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Objective To investigate the potential mechanism of osteoking in the treatment of type 2 diabetic osteoporosis by inhibiting osteoblast ferroptosis from the perspective of network pharmacology and molecular docking.Methods TCMSP database and BATMAN-TCM database were used to retrieve the components and targets of traditional Chinese medicine, and GeneCards and OMIM database were used to obtain the disease targets of osteoporosis in type 2 diabetes.The PPI protein interaction network was constructed with STRING11.0 database, and topological analysis was performed to screen important targets in the network. The potential therapeutic targets were imported into the DAVID online database for gene ontology(GO) enrichment analysis and gene and genome encyclopedia(KEGG) pathway enrichment analysis.Finally, molecular docking simulation was performed to verify the results of network pharmacology.Results A total of 223 potential therapeutic targets were obtained through network pharmacology screening.The key active substances of osteoking in the treatment of type 2 diabetic osteoporosis are luteolin, 20(R)-ginsenoside Rh2, kaempferol, baicalein, beta-sitosterol, etc.The key targets are IL-6, Akt, TNF, MAPK3, HIF-1A, etc.The results of GO and KEGG analysis showed that the treatment of osteoporosis in type 2 diabetes by osteoking mainly included biological processes such as lipopolysaccharide response, hypoxia response, and inflammatory response, and regulated HIF-1A, TNF, IL-17, PI3K/Akt and other signaling pathways.The results of molecular docking showed that 7-O-methylisoprostol, baicalein, kaempferol, and quercetin had good binding activity with HIF-1A.Conclusion Through network pharmacology and molecular docking technology, it is found that osteoking may treat type 2 diabetic osteoporosis through multiple targets, and may reduce oxidative stress and inhibit osteoblast ferroptosis through HIF-1A signaling pathway to treat type 2 diabetic osteoporosis. [ABSTRACT FROM AUTHOR]

The elasticity of the distribution network indicates the ability of the system to restore its original state after interference, and the frequent occurrence of natural disasters requires that the distribution network can not only operate reliably under normal conditions, and but also maintain the necessary capabilities for extreme disasters. Accordingly, a reliability evaluation method of elastic distribution network based on fault incidence matrix(FIM)was proposed in this paper. With FIM, the explicit analytical calculation of the reliability, elasticity and power supply capacity of the distribution system can be achieved by using simplified matrix algebra and Boolean operation, which saves the repetitive search work of fault enumeration in the evaluation process, and the calculation time, and can be applied to the complex structure of the distribution network and easy to expand. The final results show that the calculation time of the proposed method is only about 0.055s, which is reduced by 87% and 75% respectively compared with that of the fault diffusion algorithm and the block algorithm. [ABSTRACT FROM AUTHOR]

[Objective] The aims of this study are to accurately predict the peak flow at small watershed scale, and to provide support for hydrological application and soil and water conservation assessment. [Methods] The data of 215 rainfall events from 2013 to 2021 were used to analyze effect factors of the peak flow in Liujiagou small watershed, Wanzhou County, Chongqing. The Pearson correlation analysis was performed between peak flow rate and effect factors, including runoff depth, runoff coefficient, rainfall depth, maximum 30-minute rainfall intensity(I30), and average rainfall intensity. A new peak flow rate equation was developed based on linear regression and nonlinear regression analysis to predict the peak flow rate for small watersheds in Chongqing. The first data set, including 172 storms, was used to develop a new formula for predicting peak flow rate. A second set of data, including 43 storm events, was used to test the new equation. [Results] The peak flow rate was significantly correlated with rainfall, I30, runoff depth, and runoff coefficient at the 0.01 confidence level. The model efficiency was 0.85 and 0.82 when 172 and 43 rainfall events were used, respectively. [Conclusion] The new equation can provide good accuracy in the small watershed in Chongqing. The new equation will be helpful for the decision-making of soil and water conservation measures, the design of soil and water conservation engineering facilities and the evaluation of soil erosion in Chongqing and even the small watersheds in southwest China. [ABSTRACT FROM AUTHOR]

Objective：To observe whether loss of controllable stress induces learning and memory impairment and to explore the mechanism of acupuncture in the treatment of learning and memory impairment. Method：We established the mouse model of loss of controllable stress on the basis of the classical animal model of depression-learned helplessness model，that is，mice can actively avoid foot shock by learning to control the effective nasal tentacles in Skinner's box in the first 3 days，while the function of effective nasal tentacles was lost in the last 3 days and the mice lost the control of foot shock. At the same time，the control group without plantar electric shock was designed. After modeling， the changes of learning and memory behavior were detected by new object recognition box and Morris water maze， and the improvement of learning and memory was also observed after electroacupuncture at auricular points. Golgi staining was used to observe the structural changes of ventral prefrontal cortex neurons. Immunohistochemical staining was used to observe the expression of C-fos in vPFC. Western Blot was used to detecte the expression of vesicular glutamate transporter I and synapsin I in type I vesicles. And the changes of corresponding indexes were also detected after acupuncture. Result：The extraction of recognition memory in the LOC group had no significant effect on the acquisition and consolidation of recognition memory，and acupuncture could improve the acquisition and consolidation of recognition memory. The glutamatergic neurons in the vPFC were significantly activated，and the expression of VGLUT1 and synaptophysin were decreased. Auditory vagus nerve stimulation could significantly reduce the activity level and regulate the expression of VGLUT1 and SYN protein. Conclusion：Auricular point stimulation can alleviate the impairment of learning and memory behavior induced by loss of controllable stress by regulating the structural changes of neurons and the expression of VGLUT1 and SYN proteins in vPFC. Author's address Innovation Center of Combination of Acupuncture and Medicine， School of Acupuncture and Massage，Shaanxi University of Traditional Chinese Medicine，Xianyang，Shanxi, 712046 [ABSTRACT FROM AUTHOR]

This paper aims to investigate the role of white-rot fungus extracellular polysaccharides in the biogenesis of iron (hydrogen) oxides. Fourier transform infrared spectroscopy (FT-IR) was used to study the characteristics of iron (hydrogen) oxides regulated by the extracellular polysaccharide of white-rot fungus under different polysaccharide concentrations, different aging time, different initial concentrations of Fe3+, different pH values of mineralization system and different aging temperature. The crystal composition and transformation between different phases of iron (hydrogen) oxides biomineralized by white-rot fungus under different conditions were investigated. The results showes that the nucleation of iron (hydrogen) oxides in polysaccharides is promoted by increasing the content of polysaccharides, and the crystal type and degree of iron (hydrogen) oxides changed during aging. The increase of the initial concentration of Fe3+ helped to improve the stability of the hydroxyl binding state of iron (hydrogen) oxides. The formation of α-FeOOH is favorable under acidic condition, while the formation of α-Fe2O3 is favorable under alkaline condition. An appropriate increase in temperature is beneficial to the improvement of Fe—O crystallinity, and a further increase in temperature favored the conversion of iron (hydrogen) oxides into a more stable phase. Those results can provide references for revealing the biological formation process of iron (hydrogen) oxides. [ABSTRACT FROM AUTHOR]

The purpose of this study is to analyze the structure and self-assembly behavior of extracellular polysaccharides produced by Phanerochaete chrysosporium BKMF-1767. The structure of extracellular polysaccharides from Phanerochaete chrysosporium is analyzed with Congo red test, molecular weight determination, X-ray diffraction and Fourier transform infrared spectroscopy, and the self-assembly behavior of the polysaccharides is studied with fluorescence microscopy, scanning electron microscopy and transmission electron microscopy. In Congo red test, the maximum absorption wavelength of the polysaccharide-Congo red solution shows obvious redshift phenomenon in low-concentration alkali solution, and the red-shift phenomenon has disappeared with the increase of alkali solution concentration, indicating that the extracellular polysaccharides from Phanerochaete chrysosporium has a triple helix structure and the structure is destroyed into disordered structure in high concentration alkali solution. During molecular weight determination, the molecular weight of the polysaccharide in dimethyl sulfoxide solution is significantly lower than that in aqueous solution, indicating that the configuration of the polysaccharide chain is depolymerized in dimethyl sulfoxide solution. Fourier transform infrared spectroscopy analysis finds that the polysaccharide has characteristic absorption peaks of polysaccharide compounds and contained β-glycosidic bonds. X-ray diffraction analysis shows that it is a kind of amorphous polysaccharide. The comprehensive analysis of fluorescence microscopy, scanning electron microscopy and transmission electron microscopy indicates that the polysaccharide can form a fibrous network-like spatial structure and form aggregates under its self-assembly behavior. The above results indicate that the extracellular polysaccharides from Phanerochaete chrysosporium is one kind of amorphous polysaccharide with triple helical structure, which can self-assemble into fibrous aggregates. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of quadratus lumborum block on inflammatory response and postoperative intestinal function recovery in patients undergoing laparoscopic colorectal cancer surgery. Methods A total of 60 patients scheduled for laparoscopic colorectal cancer surgery were randomly divided into two groups：quadratus lumborum block combined with general anesthesia group（group QA, n = 30）and sole general anesthesia group（group GA, n = 30）. The patients in group QA received bilateral quadratus lumborum block 30 minutes before induction of general anesthesia, and in group GA received simple general anesthesia, respectively. The concentration of serum TNF-α, IL-6 and IL-10 was measured at the following time points：5 minutes before anesthesia induction（T0）, at the completion of pneumoperitoneum establishment（T1）, 6 h（T2）and 24 h（T3）after operation. The time of first exhaust and first walking time after operation, the incidence and degree of abdominal distension were recorded. Results Compared with the group GA, the levels of inflammatory factors at all time points in the group QA were significantly lower（P < 0.05）. The time of first exhaust in the group QA was significantly shorter than that in the group GA（P < 0.05）, and the first walking time after operation in the group QA was significantly longer than that in the group GA（P < 0.05）. The incidence of postoperative abdominal distension in the group QA was significantly lower than that in the group GA（P < 0.05）. Conclusions Quadratus lumborum block before operation can significantly reduce the inflammatory response and promote the rapid recovery of intestinal function in patients undergoing laparoscopic colorectal cancer surgery. [ABSTRACT FROM AUTHOR]

