【Objective 】High temperature resistance ability and heat acclimation ofmycelium were tested,in order to obtain high temperature resistant germplasm resources of Auricularia auricula-judae and provide breeding basis for cultivars of Auricularia suitable for cultivation in high temperature region in Guangxi.【Method 】15 strains ( including cultivar 916 ) of wide laria auricula-judae collected from Tianlin and Tiane county of Guangxi were studied by the following methods: Mycelium growth states in different temperature; Mycelium restorability testing after cultivated at 40 °C for different time ( 3, 5, 7, and 9 days) ; Mycelium growth states after heat acclimation (process: 40 °C for 5 days,28 °C for 7 days,42 °C for 3 days,28 °C for 7 days,45 Tifor3 hours,28 °C for 7 days )•【Result】 There were 6 strains [strain HME2,TY026,YE 1,TY041,YE2,YE(b) ] which can grow well at the temperature of 31 °C to 37 °C. After cultivated at 40 °C for different time, the mycelium restorability of 5 strains ( YE1, TY071 , TY041, TY026 and HME2 )were better than other strains. After the heat acclimation, there were 5 strains (TY041 ,TY026, TY071, HME2, TY073 )could grow well at 28 Tl 〇【Conclusion】 Strains TY026,TY041 and HME2 were identified as mycelia high temperature resistance varieties,Y071 and YE 1 have good heat resistance. [ABSTRACT FROM AUTHOR]