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Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] To identify samples of suspected tumor disease from a local chicken farm in Anhui Province. [Method] The chicken liver was collected and the virus DNA was extracted. The primers were designed according to the sequence of ALV and MDV that GenBank had registered. The ALV env and MDV meq gene were detected by PCR. The amplified sequence was sequenced and analyzed by evolutionary tree and nucleotide homology. [Result] Detected bands of ALV and MD, and sequence alignment results showed that ALV belongs to subgroup J, while MD is a strong wild virus strain. [Conclusion] This study provided reference data for understanding the mixed infection situation of ALV and MDV in the area. [ABSTRACT FROM AUTHOR]

In order to study the genetic and evolutionary characteristics of a novel goose astrovirus (N-GAstV), the full-length genes of Anhui strain AstV/AHHX were cloned in this study, and it was 7 251 nt. Sequencing analysis showed that AstV/AHHX had typical characteristics of N-GAstV. The phylogenetic tree showed that AstV/AHHX was relatively close to GDZJ37 isolates from China, G538 and G576 isolates from Shandong Province in recent years. The homology of ORF2 with GDZJ37, G538 and G576 was 100%, and the homology of ORF1a and ORF1b was more than 99.6%. Presumably they evolved from the same strain. Amino acid sequence analysis of ORF2 revealed 12 highly variable sites. They were the amino acid sites, 36 (Y/S/H), 60 (V/I), 224 (T/A), 228 (A/S/T), 229 (P/Q), 289 (T/A), 376 (T/A), 456 (D/E), 540 (Q/L), 608 (S/T), 610 (E/D/G), 614 (N/A/T). Amino acid mutations at position 224, 228, 229, 608 and 614 may lead to changes in the conformation and function of viral proteins, then the pathogenic ability of the virus was altered. [ABSTRACT FROM AUTHOR]

[Objective] Pseudo response regulators (PRRs) are important genes that regulate the flowering pathway in higher plants. The aim of this study is to clone the NtPRR37 gene from Nicotiana tabacum L. and analyze its response to different photoperiods and its effect on flowering, providing a target gene for tobacco flowering regulation. [Method] We cloned NtPRR37 gene from N. tabacum L. by homologous cloning method. Then we performed bioinformatics analysis, tissue-specific expression analysis, and expression pattern analysis of NtPRR37 gene under different light duration treatments. Concurrently, we used Virus Induced Gene Silence (VIGS) to reduce the expression of NtPRR37 and observed the phenotype and detected the expression changes of flowering-related genes. [Result] The fulllength CDS of NtPRR37 was 2 472 bp, encoded a peptide of 823 amino acids with a relative molecular weight of 90.16 kD. It contained REC and CCT domains, which were typical conserved domains of the PRRs gene family. Through homology evolution analysis, it was found that tobacco NtPRR37 belonged to the same branch as PRR37 from Nicotiana tomentosiformis, Nicotiana sylvestris, and Nicotiana benthamiana in evolution. The quantitative reverse transcription-PCR (RT-qPCR) analysis showed that, the expressions of NtPRR37 varied in various tissues of N. tabacum L. at full-bloom stage, with the highest expression in pistils and the lowest expression in lateral roots. Under different photoperiod treatments, the expression of NtPRR37 increased with the prolongation of light exposure. In different light duration treatments, the expression of NtPRR37 increased with the extension of light time, with the lowest expression under darkness, and demonstratted circadian rhythmicity. In NtPRR37-silenced plants, the expression of NtPRR37 was significantly downregulated, and the flowering period was advanced, which may be related to the significant upregulation of flowering-related genes (NtFT4, NtAP1, NtCO, NtSOC1). [Conclusion] The expression of NtPRR37 is regulated by the photoperiod, and NtPRR37 acts as a flowering repressor during the flowering process of tobacco. [ABSTRACT FROM AUTHOR]

