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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Ischemic stroke is one of the main causes of death and long-term disability, and the incidence is still rising year by year, seriously affecting the quality of life. Blood-brain barrier injury is an important pathological process that leads to a poor prognosis of cerebral ischemia reperfusion injury. However, due to the limited treatment methods and limitations of multiple factors, it is urgent to find new treatment methods to repair blood-brain barrier injury. OBJECTIVE: To review the relevant mechanisms of traditional Chinese medicine combined with bone marrow mesenchymal stem cells in regulating the blood-brain barrier after cerebral ischemia reperfusion injury, to further explore new ways to protect the blood-brain barrier and provide assistance for clinical treatment. METHODS: The keywords were “traditional Chinese medicine, bone marrow mesenchymal stem cells, blood-brain barrier, cerebral ischemia reperfusion injury” in Chinese and English. WanFang, CNKI, PubMed, and Web of Science databases from 2012 to 2022 were retrieved for articles regarding the related mechanism of the blood-brain barrier damage and the related mechanism of Chinese medicine combined with bone marrow mesenchymal stem cells in regulating the blood-brain barrier. The obsolete and unqualified articles were excluded and 97 articles were included for analysis and summary. RESULTS AND CONCLUSION: (1) The physiological structure of the blood-brain barrier and the pathological changes of the blood-brain barrier structure after cerebral ischemia reperfusion injury were combed. (2) This paper summarized the mechanism of traditional Chinese medicine combined with bone marrow mesenchymal stem cells in regulating the blood-brain barrier after cerebral ischemia reperfusion injury, including regulating the expression of tight junction protein, inhibiting the activity of aquaporin-4 and matrix metalloproteinase-9, promoting angiogenesis, inhibiting the release of inflammatory mediators, and regulating exosomes. (3) It is concluded that traditional Chinese medicine combined with bone marrow mesenchymal stem cells can improve the permeability of the damaged blood-brain barrier and provide assistance for clinical treatment. [ABSTRACT FROM AUTHOR]

BACKGROUND: Rehmannia glutinosa has the effects of inhibiting oxidative stress, inhibiting apoptosis, inhibiting nerve inflammation, promoting angiogenesis and anti-aging, and has unique advantages in the treatment of ischemic stroke. The combination of Rehmannia glutinosa with mesenchymal stem cells as a carrier can play a better role in the treatment of ischemic stroke. OBJECTIVE: To review the research progress of Rehmannia glutinosa combined with mesenchymal stem cells in the treatment of ischemic stroke. METHODS: We searched CNKI and PubMed for the literature on the treatment of senile ischemic stroke with Rehmannia glutinosa combined with mesenchymal stem cells from 2012 to 2022. The keywords were “Rehmannia, mesenchymal stem cells (MSCs), ischemic stroke” in Chinese and English, respectively. Finally, 86 articles were included for further review. RESULTS AND CONCLUSION: (1) The mechanisms of mesenchymal stem cells in the treatment of ischemic stroke were reviewed, including the promotion of cell migration and differentiation, immunomodulation, and inhibition of apoptosis. (2) The mechanisms of Rehmannia glutinosa in treating ischemic stroke were summarized, including inhibition of oxidative stress, inhibition of nerve inflammation, inhibition of apoptosis, promotion of neuroangiogenesis, and anti-aging. (3) Rehmannia glutinosa could stimulate the vitality of mesenchymal stem cells, promote the proliferation of mesenchymal stem cells, induce the differentiation of mesenchymal stem cells into neuron-like cells, and improve the survival rate of mesenchymal stem cells in vivo, so as to achieve the goal of synergistic treatment of ischemic stroke. (4) The mechanism of Rehmannia glutinosa combined with mesenchymal stem cells in the treatment of ischemic stroke was summarized, including inhibition of oxidative stress, inhibition of apoptosis, anti-aging, promotion of angiogenesis and nerve remodeling, thereby providing theoretical reference for the new treatment of ischemic stroke in the future. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Osteoporosis is the property of Chinese Journal of Osteoporosis and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To study the effects of Zuoguiwan ( ZGW) on nourishing kidney essence, strengthening bone and marrow, and promotion of the proliferation of bone marrow mesenchymal stem cells ( BMSCs) and differentiation into osteoblasts. Methods The ZGW containing serum was prepared. 10% PBS DMEM group, osteogenic liquid group, 2. 5% serum of health rats with osteogenic liquid group, and 2. 5% serum containing ZGW osteogenic liquid group were set up. MTT method was used to observe the proliferation of BMSCs in each group. The differentiation of BMSCs into osteoblasts was tested using alkaline phosphatase calcium cobalt staining method . Alkaline phosphatase ( AKP) method was used to detect the content of AKP in cell culture medium. The expression of runt related transcription factor 2 ( Runx2 ), osteocalcin ( OPN), and alkaline phosphatase (ALP) protein in BMSCs were detected using Western blotting. Results Rapid proliferation of BMSCs was showed in 2. 5% serum containing ZGW osteogenic liquid group. The plateau stage appeared earlier. It promoted the secretion of AKP in BMSCs, increased the content of ALP, and up-regulated the protein expression levels of Runx2, ALP, and OPN in BMSCs cells. Conclusion The molecular mechanism of ZGW against osteoporosis may be related to the regulation of Runx2, activation of ALP and OPN protein expression, promotion of BMSCs proliferation and differentiation into osteoblasts. [ABSTRACT FROM AUTHOR]

Objective To explore the optimal conditions of cell counting kit-8 assay (CCK-8) and MTT assay on the detection of the proliferation of bone marrow mesenchymal stem cells (BMSCs) intervened by serum containing Zuoguiwan (ZGW). The advantages and disadvantages of the two method were compared. Methods BMSCs were intervened by serum containing ZGW. The optimal wavelength, culture time, sensitivity, and cell proliferation of BMSCs were detected by using CCK-8 and MTT method, respectively. Results Optimal detection wavelengths of CCK-8 and MTT reagents were 450 nm and 550 nm, respectively. The best incubation time of CCK-8 and MTT assay were 3 h and 4 h, respectively. Cell inoculation density should be respectively controlled by 0. 1x 10/ hole and 0. 25x 10/ hole, respectively. When the inoculation density of BMSCs was higher than 0.25x10*/well and 0. 5x10'/well, the OD values detected by CCK-8 assay and MTT assay entered the plateau stage earlier. It was easy to appear the false S-shaped growth curve. However, when the cell density was lower than 0.05x 10/well and 0. 1x 10/ well, it was difficult to form S-shaped growth curve because of slow growing, which could not reflect the proliferation characteristics of BMSCs. Compared with CCK-8 assay, there was no significant change in response to the increase of OD value of BMSCs in MTT assay, when BMSCs density was lower than 0. 1x 10/well or higher than 2x 10/well.. When BMSCs density was between 0.25x 10/well and 1x 10/well, the response of MTT assay to the elevation of OD value of BMSCs increased significantly (P< 0.05). Conclusion MTT assay is more suitable for the study of serum containing ZGW on the proliferation and differentiation of BMSCs. [ABSTRACT FROM AUTHOR]