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Objective: To construct a prognostic risk model for hepatocellular carcinoma （HCC）， and elucidate the immune characteristics and immunotherapy response in patients with different prognostic stratification. Methods: RNA-seq data of TCGA-LIHC and ICGC（ LIRI-JP）， and gene microarray data of GSE14520 and GSE54236 in hepatocellular carcinoma， as well as clinical information of the corresponding samples were downloaded. First， screening of differentially expressed genes in tumor and non-tumor tissue samples from TCGA-LIHC， GSE14520 and GSE54236. For the common differential genes， univariate cox regression analysis was performed using TCGA-LIHC data to obtain HCC prognosis-related genes. Five genes were randomly selected as a panel， and the optimal prognostic marker panel was screened among 10 000 panels using Lasso-cox regression analysis combined with a five-fold cross-validation method. TCGA-LIHC data were used as training set to construct the prognostic risk model， and ICGC data were used as validation set to test the model performance. Tumor immune dysfunction and exclusion （TIDE） algorithm and Immunophenotypic score （IPS） were used to predict immunotherapy efficacy in patients in different prognostic groups. Results: Overall survival was significantly longer in low-risk group of HCC patients compared with high-risk group. Tumor proliferation rate， Treg and Th2 cell chemotaxis， stromal remodeling， and pro-tumor cytokines were significantly increased in high-risk patients， while NK cells， Th1 cells， effector cells and endothelial cells were significantly increased in low-risk patients. Immune checkpoint analysis showed that PDCD1， CTLA4 and CD276 were up-regulated in high-risk patients， while PDCD1LG2 was upregulated in low-risk patients. TIDE score and IPS results predicted that patients in low-risk group had better efficacy to immunotherapy. Conclusion: This study constructed a prognostic risk model containing three genes， DNASE1L3， RDH16 and DLGAP5， which can effectively predict the prognosis of HCC patients and assist in clinical decision making for individualized immunotherapy. [ABSTRACT FROM AUTHOR]

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The influence of different weighting agent on the performance of synthetic drilling fluid system (SBM) were studied in this work. Based on the MODRILL drilling fluid system of China Oilfield Services Co., LTD., the variation of SBM performance with 2.04 g/cm³ were investigated with different weighting agent of 4.3 g/cm³ Barite, 4.4 g/cm³ Barite and 4.8 g/cm³ Trimanganese tetra oxide with the ratio of 10∶0∶0、0∶10∶0、5∶0∶5、6∶0∶4、7∶0∶3、8∶0∶2、5∶5∶0. The results showed that the rheological property, emulsion-breaking voltage and high temperature and pressure filtration are affected by the different weighting agent. The apparent viscosity and the plastic viscosity would be decreased and the yield point and 6 rpm reading would be increased with the increasing trimanganese tetra oxide. The emulsion-breaking voltage would be decreased with the increasing trimanganese tetra oxide. The high temperature and high pressure filtration would be substantially increased when adding the increasing trimanganese tetra oxide. The above conclusions were verified in the filed test by studying the effect of weighting agent on the performance of synthetic drilling fluids. [ABSTRACT FROM AUTHOR]

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