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To analyze the relationship between coagulation and fibrinolysis indicators and acute traumatic coagulopathy (ATC) after traumatic brain injury (TBI) and their predictive efficacy for cerebrocardiac syndrome ( CCS ). 80 patients withTBI admitted to our hospital from January 2020 to December 2022 were selected as the research objects. The levels of prothrombin time(PT), activated partial thromboplastin time (APTT), D-dimer (D-D), thrombin-antithrombin complex (TAT), plasminogen-α2 plasmino-gen inhibitor complex (PIC), international normalized ratio (INR) and TAT / PIC ratio were detected and calculated in non-ATC patientsand ATC patients, non-CCS patients and CCS patients, respectively. Bivariate Spearman correlation was used to test the correlation between coagulation and fibrinolysis indicators. Multivariate Logistic model was established to analyze the influencing factors of ATC andTBI combined with CCS after TBI, and the predictive efficacy of coagulation and fibrinolysis indicators on TBI combined with CCS wasanalyzed. The incidence of ATC in 80 TBI patients was 27. 50 %. Compared with the non-ATC group, the levels of PT, APTTand D-D in the ATC group were higher, and the TAT / PIC ratio was lower (P<0. 05). PT, APTT and D-D were positively correlated withATC after craniocerebral injury, and TAT / PIC was negatively correlated with ATC. Multivariate logistic analysis showed that TAT /PIC was an independent risk factor for ATC after craniocerebral injury (P<0. 05). The incidence of CCS in 80 TBI patients was 20. 00 %. Compared with the non-CCS group, the levels of PT, APTT and D-D in the CCS group were higher, and the TAT / PIC ratio was lower(P<0. 05). Multivariate logistic analysis showed that PT, APTT, D-D and TAT / PIC were independent risk factors for TBI combined with CCS (P<0. 05). In the prediction of TBI combined with CCS, the ACU of PT, APTT and D-D were all > 0. 70, and TAT / PIC > 0. 85. TAT / PIC is associated with ATC after TBI, and can also predict the occurrence of CCS, which has guiding significance in the prognosis prediction of TBI patients. [ABSTRACT FROM AUTHOR]

Objective: To explore the relationship between serum C-reactive protein (CRP), albumin, S100B protein and the severity of traumatic brain injury and its complication with acute traumatic coagulation disease. Methods: 80 patients with brain injury admitted to our hospital from March 2020 to March 2023 were selected as the research subjects. All patients were evaluated using the Glass Coma Score (GCS), and the severity of the brain injury was determined based on the score results. 25 patients with a GCS score of ≤ 8 were divided into a severe group, and 55 patients with a GCS score of>8 were divided into a non severe group. Compare the expression levels of serum CRP, albumin, and S100B protein between two groups of patients. Subsequently, 80 patients with traumatic brain injury were divided into an acute traumatic coagulation disease group (n=20) and a non acute traumatic coagulation disease group (n=60) based on whether they were complicated with acute traumatic coagulation disease. The general clinical situation of the two groups of patients was compared, and the predictive value of serum CRP, albumin, and S100B protein for the occurrence of acute traumatic coagulation disease in patients with traumatic brain injury was analyzed. Results: There was no difference in albumin levels among patients with different severity of brain injury(P>0.05), but there was a difference in CRP and S100B protein levels. The CRP level in the severe group was higher than that in the non severe group, while the S100B protein level was lower than that in the non severe group (P<0.05); The Spearman correlation analysis results showed that there was no significant correlation between albumin and the severity of brain injury (P>0.05), CRP was positively correlated with the severity of brain injury, and S100B protein was negatively correlated with the severity of brain injury (P<0.05); There was no significant difference in gender, age, BMI, and type of brain injury between the acute traumatic coagulation disease group and the non acute traumatic coagulation disease group(P>0.05). However, there was a significant difference in the range of brain contusion, treatment time, GCS score, shock index, CRP, albumin, and S100B protein levels between the acute traumatic coagulation disease group and the non acute traumatic coagulation disease group(P<0.05); The results of logistic regression analysis showed that GCS score, shock index, CRP, and albumin were independent influencing factors for acute traumatic coagulation disease after brain injury(P<0.05). Conclusion: The detection of CRP and S100B protein on admission can assist the severity of craniocerebral injury early, and when patients with high CRP level and low S100B protein and albumin level, they should be alert to the occurrence of acute traumatic coagulopathy, and take timely measures to intervene for patients. [ABSTRACT FROM AUTHOR]

To identify highly effective antiviral compounds and develop green antiviral agents for plants, 21 α-hydroxy-γ-butyrolactone derivatives were designed and synthesized. Their structures were characterized using ¹H NMR, 13C NMR and HRMS. The in vivo antiviral activities against tobacco mosaic virus (TMV) were evaluated in three modes (inactivation, curative, and protective). Bioassay results revealed that many of the target compounds exhibited potent anti-TMV activities. Notably, compound 8o displayed prominent inactivation activity with an EC50 value of 226.2 μg/mL, which was significantly superior to ribavirin (EC50 = 308.4 μg/mL). [ABSTRACT FROM AUTHOR]

Scientific post-evaluation for initial water rights allocation is great significant to give full play to the key regulatory role of the market in the efficient utilization and optimal allocation of water resources, and to promote water rights trading. Aiming at the objective balance of quantitative weighting of index system and the correlation between certainty and uncertainty in the post-evaluation of regional initial water rights allocation effect, this study adopted principal component analysis (PCA) to weight the index system, and established a PCA-SPA coupling post-evaluation method of regional initial water rights allocation effect based on set pair analysis (SPA) theory. The proposed method was applied to post-evaluation of regional initial water rights allocation effect in Binzhou City. The results show that the initial water rights distribution effect of Zouping City, Wudi County and Boxing County is “excellent”, the initial water rights distribution effect of Huimin County is “good” and the trend of upgrading from “good” to “excellent” is strong, and the initial water rights distribution effect of Bincheng District, Zhanhua District and Yangxin County is “average”. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of chloroquine (CQ) on proliferation, apoptosis and autophagy of human cervical cancer cell line HeLa and to preliminarily explore its mechanism of action. Methods HeLa cells were divided into control group and experimental groups: chloroquine-1 to chloroquine-5 groups (25,50,75,100, 150 μmol/L CQ respectively). The cells all were cultured for 24 and 48 h; CCK-8 and colony formation assays were used to detect cell proliferation; reactive oxygen species detection kit was used to detect intracellular ROS production; flow cytometry was used to detect apoptosis rate. The expression level of autophagy proteins p62, LC3 and Beclin1, apoptosis proteins Bax, Bcl-2 and PARP and PI3K/AKT/MDM2 pathway proteins was detected by protein immunoblotting. Results CQ inhibited cell proliferation in a time- and concentration-dependent manner (P＜0.01); CQ significantly inhibited cell autophagy, induced intracellular ROS production and induced apoptosis (P＜0.01). Compared with control group, CQ significantly inhibited the activation of PI3K/AKT/MDM2 signaling pathway (P＜0.05). Conclusions Chloroquine may inhibit the proliferation of HeLa cells and induce apoptosis through the PI3K/AKT/MDM2 pathway. [ABSTRACT FROM AUTHOR]

Objective: To investigate the changes of serum tumor necrosis factor-α(TNF-α), C-reactive protein(CRP) and procalcitonin(PCT) in children with different hand foot and mouth disease(HFMD) of different conditions, and to analyze the predictive value of the combination of the three detection for poor prognosis. Methods: 117 children with HFMD(HFMD group) who were admitted to the pediatric department of our hospital from January 2019 to January 2022 were selected, and they were divided into 32 cases in the general group, 45 cases in the severe group and 40 cases in the critical group according to different conditions, and they were divided into 32cases in the poor prognosis group and 85 cases in the good prognosis group according to the prognosis after treatment, and another 50healthy children were selected as the control group. The basic data of children with HFMD were collected, and serum TNF-α, CRP and PCT levels were measured in all study subjects. Multivariate Logistic regression was used to analyze the influencing factors of poor prognosis in children with HFMD, and receiver operating characteristic(ROC) curve was used to analyze the predictive value of serum TNF-α, CRP and PCT levels on poor prognosis in children with HFMD. Results: The serum TNF-α, CRP and PCT levels in the HFMD group were higher than those in the control group(all P<0.05). The serum TNF-α, CRP and PCT levels in the general group, severe group and critical group were increased successively(all P<0.05). The results of univariate analysis showed that the proportion of fever≥39℃, fever duration≥3 d, disease grading as critical disease, proportion of neutrophil, TNF-α, CRP and PCT levels in the poor prognosis group were higher than those in the good prognosis group, while the proportion of lymphocytes, cardiac output and oxygenation index were lower than those in the good prognosis group(all P<0.05). Multivariate Logistic regression analysis showed that fever duration≥3 d,disease grading as critical and increased TNF-α, CRP and PCT were independent risk factors for poor prognosis in children with HFMD,while increased cardiac output and oxygenation index were independent protective factors(all P<0.05). ROC curve analysis showed that the area under curve of serum TNF-α, CRP and PCT alone and combined to predict poor prognosis in children with HFMD were 0.769,0.793, 0.799 and 0.947, respectively. The area under curve of serum TNF-α, CRP and PCT combined to predict poor prognosis was greater than that of each index alone. Conclusion: The increase serum TNF-α, CRP and PCT levels are associated with the exacerbation of the disease and poor prognosis in children with HFMD. The combined detection of serum TNF-α, CRP and PCT levels has a high predictive value for poor prognosis in children with HFMD. [ABSTRACT FROM AUTHOR]

