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为明确元宝枫种仁的营养成分，对元宝枫种仁含油率、粗蛋白质含量，元宝枫种仁粕中氨基酸组成及含量、矿质元素组成及元宝枫种仁油的总脂肪酸、Sn-2位脂肪酸组成及含量、生育酚含量进行测定。结果表明：元宝枫种仁的含油率为43.7%，粗蛋白质含量为35.13%；元宝枫种仁粕中含有17种氨基酸，其中7种人体必需氨基酸占氨基酸总量的33.78%，是理想的蛋白质资源；元宝枫种仁粕中含有丰富的矿质元素，常量元素中K含量为6 372.8 μg/g，Na含量仅为346.5 μg/g，属于高钾低钠食品，微量元素中Mn含量较高，为68.1 μg/g，且未检出重金属元素；元宝枫种仁油主要由12种脂肪酸组成，神经酸含量达到5.89%，以亚油酸和α-亚麻酸为主的多不饱和脂肪酸主要分布在Sn-2位；元宝枫种仁油中生育酚总量为1 230.70 mg/kg，以γ-生育酚含量最高，达到792.62 mg/kg。元宝枫种仁营养丰富，可作为神经酸、生育酚和氨基酸的重要来源，其油脂可作为富含神经酸的保健食用油。 To clarify the nutritional components of Acer truncatum seed kernel, the contents of oil and crude protein in Acer truncatum seed kernel, the amino acid composition and mineral element composition of Acer truncatum seed kernel meal, the composition of total fatty acids, Sn-2 fatty acids and tocopherol content in Acer truncatum seed kernel oil were analyzed. The results showed that the contents of oil and crude protein in Acer truncatum seed kernel were 43.7% and 35.13% respectively. Acer truncatum seed kernel meal contained 17 kinds of amino acids, of which 7 kinds of essential amino acids accounted for 33.78%, and it was an ideal protein resource. Acer truncatum seed kernel meal was rich in mineral elements, with K content in major elements of 6 372.8 μg/g, and Na content of 346.5 μg/g, belonging to high potassium and low sodium food. The Mn content in trace elements was higher(68.1 μg/g), and no heavy metal element was detected. Acer truncatum seed kernel oil was mainly composed of 12 kinds of fatty acids, and the content of nervonic acid reached 5.89%. Polyunsaturated fatty acids, mainly linoleic acid and α-linolenic acid, were mainly distributed in Sn-2 position. The total contents of tocopherols in Acer truncatum seed kernel oil was 1 230.70 mg/kg, in which γ- tocopherol content was the highest, reaching 792.62 mg/kg. Acer truncatum seed kernel is rich in nutrition and can be used as an important source of nervonic acid, tocopherol and amino acids. Acer truncatum seed kernel oil can be used as a health edible oil.

为了优化透明光网络中IRWA（基于物理损伤模型的路由波长分配）算法性能,通过分析多种物理损伤特点,构建了一种可替代经典模型的简化损伤模型,在此基础上提出一种基于RSVP-TE（资源预留协议-流量工程）的有向泛洪IRWA算法,即KDF-IRWA算法,该算法令源节点通过多条不同的备选路径向目的节点发送PATH（路径）消息包,这些PATH消息包各自独立收集所选路径中各个链路的路径信息,并在目的节点汇聚、计算和比较,避免了经典算法中因首次建路失败而引起的重发包,提高了算法的效率。仿真结果表明,该算法能够有效降低算法时间,提高建路成功的概率,降低网络阻塞率。

