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Objective To explore the effects of bioactive parts of Xiongma Decoction (parts of ethyl acetate and n-butanol extract) on the CGRP-CRLR/RAMP1 signal pathway so as to clarify its therapeutic mechanism on migraine. Methods We randomly divided 36 male SD rats into 6 groups with 6 in each: blank group, model group, groups of low-, medium- and high-dose Xiongma Decoction bioactive parts, and Sumatriptan group. By giving hypodermic injection of 10mg/kg nitroglycerin, the migraine rat model was copied; Only 18 rats were found to have positive expressions of CGRP, CRLR, and RAMP1 in TCC with immunohistochemistry staining after heart perfusion. For the remaining 18 rats, TCC was stripped directly from the whole brain and divided into two parts, one part used to detect CGRP, CRLR, RAMP1 mRNA expressions by qPCR, and the other part to detect CGRP, CRLR, RAMP1 protein expressions by Western blot. Results The number of CGRP, CRLR and RAMP1 immunoreactive cells, the mRNA and protein expressions on TCC in model group were effectively increased, compared with those in the blank group (P<0.05), indicating that the model copying was successful. Compared with those in the model group, the number of CGRP, CRLR and RAMP1 immunoreactive cells in Xiongma Decoction bioactive parts was significantly decreased, and the expressions of CGRP, CRLR and RAMP1 mRNA and protein were reduced (P<0.05). Conclusion The bioactive parts of Xiongma Decoction can reduce the activity of CGRP-CRLR/RAMP1 signal pathway in TCC of migraine rats. [ABSTRACT FROM AUTHOR]