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iv ABSTRACT Benthic diatoms are due to a number of positive characteristics (such as high abundances, diversified communities, sensitive responses to environmental conditions, capturing long-term changes in the environmental conditions) currently the most commonly used bioindicators of the ecological status of freshwater ecosystems. However, due to problems associated with the use of traditional methods based on species composition (time-consuming identification, presence of species complexes, and requirements for calibration of water quality indexes for geographical regions) searching for alternative methods has been induced. The monitoring of the size structure of diverse diatom communities has been proposed as one of the methods. This approach would mainly eliminate the necessity of time-consuming determination of species. The main purpose of this thesis was therefore to determine the relationship between the size structure of benthic diatom communities, expressed as the relative biovolume, and selected environmental factors (pH, conductivity, habitat type) within freshwater lentic habitats. Furthermore, the variability of biovolume in dependence on environmental variables was compared with the change in species composition. If there would be a similar response pattern of both the biovolume and species...