Kunming-Montreal Global Biodiversity Framework sets out key actions for biodiversity conservation worldwide in the coming period, including integrating biodiversity and its multiple values into economic and social activities. As a party to the United Nations Convention on Biological Diversity (CBD), China has made unremitting efforts to promote the biodiversity mainstreaming, integrating biodiversity conservation into top-level national decision-making and major strategic planning, and integrating it into the policies, norms and assessment mechanisms of ecological environments, natural resources and other relevant industries. Different functional departments, scientific research institutions, enterprises, social organizations and other organizations have carried out extensive and in-depth researches based on multiple aspects of biodiversity conservation and management. Benchmark the objectives of Kunming-Montreal Global Biodiversity Framework and refer the advanced experience of biodiversity mainstreaming practices in other countries, we discussed and analyzed the conceptual connotation of biodiversity mainstreaming, summarized the practice and stage results of biodiversity mainstreaming in China. Then focusing on different actors including governments, enterprises and the public, we propose the implementation path of promoting biodiversity mainstreaming in an all-round way for China in the new era, including: (1) To import a consistent action framework which is mitigation protection hierarchy with four steps, contains avoidance, mitigation, recovery, and offset; (2) To give play to the leading role of government governance, and to integrate biodiversity conservation into government governance systems and industrial development layout with the help of various planning and policy tools; (3) To support enterprises to take joint actions and internalize ecological and environmental hazards into business operations which can promote sustainable production and consumption; (4) To raise public awareness to promote broad participation in biodiversity conservation, establish a system of public participation in biodiversity conservation through various publicity and education actions, to guide the public to practice a green and low-carbon lifestyle, and to translate into real benefits of biodiversity and climate mitigation and improvement. By taking the above actions, we will strive to integrate biodiversity into the policy mechanisms of government departments at all levels and social production and life practices, so as to provide references for improving biodiversity governance decisions. [ABSTRACT FROM AUTHOR]