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为挖掘黑果枸杞籽油的潜在开发利用价值，采用石油醚浸提法提取黑果枸杞籽油，对黑果枸杞籽油的理化性质进行测定，分析其脂肪酸组成及含量，并测定其对DPPH·、ABTS+·的清除能力和总还原能力。结果表明：黑果枸杞籽油碘值（I）为(84.0±0.2) g/100 g，皂化值（KOH）为(130.1±0.3) mg/g，过氧化值为(0.42±0.03) g/100 g，酸值（KOH）为(8.4 ±0.2) mg/g，需进一步精炼；黑果枸杞籽油主要包括亚油酸（68.31%）、油酸（17.76%）、棕榈酸（5.37%）、γ-亚麻酸（3.73%）、硬脂酸（2.58%）等9种脂肪酸；黑果枸杞籽油对DPPH·清除率的IC50为0.587 mg/mL，3.2 mg/mL黑果枸杞籽油的ABTS+·清除率为57.50%，且其具备一定的Fe3+还原能力。因此，黑果枸杞籽油富含不饱和脂肪酸，体外抗氧化活性良好，具有开发的价值和潜力。 In order to tap the potential development and utilization value of Lycium ruthenicum seed oil, Lycium ruthenicum seed oil was extracted by petroleum ether, and its physicochemical properties, fatty acid composition and content , DPPH· and ABTS+· scavenging power, and total reducing power were analyzed . The results showed that the iodine value, saponification value, peroxide value and acid value of Lycium ruthenicum seed oil were (84.0±0.2)gI/100 g, (130.1±0.3)mgKOH/g, (0.42±0.03) g/100 g, and (8.4±0.2) mgKOH/g, respectively, and it needed to be further refined. Lycium ruthenicum seed oil contained 9 kinds of fatty acids, such as linoleic acid (68.31%), oleic acid (17.76%), palmitic acid (5.37%), γ-linolenic acid (3.73%), and stearic acid (2.58%) and so on. The IC50 of Lycium ruthenicum seed oil for DPPH·scavenging rate was 0.587 mg/mL, the ABTS+· scavenging rate of 3.2 mg/mL Lycium ruthenicum seed oil was 57.50%，and it had a certain Fe3+ reduction power. Lycium ruthenicum seed oil is rich in unsaturated fatty acids, and it has good in vitro antioxidant activity, so it has development value and potential.

Objective To analyze and validate the potential molecular mechanisms involved in the formation of vulnerable atherosclerosis plaques through the miRNA mRNA regulatory network. Methods Morphological staining was used to validate the presence of atherosclerotic plaques in the aortic root and brachiocephalic trunk. Differentially ex- pressed miRNAs (DEMs) from the GSE34646 and GSE34647 datasets were analyzed, and their target genes (DEMS TGs) were predicted. Differentially expressed genes in advanced plaque (DEGSAP) were then screened from the GSE10000 dataset. The intersection of DEGSAP and DEMsTGs was taken to identify candidate genes. A protein protein interaction (PPI) network was constructed for the identified genes, and key genes were identified. An miRNA mRNA regulatory network was established, and the prognostic value of key genes was assessed using receiver operating characteristic (ROC) curves. Finally, RTqPCR was used to detect the predicted mRNA and miRNA levels in the aorta. Results Histopathological examination showed significant vulnerable plaques in the model group. Forty-four DEMs were identified in vulnerable plaques from the GSE34646 and GSE34647 datasets, predicting 3,171 and 8,075 miRNA target genes, respectively. The GSE10000 dataset identified 3,441 DEGsAP. The intersection of DEGS - AP and DEMS - TGS yielded 936 candidate genes. The PPI network identified 10 upregulated and 10 downregulated hub genes, which were used to construct the miRNA mRNA regulatory network. Conclusion Let-7g-3p-ADAM10/PIK3R1/C3AR1 may be the potential miRNA-mRNA regulatory axes for prognostication and diagnosis of advanced plaques by the RTqPCR. [ABSTRACT FROM AUTHOR]

N6 -methyladenosine (m6 A) modification is one of the most abundant epitranscriptomic modifications in eukaryotic mRNA, with dynamic and reversible properties. This modification process is coordinated by methyltransferases, demethylases, and related m6 A binding proteins, which in turn affect mRNA metabolism and function. Increasing evidence has indicated that the m6 A RNA modification plays an important role in the occurrence and development of atherosclerosis (As) and other related diseases. This paper provide a comprehensive review of the relationship between m6 A RNA modification and As. The entire manuscript summarizes the m6 A RNA modification mechanism and its roles in Asrelated cells including endothelial cells, macrophages, and smooth muscle cells, and discusses the association of m6 A RNA modification with risk factors of As such as high-fat diet, ischemia/hypoxia, oscillatory stress, and hypertension. Finally, this review summarizes researches on drug intervention targeting m6 A RNA methylation to mitigate As. These studies provide important references for exploring new targets for early diagnosis and treatment of As. [ABSTRACT FROM AUTHOR]

