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Voltammetry is an important chapter and teaching difficulty in the course of Instrumental Analysis. However, the existing experiment teaching program has shortcomings in theoretical integration and application expansion. This paper introduces a cutting-edge research progress in material sciences, i.e., laser-scribed graphene technology, into voltammetry experiment teaching. The program enriches the experiment contents by introducing basic knowledge such as simulation of diffusion current theory and electrocatalytic phenomena, and extending applications such as vanillin detection in powdered milk. These new contents will help students to deeply understand the basic principles, research trends, and application value of the method. The comprehensive cost of the experiment is low, and the electrodes can be prepared in batches, thus making it suitable for experiment teaching. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Technology Economics is the property of Chinese Society of Technology Economics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Ecology & Rural Environment is the property of Journal of Ecology & Rural Environment Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Bioprocess Engineering is the property of Chinese Journal of Bioprocess Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor 165 (VEGF-165) are essential cytokines for bone repair. Lentiviral transfection of stem cells is important for studying the biological changes of doubly transfected cells. OBJECTIVE: To study the feasibility of human BMP-2 and human VEGF-165 dual gene transfection of bone marrow mesenchymal stem cells (BMSCs) and to explore the osteogenic capacity of doubly transfected cells. METHODS: Rabbit BMSCs were divided into four groups: untransfected group, blank vector group transfected with control lentivirus, BMP-2 group transfected with Lv-BMP-2/GFP, and BMP-2/VEGF-165 group transfected with Lv-BMP-2/GFP and Lv-VEGH-165/RFP. Expression of targeted proteins was detected using western blot at different time after transfection. MTT assay was used to detect cell proliferation in each group. Alkaline phosphatase activity was measured at 14 days after transfection. RESULTS AND CONCLUSION: (1) The corresponding green and red fluorescent protein expressions were observed under fluorescence microscope in the blank vector, BMP-2, and BMP-2/VEGF-165 groups. (2) The corresponding target proteins were highly expressed in the BMP-2 and BMP-2/VEGF-165 groups at 3 days after transfection, but no significant changes were detected at 7, 14, and 21 days after transfection (P > 0.05). (3) Compared with the BMP-2 group, the proliferation of cells was slightly faster in the BMP-2/VEGF-165 group (P < 0.05), but significantly lower in the untransfected group and blank vector group (P < 0.05). (4) The activity of alkaline phosphatase in the BMP-2, and BMP-2/VEGF-165 groups was significantly higher than that in the untransfected and blank vector groups (P < 0.05). These findings reveal that BMSCs were successfully transfected by hBMP-2 and hVEGF-165, and the dual gene transfection could promote the production of alkaline phosphatase, thereby accelerating the osteogenic differentiation of BMSCs. Subject headings: Bone Marrow; Mesenchymal Stem Cells; Bone Morphogenetic Proteins; Vascular Endothelial Growth Factors; Lentivirus Infections; Cell Proliferation; Tissue Engineering Funding: the National Natural Science Foundation of China, No. 31160199; the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2014GXNSFAA118263; the Scientific Research Project of Guilin Medical University, No. LX2014254 [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective · To investigate correlation of serum acute amyloid A (A-SAA) level and activity of Behcet's disease (BD). Methods · Blood samples from patients with active BD (n=40), remission BD (n=15), Takayasu disease (TA, n=12), rheumatoid arthritis (RA, n=25), systemic lupus erythematosus (SLE, n=25) and healthy donor (HD, n=25) were collected. Serum A-SAA, ESR, and CRP levels were detected and compared among groups. The correlation between A-SAA and its downstream IL-8 or International Society for Behcet's disease (ISBD) disease activity scores was analyzed as well. Results · Serum A-SAA level was significantly increased in the patients with active BD [(115.70±87.78) mg/L], as well as patients with RA and TA, which levels were (68.72±61.50) mg/L and (96.25±87.41) mg/L respectively, but remained unchanged in SLE patients. Moreover, serum A-SAA level was found correlated with ISBD score as well as IL-8 level. Conclusion · A-SAA can work as a detection index for evaluating BD activity. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Central China Normal University is the property of Huazhong Normal University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)