Search

Copyright of Petroleum Geology & Oilfield Development in Daqing is the property of Editorial Department of Petroleum Geology & Oilfield Development in Daqing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[ Objective]The purpose of this study was to analyze the associations of the VIP ( Vasoactive intestinal peptide, VIP), VIPR-1 ( Vasoactive intestinal peptide receptorl, VIPR-1) and VIPR-2 gene with chicken reproduction traits and find important markers for these traits. [ Method] PCR sequencing was used to detect the variation in the coding region of VIP gene, part of the intron region, and part of the sequence regions of VIPR-1 and VIPR-2 genes, and to analyze the association with reproductive traits. [Result] In the Yaoshan chicken pop ulation, one intron mutation site was detected for VIP gene, one coding region mutation site, one insert/ deletion mutation site and 14 intron region SNP sites were detected for VIPR-1 gene, and one coding region mutation site and two intron mutation sites were detected for VIPR-2 gene. 6 mutation sites (39 109, 39 114, 39 227, 68 817, 69 061, 97 123) of the VIPR-1 gene and 3 mutation sites ( 3739, 3184, 49 246) of the VIPR-2 gene were significantly associated with the total egg number with 300 d of age and broodiness traits ( P < 0. 05 or P < 0. 01). [Conclusion] The above polymorphic loci can be used as assisted selection markers for the reproductive traits of Llbo Y aoshan chick [ABSTRACT FROM AUTHOR]

[ Objective] The purpose of this study was to investigate the effects of light duration on the morphology and structure of ovary and fallopian tube and the main reproductive hormones of Guizhouhuang chickens. [ Method] The serum levels of follicle stimulating hormone ( FSH), luteinizing hormone ( LH) and estradiol ( E2) in Guizhouhuang chickens were measured at 140, 200 and 480 days of age, respec tively, under the condition of Half-housed breeding ( house feeding + sports field) and with natural light as the control, and the develop ment of main reproductive organs and the histological characteristics of reproductive organs were observed. [Result] At 140 days of age, ovi duct weight under increased light treatment was significantly lower than that under natural light treatment, and the maximum follicle diameter under light treatment was significantly higher than that under natural light treatment. There were no significant differences in ovary weight, o viduct length, the number of large yellow follicles and the number of small yellow follicles between the two treatments. At 200 days of age, there was no significant difference in ovarian weight and maximum follicle diameter between the two treatments. All the other indexes were significantly higher under increased light than those under natural light. At 480 days of age, the tubal weight of increased light treatment was significantly lower than that of natural light treatment, but there were no significant differences among other indexes. Increasing light had no significant effect on the ovarian tissue structure. The contents of FSH and E2 under increased light treatment were significantly higher than those under natural light treatment at 140 and 200 days of age. FSH, LH and E2 at 480 days of age had no significant differences among treatments. [Conclusion] Appropriate increase of light can improve the production performance of Guizhouhuang chicken. [ABSTRACT FROM AUTHOR]

[Objective] This study aimed to explore the correlations between TGFβ3 gene and reproduction traits in Liboyaoshan Chicken and seek genetic markets of chicken reproduction trait. [Method] The correlations between TGFβ3 gene and reproduction traits are determined by analyzing 60 Liboyaoshan bens based on PCR technology. [Result]There are 17 polymorphic sites in TGFβ3 gene of Liboyaoshan Chicken. 53 (T→C ) , 1653 ( C→T) and 1755( A→G) sites are related to total egg number from 120 to 300 d age (EN300) , age at first broodiness behavior (AFBB) , duration of age at first bloodiness behavior ( DAFBB) , interval of age at first broodiness behavior between anew laying (IAFBBA) and times of broodiness behavior from 120 to 300 d of age (TBB300) significantly or very significantly. 3343 (C→T) site is related to AFBB, IAFBBA and TBB300 significantly or very significantly. 3540( C→T) site is related to AFE, EN300 and DAFBB signifi-cantly or veiy significantly. 4786(C→T) and 7471 (G→A)site is significantly related to EN300 and DAFBB respectively. 7263 ( C→T) site is related to DAFBB significantly. [Conclusion] 53(T→C) , 1653( C→T) , 1755( A→G) , 3343 ( C→T) , 3540( C→T) , 4786( C→ T) , 7263 ( C→T) and 7471 ( G→A)sites of TGFβ3 gene are significantly related to reproduction traits, which indicates that diese polymorphic sites can be used as assistant selection markers for improvement of reproductive capacity of Liboyaoshan Chicken. [ABSTRACT FROM AUTHOR]

