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目的 研究BAT评分联合CTA点征对幕上自发性脑出血患者早期血肿扩大的预测价值。 方法 回顾性分析2021年9月—2022年4月连续于首都医科大学附属北京天坛医院急诊就诊且发病至一站式CT检查时间在6 h内的幕上自发性脑出血患者的资料，根据发病后24～48 h复查头颅CT与基线CT的血肿体积对比，分为血肿扩大组和非血肿扩大组。比较两组患者的基本临床资料、CTA点征及非增强CT（non-contrast computed tomography sign，NCCT）特点（低密度征、混合征等）影像资料、BAT评分（blend sign，any hypodensity，timing of NCCT score，BAT）。采用单因素分析和多因素logistic回归分析血肿扩大的危险因素，并绘制ROC曲线分析BAT评分、CTA点征及两者结合对自发性脑出血患者早期血肿扩大的预测价值。 结果 共纳入97例患者，血肿扩大组28例，非血肿扩大组69例。血肿扩大组患者CTA点征，NCCT低密度征、混合征的发生率均高于非血肿扩大组，BAT评分中位数和BAT评分≥3分的患者比例均高于非血肿扩大组，发病至基线影像时间短于非血肿扩大组，基线出血体积和基线随机血糖水平高于非血肿扩大组，上述差异均有统计学意义。多因素logistic回归分析结果显示，CTA点征阳性（OR 31.828，95%CI 5.350～189.337，P＜0.01）、BAT评分≥3分（OR 71.976，95%CI 5.391～960.899，P＜0.01）、基线出血体积（OR 1.029，95%CI 1.003～1.055，P＝0.03）和基线随机血糖（OR 1.355，95%CI 1.070～1.714，P＝0.01）可独立预测血肿扩大。ROC曲线分析显示，基线出血体积预测血肿扩大的AUC为0.762，BAT评分≥3分预测血肿扩大的AUC为0.716，CTA点征阳性预测血肿扩大的AUC为0.756，BAT评分≥3分联合CTA点征阳性预测血肿扩大的AUC为0.833。De Long检验提示两者联合预测效力优于单独预测效力（P＜0.05）。 结论 幕上自发性脑出血患者发病6 h内，BAT评分≥3分及CTA点征阳性都可有效预测血肿扩大，两者联合预测的效力更佳。 Abstract: Objective To study the predictive value of the blend sign, any hypodensity, timing of NCCT (BAT) score combined with CTA spot sign for early hematoma expansion (HE) in patients with spontaneous supratentorial intracerebral hemorrhage (sICH). Methods A retrospective analysis was conducted on sICH patients who were admitted to the emergency department of Beijing Tiantan Hospital, Capital Medical University from September 2021 to April 2022 with a time from onset to one-stop CT within 6 hours. The patients were divided into HE group and non-HE group based on the comparison of hematoma volume between baseline CT and the follow-up CT done within 24-48 hours after onset. The clinical characteristics, CTA spot sign, non-contrast computed tomography sign (NCCT) (including the hypodensity sign, blend sign, etc) , and BAT score were identified and compared between 2 groups. Univariate analysis and multivariate logistic regression analysis was used to analyze the risk factors affecting the HE, and receiver operating characteristic was drawn to analyze the predictive value of BAT score, CTA spot sign and their combination on hematoma expansion in patients with sICH. Results A total of 97 patients, including 28 in the HE group and 69 in the non-HE group. The incidence of CTA spot sign, NCCT hypodensity sign, and blend sign in patients with HE group was higher than that in non-HE group. The median BAT score and the proportion of patients with BAT score≥3 were higher than those in non-HE group. The time from onset to baseline imaging was shorter than that in non-HE group. Baseline hematoma volume and baseline random blood glucose levels were higher than those in non-HE group. The above differences were statistically significant. The results of multivariate logistic regression analysis showed that positive CTA spot sign (OR 31.828, 95% CI 5.350-189.337, P

近年来综合能源服务在理论、技术和业态上不断发展，而“双碳”目标对中国综合能源服务提出了新的要求和挑战。因此，“双碳”目标下综合能源服务的发展态势及理论体系需要进一步辨识。首先，通过梳理中国综合能源服务的发展态势及其业态特征，揭示了企业数量与国家碳排放强度及省域GDP呈现较高的相关性；其次，从“物理、信息、价值”的维度，刻画了综合能源服务“三元驱动”的体系架构、理论框架及关键技术，以完善综合能源服务的概念模型；最后，针对一些综合能源服务项目经济效益欠佳的问题，在宏观政策分析的同时，从产业生态、理论技术及增值服务方面对“双碳”目标下中国综合能源服务的发展提出建议。