Objective To analyze the molecular characteristics of the whole VP1 gene region of coxsackievirus A16 (CVA16) isolated from children with hand, foot and mouth disease (HFMD) in 2022 from a hospital in Kunming, Yunnan. Methods Two hundreds fecal specimens of HFMD clinical samples from 2022 in a hospital in kunming, were processed and isolated by RD cells and Vero cells, respectively, and viral nucleic acids were extracted from the cell cultures that still produced cytopathogenic effect (CPE) after three times of incubation, then amplified and sequenced by RT- PCR using universal primers 222/224, and sequenced, and the sequences were compared by BLAST to determine the serotypes, and then the full VP1 region of CVA16 was amplified with specific primers CA16VP1F/CA16VP1R, then sequenced and spliced. The standard sequences of CVA16 virus were downloaded, and the nucleotide and amino acid sequences of the whole VP1 region of CVA16 in this study were analyzed using software such as Geneious 5.4.1 and Mega 6.05. Results 22 strains of CVA16 isolated from Vero cells and 6 strains isolated from RD cells were detected by RT-PCR using universal primers, and the available sequences of 21 strains of full-length CVA16 VP1 region were further amplified by the specific primers of CVA16, and the nucleotide and amino acid sequences of the 21 strains of full-length CVA16 VP1 genes were highly homologous to each other, with 91.9%～99.6% and 89.2%～99.3%, respectively. The nucleotide and amino acid sequence homology with other strains of the chinese B1a genotype ranged from 89.7%～96.3% and 94.6%～99.0%, respectively; and nucleotide and amino acid sequence homology was in the range of 84.3%～87.2% and 89.2%～97.0%, respectively, when compared with the other reference strains of B1b. The phylogenetic analysis showed that all 21 CVA16 strains belonged to the B1a genotype. The 21 mutations were found by compared with that of other B1a strains. Conclusions The CVA16 strain that caused HFMD in a hospital in Kunming, Yunnan Province, China in 2022 belongs to the B1a genotype. Surveillance should be continued to clarify its epidemiological characteristics. [ABSTRACT FROM AUTHOR]

[Objective] Calcium-dependent protein kinases (CDPK or CPK) are widely involved in plant growth and development and environmental stress response. To explore their roles involved in Haematococcus pluvialis growth, development and astaxanthin accumulation, HpCDPK gene family was identified and analyzed. [Method] Bioinformatics tools were used to identify the HpCDPK gene family members, and to analyze the physical and chemical properties, phylogenetic evolution, conserved motifs and functional domains of the encoded proteins, as well as the gene expression profiles under nitrogen deficiency stress. [Result] The results showed that seven members of HpCDPK gene family were identified, and all HpCDPK proteins contained typical EF-hand motifs and protein kinase domains. Phylogenetic analysis showed that these HpCDPKs clustered with the CrCDPKs from Chlamydomonas reinhartii and separated from the AtCDPKs of Arabidopsis thaliana, suggesting that species-specific gene replication occurred in the process of evolution. Transcriptome data analysis showed that HpCDPK gene expression was induced by a variety of stresses, among which the transcription level of HpCDPK7 gene was most significantly up-regulated under nitrogen deficiency. In addition, the analysis of the correlation between HpCDPK and the expression of genes related to carotenoid synthesis showed that HpCDPK was closely related to the expressions of genes relevant to several carotenoid synthesis in H. pluvialis, especially HpCDPK2 was positively correlated with the expression of key genes of astaxanthin synthesis (BKT and BCH). [Conclusion] Seven HpCDPK genes were identified, the expression of HpCDPK7 gene was most significantly up-regulated under nitrogen deficiency and HpCDPK2 may play an active role in the synthesis and accumulation of carotenoids and astaxanthin. The results provide a reference basis for further analysis of the function and molecular mechanism of stress response and carotenoids synthesis and accumulation mediated by HpCDPK in H. pluvialis. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The study aimed to identify the viral pathogen of Capsicum annuum L. in Baise, Guangxi province and the results would provide theoretical basis for the effective prevention and control of the pepper viral disease. [Method] Four samples of C. annuum L. with leaf rolling and crinkling symptoms were collected from Baise, Guangxi province in 2022. The samples were identified and analyzed by polymerase chain reaction(PCR), gene cloning, nucleotide comparison, phylogentic tree construction. [Result] The PCR amplicons with expected size of 570 bp were obtained from 4 samples, the PCR products were sequenced and the sequences were analyzed in GenBank, the four sequences showed high nucleotide identities with tomato yellow leaf curl virus (TYLCV) and papaya leaf curl China virus (PaLCuCNV) isolates avilable in GenBank, respectively, and the results above confirmed that all of the four samples were infected by begomoviruses. To obtained the whole genome of the virus, two pairs of primers were designed based on the TYLCV (GenBank No. : MG904859 and KY783940) and PaLCuCNV (GenBank No. : KU892661 and MW779523) isolates available in GenBank. The PCR products were purified and cloned into the pMD18T vector, positive clones were selected for gene sequencing. Three whole genome sequences were obtained from two positive samples. Two of the three sequences had 91 % nucleotide identities with some of isolates of TYLCV available in GenBank, and one sequence had 91 % nucleotide identities with the some of isolates of PaLCuCNV available in GenBank. Following the guidelines of the classification of begomoviruses, the viruses infecting pepper in Guangxi were identified as TYLCV and PaLCuCNV. Phylogenetic tree analysis showed that the TYLCV Guangxi pepper isolate BS66-l branched with TYLCV SGl (Accession No. : MT969010, host: tomato) isolate, indicating their close evolutionary relationship; and BS67-1 branched with GX isolate and BS3 isolate (Accession No. : MW389934 and MT375610, host: tomato), indicating their close evolutionary relationship; The PaLCuCNV Guangxi pepper isolate BS66-2 clustered with PaLCuCNV GX0l (Accession No. : MW779523, host: tomato) isolate in a small branch, indicating their close evolutionary relationship. [Conclusion] TYLCV and PaLCuCNV are the pathogens that infect C. annuum (L.) in Guangxi. And this is the first report of the begomoviruses infecting C. annuum L. in Guangxi. [ABSTRACT FROM AUTHOR]

