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Abstract The “4.2 ka event” is a commonly described abrupt climate excursion that occurred about 4200 years ago. However, the extent to which this event is coherent across regional and larger scales is unclear. To objectively assess climate excursions in the Holocene we compile 1142 paleoclimate datasets that span all continents and oceans and include a wide variety of archive and proxy types. We analyze these data to determine the timing, significance and spatial imprint of climate excursions using an objective method that quantifies local, regional and global significance. Site-level excursions in temperature and hydroclimate are common throughout the Holocene, but significant global-scale excursions are rare. The most prominent excursion occurred 8200 years ago, when cold and dry conditions formed a large, significant excursion centered in the North Atlantic. We find additional significant excursions between 1600 and 1000 years ago, which agree with tree-ring data and annual-scale paleoclimate reconstructions, adding confidence and context to our findings. In contrast, although some datasets show significant climate excursions 4200 years ago, they do not occur in large, coherent spatial regions. Consequently, like most other periods in the Holocene, the “4.2 ka event” is not a globally significant climate excursion.

The research on new and innovative solutions, technologies and products carried out on an industrial scale is the most reliable method of verifying the validity of their implementation. The results obtained in this research method give almost one hundred percent certainty although, at the same time, the research on an industrial scale requires the expenditure of the highest amount of money. Therefore, this method is not commonly applied in the industrial practices. In the case of the decision to implement new and innovative technologies, it is reasonable to carry out industrial research, both because of the cognitive values and its economic efficiency. Research on an industrial scale may prevent investment failure as well as lead to an improvement of technologies, which is the source of economic efficiency. In this paper, an evaluation model of economic efficiency of the industrial scale research has been presented. This model is based on the discount method and the decision tree model. A practical application of this proposed evaluation model has been presented based on an example of the coal charge pre-drying technology before coke making in a coke oven battery, which may be preceded by industrial scale research on a new type of coal charge dryer.

Smooth brome (Bromus inermis L.) is a species of perennial grass with growing economic importance. Initially, this species had attracted interest as a source of animal feed. Over the years, the interest in smooth brome increased significantly due to the growing knowledge about its advantages. The aim of this study was to explore the contemporary significance of smooth brome. This plant is characterized by a high tolerance to many negative environmental factors, such as periodic droughts, low temperatures and salinity, which contributes to its constant presence in the landscape of many countries. The moderate soil requirements of smooth brome, combined with the effective use of soil resources and rational nutrient utilization, contribute to high biomass yields that can reach 13 t/ha DM. The usefulness of this grass species in various management systems has been recognized in numerous research studies. Smooth brome can generate benefits in many branches of the economy. This efficient energy plant is used in paper production, and it is also recommended for the protection of fallow land or the reclamation of degraded land. Smooth brome prevents erosion, enhances biodiversity, and provides shelter for many animal species. This species fits well into the current assumptions of agricultural policy and increasingly demanding environmental standards. According to the latest guidelines, modern agriculture should pursue economic and environmental goals simultaneously. In this context, smooth brome constitutes a valuable link in sustainable development. Due to its numerous advantages, smooth brome not only provides high-quality feed and biomass but also effectively sequesters CO2, improves soil fertility and enhances biodiversity, which makes it an important element of agriculture and environmental protection.