目的探讨伴有酸中毒的家族性低钾型周期性瘫痪的诊断和治疗。方法分析先证者病史、临床表现、实验室检查和影像学特点，详细调查家系患病情况，应用二代测序技术检测低钾血症相关致病基因位点，对国内外相关文献进行归纳总结。结果明确诊断先证者为家族性低钾型周期性瘫痪，先证者的SCN4A基因有1个杂合突变，为c.2006G>A，导致氨基酸改变p.R669H。先证者的祖父、父亲和伯伯存在相同变异。结论伴酸中毒的家族性低钾型周期性瘫痪罕见，容易误诊为肾小管酸中毒，临床上要提高警惕。SCN4A基因c.2006G>A突变是该家系发病的分子基础。不同基因突变的临床表型存在差异，基因筛查有助诊断分型和治疗方案选择。

多能互补作为能源消费、能源供给、能源技术和能源体制革命中的关键点，是进一步提升可再生能源消纳和能效的重要途径。㶲是能量系统效率的表征，能够更加深刻地揭示系统用能品质损失的大小、部位和影响因素，但是当前国内针对多能互补能源系统进行㶲分析的工作较少。为从㶲的角度对多能互补能源系统开展分析，建立系统的水的物质平衡约束和热力模型，同时构建系统㶲分析的黑箱模型，从而将㶲分析的评价指标作为系统运行的目标函数之一。结合某园区能源系统实例，分析分别以运行成本最低、㶲效率最大为目标函数时，系统在供暖期的调度策略，为多能互补能源系统项目提供参考。算例结果表明，系统㶲效率提升了17.8个百分点，付出的代价则是运行成本增加了30.5%。

随着中国电动汽车的快速发展，大批量动力电池不断退役，这为降低储能投资成本带来机遇，但退役电池再利用于电力储能后的性能变化规律尚无确定性结论。以青岛薛家岛电动公交车充换电站退役电池为研究对象，将支持向量回归机和粒子滤波方法应用到电池容量预测中，并在预测过程中结合小波变换和电池外特性对电池的容量突变进行辨识，提出了基于抽检试验的退役电池再利用寿命预测方法，并进行了验证。验证结果表明，该方法可以提高容量衰退路径的预测准确度，同时降低退役电池容量突变对预测精度的影响，这对退役动力电池的梯次利用具有现实意义。

非洲地区新能源资源丰富，具备建设大型可再生能源发电基地的潜力。非欧联网输送可再生能源，可以在更大范围内实现清洁能源开发的最优经济性和优化配置。本文综合考虑政策措施等外部影响因素，提出一种改进的新能源发电技术经济性分析模型（CORT），并利用该模型测算了未来建设非洲大型可再生能源基地和非欧联网输电的经济性。研究表明，非洲风电联网外送的经济性不明显，太阳能发电外送具备一定的经济性，非欧联网水光打捆输送更具经济性。

文章提出一种多阶梯度链路抽象方法以及在多域路由中辅助此方法来对连接建立请求进行判断的处理模式。使用该链路抽象方法以及辅助处理模式可以近似无差错地体现原始信息,为多域路由提供准确的信息。仿真分析表明,使用多阶梯度链路抽象方法和辅助处理,可以大大减小连接建立请求的误接受和误拒绝的概率,提高网络资源利用率和网络整体性能。