The study was to improve the supply chain management level of feed enterprises and promote the modernization of feed industry supply chain. Based on the perspective of Chinese modernization, based on three transformation directions of feed enterprise supply chain management technology digitization, planning intensification and organization coordination, the supply chain management mode of feed enterprise was constructed from the aspects of demand management, relationship management, order management and service feedback management, and the specific implementation path was put forward. The results showed that the constructed supply chain management mode features data collaboration, precise management and emphasis on experience, which was helpful to optimize the management system of feed enterprises and improve the overall operation efficiency of the supply chain. The research shows that the supply chain management model of feed enterprises has management flexibility, which has a certain value of promotion and practice. [ABSTRACT FROM AUTHOR]

Pre-eclampsia is one of the common complications of pregnancy and is an important cause of maternal and perinatal mortality. The disordered functions of placental trophoblast cells are closely associated with the development of preeclampsia. Inadequate invasion of placental extravillous trophoblast cells and abnormal spiral artery remodelling are dominant pathologic mechanisms in pre-eclampsia. The PI3K/Akt (phosphatdylinositol-3 kinase/protein kinase B) signalling pathway plays a significant role in regulating proliferation, survival, apoptosis, migration, and invasion of trophoblast cells. MicroRNAs (miRNAs), long non-coding RNAs and circular RNAs belonging to non-coding RNAs (ncRNA) have multi-level regulatory roles on genes and influence the functions of trophoblast cells by regulating the PI3K/Akt signalling pathway, thus contributing to the occurrence and development of pre -eclampsia. This review introduces the recent advances of ncRNAs related to PI3K/Akt signalling pathway in pre -eclampsia in an effort to provide a reference for exploring and discovering key molecules in the pathogenesis of pre-eclampsia. [ABSTRACT FROM AUTHOR]

Salient object detection (SOD ) aims at detecting the significant parts in the images automatically and efficiently, which plays an important role in computer vision tasks such as object detection and recognition, key points location, visual tracking, semantic segmentation and so on. This paper focused on a comprehensive review of salient object detection, which covered the closely related fields, models, datasets, applications and future directions. It introduced the relationship between salient object detection and closely fields at first. Then, it analyzed salient object detection algorithms in terms of flowcharts, ideas, innovations, performance as well as applicability. Thirdly, it introduced the public datasets which improving continuously with the development of the saliency detection models. Finally, it discussed a prospect of future researches and several real application scenes of salient object detection. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship of expression of androgen receptor（AR), matrix metalloproteinase 9（MMP-9), E-cadhenrin with clinicopathological characteristics and prognosis of triple negative breast cancer（TNBC） tissue. Methods:Cancerous tissue and paracancerous tissue which were resected surgically in 91 patients with TNBC and 61 cases of breast hyperplasia（control group） paraffin specimens which were collected in Shuyang Hospital Affiliated to Xuzhou Medical University from January2014 to January 2017 were selected, expression of AR, MMP-9, E-cadhenrin was measured by immunohistochemical method. The relationship between the expression of AR, MMP-9, E-cadhenrin and the clinicopathological parameters of TNBC was analyzed, the relationship between the expression of AR, MMP-9, E-cadhenrin and the prognosis of patients with TNBC was analyzed by Kaplan Meier survival curve, Cox risk ratio regression. Results: MMP-9 positive expression rate in TNBC cancerous tissue were higher than paracancerous tissue tissues and control group, AR, E-cadhenrin positive expression rate were lower than paracancerous tissues and control group（P<0.05). Expression of AR was correlated with degree of differentiation, histological grade, lymph node status, expression of MMP-9 was correlated with histological grade, lymph node status, Ki-67 expression, and expression of E-cadhenrin was correlated with histological grade, lymph node status（P<0.05). Kaplan-Meier survival analysis showed that the survival rate of patients with positive expression of MMP-9 was lower than those of patients with negative expression of MMP-9, and the survival rate of patients with negative expression of AR, E-cadhenrin was lower than that of patients with positive expression of AR, E-cadhenrin（P<0.05). COX regression analysis showed that lymph node status N1-2, positive expression of MMP-9, AR, E-cadhenrin negative expression were risk factors for poor prognosis of TNBC patients（P<0.05). Conclusion: Excessive expression of MMP-9, lack of AR, E-cadhenrin expression were associated with malignant invasion behavior of tumor and poor prognosis in TNBC patients, and evaluation of expression of AR, MMP-9,E-cadhenrin could provide certain basis for prognosis prediction in TNBC patients. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship of expression of androgen receptor(AR), matrix metalloproteinase 9(MMP-9), E-cadhenrin with clinicopathological characteristics and prognosis of triple negative breast cancer(TNBC) tissue. Methods:Cancerous tissue and paracancerous tissue which were resected surgically in 91 patients with TNBC and 61 cases of breast hyperplasia(control group) paraffin specimens which were collected in Shuyang Hospital Affiliated to Xuzhou Medical University from January2014 to January 2017 were selected, expression of AR, MMP-9, E-cadhenrin was measured by immunohistochemical method. The relationship between the expression of AR, MMP-9, E-cadhenrin and the clinicopathological parameters of TNBC was analyzed, the relationship between the expression of AR, MMP-9, E-cadhenrin and the prognosis of patients with TNBC was analyzed by Kaplan Meier survival curve, Cox risk ratio regression. Results: MMP-9 positive expression rate in TNBC cancerous tissue were higher than paracancerous tissue tissues and control group, AR, E-cadhenrin positive expression rate were lower than paracancerous tissues and control group(P<0.05). Expression of AR was correlated with degree of differentiation, histological grade, lymph node status, expression of MMP-9 was correlated with histological grade, lymph node status, Ki-67 expression, and expression of E-cadhenrin was correlated with histological grade, lymph node status(P<0.05). Kaplan-Meier survival analysis showed that the survival rate of patients with positive expression of MMP-9 was lower than those of patients with negative expression of MMP-9, and the survival rate of patients with negative expression of AR, E-cadhenrin was lower than that of patients with positive expression of AR, E-cadhenrin(P<0.05). COX regression analysis showed that lymph node status N1-2, positive expression of MMP-9, AR, E-cadhenrin negative expression were risk factors for poor prognosis of TNBC patients(P<0.05). Conclusion: Excessive expression of MMP-9, lack of AR, E-cadhenrin expression were associated with malignant invasion behavior of tumor and poor prognosis in TNBC patients, and evaluation of expression of AR, MMP-9,E-cadhenrin could provide certain basis for prognosis prediction in TNBC patients. [ABSTRACT FROM AUTHOR]

