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Objective: To reveal the function of interferon gamma inducible protein 16 (IFI16) in multiple myeloma (MM) . Methods:Human multiple myeloma cells (RPMI-8266) were divided into Control group (untransfected cells), NC-sh group (cells transfected with negative control shRNA lentivirus), IFI16-sh group (cells transfected with IFI16 shRNA lentivirus), NC-OE group (cells transfected with negative control overexpressing lentivirus) and IFI16-OE group (cells transfected with IFI16 overexpression lentivirus) . Lipofectamine2000 was used for transfection and cells were collected after 48 h culture. Cell proliferation was detected by MTT assay and colony formation test. Apoptosis was detected by Annexin V-FITC and PI staining. Cell invasion was detected by Transwell. The m RNA levels of IFI16, Bcl-2, Bax, fibroblast growth factor (FGF) 1 and FGF2 were detected by qRT-PCR. The protein expression level of IFI16 was detected by Western blot. Nude mice were randomly divided into NC-sh group and IFI16-sh group (n=6) . Nude mice in NC-sh group and IFI16-sh group were subcutaneously injected with RPMI-8266 cell suspension transfected with NC-sh and IFI16-sh, respectively. After35 days, the tumor volume was measured and the tumor weight was weighed. The levels of Th1 cytokines [interferon-γ (IFN-γ), interleukin (IL) -12], Th2 cytokines (IL-4) and proinflammatory factors [IL-1β, IL-6, tumor necrosis factor-α (TNF-α) ] were detected by ELISA method. Results: Compared with Control group and NC-sh group, the relative expression of IFI16 m RNA and protein of RPMI-8266 cells in IFI16-sh group decreased (P＜0.05), relative cell viability and number of colony and invasive cells decreased (P＜0.05),the apoptosis rate and relative expression of Bax m RNA increased (P＜0.05), the relative m RNA expression level of Bcl-2, FGF1 and FGF2 decreased (P＜0.05) . Compared with Control group and NC-OE group, the relative expression of IFI16 m RNA and protein of RPMI-8266 cells in IFI16-OE group increased (P＜0.05), relative cell viability and number of colony invasive cells increased (P＜0.05), apoptosis rate and relative expression of Bax m RNA decreased (P＜0.05), the relative m RNA expression level of Bcl-2, FGF1 and FGF2 increased (P＜0.05) . Compared with NC-sh group, the tumor volume, tumor weight, the relative expression of IFI16 m RNA and protein in tumor tissue of IFI16-sh group decreased (P＜0.05), the levels of IFN-γ and IL-12 in peripheral blood increased (P＜0.05), the level of IL-4, IL-1β, IL-6 and TNF-α in peripheral blood decreased (P＜0.05) . Conclusion: IFI16 is a kind of oncogene in MM. Down-regulation of IFI16 can inhibit the growth and invasion of MM cells. The mechanism is related to correcting the balance of Th1/Th2 cells and inhibiting the activity of pro-inflammatory factors [ABSTRACT FROM AUTHOR]

Objective To evaluate the regulatory effect of the adaptor related protein complex 2 subunit μ1 (AP2M1) on proliferation and invasion of diffuse large B-cell lymphoma (DLBCL). Methods Human diffuse large B-cell lymphoma cell line OCI-LY8 was aliquoted into control group, NC-shRNA group, AP2M1-shRNA group, NC-LV group, and AP2M1-LV group. Lipofectamine 2000 was used for cell transfection. Cell proliferation was detected by tetramethylazolium salt (MTT) method, apoptosis was detected by flow cytometry and cell migration and invasion were detected by Transwell assay. The protein expression of AP2M1, epidermal growth factor receptor (EGFR), p-phosphatidylinositol 3 kinase (PI3K), PI3K, p-protein kinase B (Akt) and AKT was detected by Western blot. Results Compared with control group, the relative expression of AP2M1 mRNA and protein in the AP2M1-shRNA group was decreased (P＜0.05). The relative cell viability was increased (P＜0.05). The cell apoptosis rate was decreased (P＜0.05). The counting number of migrating and invading cells was increased (P＜0.05). The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were increased(P＜0.05). Compared with Control group, the expression of AP2M1 mRNA and protein relative expression level in AP2M1-LV group was increased (P＜0.05). The relative cell viability was decreased (P＜0.05). The cell apoptosis rate was increased (P＜0.05). The number of migrating and invading cells was decreased(P＜0.05). The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were all decreased (P＜0.05). Conclusions The over-expression of AP2M1 partially inhibits the proliferation and invasion of DLBCL cells by inhibiting the EGFR/PI3K/AKT signaling pathway. [ABSTRACT FROM AUTHOR]

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Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)