This study accurately evaluated the lateral sealing ability of faults in the Pinghu formation in the K area of the East China Sea Basin and its influence on hydrocarbon accumulation. Based on a previous analysis of the fault lateral sealing mechanism and influencing factors, starting from the fault lateral sealing type, the evaluation model and lower threshold were established for lithologic juxtaposition type and fault rock type sealing faults, respectively, in order to evaluate the fault lateral sealing property and ability. The N-1 well block was used as an example to analyze the control effect of sealing on hydrocarbon accumulation. The results indicated that the fault lateral sealing type of the Pinghu formation in the K area is mainly of the fault rock type. The fault sealing ability is affected by the clay content of the fault rock (SGR), and the lower threshold of fault lateral sealing is 15%. In this section, the SGR value of the Pinghu formation fault rocks gradually decreases from bottom to top, the sealing ability gradually weakens, and some sealed faults gradually turn open. On the plane, the lateral sealing ability of large-scale faults with long-term activity in the central and northern areas was clearly stronger than that of the southern faults. Considering that the lateral sealing property of the fault and whether hydrocarbons can accumulate in the reservoir are the two geological factors that restrict each other, the fault is laterally sealed and the reservoir is developed only when the SGR value of the fault rock is between 15% and 58 %. Therefore, fault-related traps are conducive to hydrocarbon accumulation. These research results have high theoretical and practical significance for clarifying the law of hydrocarbon accumulation and risks of reducing drilling in the K area of the East China Sea Basin. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the mechanism of gynecological Tiaoqi Jiedu formula on chronic endometritis. METHODS: Through network pharmacology, the possible key targets and key pathways of gynecological Tiaoqi Jiedu formula on endometritis were identified. On the basis of this theory, the mouse model of endometritis was established and divided into the blank group, model group, western medicine group (levofloxacin hydrochloride), and low-dose group, medium-dose group and high-dose group (low-dose, medium-dose and high-dose gynecological Tiaoqi Jiedu formula). The network pharmacology results were verified by enzyme-linked immunosorbent assay (ELISA), real-time fluorescent quantitative polymerase chain reaction (PCR) and Western blotting. RESULTS: A total of 219 compounds and 698 corresponding targets were screened from gynecological Tiaoqi Jiedu formula. A total of 36 key targets were obtained by intersections with endometritis targets. Seventy-seven metabolic pathways were obtained by Kyoto encyclopedia of genes and genomes enrichment, including nuclear factor κB ( NF-κB) signaling pathway, advanced glycation end products (AGE)-AGE receptor signaling pathway, tumor necrosis factor signaling pathway and interleukin (IL)-17 signaling pathway. ELISA results showed that the levels of MPO, IL-6 and IL-1β in the model group were significantly higher than those in the blank group (P<0. 01). Compared with the model group, the lowdose gynecological Tiaoqi Jiedu formula group had a significant reduction in IL-6, with statistically significant difference (P<0. 05), but there was no significant difference in MPO and IL-1β between two groups (P> 0. 05). Compared with the model group, MPO, IL-6 and IL-1β in the medium-dose group, high-dose group and western medicine group decreased significantly,with statistically significant differences (P< 0. 01). The results of real-time fluorescence quantitative PCR and Western blotting showed that the gene and protein expression of P-P65 and TLR4 in the uterine tissue of the model group were significantly higher than those of the normal group, with statistically significant differences ( P < 0. 01 or P < 0. 001). Compared with the model group, the low-dose group could not significantly inhibit the expression of P-P65 and TLR4 mRNA and protein (P>0. 05), while the expression of P-P65 mRNA and TLR4 mRNA in the medium-dose group, high-dose group and western medicine group decreased significantly (P<0. 05 or P<0. 01, P<0. 001), the expression of P-P65 protein and TLR4 protein in the high-dose group and western medicine group decreased significantly (P<0. 01 or P<0. 05), the differences were statistically significant. There was no significant difference in the expression of P65 mRNA and protein in each group ( P > 0. 05). CONCLUSIONS: Gynecological Tiaoqi Jiedu formula can significantly down-regulate the levels of inflammatory factors and inhibit the expression of key genes in TLR4/ NF-κB signaling pathway in the mouse model of lipopolysaccharide (LPS)-induced endometritis, so as to achieve the purpose of anti-inflammatory and endometrial protection. [ABSTRACT FROM AUTHOR]

Objective To study the effects of body surface temperature sensory block level on the maternal body temperature, labor analgesia effect, labor progress and instrument assisted delivery during epidural labor analgesia. Methods One hundred parturients with epidural labor analgesia in this hospital from May 2021 to April 2022 were randomly divided into the high level group (T6 group) and low level group (T8 group), 50 cases in each group, and 0. 075% ropivacaine + 0. 5%μg/mL sufentanil mixture liquid was used for epidural analgesia. The T6 group: the temperature level of epidural block was controlled at T6;the T8 group: the temperature level of epidural block was controlled at T8. The effect of temperature sensory block level on maternal body temperature, as well as the influences of analgesic effect, intrapartum fever, labor process time, time from rupture of membrane to delivery, oxytocin use rate and instrument midwifery rate were observed. Results The analgesic effect in the T6 group was better than that in the T8 group, but the rate of intrapartum fever and use rate of oxytocin in the T6 group were higher than those in the T8 group, and the time of labor, time from rupture of membrane to delivery in the T6 group were longer than those in the T8 group, the differences were statistically significant (P＜0. 05). There was no statistically significant difference of the instrumental midwifery rate between the two groups (P＞0. 05). Conclusion During epidural labor analgesia, the control of temperature block level at T6 is better than at T8, but which will increase the rate of intrapartum fever, prolong the duration of labor and increase the use rate of oxytocin. [ABSTRACT FROM AUTHOR]

To observe the clinical efficacy of Yufu Jiedu ointment in the treatment of hand foot syndrome caused by capecitabine chemotherapy in patients with colorectal cancer, so as to provide a reasonable treatment plan for clinical use. From January 2021 to May 2022, 60 patients with hand foot syndrome caused by capecitabine regimen chemotherapy for colorectal cancer diagnosed in the outpatient or inpatient department of Hunan Cancer Hospital were selected. All patients were divided into the Yufu Jiedu ointment group and the urea ointment group by coin toss method, 30 patients in each group. Yufu Jiedu ointment group was treated with Yufu Jiedu ointment, urea ointment group was treated with urea ointment, and both groups were treated continuously for 21 days. The improvement of hand foot syndrome grading, clinical efficacy, TCM syndrome score, pain visual analog score (VAS), hand foot skin reaction quality of life scale (HF-QoL) score and self rating anxiety scale (SAS) score were observed in the two groups. Yufu Jiedu ointment group was superior to urea ointment group in reducing the grade of hand foot syndrome and improving the total effective rate of treatment (P<0.05). After treatment, the scores of TCM syndromes, VAS, HF-QoL and SAS in the two groups were lower than those before treatment (P<0.05), and the scores in the Yufu Jiedu ointment group were lower than those in the urea ointment group (P<0. 05). Yufu Jiedu ointment has a definite clinical effect on the treatment of hand foot syndrome caused by capecitabine chemotherapy in colorectal cancer patients. It can effectively reduce the clinical grading of patients, reduce TCM syndrome scores, relieve pain symptoms, and improve the quality of life and anxiety of patients.It has a certain clinical application value and worthy of clinical promotion and application. [ABSTRACT FROM AUTHOR]