Objective: To analyze the correlation between intraoperative hemorrhage and anesthesia factors during the repeat cesarean section to provide research basis for optimizing anesthesia management program. Methods: A total of 1192 parturient women, who underwent caesarean section again, were selected and divided into study group (the amount of intraoperative hemorrhage≥ 500 mL) and control group (the amount of intraoperative hemorrhage <500 mL) according to the amount of intraoperative hemorrhage. The general factors and anesthesia factors related to intraoperative hemorrhage between the two groups of parturient women were compared and analyzed. Results: The amount of intraoperative hemorrhage of 106 cases ≥500 mL, the incidence was 8.89%. The proportion of abortion history, the proportion of prenatal anemia, the proportion of placenta previa, the proportion of placenta adhesion, the proportion of placenta implantation, the proportion of uterine atony of the parturient women in the study group were higher than those in the control group, and the pregnancy process was shorter than that in the control group, the differences were statistically significant (P<0.05). The time of operation, the proportion of hysterectomy or partial hysterectomy, the proportion of ASA Ⅱ ~ Ⅲ grade and the proportion of ropivacaine dosage > 15 mg of the parturient women in the study group were significantly higher than those in the control group, the differences were statistically significant (P<0.05). The results of logistic multiple regression analysis showed that the occurrence of intraoperative hemorrhage during the repeat cesarean section was correlated with placenta previa, placenta implantation, uterine atony, hysterectomy or partial resection and ASA grade (P<0.05). Conclusion: The risk of intraoperative hemorrhage during the repeat cesarean section is not only related to placental and uterine factors, but also affected by operation and anesthesia factors. The clinician should make a comprehensive and accurate preoperative evaluation on the parturient women, optimize anesthesia management plan and operation strategy, so as to reduce the occurrence of intraoperative hemorrhage and ensure the safety of operation. [ABSTRACT FROM AUTHOR]

Objective: To compare the cleaning effect of the new rotating instruments mani GPR and ProTaper retreatment system and hand H file in the removal of curved endodontic gum in root canal retreatment. Methods: Thirty simulated canals with a 30-degree curvature in resin blocks were instrumented up to #30/.06 with ProTaper Next NiTi rotary instruments and obturated using gutta percha and AH plus root canal sealer. The specimens were randomly divided into three groups (n=10 each). Removal of gutta-percha was performed with the following devices and techniques: Group 1 (H-files), Group 2 (ProTaper Universal Retreatment), Group 3 (Mani GPR). The retreatment time was recorded for each specimen using a stopwatch. After radiographing in buccolingual and mesiodistal directions, the amount of remaining gutta-percha in the roots was quantified using Image J 2X software. Apically extruded debris were weighted using balances. Statistical analysis was performed with one-way ANOVA test. Results: The volume of remaining filling material was significantly less in H-files and Mani GPR groups than in ProTaper Universal Retreatment group (P<0.05). The total retreatment time was significantly shorter in the ProTaper Universal Retreatment and Mani GPR groups compared with the manual group (P<0.05). Mani GPR files were associated with significantly less extruded debris than with the ProTaper Universal Retreatment (P<0.05). Conclusion: Mani GPR files left less gutta-percha and sealer than ProTaper Universal Retreatment and H-files. The NiTi rotary systems were significantly faster than the manual group in the time required for gutta-percha removal. [ABSTRACT FROM AUTHOR]

Abstract：Objective To investigate the effects of Yiqi Gubiao pills on visceral fat accumulation, white fat browning and metabolic inflammation in chronic obstructive pulmonary disease (COPD) mice, and to explore the possible mechanism. Methods Thirty C57BL/6 male mice were randomly divided into the control group, COPD group and Yiqi Gubiao pills group, with 10 mice in each group. Mice in the COPD group and Yiqi Gubiao pills group were treated with inhalation of cigarette smoke exposure combined with intraperitoneal injection of cigarette smoke extract to establish COPD models. Yiqi Gubiao pills group was given 200 µL of Yiqi Gubiao pills solution once a day for 2 weeks after modeling. Mice in the control group and COPD group were given equal volume of normal saline through intragastric administration. Lee's index and lipid-body ratio of each group were measured. Lung tissues and white and brown adipose tissues were taken after death for pathological observation. Western blotting was used to detect the protein expression levels of browning-related indicators of white adipose tissues ［silent information regulator 1 (SIRT1), peroxisome proliferator-activated receptors γ (PPARγ), uncoupling protein 1 (UCP1), peroxisome proliferator activated receptor γ coactivator-1α (PGC-1α), PR domain-containing 16 (PRDM16) ］ and inflammatory response-related indicators ［tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6) and and IL-8］. UCP1 relative fluorescence intensity in white and brown adipose tissues was detected by immunofluorescence method, the mRNA expression levels of TNF-α, IL-6 and IL-8 in white adipose tissues was detected by real-time fluorescence quantitative PCR method, and serum levels of IL-6, IL-8 and TNF-α were detected by ELISA. Results In the COPD group, the pulmonary alveolar wall was slightly thickened, the alveolar interval was widened and inflammatory cells infiltrated. Compared with the COPD group, the above-mentioned manifestations of pulmonary tissues of Yiqi Gubiao pills group were reduced. Compared with the control group, the volume of adipose cells in the white and brown adipose tissue of the COPD group increased, and the vacuoles in the cells increased. Compared with the COPD group, the fat droplets in white and brown adipose tissues of the Yiqi Gubiao pills group decreased, showing browning. Compared with the blank control group, Lee's index, lipid-body ratio and serum levels of IL-6, IL-8 and TNF- α increased, the relative expression levels of TNF-α, IL-6 and IL-8 protein and mRNA in the white adipose tissues increased, the relative expression levels of SIRT1, PPARγ, UCP1, PGC-1α and PRDM16 proteins in white adipose tissue and the relative fluorescence intensity of UCP1 in white and brown adipose tissues decreased in the COPD group (all P<0. 05) . Compared with the COPD group, Lee's index, lipid-body ratio and serum levels of IL-6, IL-8 and TNF-α decreased, and the relative expression levels of TNF-α, IL-6 and IL-8 protein and mRNA in white adipose tissue decreased, the relative expression levels of SIRT1, PPARγ, UCP1, PGC-1α and PRDM16 proteins in white adipose tissues and the relative fluorescence intensity of UCP1 in white and brown adipose tissues increased in Yiqi Gubiao pills group (all P<0. 05) . Conclusion Yiqi Gubiao pills can reduce visceral fat accumulation and metabolic inflammatory response in COPD mice, and the mechanism may be related to promoting the browning of white fat by targeting SIRT1/PPARγ/UCP1 pathway. [ABSTRACT FROM AUTHOR]

Objective: To investigate the changes of prothrombin time (PT), neutrophil/lymphocyte ratio (NLR), and procalcitonin/albumin (PCT/ALB) in sepsis patients with different etiological results of bloodstream infection (BSI) and their relationship with prognosis. Methods: 130 sepsis patients with BSI admitted to the Shanghai Fifth People's Hospital Affiliated to Fudan University from January 2021 to March 2023 were selected, and they were divided into fungal group with 11 cases, gram-positive bacteria (G+ bacteria) group with 49 cases and gram-negative bacteria (G-bacteria) group with 70 cases according to different etiological results. Sepsis patients with BSI were divided into death group with 36 cases and survival group with 94 cases according to the 28d clinical outcome. The PT, NLR, PCT/ALB were detected and calculated. Multivariate Logistic regression was used to analyze the factors affecting the prognosis of sepsis patients with BSI, and receiver operating characteristics (ROC) curve was used to analyze the predictive value of PT, NLR, PCT/ALB in predicting death in sepsis patients with BSI. Results: The PT of G-bacteria group was longer than that of fungal group and G+ bacteria group, and the NLR and PCT/ALB were higher than those of fungal group and G+ bacteria group (P<0.05). There were no differences in PT, NLR and PCT/ALB between fungal group and G+ bacteria group (P>0.05). Multivariate Logistic regression analysis showed that septic shock, PT prolongation, Sequential Organ failure assessment (SOFA) score elevated, Acute physiological and Chronic Health Assessment Ⅱ (APACHE Ⅱ) score elevated, NLR elevated, PCT/ALB elevated were independent risk factors for the prognosis of sepsis patients with BSI (P<0.05). ROC curve analysis showed that the area under the curve of PT, NLR and PCT/ALB combined predicted the death of sepsis patients with BSI was 0.897, which was larger than that predicted by PT, NLR and PCT/ALB alone (P<0.05). Conclusion: Compare with fungi and G+ bacteria, PT, NLR and PCT/ALB of G-bacteria in sepsis patients with BSI are significantly elevated, and the PT, NLR and PCT/ALB elevated are independent risk factors affecting the prognosis of sepsis patients with BSI, and the combination of the three has higher predictive value for the prognosis of sepsis patients with BSI. [ABSTRACT FROM AUTHOR]