Copyright of Acta Prataculturae Sinica is the property of Acta Prataculturae Sinica Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In recent years, the medicinal and feeding value of Gelsemium elegans has become increasingly prominent. However, G. elegans is not tolerant to low temperatures during its growth process. Exploring its low temperature response genes may lay the foundation for the research of cold resistance breeding of G. elegans. Lipoxygenase（LOX）has a significant impact on the physiological and biochemical processes of plants in seed aging, stress resistance, and other aspects. The LOX genes of G. elegans in response to low temperature stress were mined from the transcriptome database of G. elegans constructed by our team. A full-length cDNA sequence of the GeLOX1 gene was cloned from the leaves of G. elegans using RT-PCR technology, and its bioinformatics, subcellular localization, gene expression, prokaryotic expression, and plate stress were analyzed. The results showed that the amino acid length of the protein encoded by the GeLOX1 was 761 aa, and the relative molecular weight of the protein was 87.00 kD. It was predicted to be an unstable hydrophilic protein, containing 28 serine phosphorylation sites, 22 threonine phosphorylation sites, and 9 tyrosine phosphorylation sites. The analysis results of evolutionary tree indicated that GeLOX1 belonged to the 9-LOX family. The subcellular localization test results showed that the GeLOX1 was located in the cytoplasm. Real time fluorescence quantitative PCR analysis revealed that the GeLOX1 was highly expressed in the roots of the G. elegans, and its expressions showed a downward trend under 4℃ low temperature stress. After prokaryotic expression induction, the recombinant protein of the GeLOX1 showed a target band at approximately 111 kD, and the accumulation of the recombinant protein reached its peak at 8 h of induction. In addition, the plate stress experiment showed that the prokaryotic expression strain of the GeLOX1 was more sensitive to low temperature stress compared to the control group. The research results indicate that GeLOX1 of G. elegans can respond to low temperature stress. [ABSTRACT FROM AUTHOR]

Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Society: Chinese Journal of Sociology / Shehui is the property of Society: Chinese Journal of Sociology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Alfalfa(Medicago sativa L.) contains various nutrients such as protein and vitamins, and is also an important forage widely planted worldwide. However, the infection of alfalfa leaf curl virus(ALCV) seriously affects the quality and yield of alfalfa. In this study, in order to provide some insights for antiviral breeding in the alfalfa industry, and also provide a theoretical basis for the prevention and control of alfalfa virus diseases. ALCV infection in alfalfa samples with leaf curling and shrinking symptoms in the Botanical Garden of Shanxi Agricultural University, Taigu district, Jinzhong city, Shanxi province was detected, by using the geminivirus universal primer(SPG1/SPG2), and the complete sequence of coat protein(CP) gene of ALCV-Taigu was further cloned and compared by PCR technology. The results showed that the nucleotide homology and amino acid homology of the ALCV-Taigu CP gene with other strains of ALCV were 82.7%–96.6% and 82.4%–97.1%, respectively. The phylogenetic analysis of the amino acid sequence of ALCV-Taigu CP showed that it was far from ALCV isolates from the European countries such as France, Spain, Italy, and the Middle Eastern countries such as Lebanon and Iran, and had a distant genetic relationship. ALCV-Taigu was relatively closed to ALCV isolates from China and Argentina, clustered into a large branch, and had a close genetic relationship. ALCV-Taigu isolate and Chinese alfalfa of ALCV-Helinger/N1 isolate were closely related and clustered together. This was the first time that ALCV had been detected on alfalfa in Shanxi. This study helped to gain a deeper understanding of the molecular evolution of ALCV. [ABSTRACT FROM AUTHOR]

Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Xinjiang Agricultural Sciences is the property of Xinjiang Agricultural Sciences Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective]To identify the suspected goose circovirus(GoCV) samples from a Landes geese farm in Lu’ an District,Anhui Province. [Method]The chicken liver was collected and the virus DNA was extracted. The primers were designed according to the sequence of GoCV that GenBank had registered. The GoCV was detected by PCR and the amplified sequence was sequenced. Finally, the obtained gene sequence was constructed and analyzed by evolutionary tree. [Result]The designed primers were used to detect the target gene band with a size of 580 bp. Sequence comparison results showed that the genetic distance between the identified strain and the Shandong strain KT387277. [Conclusion]This study provided reference for understanding the epidemic situation of GoCV in the area. [ABSTRACT FROM AUTHOR]

Copyright of Advanced Textile Technology is the property of Zhejiang Sci-Tech University Magazines and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Bioprocess Engineering is the property of Chinese Journal of Bioprocess Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Tea Science is the property of Journal of Tea Science Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To summarize the application of chromosome karyotype analysis combined with copy number variation sequencing (CNV-seq) in the fetuses with increased nuchal translucency (NT) . Methods: Karyotype analysis and CNV-seq were performed in 189 singleton fetuses with NT≥2.5 mm and gestation weeks from 11 to 13 +6, and the detection rate of chromosomal abnormalities was analyzed. According to the pre -interventional diagnosis indications, they were divided into the simple NT thickening group (n=119) and the non-simple NT thickening group (n=70). According to the NT thickness, they were divided into the 2.5 mm≤NT＜3.5 mm group (n=123) and the NT≥3.5 mm group (n=66). Results: Karyotype analysis revealed 31 cases (16.40%) of chromosome abnormalities, including 24 cases (12.70%) of aneuploidy and 7 cases (3.70%) of structural abnormalities, and CNV-seq revealed 38 cases (20.11%) of pathogenic copy number variations (pCNVs), including 24 cases (12.70%) of aneuploidy and 14 cases (7.41%) of other pCNVs. Nine additional cases (5.70%) of pCNVs were detected by CNV-seq in fetuses with normal karyotype. The detection rates of pCNVs and chromosome aneuploidy in the nonsimple NT thickening group were higher than those in the simple NT thickening group, and the differences were statistically significant (P＜0.05). There was no significant difference in the detection rates of other pCNVs (P＞0.05). The detection rates of pCNVs and chromosome aneuploidy in the NT≥3.5 mm group were higher than those in the 2.5 mm≤NT＜3.5 mm group, the differences were statistically significant (P＜0.05). There was no significant difference in the other pCNVs detection rates (P＞0.05). Conclusions: Fetal chromosomal abnormalities were increased with NT thickening or other pre-interventional diagnostic indicators, but they were not significantly associated with other pCNVs. CNV -seq combined karyotype analysis can help to detect the different types of chromosomal abnormalities, to avoid the missed diagnosis of chromosomal structural abnormalities and chromosomal microdeletion/ microduplication. [ABSTRACT FROM AUTHOR]