Objective: To study the differential value of serum pepsinogen ratio (PGR), gastrin-17 (G-17), thymidine kinase 1 (TK1), rhomboid domain protein 1 (RHBDD1) combined with Helicobacter pylori antibody (HP-IgG) in atrophic gastritis and early gastric cancer. Methods: A total of 202 patients with early gastric cancer who were admitted to our hospital from January 2018 to December 2021 were selected and classified as gastric cancer group. In addition, 200 cases of chronic atrophic gastritis and 200 cases of healthy volunteers in the same period were selected and recorded as atrophic gastritis group and control group. The levels of serum pepsinogen (PG) I, PG II, PGR, G-17, TK1, RHBDD1 and the positive rate of HP-IgG were detected and compared among the three groups. The levels of serum PG I, PG II, PGR, G-17, TKI and RHBDD1 between HP-IgG positive and negative patients with early gastric cancer were compared. Receiver operating characteristic (ROC) curve was used to analyze the efficacy of the levels of serum PGR, G-17, TK1, RHB- DD1 combined with HP-IgG in the differential diagnosis of early gastric cancer and atrophic gastritis. Results: The levels of serum PG I and PGR in gastric cancer group were lower than those in atrophic gastritis group and control group, and the above indexes in atrophic gastritis group were lower than those in control group (all p<0.05). The levels of serum PG II, G-17, TK1, RHBDD1 and the positive rate of HP-IgG in gastric cancer group were higher than those in atrophic gastritis group and control group, and the above indexes in atrophic gastritis group were higher than those in control group (all P<0.05). The serum PG I and PGR of HP-IgG positive patients with early gastric cancer were lower than those of HP-IgG negative patients, while the levels of serum PG II, G-17, TK1 and RHBDD1 were higher than those of HP-IgG negative patients (all p<0.05). ROC curve analysis showed that the combination of serum PGR, G-17, TK1, RHBDD1 and HP-IgG antibody was better than the above five indicators alone in differentiating early gastric cancer from atrophic gastritis. Conclusion: The serum PGR of patients with early gastric cancer is abnormally low, while the serum G-17, TK1, RHBDD1 and HP-IgG antibodies are abnormally high. The combination of the above five indicators has a high value in differentiating early gastric cancer from atrophic gastritis. [ABSTRACT FROM AUTHOR]

Objective To explore whether the nucleic acid detection results of 11 pathogens in gastric juice and throat swab samples of newborns were consistent, so as to provide reference for the selection of pathogen nucleic acid detection samples.Methods A total of 120 newborns with suspected infection admitted to the pediatric intensive care unit of Jiangmen Central Hospital from February to June 2021 were selected as the research objects.Gastric juice and throat swab samples were collected within three hours after birth.Detection of 11 pathogens [chlamydia trachomatis（CT）,ureaplasma parvum（UP）,escherichia coli（EC）,ureaplasma urealyticum（UU）,neisseria gonorrhoeae（NG）,human herpes virus（HHV）,group B streptococcus（GBS）,cytomegalovirus（CMV）,Mycoplasma genitalium（MG）,enterovirus（EnV） and mycoplasma hominis（MH）] was completed by suspension bead liquid-phase chip for perinatal maternal and infant pathogen nucleic acid detection.The difference of pathogen detection rate between gastric juice and throat swab samples was compared, and the consistency of gastric juice and throat swab for the 11 pathogens was compared by Kappa test.Results The nucleic acid detection of pathogens in the 120 cases of gastric juice and throat swab samples showed that UP,EC,UU and MH were positive, and the other seven pathogens were not detected, including 14 cases of gastric juice samples and 12 cases of throat swab samples.There was no significant difference in the detection rates of UP,EC,UU and MH in the gastric juice and throat swab samples（P>0.05）,and the Kappa test showed good consistency（Kappa=0.848,0.483,1.000,1.000,P<0.05）.The overall results of gastric juice and throat swab samples were highly consistent（Kappa=0.673,P<0.001）.Conclusion The results of nucleic acid detection of 11 pathogens in gastric juice and throat swab samples of newborns are basically consistent. [ABSTRACT FROM AUTHOR]

Objective: To construct an insitu injectable Chitosan-based hydrogel for phased dosing, of which the sol solution is injected directly into the wound after ESD (Endoscopic Submucosal Dissection) surgery to form a hydrogel dressing in situ. After introducing drug-loaded nanoparticles into the hydrogel dressing, the dual drug-loaded hydrogel can promote the scar-free healing of wounds through different release kinetics of the drugs contained in the gel and nanoparticles, which will be a good solution for preventing post-ESD esophageal stricture. Methods: Polydopamine (PDA) is added to chitosan/sodium glycerophosphate (CS/β-GP) thermosensitive hydrogel to prepare chitosan/sodium glycerophosphate/polydopamine (CS/β-GP/PDA) hydrogel. The CS/β-GP/PDA/NPs dual drug-loaded hydrogel was prepared by adding drug-loaded polyethylene glycol-poly lactic acid-co-glycolic acid (PEG-PLGA) nanoparticles to the drug-loaded hydrogel, and the staged release of the drugs was achieved through the combination of the two drug carrier systems. The phase transition temperature and rheological properties of CS/β-GP, CS/β-GP/PDA, CS/β-GP/PDA/NPs gels were determined by rheo-logical tests. The release kinetics of the two drugs in CS/β-GP/PDA/NPs hydrogels was detected by high performance liquid chromatography (HPLC). The biocompatibility of CS/β-GP/PDA, CS/β-GP/PDA/NPs gels was evaluated by CCK-8 cell proliferation experiments. In the porcine esophagus in vitro, the wound that would generate after ESD was simulated. The sol solution was injected into the wound with the aid of endoscopy, and the state of the hydrogel was observed through an endoscope. Results: A chitosan/β-sodium glycerophosphate/polydopamine (CS/β-GP/PDA) gel with significantly enhanced adhesion was prepared. Rheological experiments proved that the addition of PDA could significantly reduce the gel temperature of the hydrogel and shorten the in situ gelling time. CCK-8 experiments showed that CS/β-GP/PDA and CS/β-GP/PDA/NPs gel systems both exhibit minimal cytotoxicity. In the porcine esophagus simulation experiment in vitro, after the sol was injected into the wound, the gel could be formed in situ, and the gel adhered to the wound strongly. Through measuring the drug release rate, it was verified that the release rate of the two drugs contained in the CS/β-GP/PDA/NPs hydro- gel was significantly different, suggesting that the phased dosing could be achieved. Conclusion: The designed CS/β-GP/PDA/NPs gel system is suitable for post-ESD wound repair, and could achieve phased dosing, revealing its potential for preventing post-ESD esophageal stricture. [ABSTRACT FROM AUTHOR]

Objective To analyze the causes of death and years of life lost among patients with severe mental disorders in Jining city, in order to provide references for improving the management level of the patients. Methods A total of 3 638 patients with severe mental disorders who were recorded in the National Information System for Severe Mental Disorders in Jining and died between January 1, 2014 and December 31, 2020 were included in the study. The general information and the status of mortality were extracted via checking management files. Thereafter, the causes of death of patients with different characteristics were discussed, and the years of life lost due to severe mental disorders was analyzed through calculating potential years of life lost （PYLL), average years of life lost （AYLL） and potential years of life lost rate （PYLLR). Results The majority of patients who died from severe mental disorders were those with schizophrenia, accounting for 77. 68% （2 826/3 638). The most common cause of death among patients with severe mental disorders was physical illness with 1 869 cases （51. 37%）. Among the selected subjects, patients with mental retardation and comorbid mental disorders had the youngest age of disease onset ［（22. 49±20. 14） years], the youngest age at death ［（51. 72±18. 32） years］ and the longest duration of disease ［（29. 26±19. 35） years］. The PYLL, AYLL and PYLLR of patients with severe mental disorders in Jining were 68 941. 06 person-years, 18. 95 years and 382. 36‰, respectively. The mental retardation and comorbid mental disorders had the highest AYLL at 27. 21 years, and epilepsy-induced mental disorder had the highest PYLLR at 892. 73‰. Conclusion Comorbid physical illness is the main causes of death in patients with severe mental disorders in Jining city, and epilepsy-induced mental disorder have occupied the first place in terms of the years of life lost. [ABSTRACT FROM AUTHOR]