The proper fallowing of soil maintains or even improves its yield potential. The aim of this research was to compare five methods of soil protection with high production potential on the yield and quality of strategic plants. The tested methods consisted of five variants: bare fallow—BF; natural fallow—NF; fodder galega (Galega orientalis Lam.)—FG; a mixture of fodder galega (Galega orientalis Lam.) with smooth brome (Bromus inermis)—FG+SB; and smooth brome (Bromus inermis)—SB. The soil had been set aside for 9 years, after which time the fallows were terminated and the fields were cropped with winter oilseed rape, winter wheat, and spring wheat in three consecutive years. After the end of fallowing, the content of Nog. and Ctot., pH, and forms of available macro- and microelements in the soil were determined. The influence of each type of fallow on the yield of seeds/grain, straw, total protein, crude fat, and the content of macronutrients in the seeds/grain and straw of the grown crops was determined. Regarding the yields of the crops, the best solution was long-term soil protection via sowing fodder galega or a mixture of fodder galega and smooth brome. A field previously maintained as a fallow with these plants (singly or in combination) could produce over twice-as-high yields of wheat and oilseed rape as those harvested from a field established on bare fallow. The yields of the cereals and oilseed rape obtained in this study prove that food security and environmental protection issues can be reconciled. The methods for protecting farmland temporarily excluded from agricultural production presented in this paper correspond perfectly to the framework of the Green Deal for Europe. Arable land excluded from cultivation can be used to overcome new challenges facing modern agriculture.

The aim of this incubation experiment was to evaluate the effect of Tenebrio molitor L. frass on selected chemical and microbiological properties of acid peat. The optimal rate of mealworm frass in the substrate for growing ornamental trees and shrubs was determined. Acid peat was fertilized with frass or urea at five nitrogen (N) rates: 0, 50, 100, 200, and 400 mg dm−3. Mineral N content and electrical conductivity increased, and calcium content decreased in peat with increasing N rates. Similarly to urea, frass increased the ammonification rate at the beginning of incubation and the nitrification rate from the second week of incubation. Higher frass rates increased the abundance of plant-available nutrients (N, P, Mg, K, and Na) in acid peat. Frass undesirably decreased the counts of bacteria with antagonistic activity against soil-borne plant pathogens. Regarding the abundance of functional genes, the optimal N rate was 100 mg dm−3, which promoted the growth of N-fixing and chitinolytic bacteria. Higher N rates promoted the development of aerobic spore-forming bacteria, which produce antibiotics that can be used as biocontrol agents. Moderate fertilizer rates contributed to N accumulation in bacterial biomass. These preliminary findings, which indicate that insect frass can partially replace mineral fertilizers, are promising and can be used in pot and field experiments testing various plant species.

Abandonment of crop production on agricultural lands for several or more years is a widespread practice not only in Europe but also around the world. Economic and political considerations lead to the abandonment of crop production on the poorest lands, although sometimes agriculturally valuable lands are also excluded from farming. Fallow land can be afforested, designated as a dedicated protection area, exposed to natural succession, or used to grow biomass for energy purposes. However, the most important role of agricultural land should be to ensure food safety. The set-aside land with high production potential should be treated in a special way. While lying fallow, the soil can have its fertility sustained or even improved considerably. To this aim, uncultivated land should be properly protected by growing a permanent cover of plant species which will have a positive influence on the soil’s physical, chemical, and biological characteristics. Depending on the geographical location, different plant species will have a beneficial effect on set-aside soil. Given economic and environmental considerations, the best solution is to sow a mixture of grasses and legumes, which can improve substantially the biodiversity on fallow fields, raise the soil’s fertility, ensure high CO2 sequestration ratios, and influence beneficially the soil’s nutritional status and nutrient management. Soil protection can be provided for many years with little effort. The most recent reports implicate that it is possible to achieve several economic and environmental aims simultaneously in the course of the management of land excluded from agricultural production. These aims include the improvement of biodiversity, control of greenhouse gas emissions, generation of energy, and readiness to resume production of commodity plants. Proper management of fallows corresponds well with the challenges defined in the Green Deal for Europe or the US Green New Deal.