Objective To compare the preparation efficiency of mouse pox and mouse hepatitis antibodies between two substrains of BALB/c and Big-BALB/c (B-BALB/c) mice, and to provide a theoretical basis and reference for the selection of laboratory animals in the preparation of monoclonal antibodies induced in vivo through hybridoma. Methods Individuals weighing more than 5% of the weight of normal animals at 4 weeks of age (the criterion for late selection is more than 10%) were selected from a population of conventionally bred BALB/c mice and bred individually, and a subline of B-BALB/c mice was prepared after 10 generations of selection. A total of 40 BALB/c mice and 40 B-BALB/c mice aged 10 to 11 weeks, half male and half female, were selected and inoculated with the mousepox monoclonal antibody hybridoma cell line G23 or the murine hepatitis monoclonal antibody hybridoma cell line Y15 pre-treated with liquid paraffin, respectively. Mice ascites containing monoclonal antibodies were obtained by in vivo induction. The antibody titer was tested by indirect ELISA. The mice were grouped based on the sub-strains, gender and inoculation type of hybridoma to analyze the ascites production, antibody titer and antibody production, and to evaluate the antibody preparation efficiency of the two BALB/c mouse sub-strains. Results After 10 generations of breeding, the body weight of 10-week-old male and female B-BALB/c mice increased by 22.3% and 12.8%, respectively, compared with BALB/c mice of the same age. Compared with BALB/c mice, B-BALB/c mice had better tolerance and adaptation to secondary ascites collection. Compared with BALB/c mice, the ascites production and antibody titer during the preparation of antibodies in B-BALB/c mice were significantly increased, especially in the hybridoma cell line G23 vaccination group (both P＜0.000 1) . After inoculation with the hybridoma cell lines G23 or Y15, the average antibody production of B-BALB/c mice (14.99×104 U and 33.22×104 U) was higher than that of BALB/c mice (5.33×104 U and 19.31×104 U) (both P＜0.01). After inoculation with hybridoma cell line G23, the average antibody production per unit body weight of B-BALB/c mice (0.55×104 U/g) was higher than that of BALB/c mice (0.23×104 U/g) (P＜0.000 1). And the antibody production per unit body weight of female B-BALB/c or BALB/c mice was higher than that of male B-BALB/c or BALB/c mice (both P＜0.01). Conclusion B-BALB/c mice can be used as an alternative to BALB/c mice in the in vivo induction of monoclonal antibody preparation, which can achieve the purpose of reducing the number of experimental animals used, lowering the labor cost, and improving the efficiency of antibody preparation. [ABSTRACT FROM AUTHOR]

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Objective To observe the expression changes of DNA methyltransferase 3a ( DNMT3a), tissue plasminogen activator ( tPA) and apoptosis induced by oxygen glucose deprivation and reoxygenation ( OGD/R) in rat cortical astrocytes, as well as the intervention of S-adenosyl methionine ( SAM) . Methods Primary cultured cortical astrocytes were isolated and identified, and then were divided into the control group, OGD/R group, and OGD/R+SAM group. Cells in the control group were routinely cultured. In the OGD/R group and OGD/R+SAM group, the OGD/R cell injury models were established ( oxygen glucose deprivation 3 h, reoxygenation 24 h), and the cells in the OGD/R+SAM group were treated with 50 μmol/L SAM during the modeling process. DNMT activity was measured by DNA methylation