Objective: To analyze the clinical characteristics of patients with chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis (PF), and the detection significance of serum transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF) and tumor necrosis factor-α (TNF-α). A total of 80 patients with COPD admitted to the hospital from January 2020 to January 2022 were selected as the study subjects. They were divided into COPD with PF group (n=35) and COPD group (n=45) according to the presence or absence of PF. The clinical manifestations, pulmonary function, blood gas analysis, chest X-ray film, chest CT and HRCT results were analyzed. Serum TGF-β1, CTGF and TNF-α levels were detected. ROC curve was used to analyze the predictive performance of serum TGF-β1, CTGF and TNF-α levels for PF in COPD. The clinical symptoms of patients with COPD and PF were between the two diseases. Pulmonary function test showed mixed ventilation dysfunction, while DIco diffusing capacity showed moderate to severe reduction. Blood gas analysis showed obvious hypoxemia. Chest X-ray film and chest CT showed characteristics of the two diseases. Serum TGF-β1, CTGF and TNF-α levels in the COPD with PF group were significantly higher than those in the COPD group (P<0.05). ROC curve analysis showed that the sensitivity and AUC of serum TGF-β1, CTGF and TNF-α levels in combined diagnosis of COPD complicated with PF were 96.00% and 0.894, higher than those of single diagnosis (P<0.05). Patients with COPD and PF have clinical manifestations of both COPD and PF, which makes the manifestation of each disease atypical, forming unique clinical manifestation, thereby providing a basis for the diagnosis and treatment of the disease. Serum TGF-β1, CTGF and TNF-α levels are high in patients with COPD and PF, suggesting that these indicators can be used to predict COPD complicated with PF. [ABSTRACT FROM AUTHOR]

Objective To systematically evaluate the reliability of massage treatment for patients with knee osteoarthritis using evidence-based medicine method.Methods The relevant literatures were retrieved from PubMed,Cochrane Library,Emabase,ScienceNet.cn,World Wide Web,Wanfang,VIP,CNKI and China Biomedical literature database since the establishment of the database.RevMan 5.3 software was used for data analysis.The experimental group was treated with massage combined with relevant Chinese medicine or western medicine,while the control group was only treated with western medicine.The Cochrane Library tool for assessing risk of bias for randomized controlled trials was used.Two reviewers independently selected research,extracted data and assessed bias risk.And quantitative data was analyzed by meta-analysis.Results A total of 16 studies with 1 670 patients were included.The meta-analysis results showed that the experimental group was significantly better than the control group in overall effective rate [odds ratio(OR)=4.53,95 %confidence interval(CI) 3.06-6.69,P <0.000 01],visual analogue scale score [mean difference(MD)=-2.72,95 %CI-4.19--1.25,P<0.000 01],WOMAC score [MD=-14.21,95 %CI-14.86--13.56,P<0.000 01],HSS score [MD=6.32,95 % CI 4.58-8.06,P <0.000 01] and other aspects.Conclusion The clinical effect of massage combined with relevant Chinese medicine or combined with western medicine in the treatment of knee osteoarthritis is significantly more than that of western medicine alone. [ABSTRACT FROM AUTHOR]

Objective: To study the correlation between leukocyte count (WBC), C-reactive protein (CRP), uric acid (UA) and glucose and lipid metabolism, insulin resistance and liver fibrosis indexes in military pilots with nonalcoholic fatty liver disease (NAFLD). Methods: 100 cases of military pilots with NAFLD who were admitted to Navy Qingdao Special Service Convalescent Center from July 2018 to December 2021 were selected as the NAFLD group, and 90 healthy people in the same period were selected as the control group. WBC, CRP, UA, glucose and lipid metabolism, insulin resistance and liver fibrosis related indexes were detected and compared between the two groups. Pearson correlation coefficient was used to analyze the correlation between WBC, CRP, UA and glucose and lipid metabolism, insulin resistance and liver fibrosis indexes. Results: The levels of WBC, CRP and UA in NAFLD group were significantly higher than those in control group (P<0.05). The levels of fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), fasting insulin (FINS) and homeostasis model insulin resistance index (HOMA-IR) in NAFLD group were higher than those in the control group, and the level of high density lipoprotein cholesterol (HDL-C) was lower than that in the control group (P<0.05). The levels of type Ⅲ procollagen peptide (PC-Ⅲ), laminin (LN), hyaluronic acid (HA) and type Ⅳ collagen (Col-Ⅳ) in NAFLD group were significantly higher than those in control group (P<0.05). Pearson correlation analysis showed that WBC, CRP and UA were positively correlated with TC, TG, LDL-C, HOMA-IR, PC-Ⅲ, LN, HA and Col-Ⅳ, but negatively correlated with HDL-C (P<0.05). WBC, CRP and UA had no significant correlation with FBG and FINS (P>0.05). Conclusion: WBC, CRP and UA in patients with military pilots with NAFLD increased significantly, which are related to the disorder of glucose and lipid metabolism, insulin resistance and liver fibrosis. [ABSTRACT FROM AUTHOR]

The application efficiency of UAV 3D in the field of antique building reconstruction is focused on. Guilin scenic area of Yanshan garden show park is taken as the research area. UAV tilt photogrammetry is used for aerial photo and RTK for data acquisition of image registration and check data. The real 3D model with real texture is obtained by ContextCapture performing, aerial triangulation, 3D reconstruction and other operations on the original data of oblique photogrammetry. After verifying the model plane and elevation accuracy, DP-Modeler is used to reasonably repair the scene and measure the architectural proportions of the antique buildings. The results are applied to SketchUp and 3ds Max for detailed modeling of typical archaized buildings in the scenic area of Dayan Pavilion, and the detailed modeling of the monolized buildings is obtained respectively. In analysis the detailed structure reconstruction and individual fine reconstruction, it is found that the visualization this method is better than that of traditional tilting 3D reconstruction,and SketchUp's modeling efficiency is better than 3ds Max in modeling the archaized buildings. With the same visualizations, SketchUp is 2. 09 times larger than 3ds Max project files, with 9. 66% more pixels, and 72. 97% more model TIN. It provides a new idea for the visualization of archaized architecture. [ABSTRACT FROM AUTHOR]

To explore the characteristics of Pb2+ adsorption by wetland vegetation type peat in the Tianbaoyan National Nature Reserve, Fujian, field investigations of peat of Pllostachys heteroclada, Palhinhaea cernua, Juncus effuses, and Sphagnum magellanicum were performed. Based on basic indicators of the peat moss of the four wetland vegetation types, the effects of peat input, time, pH value, Pb2+ concentration, and other factors on the Pb2+ adsorption performance of the four wetland vegetation types were evaluated, and an isothermal adsorption model was developed. The method assessed the Pb2+ adsorption characteristics of the four types of peal. The results showed that the absorption capacities of the four types of wetland vegetation peal decreased with increase of input and that the adsorption rates increased with increase of adsorption time. The adsorption capacities for Pb2+ of the four types of peat reached their maximum at 30 min. Furthermore, the adsorption capacities increased with increase of Pb2+ concentration. However, the change trends of adsorption rate were inconsistent. The adsorption capacities of S. magellanicum and J. effuses were 4.59 and 4.51 mg· L-1, respectively, at pH value= 6, and the maximum adsorption capacities of Pllostachys heteroclada and Palhinhaea cernzw were 4. 72 and 4.97 mg· L-1, respectively, at pH value= 8. The adsorption characteristics of the four peats were more consistent with the Langmuir model, and the fitting effect of Palhinhaea cernua peat was best. [ABSTRACT FROM AUTHOR]

BACKGROUND: Studies have found that drugs that block the secretion of inflammatory transmitters in the synovium have good application prospects in the treatment of acute gouty arthritis. In recent years, great achievements have been made in the treatment of acute gouty arthritis with traditional Chinese medicine. OBJECTIVE: To study the effect of alcohol extract of Gardneria angustifolia on serum inflammatory factors and synovial cell function in patients with acute gouty arthritis. METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into six groups: blank control group, model control group, colchicine treatment group, Gardneria angustifolia alcohol extract high, medium and low dose treatment groups, withy 10 rats in each group. In addition to the blank control group, the other groups were injected with sodium urate solution into the articular cavity to prepare acute gouty arthritis models, and then treated with colchicine, high, medium and low doses of Gardneria angustifolia alcohol extract. The swelling degree of the joint was observed at 1-7 days after modeling. At 7 days after modeling, the levels of interleukin-1β, tumor necrosis factor-α, interleukin-17 in the serum and synovium were detected by ELISA; the pathological changes of the synovium were observed by hematoxylin-eosin staining, and the expression of nuclear factor- κB, ERK1/2 and JNK protein in the synovium was detected by western blot. RESULTS AND CONCLUSION: In the model control group, the joint swelling, the level of interleukin-1β, tumor necrosis factor-α, and interleukin-17 in the serum and synovium were increased, the synovium had obvious inflammatory cell infiltration and synovium cell proliferation, the expression of nuclear factor-κB, ERK1/2 and p-JNK protein was significantly increased. After the treatment with colchicine and Gardneria angustifolia alcohol extracts, the above indexes were significantly improved, and there was no significant difference in the high dose Gardneria angustifolia alcohol extract group and colchicine group. Moreover, Gardneria angustifolia alcohol extracts showed a dose-dependent effect. The alcohol extract of Gardneria angustifolia can significantly improve the symptoms of acute gouty arthritis in rats, down-regulate the expression of inflammatory factors, and improve the function of synovial cells, which may be related to the down-regulation of nuclear factor-κB, ERK1/2 and p-JNK protein expression. [ABSTRACT FROM AUTHOR]