Climate change causes alterations in lake systems, thus influencing lake biota. Studies of phytoplankton with short generation times and seasonal replacement provide an opportunity for assessing how aquatic organisms respond to changes in environmental conditions. This study explores the potential of chrysophyte cysts as indicators of seasonal and interannual changes in meteorological conditions in Lakes Łazduny and Rzęśniki, located in northern Poland. Monthly chrysophyte cyst data from sediment traps, biweekly limnological and hydrochemical data, and daily meteorological data were used to find and explain the relationships between chrysophyte cysts and changes in meteorological conditions. We showed that cyst seasonality indirectly corresponds to meteorological conditions acting through changes in the mixing regimes, which in turn influences nutrient and light availability. Statistical analyses revealed that the taxonomic structure and interannual variability of chrysophyte cysts are dependent on multiple variables. Nevertheless, air temperature was the most important meteorological variable influencing cyst assemblages. Cysts were indicative of different periods of lake physical structure, suggesting the potential of chrysophyte cysts in paleoclimatic studies.

Varved lake sediments are well known archives used for paleoenvironmental reconstructions. They provide continuous, high-resolution and calendar-year chronologies of past environmental changes. From lakes Kamenduł and Perty, located in the Suwałki Landscape Park, short cores of laminated sediments were collected and investigated using microfacies analysis and X-ray fluorescence (XRF) scanning. Sediments of Lake Kamenduł form biogenic (calcite) varves with pale lamina containing precipitated calcite and dark lamina composed of mixed organic and clastic material. There is a visible difference between varves in the lower and the topmost part of the core. The varve thickness and clastic material content decrease upcore. Analysed sediments of Lake Perty also show laminated structures which are biogenic varves composed of calcite laminas interbedded with layers of mixed material and dark laminas containing mostly organic material with some clastic particles. Our study shows that further analysis of sediments from these lakes may provide valuable information about past land use changes in their catchments as well as changes in the water column, i.e. mixing regimes and redox conditions.

Typically, the description of varve microfacies is based on microscopic sedimentary structures, while standard grain-size analysis is commonly applied with lower resolution. Studies involving a direct comparison of varve microfacies and particle-size distributions, common for clastic environments, are scarce for biogenic varves. In this study, we analyzed nine-year resolution grain-size data from Lake Żabińskie (northeastern Poland) to detect differences between varve microfacies. Six varve microfacies were differentiated using grain-size distributions and sedimentological attributes (calcite layer thickness, dark layer thickness, mass accumulation rate). However, changes in particle-size distributions between different varve types are relatively small and indicate a similar source for the material deposited. Decomposition of grain-size distributions with the end-member approach allows recognition of relative changes for the deposition of allochthonous (mineral) and autochthonous (carbonates, (hydr)oxides) components. Grain-size data suggest that sources of allochthonous material remained constant, while varve formation was controlled mostly by in-lake processes.

This paper presents the results of a 3-year field experiment conducted on soil with moderate levels of boron (B) in north-eastern Poland to determine the influence of foliar application of B (0, 150 and 300 g ha-1) on the yield components, yield, mineral composition, nutritional value and feed value of winter oilseed rape seeds. Foliar application of B at the beginning of bud formation in winter oilseed rape increased seed yield by 0.19 (3%) at the lower B fertilization level to 0.26 Mg ha-1 (4%) at the higher B fertilization level. The observed increase in the yield of winter oilseed rape seeds in response to foliar application of B can be attributed to this micronutrient’s positive influence on seed production in siliques. Foliar fertilization with B increased B content and decreased Zn and Fe levels in the seeds of winter oilseed rape. When B fertilizer was applied at the rate of 150–300 g ha-1, the N and Ca content of the evaluated seeds increased. The fertilizer improved the nutritional value (crude fat content, fatty acid concentrations) of seeds, but deteriorated their feed value (total protein content, acid detergent fiber concentrations, neutral detergent fiber concentrations, quantitative and qualitative composition of glucosinolates).