assay; DNMT3a, tPA, Bcl-2, Bax, and Cleaved Caspase-3 protein expression levels were determined by Western blotting; and RT-PCR was used to detect the expression of DNMT3a and tPA mRNA expression level after OGD 3 h and reoxygenation and glucose 24 h. Results DNMT activity and DNMT3a mRNA and protein expression in the OGD/R group were lower than those in the control group, but tPA mRNA and protein expression levels were higher than those in the control group ( all P＜0.05) ; DNMT activity and DNMT3a mRNA expression in the OGD/R+SAM group were higher than those in the OGD/R group, and the expression levels of tPA mRNA and protein were lower than those in the OGD/R group ( all P＜0.05) . The Bcl-2 protein expression and the Bcl-2/Bax ratio of the OGD/R group were lower than those of the control group, and the Bax and Cleaved Caspase-3 protein expression levels were higher than those of the control group ( all P＜0.05) ; the Bcl-2 protein expression and Bcl-2/Bax ratio in the OGD/R+SAM group were higher than those in the OGD/R group, and the Bax and Cleaved Caspase-3 protein expression levels were lower than those in the OGD/R group ( all P ＜ 0. 05) . Conclusion OGD/R decreases DNA methylation, increases tPA expression, and induces apoptosis of astrocytes; SAM can reverse these changes. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect of semi rigid ureteroscopy, flexible ureteroscopy and Sun’s endoscopy with ureteroscopy in the treatment of upper ureteral calculi, and provide reference for the choice of clinical treatment. Methods: The clinical data of 515 cases in the Department of Urology of our hospital from August 2015 to December 2017 with ureteroscopic holmium laser treatment of upper ureteral calculi were retrospective analyzed. They were divided into three groups according to the type of ureteroscopy, there were 297 cases in hard mirror group(treated with semi rigid ureteroscopy), 172 cases in soft mirror group(treated with flexible ureteroscopy), and 46 cases in Sun’s mirror group(treated with Sun’s endoscopy with ureteroscopy). The operation time,first stage stone clearance rate, postoperative hospitalization time, intraoperative and postoperative complications were compared between the three groups. Results: The stone clearing rate in hard mirror group, soft mirror group and Sun’s mirror group were 81.5%, 94.2% and95.7% respectively, and stone clearing rate in the soft mirror group and Sun’s mirror group were significantly higher than that in the hard mirror group(P<0.05). The incidence of intraoperative complications in the hard mirror group, soft mirror group and Sun’s mirror group were 18.9%, 5.8% and 4.3% respectively. The incidence of intraoperative complications in the hard mirror group were significantly higher than that in the soft mirror group and Sun’s mirror group(P<0.05). There was no significant difference in the incidence of postoperative complications between the three groups(P>0.05). The average operation time in the hard mirror group,soft mirror group and Sun’s mirror group were(28.6±6.2)min,(49.4±12.4)min,(6.1±6.5)min respectively. The average operation time in the soft mirror group were significantly higher than that in the hard mirror group and Sun’s mirror group, the differences were statistically significant(all P<0.05). The average hospitalization time in the hard mirror group, soft mirror group and Sun’s mirror group were(5.4±3.2)d,(6.7±5.7)d,(5.0±2.5)d respectively. The time of hospitalization in the soft mirror group were significantly higher than that in the hard mirror group and Sun’s mirror group, the differences were statistically significant(all P<0.05). Conclusion: The effect of clearing stone and the safety of operation of flexible ureteroscopy is more advantageous than rigid ureteroscopy, but the operation time and the postoperative hospitalization time is long. Sun’s endoscopy with ureteroscopy is expected to be a better choice for the treatment of upper ureteral calculi. [ABSTRACT FROM AUTHOR]