In this study, we propose a design of the whole process supervision system of construction lands sustained by the business flow of construction lands. The data governance model is constructed from the whole process perspective based on the construction project “batch, supply, use, complement, check.” Data association is reconstructed, and the whole process data achievements are obtained. By constructing an index model of supervision, monitoring, and evaluation, data concatenation, query statistics, and dynamic monitoring of the entire process of construction land are realized. [ABSTRACT FROM AUTHOR]

Tropics are more fragile to climate change, especially in tropical island. It's has not been investigated the change of agricultural heat and precipitation resources in future in tropical island like Hainan island, China. Because there are a lot of space biases between the raw CMIP5 data set and the observed values in Hainan island. Daily maximum temperature, minimum temperature and precipitation were obtained from the ground weather stations and the GCMs include FGOALS-g2, GFDL-ESM2G, HadGEM2-ES, MPI-ESM-MR and MRI-CGCM3 in Hainan island and its nearby waters. The observations and the raw GCMs outputs for the historical (1970-1999), RCP2.6, RCP4.5 and RCP8.5 (2020-2099) scenarios were processed and interpolated to a spatial resolution of 0.5°×0.5° as grid cells using the bilinear method. We used both systematic residuals revision methods (corrected value method or ratio method) and multi-mode ensemble averaging methods include the Bayesian model averaging (BMA) method and the equal weight averaging (EW) method in each grid cells to reduce the spatial uncertainty of the raw GCMs in the training and verification period. And then, we used the revised GCMs outputs and the agro-climatic index computing software to analysis the change of agricultural heat and precipitation resources under the scenarios of RCP2.6, RCP4.5 and RCP8.5 in both short-term (2020-2059) and long-term (2060-2099). These sources include annual mean temperature, mean temperature in January, ≥10°C and ≥20°C integrated temperature, annual precipitation, precipitation in January and precipitation in ≥20°C integrated temperature period. The results showed that the correct coefficients of the raw GCMs outputs from both systematic residuals revision and the BMA method all have large spatial differences among the grid cells. The raw GCMs outputs underestimate the daily maximum temperature about 3.55°C, overestimate the daily minimum temperature about 1.19°C and underestimate the daily precipitation which only 54.35% of the observations. It can effectively reduce the spatial uncertainty of the raw GCMs outputs by the above revision methods. The revised results of the BMA and the EW are similar and both are better than a single GCM for simulate historical climate variables. After comprehensive revision of the BMA in each grid cells, the correlation coefficients of maximum temperature, minimum temperature and precipitation are increased about 0.10, 0.07 and 0.06 respectively, and the root mean square error are reduced about 2.38°C, 1.01°C and 1.01mm respectively, in the verification period. There are decreased about 3.25°C, 1.13°C and 25.67mm compared with the average biases of a single GCM and closer to the observed value. In the future, the agricultural heat resources will generally show a gradual increase from the central mountains to the coast in spatial. The high temperature will distribute mainly range from the southern to the western coastal areas. The annual mean temperature will increase evenly in the whole island. The increasing amplitude of mean temperature in January, ≥10°C and ≥20°C integrated temperature has different patterns that will decrease from the eastern to the western, from the northern to the southern, and from the central mountains to the coast, respectively. It will increase significantly with the fastest climate trend rate under the RCP8.5, or increase first in short-term and then level off in long-term under the RCP4.5, or relatively flat without increase significantly under the RCP2.6. The precipitation resources are transforming into a pattern of gradually decreasing from the eastern to the western and with no significant trend in temporal. The precipitation variability will increase in the southern and the northern coastal areas, while decrease in the western and the central areas. With climate warming and the changes of precipitation pattern in future, the expansion of suitable crop cultivation areas will face huge challenges to agricultural production. It is necessary to arrange in advance to seek advantages and avoid disadvantages. [ABSTRACT FROM AUTHOR]

In corner detection, the ability of two-dimensional Gabor feature to distinguish corners is not verified, and the description of pixel neighborhood features has defects.As the result, the robustness of corner detection is poor and the positioning accuracy is low. In order to solve the above problems, the directional derivative of the real part of the two-dimensional Gabor filter was used to extract the intensity variation difference of each corner model,with the multi-directional structure tensor and multi-scale screening mechanism, a new corner detection algorithm was proposed. Firstly, the input image was smoothed by the real part of the two-dimensional Gabor filter, and the gray variations in all directions were extracted. Secondly, the multi-directional structure tensor product was constructed by synthesizing the gray-scale variation information of the target pixel in each direction, and the corresponding corner measure was generated. Finally, the non-maximum suppression was used for preliminary screening, and then the target corners were obtained by multi-scale filtering. The performance of the proposed method was compared with seven classical detection algorithms under the conditions of image affine transformation and Gaussian noise interference. The proposed method has better detection robustness, and the positioning accuracy is also verified by matching experiment based on ground truth. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of open and closed reduction and internal fixation with hollow nails on the quality of fracture reduction and hip joint function in patients with displaced femoral neck fractures. Methods: This study is a retrospective study. The clinical data of 98 patients with displaced femoral neck fractures were selected. According to different surgical methods, the patients were divided into group A (n=50, open reduction) and group B (n=48, Closed reduction), compare the good and good rate, fracture reduction quality, hip joint function, perioperative index, postoperative complication rate and secondary operation rate between the two groups. Results: The excellent and good rate of patients in group A was 78.00% (39/50) at 6 months after surgery, which was higher than 58.33% (28/48) in group B (P<0.05). There was no difference between the two groups in postoperative weight-bearing time and intraoperative fluoroscopy time (P>0.05); the operation time and hospital stay in group B were shorter than those in group A, and the amount of intraoperative blood loss was less than that in group A (P<0.05). The Harris scores of hip joint function at 1 month, 3 months, and 6 months after operation were higher than those before operation in the two groups, and group A was higher than group B (P<0.05). There was no difference in the incidence of secondary operations and complications between the two groups (P>0.05). The number of type I and type II cases in group A were more than that in group B, and the number of type III cases was less than that in group B (P<0.05). Conclusion: Compared with closed reduction cannulated screw internal fixation, open reduction cannulated screw internal fixation is more damaging, but its postoperative fracture reduction quality and hip joint function improvement effect is better, and it does not increase the incidence of complications and secondary complications. The incidence of surgery. [ABSTRACT FROM AUTHOR]

In order to chffify the effects of difforcnt human activities on hydrological regime, this paper analyzed the hydrological regime similarity in different anthropogenic influence phases by using [0·11m11ic Time Warping ( DTW), based on the daily runoff from 1954 to 2016 in W'enjiachuan Hydrologic Station. The results show that: a) both soil and water conservation and coal mining have mmlc a considerable reduction of the river runoff. The effect of water stored by coal mining goaf is larger than that by soil m1d water conservation ; b) the DT\V distance during weak m1thropogenic influence ( stage I ), soil and water conservation ( stage II )

Based on three technology needs assessment (TNA) reports of the United Nations, this study analyzed the key fields and technology needs for climate change adaptation, and specified the key fields and technology needs in different regions. The results showed that: 1 agriculture and fisheries were the optimal key field for all countries in the world for climate change adaptation, followed by coastal zone management, water resources and health care. The number of priority technology needs for climate change adaptation was consistent with that of key fields, with the greatest technology needs in agriculture, forestry and fishery, followed by coastal zone management, water resources and health care. Priority technology needs in the field of agriculture, forestry and fisheries mainly focused on crop management, efficient irrigation, land management and improved animal husbandry, with the greatest need in the development and use of drought-tolerant/heat-tolerant crop varieties. 2 different regions showed different emphasis on the key fields. While agriculture and forestry were the most needed key fields for all regions, African countries regarded water resources as priority; Asia Pacific, Latin American and Caribbean regions focused on water resources and health care; the least developed countries emphasized on agriculture modernization and forestry; small island developing states considered the technologies for coping with sea level rise and food security as main demand; and European and Commonwealth of Independent States showed an average need for the key fields. 3 All countries have identified barriers for priority technology development and transfer, concerning economy and finance, policy, law and regulatory, institution and organization, as well as technical barriers. Different regions showed the same lack or inadequate access to financial resources and information. Latin American and Caribbean regions reported barriers relating to traditions and habits, and Eastern European countries identified barriers relating to the high cost of production and financial viability of priority technologies. Future key fields and technology developments will mainly focus on the cultivation technology of new agriculture, water resources conservation and seawater desalination technology, etc. These key fields and technology developments can provide reference for China to strengthen international cooperation on climate change. [ABSTRACT FROM AUTHOR]