Selenium is considered to be an element essential for the proper functioning of animal and human organisms. Its most important functions include anticancerogenic activity and beneficial effects on the human circulatory system, as well as its role during the perinatal period in animals. This article discusses a significant role selenium plays in animal organisms, the soil content of this element in soils, and a prospect for Se enrichment in plants to levels covering the dietary needs of animals. The incidence of selenium deficiency in animals can be reduced by enriching fodder with supplements containing selenium in mineral compounds, which are much less assimilated by living organisms than its organic forms. The paper also provides information on the content and bioavailability of selenium in Polish soils and presents the average concentrations of this element in different species of plants. While the methods of introducing selenium into the soil-plant-animal-human food chain have been extensively studied during the recent years, the practice of fertilizing plants with this element is still less known. The basic source of selenium for plants is soil, yet most of the soils in Poland are poor in selenium. It has been demonstrated that the application of 5 to 10 g ha-1 Se in the cultivation of cereals, meadows and pastures may increase its content in plants to the level covering the needs of animals for this element. The effectiveness of Se enrichment in plants to a level safe in animal nutrition may depend not only on the dose or chemical form of selenium applied, but also on the species and varietal properties of plants, the time of application and the application method itself. Due to a small difference between the optimal and toxic content, plant fertilization with selenium should be preceded by a study on its content in soil, as well as the ability of plants to accumulate this element. [ABSTRACT FROM AUTHOR]

This study compares different methods to count biogenic varves in Lake Żabińskie in northeastern Poland. To perform this comparison, we used 2000 years-long varved sediment record from this lake because of the well-preserved laminations as well as the independent age controls, i.e. 29 AMS 14 C dates, 137 Cs activity peaks and volcanic glass shards (1875 AD Askja cryptotephra). Microscopic investigations of thin sections allowed us to identify six major varve microfacies along the sediment profile. Using these microfacies, we tested three counting methods and different approaches of counting uncertainty estimation. These methods provided comparable numbers of varves (1943 ± 30, 2034 ± 34, 2028 +34 / -53 ) and similar shapes in the age-depth relations. However, in two methods, we found possible disadvantages in arbitrary assumptions and subjective decisions in the process of chronology building, procedures leading to age underestimation as well as problems with reliable and objective estimations of counting uncertainty. Based on these results, we propose the counting method which include analyses of major varve microfacies and three independent countings without the need for varve-by-varve microscopic investigations. This method provided acceptable results (<5% uncertainty) and was more objective and less time-consuming. Semi-automatic counting using μXRF Ca record in the sediments was also tested and provided results that can serve as a first approximation of the number of varves. Our study also indicates that different counting strategies may lead to different uncertainty estimations, which may be important in the reliable interpretation of proxy data. [ABSTRACT FROM AUTHOR]

Minerals, in particular micronutrients such as copper, manganese, zinc, selenium and chromium, deliver health benefits for high-yielding dairy cows. The availability of nutrients is particularly important during the transition period (between 3 weeks prepartum to 3-4 weeks postpartum), which is a highly vulnerable time in the production cycle that determines the health status of lactating cows, their milk yield and reproductive performance. In cows, prolonged mineral deficiency can produce subclinical symptoms of nutrient deficiency and, if untreated, can lead to a clinical presentation of the disorder. Less severe deficiencies, in particular micronutrient deficiencies, are more difficult to identify because they induce only minor functional and structural changes in the body, but together with energy metabolism disorders, they can lead to oxidative stress and immunosuppression, in particular in the transition period. Micronutrient supplements reduce the harmful effects of free radicals because the majority of micronutrients form the active centers of antioxidant enzymes. Dietary supplementation promotes reproductive performance, healthy fetal development and immunity. Micronutrients alleviate bacterial infections associated with mastitis, lower somatic cell counts in milk and minimize the risk of metabolic diseases caused by a negative energy balance. This study discusses the role of the major micronutrients in cattle nutrition and their effect on the health status of transition cows. [ABSTRACT FROM AUTHOR]