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chemical Engineering (China) / Huaxue Gongcheng is the property of Hualu Engineering Science & Technology Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the Rap 1/PI3K/AKT signaling pathway in inducing apoptosis of mouse spermatocytes (GC 一 2 spd) induced by tris (1,3-dichloro -2 - propyl) phosphate (TDCIPP) the role of death. Methods GC - 2 spd cells were treated with 0, 15, 30, and 60(xmol/L TDCIPP for 24 hours. Using flow cytometry to detect cell expression levels, Western blotting to examine the expression levels of Rapl, PI3K, AKT, phosphorylated AKT (p - AKT) proteins, as well as related proteins cleared PARP and cleared Caspase - 3 in cells, and apoptosis - related proteins cleaved PARP and cleaved Caspase - 3 in the cells. ESI09 was used to inhibit the expression of Rapl protein in GC -- 2 spd cells, followed by exposure to TDCIPP. Western blotting and flow cytometry were used to respectively assess the protein levels of PI3K, AKT, p 一 AKT, cleaved PARP, and cleaved Caspase - 3, as well as the level of cell apoptosis. Results Compared to the control group, the cell apoptosis and expression of cleaved PARP and cleaved Caspase 一 3 proteins were significantly increased in the TDCIPP 一 exposed cells (P < 0. 01), The expression level of Rapl was significantly increased (P < 0. 01), while the expression levels of PI3K, AKT, and p - AKT were decreased (P <0. 01). Compared to the TDCIPP 一 exposed group, the expression of Rapl was down - regulated (P < 0. 01), while the expression levels of PI3K, AKT, and p 一 AKT proteins were up 一 regulated (P < 0.05) in the cells co 一 treated with ESI09 and TDCIPP. Meanwhile, the expression levels of cleaved PARP and cleaved Caspase -3 in the cells, as well as the level of cell apoptosis, were significantly reduced (P < 0. 05). Conclusion TDCIPP induces overexpression of Rapl protein and obstructs PI3K/AKT signaling transduction, eventually leading to apoptosis of mouse spermatocytes. [ABSTRACT FROM AUTHOR]

目的　通过根骨系统（IRS）建立三维牙颌模型，观察成人口腔正畸患者无托槽隐形矫治过程中牙根的移 动情况。方法　收集使用无托槽隐形矫治器进行正畸治疗的成人患者7例，共计164颗牙作为研究对象。在矫治前 及矫治中期对患者进行口内扫描及锥形束CT拍摄，通过IRS进行三维牙颌模型重建，并将重建的矫治前牙颌模型、 矫治中期实际牙颌模型以及矫治中期设计模拟牙颌模型导出，采用Geomagic Studio软件进行模型配准。以矫治前 的牙齿作为参考模型对每颗牙齿建立三维坐标系，比较牙根在三维方向（颊舌向、近远中向、垂直向）上的实际移动 量与模拟移动量，计算并比较牙根在三维方向上的移动效率；进一步将牙根移动方向细分为颊向、舌向、近中向、远 中向、压低向、伸长向，比较不同方向牙根的实际移动量与模拟移动量及移动效率。结果　牙根在颊舌向、近远中 向、垂直向及颊向、舌向、近中向、远中向、压低向、伸长向上的模拟移动量均高于实际移动量（P均<0. 05）。牙根移 动效率近远中向高于颊舌向（P<0. 05），垂直向与颊舌向、近远中向牙根移动效率比较差异均无统计学意义；牙根移 动效率颊向高于舌向，压低向高于伸长向（P 均<0. 05），远中向牙根移动效率与近中向比较差异无统计学意义 （P>0. 05）。结论　成人口腔正畸患者无托槽隐形矫治过程中牙根在三维方向上的实际移动量均低于模拟移动量， 牙根在舌向及伸长向的移动较为困难，建议对舌向及伸长向进行过矫治设计以辅助牙根移动。 [ABSTRACT FROM AUTHOR]

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the potential causal relationship between oily nut consumption and primary lung cancer by two-sample Mendelian randomization method.Methods Single nucleotide polymorp hisms (P< 5×10-7) associated with oily nut intake were screened from the openGWAS public database of genome - wide association study as instrumental variables. The inverse variance weighting method was the main analysis method to evaluate the causal effect of this study, and MR-Egger regression and weighted median method were used to supplement the explanation. OR value and 95%CI were used to evaluate whether there was a causal relationship between oily nut intake and primary lung cancer. The mr_pleiotropy_test and MR-PRESSO were used to test the level pleiotropy, and the leave-one-out method was used to test the sensitivity. Results A total of 100 SNPs were extracted from the ukb-b-16 576 dataset as instrumental variables. The MR-Egger regression, weighted median, and inverse variance weighting methods yielded OR values and 95% confidence intervals [0.63, 95%CI (0.11- 3.65), P = 0.611], [0.34, 95%CI (0.19-0.61), P = 0.001], [0.32, 95%CI (0.21-0.48), P = 0.001], rerespectively. The mr_pleiotropy_test showed that the MR-Egger intercept was close to 0 (-7.66E-03) with a p- value of 0.441, indicating no significant horizontal pleiotropy. Similarly, the MR-PRESSO package GlobalTest yielded a p-value of 0.162, further confirming the absence of horizontal pleiotropy among the selected instrumental variables.After conducting the leave-one-out sensitivity analysis, no significant influence on the results was observed from any specific SNP locus, indicating that the results are highly stable and reliable. Conclusions The two-sample Mendelian randomization analysis revealed a negative causal relationship between oily nut intake and the risk of primary lung cancer. However, further verification is needed with larger sample sizes and data from individuals of different ethnic backgrounds to strengthen the findings. [ABSTRACT FROM AUTHOR]