BACKGROUND: The diagnosis and treatment of the terrible triad of the elbow have achieved a breakthrough. OBJECTIVE: To introduce the definition, basic anatomy, stable structure, injury mechanism and classification of terrible triad or the elbow, and to discuss the treatment plan deeply. METHODS: The first author searched PubMed, CNKI and Wanfang databases for the articles published before September 2019. The keywords were "terrible triad of the elbow, elbow joint, fracture dislocation" in English and Chinese, respectively. A large number or articles concening the terrible triad of the elbow in recent years were reviewed, and the treatment strategy was summarized. RESULTS AND CONCLUSION: For the terrible triad or the elbow, the operator must carefully check the patient before operation, carefully analyze the imaging examination results and three-dimensional printed model, and sea.irately judge the bone structure of the elbow and degree of ligament injury. During the operation, the stability of the radial head, the fracture of the coronoid process and the injury of the lateral ligament complex are usually reconstructed through a single lateral approach or a combination of internal and external approaches. Then, the stability of elbow joint is tested to determine whether it is necessary to repair the medial ligament complex or use the hinged external fixator to restore the stability of elbow joint. Early active rehabilitation is needed. The above systematic treatment can reduce postoperative complications, accelerate fracture healing, and improve elbow joint function and patients' quality of life. [ABSTRACT FROM AUTHOR]

The effects of recycled rubber resin on the three indices of asphalt were investigated, and the influence of recycled rubber resin on the stabilities of asphalt segregation and high temperature was also discussed. A new recycled rubber resin composite modifier was developed, and the adaptability of three kinds of typical asphalt to the recycled rubber resin composite modifier was evaluated. The results show that with the increasing of recycled rubber resin content, the asphalt penetration is decreased, while the ductility and the softening point are increased gradually. With the increasing of recycled rubber powder dosage, asphalt penetration, ductility and softening point are all increased with latter decreasing, while the segregation degree between recycled rubber resin and asphalt is decreased with latter increasing. However, the dynamic stability of asphalt mixture is increased gradually. The asphalt ductility is obviously improved by 2~# styrene butadiene rubber, and the newly developed recycled rubber resin composite modifier has good adaptability to the matrix asphalt. [ABSTRACT FROM AUTHOR]

BACKGROUND: Construction of tissue engineering vascular structures is a key to the regeneration of complex tissues and organs. The use of three-dimensional printing technology to construct vascular structures has become a hotspot. OBJECTIVE: A highly connected and perfusable vascular network was rapidly deposited by a three-dimensional bioprinter with a coaxial nozzle using alginate/silk fibroin bioink. METHODS: A mixed solution containing 5% sodium alginate and 5% silk fibroin as the bio-ink, and a mixed solution containing 5% calcium chloride and 13% F127 as the crosslinker, alginate/silk fibroin gel was printed using a bio-printer and subjected to optical coherence tomography and scanning electron microscopy. The solution containing 5% sodium alginate and 5% silk fibroin mixed with the suspension of human liver cancer cells (C3A) as the bio-ink, and a mixed solution containing 5% calcium chloride and 13% F127 as the crosslinker, the cell-containing sodium alginate/silk fibroin gel was printed on a bio-printer. Then, the printed scaffold was placed in the medium for 24 hours, and then stained by Calcein-AM and observed under a fluorescence microscope. RESULTS AND CONCLUSION: (1) Optical coherence tomography: a multi-layer composite hollow tube network formed the structure of the scaffold. The gel filament had a complete hollow structure, and the channels were interconnected. The structure was similar to the hollow passage of the blood vessel, which was beneficial to the transportation of nutrients and metabolic waste. (2) Scanning electron microscope: the hollow pipe structure with the diameter of about 400 μm arranged in parallel, and the pipe boundaries were clear. The microporous structure caused by the removal of F127 was formed between the boundaries. (3) Fluorescence microscope: the cells were evenly dispersed in the material on both sides of the channel, and the cells grew well in the scaffold with the survival rate of above 95%. (4) These results suggest that three-dimensional bioprinting technology based on coaxial nozzles and sodium alginate/silk fibroin bio-ink can be used to construct vascularized functional tissues in the future. [ABSTRACT FROM AUTHOR]

Objective To observe the interventional effect of Huoxue Jiedu Fang (Bloodactivating Toxin-removing Formula) on skin lesions in psoriasiform mouse model induced by imiquimod, and clarify the importance of detoxification Chinese medicinals in treatment of psoriasis. Methods Male BALB/c mice (n = 55.) were randomly divided into normal control group, model group, Huoxue Jiedu Fang group (HJF group), Huoxue Fang (Blood-activating Formula) group (H F group) and methotrexate group (MTX group, each n=11). The psoriasiform mouse model w as induced by external coating imiquimod. IVTTXgroup wa s orally given MTX(1 mg/kg), HJF group, HJF (35.1 g/kg), HF group, HF (26.9 g/kg), while normal control group and model group, equivalent pure water. All mice were executed on the 7 th d for collecting skin tissue samples. The scores of psoriasis area and severity index (PASI) were observed. The histomorphological changes and epidermal layer thickness of skin lesion tissue were observed by using light microscope after HE staining. The expressions of proliferating cell nuclear antigen (PCNA), marker on T-cell (CD3) and positive microvessel marker (CD31), and microvessel density (M V D) were detected by using immunohistochemistry technique. The m RNA expressions of interleukin-17 (.IL-17.) and interleukin-23 (IL-23) were detected by using real-time PCR. Results The results of H E staining showed that epidermal proliferation, para keratosis cells and epidermal thickness decreased in HJF group compared with model group (P<0.001). The positive expressions of P CNAand CD3 were significantly lower in HJF group than those in model group (P<0.001:). MVD was significantly lower in HJF group than that in model group (P<0.05). The m RNA expressions of inflammatory factors (IL 17, I L-23) were significantly lower in HJF group than those in HF group (P<0.05). Conclusion Huoxue Jiedu Fang can inhibit blood vessel proliferation, regulate IL-23/IL-17 axis, reduce expressions of Th 17 related factors and relive proliferation of epidermal keratinocytes and infiltration of dermis immunocytes, and thereby relieve the psoriasiform lesions induced by imiquimod in mice. [ABSTRACT FROM AUTHOR]

ObjectiveTo establish a microRNA-mRNA differential expression network for alcoholic hepatitis (AH), and to investigate new targets for the diagnosis and treatment of AH. MethodsDifferentially expressed microRNAs and mRNAs between AH patients and normal controls were screened out. Related software including TargetScan, DIANA, MIRDB, PICTAR, and miRWalk 2.0 was used to search for the target genes of differentially expressed microRNA, and a key microRNA-mRNA network was established using the differentially expressed mRNAs that changed in an opposite way to microRNA. The Database for Annotation, Visualization and Integrated Discovery was used for the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) analyses of target genes. The GCBI online software (www.gcbi.com.cn) was used for enrichment analysis of target genes and core network establishment. The GeneMANIA database in Cytoscape software (genemania.org) was used to perform a protein-protein interaction analysis of key target genes. The above three methods were compared in terms of the search for key pathways involved in the development of AH. ResultsA key microRNA-mRNA network was established with 5 differentially expressed microRNAs including hsa-mir-21-5p, hsa-mir-148a-3p, and hsa-mir-30e-5p and 51 target genes including collagen type IV alpha 1 chain (COL4A1), thrombospondin-2 (THBS2), and integrin alpha 6 (IGTA6). A protein-protein interaction network of key target genes was established. The GO analysis and various pathway analyses showed that the PI3K-Akt pathway and local adhesion were closely associated with AH. ConclusionDuring the development of AH, there are complex interactions between the related proteins of key target genes. COL4A1 and THBS2 may promote the development of AH by activating ITGA6 to regulate the PI3K-Akt pathway and the process of local adhesion. The establishment of the microRNA-mRNA network reveals the key links in the development of AH and highlights the focus of research. The discovery of the genes associated with the PI3K-Akt pathway in AH is expected to provide new targets for the diagnosis and treatment of AH. [ABSTRACT FROM AUTHOR]