【Objective】 This study aimed to explore the IGF1R gene sequence structural characteristics and tissue expression in Kunming dogs, and to provide a basis for further study on the regulation mechanism of IGF1R gene in the growth and development and to lay a foundation for molecular genetics and breeding of Kunming dogs. 【Method】The IGF1R gene sequence of Kunming dogs was amplified and sequenced based on molecular cloning technology. Sequence homology alignment and phylogenetic tree of IGF1R gene were constructed by bioinformatics method. The physical and chemical property, sequence structural characteristics of the corresponding amino acid sequence were analyzed by online predicition software, and the tertiary structure model of IGF1R protein were predicted. Meanwhile, real-time quantitative PCR was used to detect the relative expression of IGF1R gene in various tissues of Kunming dogs. 【Result】The CDS region of IGF1R gene in Kunming dogs was 4104 bp encoding 1367 amino acids. IGF1R gene CDS in Kunming dogs had higher homology with Canis lupus familiaris(100%) and Vulpes vulpes(99.3%), but lower homology with Gallus gallus(78.8%). The molecular weight and theoretical isoelectric point of IGF1 R protein were 154 890.07 ku and 5.58, respectively.It contained three fibronectin type(FN3) domains, one furin like cysteine-rich(FU) domain and tyrosine kinase(TyrKc) domain, was a hydrophilic transmembrane protein. Subcellular localization analysis showed that IGF1 R protein was evenly distributed on endoplasmic reticulum, Golgi apparatus and plasma membrane, with the proportion of 33.3%, respectively. The analysis of phosphorylation sites showed that there were 123 phosphorylation sites in IGF1 R protein. The secondary structure of the protein was mainly composed of α-helix and random coil. Tissue expression showed that IGF1R gene was expressed in different tissues of Kunming dogs, especially in lung, heart and kidney. 【Conclusion】 IGF1R gene had strong conservatism in the process of biological evolution. IGF1 R protein of Kunming dogs had the characteristic domain of IGF receptor protein and belonged to hydrophilic transmembrane protein.IGF1 R gene was expressed in 6 tissues of Kunming dog, especially in heart, lung and kidney. [ABSTRACT FROM AUTHOR]

From 21 to mid-December, 2019, some red pandas(Ailurus fulgens)suspected to be infected with canine distemper virus(CDV)in a zoo park in Jiangsu, and tested weakly positive using canine distemper immunocolloidal gold test strips. 12 individuals were dead because of that. The gross lesions of the dead red panda appeared obvious pulmonary congestion, hepatomegaly, hemorrhage of lungs, endocardia and gastrointestinal mucosal. Blood samples from tail vein of two sick red pandas were tested and a RT-PCR test for canine distemper virus specific nucleic acid showed positive. Themonolayer of Vero-CD150 cells were inoculated to isolate the virus fromblood samples, and passaged serially on the cells, verified by RT-PCR, the test results of viral nucleic acid were still positive, and finally two strains of canine distemper were isolated. The viruses were referred to as SZ2019CDV1H and SZ2019CDV2H respectively. The H gene was amplified and sequenced, and the homology was compared with the related sequences in GenBank, then the variation of the H genes were determined. The results showed although two isolates have high homology(97. 2%), SZ2019CDV1H was belong to an African canine distemper strain, and SZ2019CDV2H was belong to an European wild animal canine distemper strain. The two isolates were apart from phylogenetic relationship. [ABSTRACT FROM AUTHOR]

In order to evaluate the reliability of molecular recognition features（MoRFs） prediction algorithms, in this paper, we propose a mathematical model to compare and evaluate the performance of MoRFs predictors （ANCHOR and MoRFCHiBi_web） based on randomly produced protein sequences, from the distribution of MoRFs residues, amino acid preference of prediction algorithms and prediction correlation between prediction algorithms. The results indicate that algorithms make different prediction in different degrees. Different MoRFs prediction algorithms adapt to different actual needs. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Dalian Ocean University is the property of Journal of Dalian Ocean University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The traditional high-throughput sequencing technology obtained after phenotyping analysis of tumor patients only represents the average phenotype of most cells, so a large amount of information will inevitably be lost. In order to make up for this shortcoming, single cell sequencing (SCS) technology arises at the right moment. As a revolutionary technology, it can analyze individual cells to gain insight into the differences in the microenvironment between tumor cells, and help the development of tumor targeted drugs. At present, this technology has been applied to a variety of lymphomas, including Hodgkin′s lymphoma and non-Hodgkin′s lymphoma, and shows great clinical application value. [ABSTRACT FROM AUTHOR]