Type 2 diabetes mellitus (T2DM) is a common chronic metabolic disease, while there is no consistent conclusion on the pathogenesis of T2DM. With the innovation of microecological research technology, intestinal dysbiosis is considered to be one of the causes of T2DM. Exercise is one of the effective means of diabetes management, while mechanism of regulating glucose metabolism still has many problems to be solved. In recent years, people have found that exercise can improve the intestinal environment and intestinal flora. This article will review the research on the influence and mechanism of exercise on T2DM based on intestinal flora, aiming to provide new ideas for exploring how exercise can improve T2DM in the future. [ABSTRACT FROM AUTHOR]

Brain and muscle arnt-like 1 (Bmal1) as one of the core genes of the clock geneis mainly expressed in the female reproductive system. The circadian rhythm of Bmal1 expression is involved in estrous cycle, ovulation, implantation, and the maintenance of pregnancy. The changed expression of Bmal1 is related to spontaneous abortion, possibly by a variety of mechanisms. Down-regulation of Bmal1 expression can decrease the expressions of matrix metalloproteinase 2/9 through SP1 -DNMT1/DAB2IP pathway, which followed by the inhibition of trophoblast migration and invasion. The deletion of Bmal1 gene interferes with the transcription and translation of steroid synthesis-related genes in ovarian granulosa cells, which affects the secretion of pregnancyrelated hormones and promotes the apoptosis of granulosa cells through PI3K/Akt/mTOR signaling pathway. The decreased expression of Bmal1 gene can cause the increased reactive oxygen species in ovary and fallopian tube, which can reduce oocyte fertilization, early embryo development and implantation potential, and eventually lead to embryo loss. In this article, the influence of Bmal1 on early spontaneous abortion was reviewed. [ABSTRACT FROM AUTHOR]

Objective To explore the continuous quality improvement to enhance the application effect of dental clinic nursing staff hand hygiene．Methods From June 2015 to December 2015，a comprehensive and continuous hand hygiene intervention was carried out among the nurses in dental clinic and the knowledge of hand hygiene，the rate of hand washing and the correct operation of hand washing were carried out．Results The hand washing rate of the nurses increased from 55% to 100%，the mastery of hand hygiene knowledge and the qualified rate of hand washing operation reached 100%．The results of helicobacter pylori colony culture showed that the number of average colonies decreased obviously．Conclusion Infection control should start from hand washing and comprehensive and continuous quality improvement should be carried out so as to improve the compliance of hand hygiene of nursing staff and strengthen the consciousness of hand washing, which is of great significance for the infection control． [ABSTRACT FROM AUTHOR]

In this paper,whey protein isolate(WPl) ,and casein(CS) were employed as emulsifiers to prepare reasonably stable oil-in-water emulsions and the effects of protein concentation, continuous phase pH,the concentration of NaCl on oxidative stability of emulsion were also evaluated. The results showed that increasing protein concentration led to decreased lipid hydroperoxide (ROOH) and thiobarbituric acid-reactive substances (TEARS). pH had a great influence on lipid oxidation. At pH 3. O,lipid oxidation rates were lower than those of pH 7. 0,8. 0 and 9. 0. Lower NaCl concentrations improved the antioxidant activity of proteins,and reduced the lipid oxidation. However, higher NaCl concentrations had the opposite effects. [ABSTRACT FROM AUTHOR]

Objective: To analyze the therapeutic effect of Tinidazole mixture(mainly tinidazole, supplemented by dexamethasoiie and cephalosporins thiamethoxam) on the dental pulp disease. Methods: A total of 90 cases of dental pulp disease patients admitted in our hospital from 2013-2015 were randomly divided into the experimental group and control group. The experimental group was given tinidazole mixture combined with formaldehyde cresol for temporary stopping and for permanent filling treatment after 1 week. The control group was given formaldehyde cresol for temporary stopping and for permanent filling treatment after 1 week. The treatment of both groups lasted for 1 week. The therapeutic effect, clinical symptoms improved conditions of the two groups were compared, and the two groups of patients were followed up for 6 months after the treatment to observe the recurrence rate and incidence of complications. Results: The total effective rate of experimental group(97.8 %) was significantly higher than that of the control group(82.4 %) (P<0.05). The recurrence rate of experimental group in 6 months was 2.2 %, winch was significantly lower than that of the control group (13.3 %) (P<0.05). And the incidence of adverse reactions in experimental group (13.3 %) was lower than that of the control group (77.8 %)(P<0. 05). Conclusions: Tinidazole mixure had great therapeautic effect in the treatment of dental pulp disease with low recurrence rate and fewer adverse reactions. [ABSTRACT FROM AUTHOR]

Objective: To analyze the effect of sevoflurane and remifentanil combined anesthesia on postoperative cognitive function in patients undergoing gynecological laparoscopic myomectomy. Methods: 148 patients undergoing laparoscopic removal of uterine fibroids were selected and randomly divided into observation group and control group. The observation group adopted sevoflurane remifentanil anesthesia, and the control group took propofol remifentanil intravenous anesthesia. The effect on postoperative cognitive function of the two kinds of anesthesia was compared. Results: Among patients in control group, the cognitive function score 1 day after the operation was lower than that 1 day before operation, and the difference was statistically significant (P<0.05). Among patients in observation group, the cognitive function score 1 day after the operation was higher than that 1 day before operation, and the difference was statistically significant (P<0.05). In control group, the TMT completion time 1 day after operation is more than that 1 day before operation, and the difference was statistically significant (P<0.05). In observation group, the TMT completion time 1 day after operation is less than that 1 day before operation, and the difference was statistically significant (P<0.05). The occurrence rate of postoperative respiratory inhibition, nausea and vomiting, restlessness, dizziness, lethargy in observation group was lower than that in control group, the difference was statistically significant (P<0.05). Conclusion: Sevoflurane and remifentanil combined anesthesia has less influence on postoperative cognitive function in patients under laparoscopic myomectomy, and is relatively safe, which is a more suitable anesthesia method. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of miRNA-219-5p on the proliferation， apoptosis， and invasion of pancreatic carcinoma cells and its mechanism. Methods Real-time fluorescence quantitative PCR (qPCR) was carried out to determine miR-219-5p expression in the pancreatic carcinoma cell lines Panc-1，AsPC-1，and Bxpc-3 and normal pancreatic ductal epithelial cell line HPDE6-C7. Further, Panc-1 cells were chosen and were divided into two groups： the normal control group (NC group) (transfection with miR-219-5p scramble) and miR-219-5p group (transfection with miR- 219-5p mimics). The cell proliferation and apoptosis were detected by MTT assay and flow cytometry. The invasion ability was checked by transwell assay. The expression of epidermal growth factor receptor (EGFR) and Cleaved Caspase-3 proteins was examined by Western blotting. Results MiR-219-5p expression was lower in pancreatic carcinoma cell lines Panc-1，AsPC-1，and Bxpc-3，as compared with that in the pancreatic ductal epithelial cell line HPDE6-C7 (all P < 0. 01). The 0D490nm of the miR-219-5p group was significantly lower than that of the NC group at 72，96，and 120 h ( all P <0.01)； the apoptosis rate was higher (all P <0. 01). The invasive cells were (75. 3 ± 6. 9)/Hp in the miR-219-5p group，which was significantly lower than that [(172.5 ±10.5)/Hp] in the NC group (P <0.01 ). The expressive level ofEGFRinthemiR-219-5pgroupwassignificantlylowerthanNCgroup(0.31±0.09vs.1.0，P<0.01). The expression of Cleaved Caspase-3 was up-regulated in the miR-219-5p group as compared with that of NC group (2.7 ±0. 15 vs. 1.0, P <0.01). Conclusions The miR-219-5p is lowly expressed in pancreatic carcinoma cell lines. The over-expression of miR-219-5p can inhibit the proliferation and invasion of pancreatic carcinoma cells, and induce the apoptosis, possibly though the down-regulated protein expression of EGFR and up-regulated protein expression of Cleaved Caspase-3. [ABSTRACT FROM AUTHOR]