Combined with the development of regional industries, based on the concept of CDIO, teaching reform of professional experiment courses was carried out by integration of product-oriented teaching content and design of teaching process with CDIO in biological engineering, pharmaceutical engineering, polymer materials and engineering, chemical engineering and technology. This aimed to cultivate students' professional knowledge, engineering practice ability and interpersonal team ability. The practice showed that experiment teaching quality was improved significantly, the experimental teaching system was optimized and cultivation mode of high quality applied talents was promoted. These supported to build a high level application university with regional characteristics. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical significance of serum vitamin D levels change in patients with breast cancer(BC)before and after radiotherapy and chemotherapy. Methods A total of 100 BC patients and 100 healthy controls were enrolled in the First Affiliated Hospital of Xi'an Jiaotong University from June 2016 to June 2017. All the patients received adjuvant chemotherapy and radiotherapy after operation. Serum 25-hydroxyvitamin D3(25-OHD) levels were measured using the DRG ELISA kit. Results There was no significant difference in age, BMI, times of deliveries, hemoglobin level, total lymphocyte count and platelet count between the cases and the controls (P>0.05). The incidence of vitamin deficiency was 100% in the case group and 85% in the control group. The level of serum vitamin D was (16.63 ± 1.83) ng/ml in the case group before radiotherapy and chemotherapy, and (25.56 ± 6.64) ng/mL in the control group. The level of vitamin D before radiotherapy and chemotherapy in the case group was significantly lower than that of the control group (P<0.001). The mean level of vitamin D was (11.35 ± 2.87) ng/mL after radiotherapy and was significantly lower than that before radiotherapy and chemotherapy(P<0.001). Two different chemotherapy regimens were used in this study, FEC/T and CMF regimens. Before radiotherapy and chemotherapy, there was no significant difference in vitamin D levels between the two groups (P>0.05). After radiotherapy and chemotherapy, vitamin D levels in both groups were significantly decreased (P<0.001), but there was no significant difference between the two groups in vitamin D levels (P>0.05). Multiple regression analysis showed that age, BMI and number of deliveries were significantly correlated with changes of vitamin D levels before and after radiotherapy and chemotherapy (P<0.05), and correlations were as follows: age, BMI and times of deliveries. Conclusion Vitamin D deficiency may be associated with breast cancer. Patients' age, BMI and times of deliveries were significantly correlated with vitamin D levels before and after radiotherapy and chemotherapy. [ABSTRACT FROM AUTHOR]

As the carrier of resources and ecological environment, territorial space is the basis for promoting green development and lifestyle. The scientific distribution of production space, living space, and ecological space plays an important role in the modernization of the national governance system and the governance capacity. Based on the construction of a basic information platform of provincial land space, this paper discusses the construction of a large data system of land resource space, analyzing the application of data resources in related fields. [ABSTRACT FROM AUTHOR]

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUC-MSCs) have low immunogenicity and it is unclear whether insulin producing cells (IPCs) that differentiate from hUC-MSCs have low immunogenicity. OBJECTIVE: To investigate the immunogenicity of IPCs differentiating from hUC-MSCs in vitro and after IPCs transplantation into the host. METHODS: (1) The hUC-MSCs were induced to differentiate into IPCs according to the modified scheme. Flow cytometry assay was used to detect the immunophenotype and apoptotic rate of IPCs in a cytotoxicity test. (2) Cell counting kit-8 was used to detect the proliferative capacity of human peripheral blood mononuclear cells in the one-way mixed lymphocyte assay. (3) The IPCs were then transplanted into the abdominal cavity and left renal capsule of mice, and then the infiltration of immune cells was detected by flow cytometry and immunohistochemistry. RESULTS AND CONCLUSION: The IPCs highly expressed HLA-ABC and lowly expressed HLA-DR, CD40 and CD80. The apoptosis rate of IPCs increased with the increase of pre-sensitized splenocytes in the cytotoxicity test. In the one-way mixed lymphocyte assay, IPCs inhibited the proliferation of human peripheral blood mononuclear cells when the target ratio was 10:1 and 50:1. After IPCs transplantation, the number of lymphocytes was increased in the transplanted site. In summary, our results show that IPCs that differentiate from hUC-MSCs maintain low immunogenicity in vitro, but have some immunogenicity after transplantation into the host due to microenvironment changes. [ABSTRACT FROM AUTHOR]

The construction experiences of Provincial Experimental Teaching Demonstration Center of Biotechnology and Chemical Engineering are summarized. To cultivate the practice and innovation ability of students, attach great importance to the transformation of idea, content and model about experimental teaching, an innovative experimental teaching system and practice teaching mode involving university-enterprise cooperation art* built, the establishment has been proved to meet the social demand for talents and improve the educational level and quality of talent cultivation in applied independent college. [ABSTRACT FROM AUTHOR]

Objective To identify the related factors of osteoporosis in breast cancer women 1-year after the surgery. Methods Forty-one breast cancer patients 1-year after surgery aged from 53 to 75 years old with osteoporosis were selected. Fifty-six breast cancer patients 1-year after surgery aged from 46 to 64 years old with normal bone mineral density (BMD) were selected in the control group. BMD of the lumbar spine and the left femoral neck was detected using dual-energy X-ray absorptiometry (GE, USA). The correlation analysis among age, BMI, reproductive history, postmenopausal time, ER, PR, HER-2, and BMD was performed. Results There was significant difference in age, BMI, and postmenopausal time between OP group and NOP group (P<0.05). However, there was no difference in ER, PR, HER-2, and reproductive history between the two groups (P>0.05). Correlation analysis indicated that BMD was positively correlated with BMI, but was negatively correlated with age and postmenopausal time. Conclusion The prevalence of osteoporosis in women with breast cancer is high. The main influential factors are age, BMI, and postmenopausal time. [ABSTRACT FROM AUTHOR]

Objectives: The straight baseline, which has the advantages of easy management, less correlation with the change of low tide line, and being conductive to the parties to obtain a lager jurisdiction area, is widely used in the baseline points selection and baselines determination of territorial sea in coastal countries. However, as coastal countries pay more attention to maritime rights and interests, the selection of baseline points in the current straight line system has become an urgent problem to be solved. Methods: First, based on the determination of ideal baseline points by convex hull construction algorithm, and combined with the limitation of baseline length in the United Nations Convention on the Law of the Sea, the baseline graph construction and prioritization theory based on alternative baseline points is analyzed. Then, by erecting the intervisibility judging principle between baseline points, a fast judgment and optimal selection model of alternative baseline points under the principle of maximum internal water area is established, and an alternative baseline point order determination steps for optimal baseline graph is designed. Finally, we propose an optimal selection algorithm of territorial sea baseline points with the limitation of baseline length threshold, which realizes the rapid selection of alternative baseline points under the limitation of baseline length threshold and internal water area maximization. Results: The results show that the proposed algorithm can minimize the polygon area of the baseline graph (maximum the area of internal water) constructed by the optimal base point under the condition of baseline length limitation, and has a high algorithm efficiency. Conclusions: Our proposed The algorithm can provide technical support for the selection of the baseline point of territorial sea of coastal countries, especially the archipelago countries and the determination of the straight baseline. [ABSTRACT FROM AUTHOR]

In recent years, Ebola virus (EBOV) has caused widespread public concern because of its severe outbreaks in Africa. The quantity of domestic and foreign patent application in China, patent year distribution, technology development relates to the detection and prevention of Ebola virus was statistically analyzed, together with the summary of the detection, immunization, therapy technology in patents, and the brief comparison of the patents information of focus drugs and vaccine (jk-05, ZMapp, VSV-EBOV). It was found that the foreign applicants dominates in the amount of patent application and technology diversity in patents, compared to the domestic applicants, providing reference for domestic researchers. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of mutated isocitrate dehydrogenase 1 (IDHl) on TAZ (transcriptional coactivator with PDZ-binding motif protein) expression in human astrocytoma and to explore the relevant mechanisms. Methods Glioblastoma (GBM) U87MG cells transfected with mutated IDH1-132H and wild type IDH1-132R (WT) were used to detected the TAZ protein expression by Western blot; The expression of TAZ protein and its cytoplasmic binding protein 14-3-3e were investigated in a cohort of GBM cases, which including 14 IDH1 wild type cases and 7 IDH1 mutated cases. In vitro, qRT-PCR was used to detect TAZ mRNA expression; Western blot was used to detect the levels of LATS1, phosphorylated TAZ and 14-3-3e protein. Results We found that the expression of TAZ protein was decreased in IDH1 mutated cells compared with those of IDH1 wild type cells. This result was further verified in vivo by using immunohistochemistry to detect the expression of TAZ in human GBM samples with or without IDH1 mutation. Immunohistochemistry also showed that TAZ cytoplasmic binding protein 14-3-3e was increased; qRT-PCR showed that TAZ mRNA level was decreased in IDH1 mutated cells; and subsequent Western blot showed the levels of LATS1, TAZ phosphorylation and the 14-3-3e were increased in IDH1 mutant cells. Conclusions IDH1 mutation in gliomacould reduce TAZ mRNA and its protein expression, increase the phosphorylation level of TAZ protein by activating Hippo signaling pathway, thereby furtherinhibit TAZ expression. [ABSTRACT FROM AUTHOR]