Copyright of Yingyong Shengtai Xuebao is the property of Chinese Journal of Applied Ecology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of New Building Materials / Xinxing Jianzhu Cailiao is the property of New Building Materials Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Fly Ash Comprehensive Utilization is the property of Hebei Fly Ash Comprehensive Utilization Magazine Co., Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Geodesy & Geodynamics (1671-5942) is the property of Editorial Board Journal of Geodesy & Geodynamics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Arid Zone Research / Ganhanqu Yanjiu is the property of Arid Zone Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chemical Engineering of Oil & Gas / Shi You Yu Tian Ran Qi Hua Gong is the property of PetroChina Southwest Oil & Gas Field Company and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Silk is the property of Zhejiang Sci-Tech University Magazines and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Clinical Hepatology / Linchuang Gandanbing Zazhi is the property of Journal of Clinical Hepatology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: In recent years, there have been many studies on the mechanism of exosomal non-coding RNA in gestational diabetes mellitus, but there is a lack of the latest systematic review of exosomes from different sources, especially placental sources. OBJECTIVE: To summarize the changes and potential roles of microRNA (miRNA), long non-coding RNA (lncRNA), circular RNA (circRNA), and exosomes in gestational diabetes mellitus to provide potential targets for early screening and treatment of clinical gestational diabetes mellitus. METHODS: A literature search was conducted on PubMed, Web of Science, China National Knowledge Infrastructure, WanFang Data, and VIP databases to retrieve relevant articles on non-coding RNA or exosomal non-coding RNA in relation to gestational diabetes mellitus. A total of 74 articles were included for review. RESULTS AND CONCLUSION: (1) Non-coding RNAs play important pathological and physiological roles in the lifecycle activities, and increasing evidences suggest that non-coding RNAs are involved in the occurrence and development of gestational diabetes mellitus by regulating various physiological functions. This provides a new direction for the research of gestational diabetes mellitus. (2) Exosomes are widely present in the human body. Various cells can secrete exosomes, such as red blood cells, epithelial cells, and placental cells. Non-coding RNAs found in exosomes from different sources have been demonstrated to play a role in the pathogenesis, diagnosis, and treatment of gestational diabetes mellitus. (3) MiRNA and gestational diabetes mellitus: The role of peripheral blood miRNA in gestational diabetes mellitus is mainly to affect the functions of trophoblast cells, pancreatic beta cells and blood glucose levels in gestational diabetes mellitus; placental miRNA can reflect the severity of gestational diabetes and impair the function of trophoblast cells. (4) LncRNA and gestational diabetes mellitus: Peripheral blood lncRNA can induce insulin resistance through the phosphatidylinositol 3-kinase/protein kinase B pathway and may provide new insights for the diagnosis and treatment of gestational diabetes mellitus; placental lncRNA can regulate proliferation and migration of placental trophoblast cells, promoting the occurrence and development of gestational diabetes mellitus. (5) CircRNA and gestational diabetes mellitus: Peripheral blood and placental circRNA can induce the occurrence and development of gestational diabetes mellitus by impairing the proliferation, migration and metabolism of placental trophoblast cells. (6) Non-coding RNA in exosomes and gestational diabetes mellitus: Peripheral blood non-coding RNA in exosomes can affect gestational diabetes mellitus blood glucose levels and glucose homeostasis, and participate in the occurrence and development of gestational diabetes mellitus by influencing placental function. (7) Non-coding RNA has the potential to serve as biomarkers for early diagnosis of gestational diabetes mellitus. Additionally, engineered exosomes can better achieve targeted therapy for gestational diabetes mellitus. These latest findings provide a reference for both basic research and clinical translation of gestational diabetes mellitus. (8) In the future, improvements in the extraction and purification methods of peripheral blood exosomes should be improved, and factors such as race, diet and physical activity should be excluded to improve the reproducibility of results. Further prospective clinical studies are required to explore the clinical application of circulating non-coding RNA and exosomes in the prediction and diagnosis of gestational diabetes mellitus. [ABSTRACT FROM AUTHOR]