Objective: To compare the differences in clinical application value of ApexitPlus calcium hydroxide paste and zinc oxide iodoform paste combined with gutta percha point in the one-visit root canal therapy for treating patients with chronic pulpitis. Methods: 110 patients (124 teeth) with chronic pulpitis were selected as the research objects. On the premise of patients' agreement, the patients were divided into the ApexitPlus calcium hydroxide paste group (group A, n=55, 63 teeth) and the zinc oxide iodoform paste group (group B, n = 55, 61 teeth) of two groups according to the random number table method. The two groups of patients were given one-visit root canal therapy, with a period of 12 to 24 months of follow-up in behavior phase. The effect of root canal filling after operation and the clinical curative effect were compared. The differences in pain scores and sleep quality scores before and after the treatment were recorded and the incidence of postoperative emergency response was analyzed. Results: ①There were no statistical significances in the effect of root canal filling between the two groups (P>0.05). The treatment success rate of group A in 3 months after operation (100.0%) was significantly higher than that of group B (91.8%) (P<0.05). The treatment success rate of group B at the 24th month of follow-up (83.6 %) was significantly lower than that of group A (96.8 %) (P<0.05); ②After the treatment, the visual analgesia score (VAS), Pittsburg sleep quality index (PSQI) and other treatment indexes of the two groups were significantly lower than those before the treatment and the decline in group A was greater than that in groupB (P<0.05); ③In 7d after operation, the occurrence rate of postoperative emergency response in groupA (10.9 % ) was significantly lower than that in groupB (38.2%) (P<0.05). Conclusion: To treat patients who have chronic pulpitis with ApexitPlus calcium hydroxide paste and gutta percha point as the filling materials for one-visit root canal therapy, the curative effect is exact and the root filling effect is good, which can effectively reduce the risk of postoperative emergency response and have a positive impact on improving the therapeutic effect, the prognosis and quality of life of patients. It is worth clinical promotion. [ABSTRACT FROM AUTHOR]

目的探讨延续性护理干预对极低出生体重儿智力发育、体格发育的影响。方法选取2016 年7 月 22 日至2017 年7 月22 日该重症监护室收治的极低出生体重儿120 例作为研究对象，采用计算机分组模式将其分为 观察组和对照组，各60 例。观察组进行延续性护理干预措施，对照组进行常规护理。结果观察组小儿的护理后3 个月 智力恢复评分、护理后6 个月智力恢复评分、对安慰的反应、前臂弹回速度、手握持能力、再次入院率、神经发育受损率、 胎便排尽时间和体重恢复时间均优于对照组，差异均有统计学意义（P<0.05）。结论对极低出生体重儿实施延续性护 理干预措施效果显著。 [ABSTRACT FROM AUTHOR]

目的　观察长链非编码 ＲＮＡ（ｌｎｃＲＮＡ）ＢＡＮＣＲ 在胃癌细胞中的表达变化，及其对细胞增殖、迁移能力的影响，并探讨其机制。方法　采用 ＲＴ-ＰＣＲ 法检测胃癌细胞 ＡＧＳ、ＢＳＧ８２３、ＭＧＣ-８０３、Ｈｓ７４６Ｔ 和正常胃上皮细胞 ＧＥＳ-１ 中的ｌｎｃＲＮＡ ＢＡＮＣＲ。将 ＡＧＳ 细胞分成ＬＶ-ＢＡＮＣＲ ｓｈＲＮＡ 组、ＬＶ-ＮＣ 组及 ＢＬＡＮＫ 组，采用 Ｌｉｐｏ- ｆｅｃｔａｍｉｎｅ２０００ 分别转染 ｐｃＤＮＡ３． １-ＬＶ-ＢＡＮＣＲ ｓｈＲＮＡ、ｐｃＤＮＡ３． １-ＬＶ-ＮＣ 及 ＰＢＳ；分别于培养 ０、１、２、３、４ ｄ 后采用 ＭＴＴ 试验检测细胞增殖能力，采用细胞划痕试验检测细胞迁移能力，采用 Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ 法检测细胞中的核转录因子 ＮＦ-κＢ１ ｐ５０、ＮＦ-κＢ１ ｐ１０５ 及磷酸化细胞外信号调节激酶（ｐ-ＥＲＫ）。结果　ＡＧＳ、ＢＳＧ８２３、ＭＧＣ-８０３、Ｈｓ７４６Ｔ 细胞中ｌｎｃＲＮＡ ＢＡＮＣＲ 相对表达量高于 ＧＥＳ-１ 细胞（Ｐ 均＜ ０． ０５）。培养３、４ ｄ 后，ＬＶ-ＢＡＮＣＲ ｓｈＲＮＡ 组ＯＤ 值小于 ＬＶ-ＮＣ 组和 ＢＬＡＮＫ 组（Ｐ 均＜０． ０５）。ＬＶ-ＢＡＮＣＲ ｓｈＲＮＡ 组划痕愈合率低于ＬＶ-ＮＣ 组（Ｐ ＜ ０． ０５）。ＬＶ-ＢＡＮＣＲ ｓｈＲＮＡ 组 ＮＦ-κＢ１ ｐ５０、ＮＦ-κＢ１ ｐ１０５、ｐ-ＥＲＫ 相对表达量均低于ＬＶ-ＮＣ 组（Ｐ 均＜ ０． ０５）。结论　ｌｎｃＲＮＡ ＢＡＮＣＲ 在胃癌细胞中高表达。沉默 ｌｎｃＲＮＡ ＢＡＮＣＲ 表达可抑制胃癌细胞的增殖、迁移能力，其机制可能与ＮＦ-κＢ１ ｐ５０、 ＮＦ-κＢ１ ｐ１０５ 及 ｐ-ＥＲＫ 表达下调有关 . [ABSTRACT FROM AUTHOR]

目的探讨高频电针治疗腋臭术后复发的效果,同时对腋臭复发因素及复发后治疗方法进行文献回顾。方法自 2015 年3 月至2018年10月,选择腋臭术后复发患者22 例，采用高频电针进行治疗。术后6 个月，对治疗效果按照腋臭分级标 准进行评判，观察高频电针治疗腋臭术后复发的治愈率。结果本组22 例获随访6~9 个月，12 例为0 级，9 例为1 级，1 例为2 级，治愈率为95.45%（21/22）。结论高频电针治疗腋臭术后复发疗效确切，损伤较小，容易被患者接受，是一种值得推荐的治 疗方法。 [ABSTRACT FROM AUTHOR]

Objective To observe the effects of linseed oil on body weight, blood sugar and gastrointestinal hormone levels in obese and type 2 diabetic mice induced by high-fat diet. Methods Thirty male C57B1/6J mice were randomly divided into the control group, model group, and experimental group, with 10 mice in each. The mice in the model group and linseed oil group were fed with high-fat diet for 12 weeks to establish the obese type 2 diabetic models. From the 13th week, the mice in the model group were fed with high-fat diet, the experimental group with high-fat diet containing linseed oil (15 g/100 g diet), and the control group with normal diet. After 24 weeks of treatment, body composition, blood glucose, the levels of insulin, glucose-dependent insulinotropic polypeptide (GIP) and Leptin in the plasma were measured Results The body mass and body fat weight of the experimental group and the model group were higher than those of the control group, and the weight of lean tissues was lower than that of the control group (all P< 0. 05). Compared with the model group, the experimental group mice had increased lean body mass but decreased body fat, the difference was statistically significant (both P< 0. 05). Fasting blood glucose, insulin and leptin levels in the model group and the experimental group were all higher than those in the control group, and fasting GIP level in the model group was higher than that in the control group (P< 0. 05). The levels of blood glucose, insulin, GIP and leptin in the experimental group were significantly ower than those in the model group (all P< 0. 05). Conclusion Linseed oil can reduce the body weight, blood sugar and insulin in obese and type 2 diabetic mice, which may be related to the regulation of GIP and leptin levels. [ABSTRACT FROM AUTHOR]

