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Kupres cheese is a traditional Bosnian full-fat hard cheese. This paper deals with the change of protein and fatty acid profiles, mineral content and antioxidant potential during the period of three months of its ripening. Proteolysis was monitored by the electrophoresis of total extracts and water-soluble fractions under reducing conditions, whereas fatty acid profiles were determined by gas chromatography. The mineral content in the ripened cheeses was determined by using methods of Inductively coupled plasma - optical emission spectrometry (ICP-OES) for microelements and Inductively coupled plasma mass spectrometry (ICP-MS) for microelements, respectively. Ripening of Kupres cheese during three months is characterised by a relatively slow proteolysis, especially during the first month and by the increase in desirable fatty acid content. After 30 days of ripening, the WSN/TN and TCA/TN contents increased from 8.23% and 2.25% to 10.15% and 3.59%, respectively. Further, ripening induced higher increase of these parameters and more intensive degradation of the major caseins, especially αs-CN. After three months of ripening, residual level of αs-CN and β-CN was 12.02% and 80.96%, respectively. Three month-old cheese had improved indexes of lipid quality as well as improved reducing power and ability to scavenge free radicals. Ripening affected the content of Ca, P and Na whereas no significant changes of the other macro- and microelements of Kupres cheese were observed., Kupreški sir je tradicionalni bosanski punomasni tvrdi sir. U ovom istraživanju praćene su promjene profila proteina i masnih kiselina, udjela mineralnih tvari i antioksidacijskog potencijala tijekom tri mjeseca njegovog zrenja. Proteoliza je praćena elektroforezom ukupnih ekstrakata i frakcija topljivih u vodi u redukcijskim uvjetima, dok su profili masnih kiselina određivani plinskom kromatografijom. Udjel mineralnih tvari u zrelim sirevima određen je metodom induktivno spregnute plazme i optičke emisijske spektrometrije (ICP-OES) koja je korištena za makroelementie, kao i spektrometrijom masa uz induktivno spregnutu plazmu (ICP-MS) koja je korištena za mikroelemente. Zrenje kupreškog sira karakterizira relativno spora proteoliza tijekom prvog mjeseca, povećanje udjela poželjnih masnih kiselina i poboljšanje ostalih zdravstvenih indeksa masnih kiselina. Zrenje također poboljšava ukupni antioksidacijski kapacitet i reducirajuću sposobnost sira. Nakon 30 dana zrenja udjel VSN/TN i TCA/TN raste s 8,23 % i 2,25 % na 10,15 % i 3,59 %. Daljnje je zrenje uzrokovalo intenzivnije povećanje ovih parametara i intenzivniju degradaciju glavnih kazeina, posebno αs-CN. Nakon tri mjeseca zrenja rezidualni nivo αs-CN i β-CN iznosio je 12,02 %, odnosno 80,96 %. Sir koji je dozrijevao tri mjeseca imao je poboljšane indekse kvalitete lipida, kao i poboljšanu redukcijsku moći sposobnost uklanjanja slobodnih radikala. Zrenje je izazvalo značajne promjene udjela Ca, P i Na, ali nije utjecalo na udjele ostalih makro- i mikroelemenata kupreškog sira.

The programmes for pesticide residues monitoring in food which are continually carried out in the world ensure that the consumers are not exposed to pesticide residues as well as that only the registered compounds are used in the production. All the member countries of the EU are obliged to observe the official control of pesticide residues in food, in accordance with the Regulations EC 396/2005. At present, in the Republic of Serbia, the programme for monitoring the pesticide residues in food is being prepared as the results of numerous analyses of random samples of fruit, in the market over the past years justify the introduction of such a programme. Programi praćenja ostataka pesticida u hrani koji se kontinuirano sprovode u svetu osiguravaju potrošače da ne budu izloženi nepoželjnom nivou ostataka pesticida, kao i da se u proizvodnji koriste samo registrovana jedinjenja. Sve zemlje članice EU su dužne da sprovode zvaničnu kontrolu prisustva ostataka pesticida u hrani, u saglasnosti sa Regulativom EC 396/2005. U Republici Srbiji je u pripremi monitoring program za ostatke pesticida u hrani, a rezultati analiza većeg broja slučajno izabranih uzoraka voća, na našem tržištu, tokom proteklih godina ukazuju na opravdanost uvođenja ovakvog programa.