During the wearing process, clothing will be contaminated by microorganisms from the human skin together with sebaceous lipids, sweat, and dead skin cells, which serve as a source of microbial nutrition. Therefore, clothing is easily contaminated by microorganisms in the air in the environment of daily life and is prone to mildew in warm and humid climate. The invasion of microbes in textile materials is mainly due to the presence of fungi. The growth of mildew on textiles has a series of effects not only on the textile itself but also on the wearer because mildew can secrete enzymes to decompose cotton, wool, and silk containing carbon, sulfur, and other nutrient sources into nutrients to promote their rapid reproduction and growth. At the same time, the mold will secrete organic acids and pigments during the growth process, causing acid corrosion and discoloration to the textiles, affecting their mechanical properties and aesthetics, and shortening the service life of the textiles. Therefore, it is necessary to identify the kinds of mildew on textiles, and adopt corresponding physical and chemical methods to inhibit their growth on the surface of the fabric according to the physiological and biochemical characteristics of these microorganisms, protect the textiles, and extend their service life. In this experiment, mildew microorganisms were extracted from moldy cotton fabrics. Then, they were separated and purified by streaking on the PDA culture medium suitable for mildew growth, and a single bacterial strain was obtained. Microbial morphology was used for preliminary identification, and the gene sequencing identification of mildew microorganisms was completed through ITS sequence analysis. The moldy cotton fabric was collected from the real environment, and the mold, causing microorganisms were isolated and purified from the above. The mildew was identified through microbial morphology and ITS sequence analysis, and the species of mildew and the dominant fungi causing the mildew of cotton fabric were determined, providing a scientific basis for preventing mildew and inhibiting the growth of mildew. Among the isolated and purified mildew, five moldy microorganisms were initially identified based on the morphological characteristics of a single colony, in which three (60% ) belonged to the genus Aspergillus, one belonged to the genus Penicillium, and one belonged to the genus Rhizopus. Aspergillus is the dominant genus of mildew,causing cotton fabrics. Five species were identified by sequence analysis and homology comparison, namely, Aspergillus chevalieri, Penicillium digitatum, Aspergillus sydowii, Rhizopus oryzae, and Aspergillus niger. Clarifying the types of microorganisms that cause mildew in textiles can help people to choose suitable storage environment and anti-mold finishing agent in accordance with their physiological and biochemical characteristics, so as to inhibit mildew growth and prolong the service life of textiles. [ABSTRACT FROM AUTHOR]