BACKGROUND: Olfactory dysfunction is an early biological marker of various diseases. However, the neuroimaging mechanism by which olfactory dysfunction occurs following cerebral small vessel disease is unclear. OBJECTIVE: To explore the different neuroimaging mechanisms of olfactory function regulation in patients with cerebral small vessel disease and Parkinson’s disease, and explore the potential application value of olfactory function assessment in patients with cerebral small vessel disease. METHODS: Neuropsychological and olfactory tests, high-resolution structural magnetic resonance and resting-state functional magnetic resonance data were collected in 80 patients with cerebral small vessel disease, 44 healthy controls and 29 patients with Parkinson’s disease. DPABI, SPM12 and SPSS were used to analyze and compare the amplitude of low frequency fluctuation, regional homogeneity and functional connectivity values between the cerebral small vessel disease, control and Parkinson’s disease groups. Correlations between the significantly altered resting-state functional magnetic resonance imaging measures and olfactory and cognitive scores were evaluated. RESULTS AND CONCLUSION: Compared with the control group, low-frequency fluctuation amplitude of the right dorsolateral superior frontal gyrus and the regional homogeneity of the left wedge leaf were significantly reduced in the cerebral small vessel disease and Parkinson’s disease groups. The right dorsolateral superior frontal gyrus and the left cuneiform lobe are the seed points. Compared with the Parkinson’s disease group, the functional connectivity values of the right anterior cunei, inferior temporal gyrus, anterior central gyrus and dorsolateral superior frontal gyrus, left posterior central gyrus and inferior temporal gyrus were significantly enhanced in the control and cerebral small vessel disease groups. The left cuneiform lobe was the seed point. Compared with the control group, the functional connectivity of the left lingual gyrus was significantly weakened in the cerebral small vessel disease and Parkinson’s disease groups. The functional connectivity values of the left middle temporal gyrus and the right posterior central gyrus were enhanced in the control group compared with the cerebral small vessel disease and Parkinson’s disease group, and that was enhanced in the cerebral small vessel disease group compared with the Parkinson’s disease group. Correlation analysis showed that the olfactory score and cognitive score were positively correlated in the cerebral small vessel disease group, and the regional homogeneity of the left wedge lobe was negatively correlated with the Montreal Cognitive Assessment Scale score, while the functional connectivity of left wedge lobe-left middle temporal gyrus in the Parkinson’s disease group was positively correlated with the olfactory recognition score, and the functional connectivity values of the left wedge lobe-left posterior central gyrus and left wedge lobe-left lingual gyrus were positively correlated with the olfactory identification score and the total olfactory score, respectively. The regulation of olfactory function in patients with cerebral small vessel disease has a different neuroimaging mechanism from that of olfactory dysfunction in patients with Parkinson’s disease. The olfactory function of patients with cerebral small vessel disease is related to cognitive function. It is speculated that the olfactory function following cerebral small vessel disease is a secondary change of brain dysfunction, while olfactory dysfunction following Parkinson’s disease is directly caused by abnormal function of olfactory-related brain areas. Olfactory function assessment in patients with cerebral small vessel disease has potential application in predicting cognitive function. [ABSTRACT FROM AUTHOR]

Pregnant women's thyroid is in a special physiological period during pregnancy. Thyroid level plays a key role in maintaining normal pregnancy and fetal growth and development. Subclinical hypothyroidism (SCH) is the most common thyroid dysfunction during pregnancy. Most studies have shown that SCH can lead to an increased risk of adverse pregnancy outcomes, however, its clinical diagnosis and treatment are still under revision. This article reviewed the current status and research of SCH during pregnancy. [ABSTRACT FROM AUTHOR]

Although deep learning-based image coloring methods have achieved significant results, but there are still suffer from three problems: redundant stain, color dimming, and color deviation. To this end, this paper proposed an instance image coloring method combined with fine-grained attention (fine-grain self-attention, FGSA). Specifically, it firstly divided the extracted feature maps into color and spatial location, and combined the two parts of the fittingto improve the correspondence between the color and the spatial location of the image to mitigate the redundant color patches. Secondly, inspired by the principle of HDR for optical photography, it utilized convolutional kernels with small sensory fields to enhance or suppress the color features of the image, and combined them with softmax to dynamically map the features, thus improving contrast and alleviating the darkness of the coloring. Finally, combining different nonlinear basis functions increased the network’s representation of nonlinear colors and fitted a color distribution that was closest to the real image to address color bias. Extensive experimental results show that the proposed method achieves satisfactory results in instance image coloring. In particular, compared with the state-of-the-art methods, the proposed method improves 4.1% and 7.9% in feature perception evaluation indexes LPIPS and FID, respectively. [ABSTRACT FROM AUTHOR]

目的探讨利用体外冲击波碎石术（ESWL）联合经内镜逆行胰胆管造影术（ERCP）治疗慢性胰腺炎合 并胰管结石的效果。方法选取2014 年1 月至2017 年1 月该院消化内科收治的慢性胰腺炎合并胰管结石患者40 例。所有患者根据情况行ESWL 或联合ERCP 手术治疗，观察患者治疗情况及并发症发生情况；术后随访半年，采用视 觉疼痛评分（VAS）评价患者疼痛改善情况，采用生活质量评定表（SF⁃36）评价患者生活质量。结果40 例患者均顺利 完成手术，共进行了64 次ESWL，其中经历1 次ESWL 4 例，经历2 次ESWL 18 例，经历3 次ESWL 6 例，经历5 次ES⁃ WL 12 例，完全碎石率为70.0%（28/40），并发症发生率为10.0%（4/10）。12 例患者经ERCP 取石，均取石彻底，无残留。40 例患者术后VAS 为（82.4±11.3）分，SF⁃36 评分为（2.5±1.0）分，均显著优于术前的（74.6±8.2）分及（5.1±1.4）分，差异均 有统计学意义（P<0.05）。结论ESWL 联合ERCP 治疗慢性胰腺炎合并胰管结石的效果较好，可提高结石清除率，且安 全性较高，有助于改善预后。 [ABSTRACT FROM AUTHOR]

目的探讨品管圈管理模式下的电话随访对抑郁症出院患者服药依从性的影响。方法选取该科2014年8月至2016年2月出院的142例抑郁症患者，按具体采用的随访模式分为对照组（63例）和观察组（79例）。对照组按常规方法进行电话随访，观察组在此基础上给予品管圈活动干预，比较2种随访模式对于改善抑郁症出院患者服药依从性的效果。结果观察组服药依从良好率[79.8%(63/79)]明显高于对照组[61.9%(39/63)]，而观察组服药依从不良率[6.3%(5/79)]明显低于对照组[17.5%(11/63)]，差异均有统计学意义（P<0.05);两组间服药依从性中等率比较，差异无统计学意义（P>0.05)。结论品管圈管理模式下的电话随访较常规电话随访更能有效维持抑郁症出院患者的服药依从性。 [ABSTRACT FROM AUTHOR]

In an era of increasing global retail sales and same-day delivery, fast delivery and quality batching of orders are the key factors affecting the picking efficiency of RMFS system. In order to construct high-quality order allocation batches and improve the order-picking efficiency of RMFS, an improved differential evolution algorithm (LNS＿DE) is proposed, which integrates the idea of destruction and repair of large neighborhood search, a batch of removal operators based on random, maximum cost contribution and centralized batches, and a new insertion operator component. The order batching optimization model is established with the objective of minimizing the total delay time of orders, and the simulation experiments are carried out for different order sizes. The simulation results show that the proposed order batching optimization algorithm has better solution quality, more stable performance and faster convergence speed than the differential evolution algorithm. Especially when the number of orders increases, the average optimization proportion of the LNS＿DE algorithm solution expands, which provides effective decision guidance for improving the order-picking efficiency of RMFS system and achieving rapid response to orders. [ABSTRACT FROM AUTHOR]

Actinic keratosis (AK) is a skin lesion caused by atypical keratinocyte proliferation， which is considered to be a precancerous lesion of squamous-cell carcinoma and basal cell carcinoma. It is now maintained the long-term ultraviolet radiation causes DNA damage of keratinocyte， which can not be repaired. Then mutation occurs, which eventually results in activation of oncogene and inhibition of tumor suppressor gene, leading to the occurrence of AK. Currently， the large sample investigation and research on the risk factors of AK are relatively limited, and the related data are rare. In this paper, the research progress of the risk factors for AK in recent years is reviewed. [ABSTRACT FROM AUTHOR]

Ovarian structure and functions are important for female fertility. The development and maturation of reproduction, and maintains of ovarian function and fertility, are mainly regulated by the hypothalamic -pituitary -ovarian (HPO) axis and the local factors of ovary, such as transforming growth factor-P (TGF-P)，epidermal growth factor (EGF) and insulin-like growth factor (IGF). It was reported that the adverse maternal environment during pregnancy (such as prenatal xenobiotics exposure, maternal nutrition disorder and disease, etc.) was associated with the reproductive development in offspring, which would lead to diminished ovarian reserve such as premature ovarian failure (POF) in some adult offspring. The mechanism of fetal-derived POF is related to the theory of "intrauterine programming", including they abnormalities of neuroendocrine programming and epigenetic modification. In this review, we discussed the intrauterine programming mechanism of fetal-derived POF, and the potential markers of early warning, prevention and treatment of this disease. [ABSTRACT FROM AUTHOR]