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目的 观察含白蛋白结合型紫杉醇化疗方案化疗过程中联合口服神效黄芪汤加减方和甲钴胺的乳腺癌 患者周围神经毒性 (CIPN)发生情况, 为神效黄芪汤加减方在CIPN防治中的应用提供参考。方法 收集应用含白蛋白 结合型紫杉醇方案化疗至少6个周期的初治乳腺癌患者90例, 随机分为实验组和对照组, 各45例。自接受含白蛋白 结合型紫杉醇化疗时, 实验组开始口服神效黄芪汤加减方 (黄芪 40 g、人参 5 g、桂枝 10 g、防风 6 g 、白芍 15g、鸡血藤 30 g、鸡矢藤30 g、枳壳10 g、炙甘草6 g)联合甲钴胺片 (0. 5 mg/次, 每天3次), 对照组患者开始口服甲钴胺片, 用法同 实验组。6周期化疗结束时, 比较两组CIPN的发生率。化疗第1、2、3、4、5、6周期结束时, 比较两组周围神经病变程度 [周围神经病变分级 (NCI-CTCAE)、生活质量［体能状态评分 (KPS)]、药物安全性相关指标 [血常规 (白细胞计数、血 红蛋白、血小板计数)及肝功能 (AST、ALT、直接胆红素)]。结果 共入组90例患者, 完成88例, 脱落2例 (均为对照 组, 于第一周期化疗后因个人原因脱离)。与对照组比较, 实验组CIPN的发生率低 (P<0. 05)。前4个化疗周期, 两组 NCI-CTCAE评分比较, P均>0. 05；第5、6化疗周期, 两组NCI-CTCAE评分比较, P均<0. 05。两组KPS评分比较, P均> 0. 05。两组肝肾功能、血常规比较, P均>0. 05。结论 白蛋白结合型紫杉醇化疗方案化疗过程中联合口服神效黄芪 汤加减方和甲钴胺的乳腺癌患者CIPN发生率低于单用甲钴胺；与单用甲钴胺比较, 神效黄芪汤加减方联合甲钴胺口 服对乳腺癌含白蛋白结合型紫杉醇化疗方案所致CIPN的防治作用更优, 可降低CIPN的发生率、减轻CIPN程度。 [ABSTRACT FROM AUTHOR]

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Objective: To clone the Helicobacter pylori (Hp) hp0169 gene and conduct the crystallographic study, and to clarify its secondary and tertiary structures. Methods: The hp0169 gene and its encoded protein sequence of the Hp NCTC26695 strain were retrieved from the UniProt database. Bioinformatics method was used to analyze the physicochemical properties of the Hp recombinant protease (HpPrtC) protein; SOPMA and DNAStrar softwares were used to predict the secondary structure characteristics of HpPrtC protein; SWISS-MODEL software was used to construct the tertiary structure of the HpPrtC protein; IEDB and ABCpred softwares were used to predict the antigenic epitopes of the B lymphocytes HpPrtC protein; SYFPEITMI website was used to predict the antigenic epitopes of the T lymphocytes of HpPrtC protein; the expert pool (EP) and random forest (RF) algorithms were used to predict the crystallizability of the HpPrtC protein; the HpPrtC recombinant protein was expressed in the prokaryotic system; the HpPrtC recombinant protein was purified by Ni2+ affinity chromatography and size-exclusion chromatography; the crystallization conditions for HpPrtC were screened by crystallization kit. Results: The hp0169 gene contained 1 269 base pairs and encoded the protein of 422 amino acids, the theoretical isoelectric point was 7. 64 and the relative molecular weight was 47 300. HpPrtC was a hydrophilic and soluble protein. The number of amino acids of alpha helices of HpPrtC accounted for 35. 78%, beta sheets 18. 72%, beta turns 6. 87%, and random coils 38. 63%. The antigen epitope analysis results showed that HpPrtC contained five dominant linear epitopes of B lymphocytes, three conformational epitopes, and multiple potential dominant epitopes of T lymphocytes. The homology modeling results showed that HpPrtC formed a dimer, and each monomer displayed a barrel structure surrounded by β sheets, alpha helices, and random coils. HpPrtC was predicted to have moderate crystallizability without signal peptides and transmembrane helices. Small clustered needle-like crystals of HpPrtC were obtained under the conditions of 0. 2 mol·L-1 magnesium chloride, 0. 1 mol·L-1 tris (hydroxymethyl) amino methane (Tris), 3. 4 mol·L-1 hexanediol, and pH=8. 5. Conclusion: HpPrtC is a hydrophilic protein that forms a dimeric structure and crystallizes into small clustered needle-like crystals under suitable conditions. HpPrtC contains dominant antigenic epitopes of the T lymphocytes and B lymphocytes and can serve as an antigen for the design of Hp vaccines to establish the multivalent fusion vaccines or multi-epitope vaccines; the results provide an experimental basis for the prevention and control of Hp. [ABSTRACT FROM AUTHOR]

