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Internal colonizers of bacteria were isolated from gut after partial surface sterilization using UV irradiation and the subsequent dispersal treatment. Genomic DNA extraction from each isolate was performed with a FTA TM card (Whatman, UK), and then PCR-amplified, targeting 16S rDNA fragments with universal primer pairs. The PCR products were purified and then sequenced to identify a series of bacterial strains collected during this study. Most of the gut floras were members of the Aeromonas group commonly distributed in aquatic habitats, whilst others were identified as Paucibacter and Acidovorax, both may degrade native and manmade toxic materials such as cyanotoxin and dioxin, respectively. These normal floras of invertebrates inhabiting slow sand filters contribute not only to purify water but may decompose unfavorable toxic substances, presumably reducing potential health risks during drinking water production.

Larvae of African chironomid Polypedilum vanderplanki represent highly developed mechanism of anhydrobiotic conservation of metabolism which allowing them to stay alive for many years in the absence of water and be utilized as a model organisms in long-term projects, including the spaceflight. In this paper we shortly overview the main properties of anhydrobiosis of the larvae and describe the main space experiments where the insect was used as a model organism for investigation of influence of spaceflight environment of latent stages of invertebrates., 資料番号: AA0063706087

西南日本において海産ユスリカの生態に関わる研究を行った。その結果，計20種が採集され，垂直分布では，潮間帯上部あるいは中部域のみに分布する種は少なかったが，下部域のみに分布する種は多かった。基質については岩石面や泥中のみに棲む種は少なかったのに対し，海藻類のみに棲む種は多かった。また，地理的分布では南西諸島や本土外洋沿岸域のみに分布する種は少なかった。一方，数種は，瀬戸内海沿岸と本土外洋沿岸域，本土外洋沿岸域と南西諸島，あるいは全地域に分布することが分かった。また，羽化時期については，Dicrotendipes enteromorphaeは夏季・秋季のみ，S. endocladiaeは周年出現し，他種は秋季・冬季のみあるいは冬季・春季のみに出現した。これらのことから，潮間帯には様々な生活様式を持つ多くの種のユスリカが棲息し，生態系において重要な役割を果たしていることが示唆された。, Ecological studies were conducted on marine chironomids in south-western Japan. A total of 20 species was collected. For vertical distribution, only a few species was distributed only at high or middle intertidal zone while many species were distributed only at low zone. For substrate preference, some species were collected only on rocks or in mud while many species were collected only in seaweeds. For geographical distribution, a few species was collected only in the southwestern islands or in the oceanic coast in the Japanese main islands. In contrast, some species were collected in the Seto Inland Sea and the oceanic coast in the main islands, in the oceanic coast in the main islands and the southwestern islands, or in all areas. For seasonal emergence, Dicrotendipes enteromorphae was collected only in summer and autumn, and Semiocladius endocladiae was collected in all seasons. Other species were collected only in autumn and winter, or only in winter and spring. These results suggest that many chironomid species with a variety of lifestyles dwell in intertidal zones and have some important roles in the ecosystems.

諏訪湖におけるユスリカに関する研究について,幼虫に関する知見を中心にこれまでの報告を整理した。宮地(1928)による報告以後,多くの研究者によって諏訪湖のユスリカについての種組成,季節消長,幼虫の分布,幼虫の浮泳活動,魚の餌としてのユスリカおよび物質代謝におけるユスリカの役飜に関する研究がなされてきた。これらの報告をもとにして,ユスリカ幼虫密度の変化と富栄養化の関係について考察を加えた。さらに,近年の諏訪湖のユスリカの生態および今後の研究課題について言及した。, Article, 信州大学山地水環境教育研究センター研究報告 1: 43-52(2003)

In order to better understand the characteristics of bronchial asthma caused by chironomid midges, cross-reactivity of midges was examined in 163 asthmatics through observation of skin reactions and specific IgE and histamine release. Positive skin reactions were observed in 17 of 73 asthmatics (23.3%) for Chironomus plumosus, 89 of 276 (32.2%) of those for Tokunagayusurika akamusi, and 33 of 128 (25.8%) of those for Chironomus yoshimatsui. Two cases of 32 (6.3%) asthmatics for Chironomus plumosus, 16 of 144 (11.1%) of those for Tokunagayusurika akamusi, and 7 of 77 (9.1%) of those for Chironomus yoshimatsui showed a significant amount of histamine release (more than 15%). Cross-reactivity was present for Chironomus plumosus, Tokunagayusurika akamusi, Chironomus yoshimatsui, by both skin tests and basophil histamine release. It is suggested that both the male and female of Chironomus plumosus have common antigenecity, and cross-reactivity was observed between them.