糖尿病性乳腺病是一种罕见的乳腺良性疾病, 临床与影像学表现均与乳腺癌类似, 容易 误诊。该文对天津医科大学总医院收治的2例糖尿病性乳腺病的患者进行报道。例1为绝经前47岁 1型糖尿病患者, 其应用胰岛素30年, 长期血糖控制不佳, 此次主因"发现双侧乳腺肿块6年"就诊, 乳 腺超声及钼靶均提示双侧乳腺占位, 最终行穿刺活检, 确诊为糖尿病性乳腺病。例2为绝经后60岁 2型糖尿病患者, 其应用胰岛素2年, 长期血糖控制不佳, 此次主因"发现左乳腺肿块2个月"就诊, 乳 腺超声及钼靶提示左乳占位, 最终行穿刺活检, 确诊为糖尿病性乳腺病。2例患者均未行手术治疗, 随诊中。该文对该2例糖尿病性乳腺病患者的临床诊疗经过进行报道, 以期提高临床医师对该疾病 的认识, 避免过度手术。. [ABSTRACT FROM AUTHOR]

Basal cell carcinoma （BCC） , a common non-melanoma skin cancer， is occasionally with deep invasion， destruction of adjacent structures， recurrence ， but it rarely occurs distant metastasis. Some studies show that abnormal Hedgehog pathway may be associated with the occurrence of BCC.The establishment of animal models confirmed the close relationship between this pathway and BCC， which laid the foundation for the study of disease therapy. Small molecule inhibitors such as Vismodegib block this signaling pathway and inhibit tumor growth. Recent genomic studies point to the involvement of additional genetic mutations that might be associated with the development of BCC， suggesting significance of other signaling pathways， such as WNT， mTOR， aside from hedgehog in thepathogenesis of this human tumor，and this paper reviews the relationship between Hedgehog pathway and BCC. [ABSTRACT FROM AUTHOR]

Objective To construct an animal model of squamous cell carcinoma of the skin in SKH-1 mice with ultraviolet irradiation， and to investigate the DNA-PKcs activation level of DNA-PKcs-mTORC2/Akt signal transduction pathway during UV-induced skin SCC and AK to SCC transformation. Methods 43 SKH-l hairless mice were randomly divided into experimental group （33） and control group （10） .The experimental group used the SUV-1000 solar simulator to simulate sun-irradiated SKH-1 hairless mice， and the control group did not intervene. Experimental group 1 maintained irradiation for 24 weeks， and experimental group 2 maintained irradiation for 16 weeks. The mice were sacrificed at 12， 18， 24， and 28 weeks for skin histopathological examination. At 28 weeks， the same skin of the same mouse was taken for histopathological examination and immunohistochemistry. Five cases of adult solar keratosis （AK），cutaneous squamous cell carcinoma （SCC）， normal light-protected site （NNS）， and normal exposed site （NES） were collected for WesternBlot. Results After 12 weeks， some mice in the experimental group were thickened in the back skin. At 17 weeks， the papules with diameter ≥ 1 mm appeared in the back skin of experimental group . After 28 weeks of irradiation， the tumor formation rate reached 100%. No tumor formation was observed in the control group. Both DNA-PKcs and p-DNA-PKcs （T2609） were localized in the cytoplasm and nucleus. Expression of DNA-PKcs increased in epidermal tissue. There was a difference in the statistical analysis of DNA-PKcs expression （P＜0.05）， but there was no difference in the expression of p-DNA-PKcs （T2609） （P＞0.05） .The expression levels of DNA-PKcs and p-DNA-PKcs （T2609） in human epidermal tissue increased and there were statistical differences （P＜0.05） .Conclusions We successfulyl prepared of mouse AK model and SCC model. It is clear that UV-induced squamous cell carcinoma of the skin can induce DNA damage and repair. [ABSTRACT FROM AUTHOR]

To explore the prognostic value of cuproptosis-related genes in cervical cancer, the clinical data of cervical cancer patients were downloaded from the TCGA database and randomly divided into training group and validation group. Cuproptosis-related genes were screened out by univariate Cox, LASSO-Cox and multivariate Cox analysis, and the risk model was constructed. Overall survival (OS) of the two subgroups and the entire cohort was analyzed by Kaplan-Meier curve, and the prognostic value of the model was verified by ROC curve and PCA. Univariate and multivariate analyses were performed to evaluate the independent prognostic value of clinical features and risk scores. The biological functions and pathways between the two subgroups were analyzed by gene ontology (GO) and Kyoto encyclopedia enrichment of genes and genomes (KGEE), and the sensitivities of the two subgroups to drugs were further analyzed. Finally, prognostic models of five cuproptosis-related genes (FDX1, ARF1, APP, HSF1, MT1A) were constructed. From the survival curve of risk score, OS in the low-risk group was much higher than that in the high-risk group, and the prognosis was good (P<0.05). By univariate and multivariate Cox analysis, risk score an independent prognostic factor (P<0.001). The predictive power of the prognostic model was demonstrated by receiver operating characteristic curve (ROC) and principal component analysis (PCA). ROC curve analysis was used to assess the sensitivity and specificity of risk scores and other clinical features, such as age, grade, and stage. And the results showed that the prognostic value of risk scores was superior to other clinical features. The results of enrichment analysis showed that gene function was mainly concentrated in extracellular matrix and extracellular structure. According to TIDE algorithm, immunotherapy efficacy of patients in the low-risk group was superior to that in the high-risk group. In addition, significant differences were found in the sensitivity of 24 drugs between the two subgroups. The study established a prognostic risk model composed of 5 cuproptosis-related genes, and proved that the model can accurately predict the prognosis of patients. And patients with low risk score are more likely to benefit from immunotherapy, which provides theoretical basis for clinical individualized therapy. [ABSTRACT FROM AUTHOR]

In order to provide theoretical information for the effective utilization value of the moss Haplocladium microphyllum (Hedw.) Broth., we measured the general components included total sugar, crude fat, crude protein and ash. The compositions and contents of amino acids in moss were determined by automatic amino acid analyzer. For making known the content and composition of fatty acids in moss oil and then offering reference to the reasonable exploitation, we made the analysis on fatty acid content and construction variation of fatty acid with high performance gas chromatography. We determined mineral element contents by inductively coupled plasma-atomic emission spectrometry, and analyzed the stem and leaf elemental distribution and average mass fraction by SEM X-ray microanalysis method. The results showed that total sugar in H. microphyllum reached up to 16.11%, the contents of crude protein and ash were 11.20% and 23.34%, respectively. The amino acid composition of protein was in balance with EAA/TAA 27.70% and the ratio of EAA to NEAA 0.38. In H. microphyllum, there was the limiting amino acid histidine with SRC 12.96. The content of medicinal amino acid was 62.12% of total amino acid, and the contents of aspartic acid, glutamic acid, arginine and leucine were relatively higher in eighteen kinds of amino acids. The crude fat content in H. microphyllum was 0.32% with the ratio of polyunsaturated fatty acids to total fatty acids as 38.21%. The ratio of unsaturated fatty acids to saturated fatty acids was 0.74. The content of palmitic acid was the highest, followed by acidum linoleicum and alpha-linolenic acid, while arachidonic acid and docoshexaenoic acid were relatively high in unsaturated fatty acids. In addition, the mineral element contents of the moss were very rich and various. As the beneficial trace elements, the contents of K, Ca, Mg, P and Fe were relatively high. Between stem and leaf, the elemental composition was the same, but average mass fraction was slightly different. The distribution of chemical elements in stem was uniform, similar to that of the leaf. Therefore, H. microphyllum was a biological resource to be developed, which contained lower fats and proteins, richer minerals and higher sugars. [ABSTRACT FROM AUTHOR]

Targeted therapeutic drugs for acute myeloid leukemia(AML)are showing immense development, thereby laying a solid foundation for the precise treatment of AML patients. The paper reviews four types of targeted drugs that have progressed rapidly for AML treatment(by targeting genes or signaling-pathway alterations, targeting apoptosis-related pathways, targeting cell-surface antigens, and targeting immune-related substances). We look forward to the future development directions of targeted drugs, providing references for hematologists and developers of new drugs for AML. [ABSTRACT FROM AUTHOR]