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Objective To compare the repair effects of focused low-intensity pulse ultrasound (FLIPUS) combined with local injection of platelet-rich plasma (PRP) on facial nerve injury in rats. Methods Twenty healthy male SD rats were randomly divided into the model group, PRP group, FLIPUS group, and PRP+FLIPUS group, with 5 rats in each group. We established the right lateral facial nerve injury models in each group, and rats in the PRP group received local injection of 50 µL PRP at the nerve injury site, twice a week； rats in the FLIPUS group received FLIPUS treatment at the site of nerve injury for 10 min, once per day； rats in the PRP+FLIPUS group were treated with local injection of PRP and FLIPUS according to the above method； rats in the model group were not subject to special treatment； and the treatment period for each group was 28 d. Behavioral assessments were conducted weekly after surgery in each group, and scores of beard movement and blink reflex were recorded. Before and on the 28th day after surgery, right lateral nerve electrophysiological examination was performed using an electromyography evoked potential device in each group. The maximum CMAP amplitude of the injured facial nerve was recorded and the R value was calculated. After rats of each group were euthanized, the injured facial nerve segment tissues were taken and stained with HE for histopathological observation. At the same time, the average number, diameter, and area of axons per unit area were recorded. Results There were no significant differences in the blink reflex or beard movement scores in rats at 1-4 weeks after surgery between groups (all P> 0. 05). Compared with the same group before surgery, the maximum CMAP amplitude of facial nerve injury in rats of each group decreased after surgery (P<0. 05), and there were no significant differences among all groups after operation (all P> 0. 05). Compared with the model group, the R values of the injured facial nerves in the PRP group, FLIPUS group, and PRP+FLIPUS group all increased (all P<0. 05), while there was no statistically significant difference between the PRP group, FLIPUS group, and PRP+FLIPUS group (all P>0. 05). Compared with the model group, the PRP group, FLIPUS group, and PRP+FLIPUS group showed reduced degree of nerve bundle degeneration, demyelination, and axonal vacuolization, increased number of regenerated axons, and more orderly arrangement of nerve fibers. Compared with the model group, the number of axons per unit area in the injured facial nerve segment tissue of rats in the PRP group, FLIPUS group, and PRP+FLIPUS group increased (all P<0. 05）； the axon diameter and area per unit area of the injured facial nerve segment tissue in the PRP group rats were higher than those in the model group, FLIPUS group, and PRP+FLIPUS group (all P<0. 05). Conclusion The repair effect of FLIPUS combined with local injection of PRP on facial nerve injury in rats is not superior to that of FLIPU and PRP alone. [ABSTRACT FROM AUTHOR]

To investigate the effects of honokiol on proliferation and apoptosis of human adipose-derived mesenchymal stem cells(hADSCs), and to investigate the effect of the drug on the tumor microenvironment. Methods hADSCs were incubated with different concentrations of honokiol, the proliferation of hADSCs was detected by MTS and Trypan blue staining, and cell apoptosis was assessed by annexin V/PI double staining. In the meantime, expression of mRNA and protein related to cell proliferation and apoptosis were detected by qPCR and Western blot, respectively. The expression of total MEK, phosphorylated MEK, total ERK and phosphorylated ERK proteins in the MEK-ERK1/2 signaling pathway were detected by Western blot. Results The effect of honokiol on inhibiting proliferation and promoting apoptosis of hADSCs was significantly enhanced with the increase of concentration. The expressions of proliferation-related genes CCND1, MKI67 and PCNA were down-regulated. The expressions of pro-apoptotic genes BAX and TP53 was up-regulated, and the expressions of anti-apoptotic gene BCL2 was down-regulated. Honokiol inhibited MEK and ERK1/2 phosphorylation in a concentration-dependent manner. Conclusions Honokiol inhibits proliferation and promotes apoptosis of hADSCs, and the specific mechanism is potentially related to the inhibition of MEK-ERK1/2 pathway. [ABSTRACT FROM AUTHOR]

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Copyright of Chinese Journal of Inorganic Analytical Chemistry / Zhongguo Wuji Fenxi Huaxue is the property of Beijing Research Institute of Mining & Metallurgy Technology Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Arid Land Geography is the property of Chinese Academy of Sciences, Xinjiang Institute of Ecology & Geography and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)