[Objective] STOP1 is an important gene for tolerance to aluminum (Al) -toxicity in plants. The purpose of the present study was to explore the relationship between the structural characteristics, expression level of STOP1 gene and its tolerance to Al in grape. [Method] Leaf cDNA of Vitis amurensis with weak tolerance to Al and V. sinocinerea with strong tolerance to Al were used as reference materials. Sequences of two genes VaSTOP1 and VsSTOP1 were obtained by PCR and TA cloning technology, and then bioinformatics analysis and expression analysis under Al stress were carried out. The membrane lipid peroxidation indices under Al stress were determined. Finally, the relationship between the expression of STOP1 genes, the changes of membrane lipid peroxidation of two species and tolerance to Al were compared. [Result] The sequences of VaSTOP1 and VsSTOP1 were 1782 and 1800 bp respectively, encoding 594 and 600 amino acids. Their relative molecular weights were 67. 03 and 67. 48 kD, and the theoretical isoelectric points were 5. 21and6. 42, respectively. The secondary structure in two species were the law of irregular curl > α-helix > folding extension chain, and the tertiary spatial structure were composed of zinc protein, α-helix, β-folded sheet and β-rotation angle. The phylogenetic tree analysis found that the homology between VaSTOP1 and VsSTOP1 was as high as 96%, and the homology between them and CcSTOP1, PnSTOP1, NtSTOP1 and ErSTOP1 was more than 80%. The expression of VaSTOP1 gene increased first and then decreased within 28 days under Al stress, but the peak value appeared earlier, whereas the VsSTOP1 gene expression increased steadily, which was basically consistent with the change law of membrane lipid peroxidation. [Conclusion] The tolerance to Al of Vitis was positively correlated with the expression of STOP1 gene, which provided a reference for further exploring the molecular regulation mechanism of Vitis tolerance to Al. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] Copper zinc superoxide dismutase (Cu/Zn-SOD) played an important role in protecting cells and tissues from oxidative damage. In the present study, Cu/ Zn-SOD were cloned and sequence analyzed from salt-stressed Scaevola sericea. The findings will provide a scientific basis for further revealing the adaptation mechanism of S. sericea to the saline-alkali environment. [Method] The cDNA sequence of Cu/ Zn-SOD was obtained by RACE, named as SsCSD. The bioinformatics analysis was performed to research the SsCSD, and the recombinant plasmid pBinGlyRed-SsCSD was constructed. [Result] The full length of SsCSD was 1148 bp and contained an open reading frame of 687 bp, which encoded 288 amino acid residues. Amino acid sequence analysis revealed that there was a typical Cu/Zn-SOD functional domain, belonging to SOD family. The bioinformatics analysis showed that it was located in the chloroplast. Meanwhile, evolution and clustering analysis showed that the Cu/Zn-SOD protein of S. sericea was highly homologous with known plant of Cu/Zn-SOD protein in chloroplasts. Besides, the recombinant plasmid pBinGlyRed-SsCSD was constructed and transferred to Agrobacterium tumefaciens GV3101. [ Conclusion] We obtained the full length of SsCSD, which the amino acid sequence was highly similar with the known species and highly conserved in evolution, which was of great significance to further reveal the biological function of the Cu/Zn-SOD in S. sencea. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The present study aimed to reveal the relationship between the amino acid sequence, secondary structure, tertiary structure and their functions of the three keratinases produced by the Streptomyces sp. DJ strain. [Method] Based on whole genome sequencing results of DJ strain, the sequence and structure of its three keratinases were analyzed and predicted by using software, such as Bio-edit, DNAMAN, Discovery Studio2.5 et al, and website like https://swissmodel.expasy.org/,and so on. [Result] DJ strains secreted three kinds of keratinases（K1,K2 and K3）,and the keratinases belonged to serine proteases of the S8 family, and their signal peptides, leading peptides and mature peptides were more consistent in the composition, polarity and length of amino acid residues. Three keratinases were modeled using 5 WSL.1.A as template by the homology modeling technique. The 3-D structure of each keratinase contained six α-helix, two 310-helix and fifteen β-folded secondary structures.K1 contained two disulfide bonds, 1 Ca and 2 Ca sites, while K2 and K3 had only one disulfide bond and 1 Ca site. The Pro270 residues of all three keratinases were respectively located in the loop ring, while only Pro242 residue of K2 was also located in the loop ring. The substrates binding regions of the three keratinases were mainly composed of hydrophobic residues, so these keratinases preferred substrate with strong hydrophobicity. For the substrate binding regions of the keratinases, the S1 and S2 played a key role in substrate specificity. And the substrate specificity was different due to the non-conservative nature of the residues at important sites in the S1 and S2 of the three keratinases. [Conclusion] The differences in amino acid, disulfide bond, proline and calcium binding sites leaded to the different stability of the three keratinases, besides the surface charge distribution and substrate binding regions resulted the complementarity to the feather hydrolysis sites. This might be an important reason for the efficient degradation of feathers by DJ strains. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Hydrobiology / Shuisheng Shengwu Xuebao is the property of Editorial Department of Journal of Hydrobiology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Due to persistent systemic inflammation and immunosuppressive conditions, patients with liver cirrhosis are susceptible to bacterial infection, which may progress to sepsis without proper control. Early effective antibiotic therapy is the key to improving the prognosis of patients with sepsis. This article briefly describes the pathophysiological mechanism of sepsis in patients with liver cirrhosis and the current status of the diagnosis and treatment of sepsis, and it is pointed out that metagenomic next-generation sequencing can be used to guide effective antibacterial treatment of sepsis. [ABSTRACT FROM AUTHOR]

Copyright of Journal of South China Agricultural University is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)