The aim of this study was to determine the effect of different systems of foliar fertilization on the yield of winter oilseed rape (Brassica napus L.), the mineral composition of post-harvest biomass and the processing suitability of seeds. In winter oilseed rape grown on boulder clay, intensified foliar fertilization increased seed yield (by 0.43-0.69 Mg ha-1 87% dry matter, DM), straw yield (by 0.59-1.69 Mg ha-1 DM), and decreased the harvest index (by 2-3%). The seeds of winter oilseed rape accumulated more N, P, Ca, Zn, Cu, Mn and Fe than straw. The concentrations of P, Mg and S were higher in straw than in seeds. Foliar fertilization increased the N, P and K content (by 2.0, 0.35 and 1.15 g kg-1 DM, respectively) and decreased the Mg and S content of straw (by 2.08 and 0.77 g kg-1 DM, respectively). A chemical analysis of seeds revealed significant changes only in the concentrations of K, S (increase by 0.57 and 0.55 g kg-1 DM, respectively) and P (decrease by 0.90 g kg-1 DM, respectively) under the influence of foliar fertilization. Intensified foliar fertilization increased the concentrations of Cu and Zn and decreased the levels of Mn and Fe in straw and seeds. The nutritional value of seeds deteriorated (crude fat content decreased by around 5.5 g kg-1 DM, but the composition of fatty acids (FAs), and the proportions of saturated FAs, monounsaturated FAs and polyunsaturated FAs were not significantly altered) with an increase in foliar fertilization levels. Foliar application of macronutrients and micronutrients improved the feed value of winter oilseed rape seeds. Intensified foliar fertilization reduced the content of alkenyl (by approx. 47%) and indole (by approx. 6%) glucosinolates in seeds, mostly due to a decrease in the concentrations of progoitrin, gluconapin and 4-OH-glucobrassicin. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Elementology is the property of Journal of Elementology - issued by Polish Magnesium Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Elementology is the property of Journal of Elementology - issued by Polish Magnesium Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Elementology is the property of Journal of Elementology - issued by Polish Magnesium Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Here, we present a dataset of a decade-long monitoring project of Lake Żabińskie, a hardwater eutrophic lake in northeast Poland. The lake contains annually laminated (varved) sediments that form a unique archive of past environmental variability. Regular measurements of the lake water physical and chemical characteristics were done using a multiparameter sonde and a set of temperature sensors deployed in the water column. Seasonal variability in sediment fluxes was documented by a sediment trap. Field sampling provided information about the hydrochemistry of incoming streams and of the lake outflow. The overall monitoring program was designed to assess relationships among meteorological conditions, limnological processes, and modern sedimentation and to answer the question of whether meteorological and limnological phenomena can be precisely tracked with varves. However, this dataset can also be used for modeling physical and biogeochemical processes in lakes. The dataset is archived at https://doi.org/10.34808/w8w7-9x34 (Tylmann et al., 2023).

Varved lake sediments are exceptional archives of paleoclimatic information due to their precise chronological control and annual resolution. However, quantitative paleoclimate reconstructions based on the biogeochemical composition of biochemical varves are extremely rare, mainly because the climate–proxy relationships are complex and obtaining biogeochemical proxy data at very high (annual) resolution is difficult. Recent developments in high-resolution hyperspectral imaging (HSI) of sedimentary pigment biomarkers combined with micro X-ray fluorescence (µXRF) elemental mapping make it possible to measure the structure and composition of varves at unprecedented resolution. This provides opportunities to explore seasonal climate signals preserved in biochemical varves and, thus, assess the potential for annual-resolution climate reconstruction from biochemical varves. Here, we present a geochemical dataset including HSI-inferred sedimentary pigments and µXRF-inferred elements at very high spatial resolution (60 µm, i.e. > 100 data points per varve year) in varved sediments of Lake Żabińskie, Poland, over the period 1966–2019 CE. We compare these data with local meteorological observations to explore and quantify how changing seasonal meteorological conditions influenced sediment composition and varve formation processes. Based on the dissimilarity of within-varve multivariate geochemical time series, we classified varves into four types. Multivariate analysis of variance shows that these four varve types were formed in years with significantly different seasonal meteorological conditions. Generalized additive models (GAMs) were used to infer seasonal climate conditions based on sedimentary variables. Spring and summer (MAMJJA) temperatures were predicted using Ti and total C (Radj2=0.55; cross-validated root mean square error (CV-RMSE) = 0.7 ∘C, 14.4 %). Windy days from March to December (mean daily wind speed > 7 m s−1) were predicted using mass accumulation rate (MAR) and Si (Radj2=0.48; CV-RMSE = 19.0 %). This study demonstrates that high-resolution scanning techniques are promising tools to improve our understanding of varve formation processes and climate–proxy relationships in biochemical varves. This knowledge is the basis for quantitative high-resolution paleoclimate reconstructions, and here we provide examples of calibration and validation of annual-resolution seasonal weather inference from varve biogeochemical data.