【Objective】 The stydy aims to analyze the associations of gene POU1F1 with chicken egg laying traits and provide references for selection breeding of Libo Yaoshan chicken. 【Method】 Six primers were designed and eight polymorphic sites were selected，and the association analysis was performed in 60 chickens of Libo Yaoshan female line by PCR-sequencing methods. 【Result】58 (A→G) mutation sites were significantly associated with the total egg number from 120 to 300 d of age (EN300)， age at first broodiness behavior (AFBB) and times of broodiness behavior from 120 to 300 d of age (TBB300) (P <0. 05 or P < 0. 01). 65 (T→C) mutation sites were significantly associated witli EN300，AFBB and TBB300 (P < 0 .05). 11041 (T→C) mutation sites were significantly associated witli EN300. Duration of age at first broodiness behavior ( DAFBB) and interval of age at first broodiness behavior between anew laying (IAFBBA) (P < 0. 05 or P < 0. 01). 【Conclusion】58 (A→G) and 65 (T→C) may be regarded as an effective molecular marker on the MAS( Marker Assisted Selection) program for EN300，AFBB and TBB300 ； 11041 (T→ C) may be regarded as an effective molecular marker for EN300，DAFBB and IAFBBA. [ABSTRACT FROM AUTHOR]

【Objective】 The study aims to provide references for genetic improvement and assistant breeding of reproduction traits of Yaoshan Chicken.【Method】 The genetic diversity of gene STAT5 was detected by DNA sequencing techniques, and an association analysis was conducted on polymorphic sites and reproduction traits.【Result】The mutation sites (11229，11298，10275,6571 T→C and 6669,6732,6898, 7142,7211,7219,7260 G→A)were found in amplified fragments of gene STAT5. The site 6898 G→A has the highest heterozygosity and content of information in a polypeptide, the site 7260 G→A has the lowest ones, respectively 0.0950 and 0.0905. All the mutation sites were at intermediate polymorphic status,but the site 6571 T→C,6732 G→A and 7260 G→A. The association of polymorphisms revealed that 6571 T→C and 7260 G→A had no significant correlation with reproduction traits of Yaoshan chicken, the others all had significant (P<0.05) or highly significant(P<0.01) correlation with reproduction traits.【Conclusion 】Polymorphism of gene StaT5 in Yaoshan Chicken had some correlation with reproduction traits, which would lay a foundation for high-yield chicken selection through molecular breeding. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Libo Yaoshan Chicken, a Guizhou local chicken variety, was used to detect polymorphic site of five DA receptor genes(DRD1-DRD5) by PCR sequencing and the relationship between these DA receptor genes and chicken reproductive traits was analyzed to discuss the relationship between Dopamine(DA) receptor gene and chicken reproductive traits and find out the important markers for chicken reproductive traits. Results: 1073(G→A) of DRD1 gene is related to egg number of 300 days(EN300) and age at first broodiness behavior(AFBB) very significantly.More than 15 polymorphic sites of DRD2 gene are related to chicken reproductive traits significantly or very significantly.14 sites of 15 sites distribute to different introns. The site of 4260(G→C) is located in exon-6, which is related to age of first egg(AFE), EN300 and AFBB significantly. 11720(C→G) of DRD3 gene is related to EN300 significantly.The individuals with CC type and11720(C→G) are no broodiness behavior. 5365(C→T) of DRD4 gene is related to AFBB significantly and 5546(G→A) of DRD4 gene is related to AFBB and duration of age at first broodiness behaviour(DAFBB) significantly. The above polymorphic sites are used as the assistant selection markers to influence the related reproductive traits significantly or very significantly. [ABSTRACT FROM AUTHOR]

In order to explore the effects of POMC in food intake and energy balance of animals, Genetic variation of POMC was detected by DNA sequencing techniques in 92 individuals from Guanling cattle, and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Guanling cattle. Results: five mutation sites(138 C→T, 430 C→T, 644 C→T, 668C→T, 693A→G) were found in 870 bp amplified fragments. χ2 test showed that the five mutation sites were all in HWE( P ﹥ 0. 05) but 430 C→T, Analysis of genetic diversity indicated that the five mutation sites were at intermediate polymorphic status. The association of polymorphisms revealed that 644 C→T and 668C→T had significant correlation with body length of Guanling cattle( P ﹤ 0.05). [ABSTRACT FROM AUTHOR]