Plant nursery presents the characteristics of close planting. It is difficult for the traditional air-assisted sprayer to take into account the dual requirements of "long spray range and uniform droplet deposition", which leads to the problems of low deposition and drift.A goose-neck long-range air-assisted sprayer for nursery was designed based on CFD simulation and free submerged jet theory. The three-outlet flow guide device, as the core component of the sprayer, has outlets A, B, and C. Air outlet A adopts a circular arc shape, while air outlet B and air outlet C are gradually expanding circular arc tubes. The three air outlets are interconnected and correspond to different areas, which can meet the requirements of far-range spray operation. The optimized curvature radius parameters are conducive to maintaining a uniform and stable flow state of airflow. Based on the free submergence jet theory, the air outlet and fan parameters were determined, and airflow field distribution measurement experiments were conducted. when the fan speed is no less that 2 200 r/min, the airflow speed can fit the spraying requirement as far as 20 m. The average airflow speed was 2.33 m/s at a height of 0.8 m ; the airflow speed was 1.74 m/s at 20.0 m. The variation coefficient of airflow speed at different heights was less than 30%, indicating that the energy attenuation rate was slow and uniform, and the airflow could carry droplets to spray far away at a uniform speed. The droplet deposition test shows that the variation coefficient of the droplet deposition amount of the goose-neck long-range nursery air spray is 28.87%. To ensure the reliability of our findings,a comprehensive field test of the sprayer were conducted. Citrus seedlings, with an average height of 80 cm, were chosen as the test subjects, and the test was carried out in the nursery of the Citrus Research Institute of Chongqing Southwest University. Following the guidelines of GB/T17997-2008, the droplet deposition effect and drift rate at various distances were tested .The experimental results show that the fan speed of the sprayer is 2 400 r/min and the driving speed is 1 m/s; the droplet deposition tends to be less-more-less with the increase of distance. The droplet coverage rates of the upper and lower leaves of the canopy were 51.93% and 24.38%, respectively, and 42.99% and 19.26%, respectively. The average droplet deposition on the front and back leaves of the upper canopy was 13.34 and 2.82 μl/cm², respectively, and that on the lower canopy was 5.44 and 2.63 μl/cm², respectively, which met the requirements of plant protection spraying operation. It can be seen that the sprayer shows good spray range performance and droplet deposition uniformity, which can meet the agronomic requirements of the complex planting environment in the nursery and the industrial standards of the air-assisted sprayer. At present, in Chinese nurseries such as citrus, pear, and apple, etc, manual machinery accounts for a large proportion of spraying equipment, and the demand for specialized sprayers is becoming increasingly urgent. Due to the close planting mode of the nursery, it can be considered that the two modes of spray with high clearance spraying and long-range airflow- assisted spraying have application prospects. Compared with high-clearance spraying, long-range airflow-assisted spraying has the characteristics of high operating efficiency and good horticultural trafficability and is more suitable for small and medium- sized nurseries. However, improving the delivery distance of aerosol flow while ensuring good uniformity of droplet deposition is still a challenge. The results of this study can provide a new idea for developing a long-range air-assisted sprayer for nurseries. [ABSTRACT FROM AUTHOR]

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Objective To investigate the effect of Traditional Chinese Medicine (TCM) five-element cupping therapy on postoperative wound pain of patients undergoing Milligan-Morgan surgery for mixed hemorrhoids of damp-heat type. Methods Totally 60 patients undergoing Milligan-Morgan surgery for mixed hemorrhoids of damp-heat type were randomly divided into the observation group and control group, with 30 cases in each group. Anti-infection, hemostasis and other symptomatic treatment were carried out after surgery. The control group received routine wound cleansing and dressing change, and the observation group received TCM five-element cupping therapy on the basis of routine care. The Visual Analogue Scale (VAS) was used to assess the wound pain degree at 24 h、48 h、72 h after treatment. Results The VAS score in the treatment group was significantly lower than that in the control group at 24 h、48 h and 72 h after treatment, respectively (P＜0. 01). Conclusion The TCM five-element cupping therapy is effective to relieve the postoperative pain of patients with mixed hemorrhoids. It is an easy-to-operate and safe technique of TCM nursing, which is worthy of promoting in pain control after surgical treatment for anorectal diseases. (目的 观察五行能量罐治疗混合痔(湿热下注型)术后伤口疼痛的效果。方法 选取2021年5月—12月行手术治疗的混合痔(湿热下注型)患者共60例, 采用随机数字表法分为观察组和对照组, 各30例。两组患者均在麻醉下行混合痔外剥内扎术, 术后常规给予抗感染、止血等对症治疗。对照组接受术后常规换药护理, 观察组在对照组基础上增加五行能量罐中医护理干预。采用疼痛视觉模拟评分(VAS)分别对治疗后24 h、48 h、72 h伤口疼痛程度进行评价。结果 观察组拔罐治疗后24 h、48 h、72 h的疼痛评分低于对照组, 差异有统计学意义(P＜0. 01)。结论 五行能量罐中医护理干预能减轻混合痔术后患者伤口疼痛程度, 且操作简便, 患者依从性好, 可以广泛应用于包含混合痔在内的多种肛肠疾病术后疼痛护理中。)