Hypercholesterolemia is the main cardiovascular (CV) risk factor with a large body of evidence. Our aim was to assess the achievement of the main therapeutic goal of Low-Density Lipoprotein Cholesterol (LDL-C) in patients with a very high CV risk and a high-dose statin therapy. The study group consisted of 1413 consecutive patients hospitalised at the Upper-Silesian Medical Centre in Katowice due to acute myocardial infarction (AMI) treated with atorvastatin ≥ 40 mg or rosuvastatin ≥ 20 mg. The lipid profile was performed on admission and within 12 months after AMI. The main therapeutic goal was defined as LDL-C < 55 mg%. The study group (n = 1413) included 979 males (69.3%) with arterial hypertension (83.3%), diabetes (33.5%), peripheral artery disease (13.6%) and nicotinism (46.2%). In the study group, only 61 patients (4.3%) were additionally taking ezetimibe. During hospitalisation, the primary LDL-C goal was found in only 186 patients (13.2%). Subsequently, a follow-up lipidogram within 12 months was performed in 652 patients (46%), and the therapeutic goal was achieved in 255 patients (39%). There were 258 (18.26%) patients who died within 12 months after myocardial infarction. The lowest mortality rate was found in the subgroup of patients with LDL-C < 55 mg% during follow-up (11.02%). The primary lipid goal attainment among patients with a high-dose statin and a very high CV risk is low and far from the expected rate. Patients hospitalised for AMI should be given a combination of statin and ezetimibe more frequently. Low LDL-C levels measured at follow-up predict a lower risk of death at 12-month follow-up in a large group of patients.

This article presents results of a three-year field experiment laid out on medium textured soil, in north-eastern Poland. Winter oilseed rape was sprayed with foliar fertilisers AgravitaActive 48 (AA48) - NPK 8:8.7:16.6 and AgravitaActive70 (AA70) - NPK 10:22.7:6.6 at different phases of the crop’s vegetative growth (BBCH 14, BBCH 35). The impact of the fertilisers on the yield of seeds, straw, total protein, crude fat, and on the content of macro- and micronutrients in aerial parts (BBCH 20 and 89) and roots (BBCH 20) of oilseed rape was evaluated. Foliar nutrition of oilseed rape plants with AA48 and AA70 significantly increased the seed yield of oilseed rape. The best yield-stimulating effect was achieved after the application of AA48 in autumn. Fertilisation of oilseed rape in that season of the year caused an increase in seed yield by 430 kg ha-1. Each fertiliser contributed to a rise in the yield of protein and yield of crude fat but had no effect on the content of magnesium, copper, and zinc in seeds.

