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Copyright of Journal of Xi'an Jiaotong University (Medical Sciences) is the property of Xian Jiaotong University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Xi'an Jiaotong University (Medical Sciences) is the property of Xian Jiaotong University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Xi'an Jiaotong University (Medical Sciences) is the property of Xian Jiaotong University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the important role of Gli-1 in hypoxia-induced epithelial-mesenchymal transition of breast cancer cell line MDA-MB-231. Methods For the present study, breast cancer cell line MDA-MB-231 was cultured under normoxic or hypoxic condition. Then, the shRNA of Gli-1 was stably transfected into MDA-MB-231 cells. Invasion of MDA-MB-231 was detected by the Transwell assay. The expressions of HIF-1α, Gli-1, E-Cadherin and vimentin proteins were determined by Western blot. Results Hypoxia significantly induced the invasion of MDA-MB-231 cells, upregulated the expressions of HIF-1α, Gli-1 and vimentin proteins, and downregulated the expression of E-Cadherin protein. Furthermore, knocking down Gli-1 obviously abrogated hypoxia-induced epithelial-mesenchymal transition and enhanced invasion of MDA-MB-231 cells. Conclusion Hypoxia induces epithelial-mesenchymal transition and enhances invasion of breast carcinoma cells via strengthening Gli-1 expression and activation of Hedgehog pathway. [ABSTRACT FROM AUTHOR]