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Background and purpose: There is a consensus within the medical community regarding the perioperative treatment of locally advanced gastric cancer (LAGC), yet the selection of an efficient and safe treatment strategy remains a contentious issue. Previous studies have validated the effectiveness of chemotherapy in conjunction with immune checkpoint inhibitor (ICI) and antiangiogenic agents in the perioperative treatment of LAGC. This study aimed to investigate the perioperative treatment regimen combining ICI, anti-angiogenic drugs and chemotherapy, in order to provide additional references for the selection of perioperative treatment strategies for patients with LAGC. Methods: This was a prospective, single-arm exploratory study designed to assess the efficacy and safety of perioperative treatment with penpulimab combined with anlotinib and chemotherapy for potentially resectable stage T3-4N+M0 LAGC. Patients diagnosed with LAGC at the Inner Mongolia Autonomous Region People’s Hospital from January 2022 to December 2023 were enrolled in the study. The primary endpoints were pathological complete response (pCR) rate and major pathological remission (MPR) rate, while secondary endpoints included surgical conversion rate, objective response rate (ORR), disease-free survival (DFS), progression-free survival (PFS) and adverse reactions. Quality of life before and after treatment was evaluated using the European Organization for Research on Treatment of Cancer (EORTC) QLQ-STO22 Chinese version questionnaire. This study was reviewed and approved by the Ethics Committee of Inner Mongolia Autonomous Region People’s Hospital (number: 202404604L). Results: A total of 32 patients participated in the study, with an ORR of 78.1% (25/32). Of these, 18 patients underwent surgical treatment, achieving a pCR rate of 22.2% (4/18) and a MPR rate of 38.9% (7/18). The median PFS for patients who did not undergo surgery was 9.8 months (95% CI: 7.6-13.1), and the median follow-up duration was 16.4 months (95% CI: 11.7-22.5). For patients who received radical surgical resection, the median DFS was not reached, and the 1-year DFS rate was 88.8% (16/18). Common adverse reactions following the treatment included myelosuppression, liver function abnormalities, diarrhea and neurotoxicity, with most adverse events being grade 1-2, a few grade 3 adverse events, and no grade 4 or 5 adverse events. According to the EORTC QLQ-STO22 Chinese version questionnaire, significant improvements were observed in the domains of difficulty swallowing, pain and eating restrictions. Additionally, improvements were noted in anxiety and reflux symptoms, while changes in body image were less pronounced. Conclusion: In the perioperative treatment of LAGC, the combination therapy of oxaliplatin, tegafur, anlotinib and penpulimab can improve the efficacy while ensuring safety, and is expected to become a new treatment for LAGC. [ABSTRACT FROM AUTHOR]

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Interleukin-35 (IL-35) is an important immunosuppressive cytokine that has been shown to play a role in the immune response of various diseases. In this study, we cloned the coding sequence of human IL-35 gene, constructed single subunit expression vectors pXC17.4-p35 and pcDNA3.1 (+)-EBI3, and co-transfected CHO-K1 cells to express IL-35 in vitro. No binding was found between subunits of p35 and EBI3. Knobs-into-Holes (KIH) can solve the problem of heavy chain mismatch of heterologous antibodies. Therefore, expression vectors pXC17. 4-p35-Fch and pcDNA3.1(+)-EBI3-Fck were constructed by fusing KIH structures on the basis of the original sequences to express the recombinant fusion protein of KIH-IL-35. The expression vectors of two subunits were exchanged at the same time to verify the influence of different vectors on the expression level of KIH-IL-35. The analysis of various protein detection methods showed that the correct expression rate of KIH-IL-35 structure was significantly improved. Affinity purification of KIH-IL-35 was performed after large amount of expression, and the binding activity of KIH-IL-35 to glycoprotein 130 (gp130) was detected by ELISA. The results showed that the binding of KIH-IL-35 to gp130 was concentration dependent. The indirect activity of KIH-IL-35 and M1 cells was detected by cell activity assay. Further results showed that the inhibition rate of M1 cells increased in a dose-dependent manner with the concentration of KIH-IL-35. In addition, a method for determining IL-35 activity by activated human peripheral blood mononuclear cells was successfully established. Activated PBMCs increased in a dose-dependent manner with KIH-IL-35 concentration. In summary, this study utilized the KIH-IL-35 model to enhance the expression of recombinant human IL-35 and validated its high activity in vitro, providing new ideas for the study of IL-35 and the recombinant expression of similar heterodimeric cytokines. [ABSTRACT FROM AUTHOR]

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Knowledge tracing aims to model the state of knowledge and ultimately predict the future performance of learners by describing exercises through the representation of concepts. However, in terms of the representation of concepts, the current research doesn’t model the influence of historical knowledge concepts on the temporal relationship of the current concepts, nor does it describe the role of the spatial relationship between various concepts in the exercise. In order to solve these problems, this paper proposed a knowledge tracing model characterized by temporal and spatial correlation fusion. First of all, based on the degree of temporal correlation between concepts, it modelled the temporal effect of historical concepts from current concepts. Secondly, it modelled the spatial interaction between several concepts contained in the exercise to obtain the representation of knowledge points containing temporal and spatial information through the graph attention network. Finally, it used the above representation of concepts to derive the representation of the exercises, and generated the current state of knowledge through the self-attention mechanism. In the experimental stage, this paper compared the performance of the proposed model with the five relevant knowledge tracing models on four real datasets. The results show that the proposed model has better performance. In particular, compared to the five comparative models on the ASSISTments2017 dataset, the AUC and ACC are improved by 1.7%～7.7% and 7.3%～12.1%, respectively. At the same time, the ablation experiment proves the effectiveness of modeling the temporal and spatial correlation between concepts, and the training process experiment shows that the proposed model has certain advantages in the representation of concepts and the modeling of their interaction relationships. The application examples can also show that the model has better practical results than other knowledge tracing models. [ABSTRACT FROM AUTHOR]

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