Fodder galega is a perennial, wintering plant, which in recent years has been gaining popularity, mainly because of its useful characteristics. Many researchers have noted its large yield potential and exceptional adaptability to various environmental conditions. The purpose of this study was to collect the most up-to-date knowledge about this valuable plant, a member of the Fabaceae family. Green fodder, especially that made from young plants, possesses very good biochemical parameters which allow it to be used in the nutrition of animals including poultry. Plantations of fodder galega can be set up on highly diverse soils, and in all systems of management. The yielding potential of this plant is appreciated on all farms: organic, extensive and conventional ones. Many scientific studies have demonstrated the usefulness of fodder galega for the protection of farmland that is periodically excluded from farming. In recent years, reports have been published suggesting the high potential of this plant for the acquisition of inexpensive energy from its biomass. Moreover, many scholars have emphasised the medicinal applications of fodder galega. The advantages of the cultivation of fodder galega are (1) its low nutritional requirements; (2) a lack of threats from the specialised pests; and (3) its positive effect on soil fertility. It is recommended that areas having undergone several years of fodder galega cultivation act as very good forecrop for plants with high nutritional requirements.

The purpose of this study was to evaluate the effect of ash from combustion of plant biomass of energy willow and Pennsylvania fanpetals on yields of willow grown as an energy crop and on soil properties. A three-year pot experiment was carried out on substrates with a loamy sand texture. Ash application rates were based on the potassium fertilisation demand. An incubation experiment was carried out to determine the effect of biomass-based ash on soil properties. Three soils with textural categories were incubated for 3 months with the ashes, the doses of which were determined on the basis of the hydrolytic acidity of soils (¼; ½ and 1.0 Hh). It was found that ashes generated from burning willow or Pennsylvania fanpetals can be applied instead of phosphorus, potassium and magnesium fertilisers in the cultivation of energy willow. The plant uptake of P, K and Mg from the ashes did not diverge from their absorption by plants when supplied with mineral salts. The application of these alkaline ashes will increase the soil content of phytoavailable forms of phosphorus, potassium and magnesium. The examined ashes enriched the soil with micronutrients.

The analysis of genetic material may be the only way to identify an unknown person or solve a criminal case. Often, the conditions in which the genetic material was found determine the choice of the analytical method. Hence, it is extremely important to understand the influence of various factors, both external and internal, on genetic material. The review presents information on DNA and RNA persistence, depending on the chemical and physical factors affecting the genetic material integrity. One of the factors taken into account is the time elapsing to genetic material recovery. Temperature can both preserve the genetic material or lead to its rapid degradation. Radiation, aquatic environments, and various types of chemical and physical factors also affect the genetic material quality. The substances used during the forensic process, i.e., for biological trace visualization or maceration, are also discussed. Proper analysis of genetic material degradation can help determine the post-mortem interval (PMI) or time since deposition (TsD), which may play a key role in criminal cases.

Hypercholesterolemia is the main cardiovascular (CV) risk factor with a large body of evidence. Our aim was to assess the achievement of the main therapeutic goal of Low-Density Lipoprotein Cholesterol (LDL-C) in patients with a very high CV risk and a high-dose statin therapy. The study group consisted of 1413 consecutive patients hospitalised at the Upper-Silesian Medical Centre in Katowice due to acute myocardial infarction (AMI) treated with atorvastatin ≥ 40 mg or rosuvastatin ≥ 20 mg. The lipid profile was performed on admission and within 12 months after AMI. The main therapeutic goal was defined as LDL-C < 55 mg%. The study group ( n = 1413) included 979 males (69.3%) with arterial hypertension (83.3%), diabetes (33.5%), peripheral artery disease (13.6%) and nicotinism (46.2%). In the study group, only 61 patients (4.3%) were additionally taking ezetimibe. During hospitalisation, the primary LDL-C goal was found in only 186 patients (13.2%). Subsequently, a follow-up lipidogram within 12 months was performed in 652 patients (46%), and the therapeutic goal was achieved in 255 patients (39%). There were 258 (18.26%) patients who died within 12 months after myocardial infarction. The lowest mortality rate was found in the subgroup of patients with LDL-C < 55 mg% during follow-up (11.02%). The primary lipid goal attainment among patients with a high-dose statin and a very high CV risk is low and far from the expected rate. Patients hospitalised for AMI should be given a combination of statin and ezetimibe more frequently. Low LDL-C levels measured at follow-up predict a lower risk of death at 12-month follow-up in a large group of patients.