Objective: To investigate and analyze the reference interval for the detection of blood coagulation indexes and partial biochemical indexes in infants. Methods: 2683 infants who were admitted into the ten top-three hospitals between January 2012 and June 2014 were treated as the study object, including 1700 cases of terminfants and 983 cases of premature infants. 1000 staff who underwent health examination in the ten hospitals in the same period were selected as the control group. Blood coagulation indexes and 12 biochemical indexes were measured by automatic blood coagulation analyzer and automatic biochemical analyzer and the reference interval for related indexes was established. Result: The blood coagulation indexes such as PT, TT and APTT of the term infants were longer than that of the control group and Fib was decreased with statistical significance (P=0.000). PT, TT and APTT of premature infants was longer than that of terminfants and Fib was decreased (P<0.05). The Glc level in term infants was decreased compared with control group (P= 0.000) and it was lower in premature infants than that in term infants (P=0.000). The serum calcium level in term infants was lower and the contents of serum TC, Cr, BUN and ALT were also lower than those in the control group (P=0.000), and those in premature infants were relatively lower (P<0.05). LDH, CK enzymatic activity and K+ level in term infants were higher than those in the control group(P<0. 05) and those in premature infants were relatively higher (P<0.05); the serum Alb, TP and Na+ levels in term infants were lower than those in the control group (P<0.05) and those in premature infants were lower (P<0.05). Conclusions: Blood coagulation expression in infants is different from that in adults. The changes of reference intervals of blood coagulation and partial biochemical indexes are related to age. It is necessary to establish reference intervals of blood coagulation and partial biochemical indexes related to age for infants. [ABSTRACT FROM AUTHOR]

In order to explore multifunctional microorganism resources to achieve the effective utilization of agricultural waste, some efficient phosphate-solubilizing bacteria were isolated from the compost of mushroom chaff and chicken manure, and their phosphate-solubilizing abilities were subsequently optimized. Efficient phosphorus-solubilizing bacteria were screened from the compost by inorganic phosphorus selective medium. The selected strain was identified through the combination of morphological observation, physiological and biochemical test and molecular biology identification. Single factor and response surface experiments were used to optimize the inorganic phosphorus-solubilizing conditions of this strain. In addition, the compost potential of this strain was evaluated by determining the ability of releasing organophosphorus and potassium, and indole acetic acid production. An efficient phosphate-solubilizing strain Pb1 was isolated and identified as belonging to Bacillus smithii strain. The highest phosphorus-solubilizing capacity of strain B. smithii Pb1 was achieved when the culture condition was kept as: glucose 15.0 g /L, ammonium sulfate 3 g/L, tricalcium phosphate 7.0 g/L, inorganic salt 0.48 g/L, temperature 55.0℃, rotating speed 225 r/min, initial pH 7.0, loaded liquid 60.0 mL/250 mL, and inoculum volume 3.0%（V/V）. Further response surface optimization showed that under the conditions of tricalcium phosphate 6.4 g/L, rotating speed 207 r/min and loaded liquid 65.0 mL/250 mL, the solubilized phosphorus content in the fermentation broth of strain Pb1 cultured for 3 dreached 534.68 mg/L, which was 1.58 times higher than that before optimization. Moreover, B. smithii Pb1 was found to be capable of detoxifying organophosphates, detoxifying potassium and producing indole-3-acetic acid with the content of 238.99 mg/L, 81.06 mg/L and 23.26 mg/L, respectively. B. smithii Pb1 demonstrated the ability of releasing inorganic phosphorus, organophosphates and potassium, and producing indoleacetic acid at high temperature（55℃）, laying the foundation for the development of B. smithii Pb1 as an aerobic composting bacterial agent. [ABSTRACT FROM AUTHOR]

目的 分析合理情绪疗法治疗终末期肾脏病(ESRD)维持性血液透析患者焦虑, 抑郁的临床效 果。方法 选取2019年1-10月于天府总医院血液透析科, 南桐总医院, 永荣总医院, 松藻总医院就诊及住院 的血液透析伴焦虑, 抑郁且服用抗焦虑, 抑郁药物时间达2周以上的 ESRD 患者56例, 将其分为对照组和研究 组, 2组患者均进行基础治疗(维持抗焦虑抑郁药物治疗, 常规护理), 研究组加用合理情绪疗法, 每周进行疗效 评价, 分别在干预前和干预后1, 2, 4周行汉密尔顿焦虑量表(HAMA), 汉密尔顿抑郁量表(HAMD), 生活满意 度量表(LSIB)及终末期肾病维持性血液透析患者依从性量表(ESRQ-AQ)测定, 进行组间比较分析。结果 干 预后, 研究组及对照组 HAMD, HAMA 得分明显降低, LSIB及ESRD-AQ 得分明显增高, 差异均有统计学意义 (P<0.05)。干预4周后, 研究组 HAMD, HAMA 得分显著低于对照组, 而 LSIB及 ESRD-AQ 得分明显高于 对照组, 差异均有统计学意义(P<0.05)。结论 相较于单纯基础治疗, 基础治疗联合合理情绪疗法 ESRD 对 维持性血液透析患者焦虑抑, 郁情绪有更加明显的改善效果, 有利于提高患者生活满意度及治疗依从性。 [ABSTRACT FROM AUTHOR]

Objective To investigate the significance of using placental superb micro-vascular imaging(SMI) in detection of placental microvessel density(MVD) in patients with preeclampsia(PE). Methods Forty-five PE pregnant women were selected as the PE group, and 45 cases of threatened preterm labor pregnant women were selected as the control group. SMI was used to detect MVD in two groups(SMI-MVD) before delivery of placenta. Immediately after placental tissues were delivered, immunohistochemical SP staining was performed to detect MVD(SP-MVD). The birth weight of two groups was recorded. The SMI-SMI, SP-MVD, and birth weight were compared in the two groups. Spearman method was used to analyze the correlation between SMI-MVD and SP-MVD, SMI-MVD and birth weight, and SP-MVD and birth weight in the PE group. Results In the PE and control groups, the placental SMI-MVD of pregnant women were(0.37 ±0.12) and(0.45 ± 0.11) n/cm2, respectively; the SP-MVD were(104.13 ± 10.008) and(116.44 ± 8.013)/HP, respectively; the birth weight was separately(1991.44 ± 504.28) and(2525.56 ± 377.46) g. Significant difference was found in the SMI-MVD, SP-MVD, and birth weight between the two groups(all P < 0.05). Spearman analysis showed that SMI-MVD was positively correlated with SP-MVD in the PE group(r = 0.244, P < 0.05), SMI-MVD was positively correlated with the birth weight(r = 0.350, P < 0.05). SP-MVD was positively correlated with the birth weight(r = 0.454, P< 0.05). Conclusion Using placental SMI to detect MVD in pregnant women with PE can assess placental microcirculation in PE patients and help predict neonatal birth weight. [ABSTRACT FROM AUTHOR]

目的 观察联合肌注地塞米松和盐酸戊乙奎醚对子宫输卵管造影检查不良反应的预防效果。方法接 受子宫输卵管造影检查的患者200例，随机分为A、B、C、D组，每组50例。A组检查前不应用药物，B组在检查前 30 min时肌注地塞米松5 mg,C组检查前30 min肌注盐酸戊乙奎醚2. 5 mg, D组检查前30 min肌注地塞米松5 mg、盐酸戊乙奎醚2.5 mg。记录各组子宫输卵管造影检查图像噪声、射线剂量及图像质量。记录并比较各组不良 反应发生率和不良反应严重程度。结果各组子宫输卵管造影检查图像噪声和射线剂量较小，图像质量优良率较 高，各组差异无统计学意义。A、B、C、D组不良反应总发生率分别为90. 00%、84. 00%、80. 00%、68. 00%。A组不 良反应总发生率高于其余三组（P均 <0.05)。D组不良反应总发生率及血压下降、喉头水肿、呼吸困难、皮疹、恶 心呕吐、皮肤瘙痒发生率均低于其余三组,D组不良反应严重程度较其余三组减轻（P均<0.05)。结论子宫输 卵管造影检查前预防性应用地塞米松联合盐酸戊乙奎醚可有效降低造影检查不良反应发生率、减轻不良反应严重 程度,且不对造影图像噪声、射线剂量及图像质量产生影响。 [ABSTRACT FROM AUTHOR]

For Volterra model identification of a single-input and single-output nonlinear dynamic system, this paper put forward the grey clustering multi-subpopulation adaptive PSO algorithm and defined accuracy influence coefficient to assess quantitatively the degree of influence that the model structure items impact on the accuracy of identification. At first, the method used Volterra series to create the initial model of nonlinear system, and then, it adopted grey clustering multi-subpopulation adaptive PSO algorithm and accuracy influence coefficient to identify the model structure and parameters. The simulation results show that the proposed method in such aspects as identification precision and convergence speed is superior to other Volterra series identification methods based on standard PSO algorithm, GA and QPSO algorithm methods. [ABSTRACT FROM AUTHOR]

随着护理教育模式的改革, 护理本科生在临床实习中的带教工作日益重要。如何提高带教质量, 保证教 学计划如实落实, 已成为临床带教面临的新课题。 [ABSTRACT FROM AUTHOR]