitable. On the other hand, grafts obtained from multiorgan donors are better in terms of viability monitored by comet assay. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Cell Viability Assessment Using Fluorescence Vital Dyes and Confocal Microscopy in Evaluating Freezing and Thawing Protocols Used in Cryopreservation of Allogeneic Venous Grafts

Author

Měřička, Pavel, primary, Janoušek, Libor, additional, Benda, Aleš, additional, Lainková, Radka, additional, Sabó, Ján, additional, Dalecká, Markéta, additional, Prokšová, Petra, additional, Salmay, Myroslav, additional, Špunda, Rudolf, additional, Pecha, Ondřej, additional, Jandová, Miroslava, additional, Gregor, Jiří, additional, Štěrba, Lubomír, additional, Špaček, Miroslav, additional, and Lindner, Jaroslav, additional

Published
2021
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6. Immunosuppressive protocols after cryopreserved aortal allotransplantation in rats

Author

Špunda, Rudolf, Špaček, Miroslav, Moláček, Jiří, and Rohn, Vilém

Subjects
antibody-mediated rejection, kryokonzervace, cell-mediated rejection, anti-MHC class II antibody, protilátky anti-MHC I. třídy, protilátková imunitní odpověď, buněčná imunitní odpověď, tacrolimus, cryoconservation, protilátky anti-MHC II. třídy, anti-MHC class I antibody, takrolimus, arteriální alloštěp, arterial allograft
Abstract

The aim of our study was to simulate in rats all aspects and techniques used in our new clinical program of cryopreserved alloarterial transplantation and investigate the influence of two immunosuppressive protocols with tacrolimus on acute rejection of these allografts. Cryopreserved abdominal aortic grafts were transplanted between Brown-Norway and Lewis rats. Tacrolimus (0,2 mg/kg daily) was administered from day 1 to day 30 (TAC1) or from day 7 to day 30 (TAC7), respectively. No immunosuppressed isogeneic (ISO) and allogeneic (ALO) rats combination served as control. Aortal wall destruction and infiltration by immunocompetent cells (MHC II+ cells of recipient origin) was studied on day 30 after transplantation. Flow cytometry was used for the analysis of day 30 sera for the presence of donor specific anti-MHC class I and II antibodies. The aortal allografts in both immunosuppressed groups showed regular morphology of aortal wall with no depositions of immunoglobulin G on day 30. The adventitial infiltration of non-immunosuppressed aortal allografts by MHC class II positive cells of recipient origin was significantly higher (ALO 20,7±6,7 cells, P

Published
2019

7. Current vascular allograft procurement, cryopreservation and transplantation techniques in the Czech Republic

Author

Špaček, Miroslav, primary, Měřička, Pavel, additional, Janoušek, Libor, additional, Štádler, Petr, additional, Adamec, Miloš, additional, Vlachovský, Robert, additional, Guňka, Igor, additional, Navrátil, Pavel, additional, Thieme, Filip, additional, Špunda, Rudolf, additional, Burkert, Jan, additional, Staffa, Robert, additional, Němec, Petr, additional, and Lindner, Jaroslav, additional

Published
2019
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9. Organization model for allotransplantations of cryopreserved vascular grafts in Czech Republic

Author

Špaček, Miroslav, primary, Měřička, Pavel, additional, Janoušek, Libor, additional, Štádler, Petr, additional, Adamec, Miloš, additional, Vlachovský, Robert, additional, Guňka, Igor, additional, Navrátil, Pavel, additional, Thieme, Filip, additional, Mitáš, Petr, additional, Špunda, Rudolf, additional, Špatenka, Jaroslav, additional, Staffa, Robert, additional, Němec, Petr, additional, and Lindner, Jaroslav, additional

Published
2018
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10. Juxtarenal Modular Aortic Stent Graft Infection Caused by Staphylococcus aureus

Author

Novotný, Róbert, Mitáš, Petr, Hlubocký, Jaroslav, Hrubý, Ján, Slautin, Andrey, Špunda, Rudolf, and Lindner, Jaroslav

Subjects
Article Subject
Abstract

Introduction. We are presenting a case report of an infected modular abdominal stent graft. Case Presentation. A 67-year-old male patient three years after Cook’s modular abdominal aortic aneurysm (AAA) graft implantation for juxtarenal AAA with an implantation of a stent extension into the right common iliac artery for type Ib endoleak. The patient was admitted into our center in severe condition with suspected retroperitoneal bleeding. Computed tomography angiography (CTAG) confirmed retroperitoneal bleeding in the right common iliac artery. An urgent surgical revision was indicated; destructed arterial wall around the stent extension in the right common iliac artery was discovered. Due to the severe state of health of the patient, a resection of the infected stent and affected arterial wall was performed, followed by an iliac-femoral crossover bypass. The patient was transported to the intensive care unit with hepatic and renal failure, with maximal catecholamine support. Combined antibiotic treatment was started. The patient died five hours after the procedure. The cause of death was multiorgan failure caused by sepsis. Hemocultures and perioperative microbiological cultures showed the infection agent to be Staphylococcus aureus methicillin sensitive. Conclusion. Stent graft infection is a rare complication. Treatment is associated with high mortality and morbidity.

Published
2016
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11. Isolated Perfusion of the Upper Extremity with TNF-α – Double Venous Cannulation

Author

Špaček, Miroslav, primary, Mitáš, Petr, additional, Vočka, Michal, additional, Lacina, Lukáš, additional, Hodková, Gabriela, additional, Špunda, Rudolf, additional, Kodet, Ondřej, additional, Krajsová, Ivana, additional, Petruželka, Luboš, additional, Trnka, Jiří, additional, Kubinyi, Jozef, additional, Matějovský, Zdeněk, additional, Lambert, Lukáš, additional, and Lindner, Jaroslav, additional

Published
2017
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12. A rationale for early extracorporeal membrane oxygenation in patients with postinfarction ventricular septal rupture complicated by cardiogenic shock

Author

Rob, Daniel, primary, Špunda, Rudolf, additional, Lindner, Jaroslav, additional, Rohn, Vilém, additional, Kunstýř, Jan, additional, Balík, Martin, additional, Rulíšek, Jan, additional, Kopecký, Petr, additional, Lipš, Michal, additional, Šmíd, Ondřej, additional, Kovárník, Tomáš, additional, Mlejnský, František, additional, Linhart, Aleš, additional, and Bělohlávek, Jan, additional

Published
2017
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