Objective · To prepare chitosan-gelatin porous microspheres by high voltage electrostatic method combined with freeze-drying and ionic cross-linking method and investigate the factors that influence the formation of porous medium. Methods · Porous chitosan microspheres and chitosangelatin porous microspheres were prepared using high voltage electrostatic method combined with freeze-drying and ionic cross-linking method, with sodium tripolyphosphate (STPP) as crosslinking agent. Factors that affect the porous structure and pore size of porous microspheres were compared, such as different chitosan-gelatin ratio, freezing temperature, curing time with saturated STPP in 85% ethanol solution. The morphology, surface and internal structure, particle size of the porous chitosan microspheres and chitosan-gelatin porous microspheres were observe by using light microscope, scanning electron microscope and hematoxylin-eosin staining. Results · Microspheres prepared by freeze-drying with an electrostatic and ionic cross-linking method have open, interconnected and highly macroporous, with good spherical surface. Saturated STPP ethanol solution (85% ethanol) was chosen as the crosslinking agent to prevent destruction of the porous structure. The order of freeze and crosslinking, cross-linking time and the second freezing temperature, can influence the pore size of porous microspheres. Gelatin and chitosan can form polyelectrolyte complexes, and can also be used as porogen in porous structure. Conclusion · The preparation of porous chitosan-gelatin microspheres via this method has a large pore size (diameter 100-200 µm), suitable for cell growth and the migration. [ABSTRACT FROM AUTHOR]

The uniaxial stress-strain relationships in parallel-to-grain direction of GLB were firstly studied by materials quality testing. Then 15 laminated bamboo beam specimens were designed and tested to investigate the flexural behaviors and the damage mechanism. It is concluded that the tensile stress-strain relationship in parallel-to-grain direction of GLB exhibits linear behavior, and that the compressive one has obvious linear and nonlinear stages, which can be simulated by tri-linear relationship. The tensile strength in parallel-to-grain direction of GLB is higher than its compressive strength by 58. 7% , and its tensile modulus is higher than the compressive modulus by 25.4%. The results of the bending test indicate that the plane-section assumption is valid. The neutral axis of the bending section shifts towards its compression side with the increase of load. Finally, an analytical model to estimate the bending performance of GLB beams was proposed based on experimental studies and theoretical analysis. Well agreements are achieved between the results obtained by using the proposed model and those obtained by experiments. [ABSTRACT FROM AUTHOR]

Intramural pregnancy is a special type of ectopic pregnancy that is extremely rare in clinic. If the pregnancy continues to second or third trimester of pregnancy, it is very likely to lead to uterine rupture, severe hemorrhage, shock, loss of fertility, and even fatal in severe cases. A case of an intramural pregnancy patient with uterine rupture at 19 weeks was reported. Combined with related case reports, firstly the initial abdominal pain of pregnancy was considered, and then it might be uterine rupture, finally the surgical and postoperative review analysis verified that it was intramural pregnancy rupture. By reviewing the diagnosis and treatment of this patient, summarizing and reviewing relevant literatures, we will strengthen the understanding of such rare pregnancy in order to detect, identify and treat this rare pregnancy earlier, so as to improve the adverse pregnancy outcomes of patients. [ABSTRACT FROM AUTHOR]

Under the situation of multi-source water supply pattern of Shandong Province, in order to effectively solve the absorption issue of high-priced external water in the receiving areas of South-to-North water Transfer Project, and promote the efficient utilization of water resources, this paper built a model of multi-source comprehensive water price for industry based on refined and optimized allocation. Different water sources were distributed to users at the same comprehensive price. At the same time, the influence of water quantity variation of different water sources to the comprehensive water price was studied, and the formation rule of comprehensive water price was explored. Taking the actual situation of water resources development and utilization in Zouping City as an example, it analyzed the comprehensive water price of the multi-source water supply system of industrial water. The results show that the comprehensive water price in 2020 and 2025 arc 3.88 Yuan/m3 and 3.89 Yuan/m³ respectively. Finally, it selected the typical water sources as the unce11ainty factors to analyze their influences to the comprehensive water price. [ABSTRACT FROM AUTHOR]

Objective To establish a druggability evaluation method for new targets of anti-tumor drugs by analyzing the mutation genes of common tumors in the digestive system. Methods We collected the mutant gene data of the five common tumors of the digestive system (esophageal cancer, gastric cancer, colorectal cancer, liver cancer and pancreatic cancer) in the Integrative Onco Genomics database, and screened out the genes with higher mutation rates in each tumor. We evaluated the druggability of these genes or their encoded proteins, and discovered the potential targets for the new anti-tumor drugs. Results A total of five tumors, 35 cohorts and 5445 tumor samples were collected in this study. The top 10 mutation genes were selected for further analysis. The canSAR database was used to analyze the druggability of unpublished mutant genes or their encoded proteins, and a total of 17 potential therapeutic drug targets were screened out. Conclusion A method for evaluating druggability of targets based on mutant genes or their encoded protein is established in this study. The application of this method can provide a reference for discovering new anti-tumor therapeutic target, saving the cost and time of target screening in new drug development. [ABSTRACT FROM AUTHOR]

Colonoscopy had been considerably developed in maneuvers and instruments since the late nineteen sixties.It made the visualization,biopsy and treatment of intestine possible without laparotomy.Though its application had brought dramatic benefits for the diagnoses and treatments of colorectal diseases and became the gold standard for primary investigation of colorectal cancer,its limitations including unavoidable pain,unpredictable complications,impossibility of guaranteed intubation of the cecum and errors in colonoscopic detection of neoplasia appeared to be more and more obvious and made itself restricted,which negatively affected the patients’ compliance with the examine and increased the difficulty during the procedure.What was worse,some of them may miss the treatment which should have been available before.The clinical applications of conventional colonoscopy was reviewed so as to provide guidelines for the reasonable application of conventional colonoscopy hereafter. [ABSTRACT FROM AUTHOR]

Colonoscopy had been considerably developed in maneuvers and instruments since the late nineteen sixties. It made the visualization, biopsy and treatment of intestine possible without laparotomy. Though its application had brought dramatic benefits for the diagnoses and treatments of colorectal diseases and became the gold standard for primary investigation of colorectal cancer, its limitations including unavoidable pain, unpredictable complications, impossibility of guaranteed intubation of the cecum and errors in colonoscopic detection of neoplasia appeared to be more and more obvious and made itself restricted, which negatively affected the patients' compliance with the examine and increased the difficulty during the procedure. What was worse, some of them may miss the treatment which should have been available before. The clinical applications of conventional colonoscopy was reviewed so as to provide guidelines for the reasonable application of conventional colonoscopy hereafter. [ABSTRACT FROM AUTHOR]

目的 分析有创机械通气相关急性肾损伤的影响因素及其预测效能。方法 有创机械通气患者216例， 根据有创机械通气后是否发生急性肾损伤分为急性肾损伤组和非急性肾损伤组。采用单因素分析初步筛查出机械 通气患者发生急性肾损伤的影响因素，将有统计学意义的因素作为自变量进行Logistic回归分析。通过受试者工作 特征曲线（ROC）分析急性肾损伤的影响因素对有创机械通气患者发生急性肾损伤的预测效能。结果 216例有创 机械通气患者在有创机械通气48 h后有32例患者发生急性肾损伤。女性发病率高于男性（P<0. 01). 与非急性肾 损伤患者相比，急性肾损伤患者呼吸频率升高，BMI升高，平均动脉压降低，中心静脉压, 气道平台压升高，白细胞, C-反应蛋白水平升高，APACHE-Ⅱ评分, SOFA 评分升高，乳酸水平升高，pH 值降低，氧合指数升高（P 均< 0. 05). Logistic回归分析显示，呼气末正压, 中心静脉压, 乳酸是有创机械通气患者发生急性肾损伤的主要影响因 素。APACHE-Ⅱ评分, SOFA评分, 中心静脉压, 乳酸, 肌酐预测有创机械通气相关急性肾损伤发生的最佳诊断界值 分别为 16. 5 分, 11. 5 分, 14. 5 cmH2O, 2. 05 mmol/L, 131 µmol/L，ROC 下面积分别为 0. 80, 0. 94, 0. 89, 0. 94, 0. 73， 敏感度分别为0. 88, 0. 59, 0. 94, 0. 72, 0. 91，特异度分别为0. 63, 0. 78, 0. 83, 0. 96, 0. 92。结论 有创机械通气患者 发生急性肾损伤的发生率为 14％。呼气末正压, 中心静脉压, 乳酸是住院患者有创机械通气相关急性肾损伤的独 立影响因素。APACHE-Ⅱ评分, SOFA评分, 中心静脉压, 乳酸, 肌酐对有创机械通气相关急性肾损伤发生的预测效 能高。 [ABSTRACT FROM AUTHOR]