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[Objectives] This study aimed at deciphering the mechanisms of CVC1302 in promoting germinal center(GC) responses. [Methods]Pattern antigen 4-hydroxy-3-nitrophenylacetyl(NP)-ovalbumin(OVA)were mixed with CVC1302 followed by emulsified with ISA206-named as NP-CVC1302. Six-week-old BALB/ c femal mice were immunized with NP-CVC1302-NP or ISA206-and the sera were collected to detect the NP-specific antibody titers and isotype antibody titers. The percentages of follicular helper T cells (TFH )-NP + GC B cells and NP + plasma-blasts in inguinal lymph nodes and the numbers of GC were measured at 14 days post immunization(dpi). Furthermore-the percentage of NP + long-lived plasma cells(LLPC)in bone marrow was also detected at 42 dpi by using flow cytometry. [Results] Through the detection-we found that NP-specific antibody titers were significantly higher in NP-CVC1302 group compared with NP group(P< 0.01)-as well as the NP-specific IgG1 and IgG2a antibody titers. Significantly higher percentages of TFH cells-NP + GC B cells and NP + plasma-blasts in inguinal lymph nodes were induced in NP-CVC1302 group compared with NP group(P<0.01 or P<0.001). Moreover-significantly higher percentage of LLPC was transferred into the bone marrow of mice that received NP-CVC1302 compared with those that received NP(P< 0.01). [Conclusions] Results of the study revealed that CVC1302 improved the differentiation of TFH cells-then TFH cells selected the high-affinity GC B cells to differentiate into plasma-blasts-and finally became LLPC locating in the bone marrow-continuing to secrete antibody. This study provided the blueprint for designing new generations of immunopotentiators. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao is the property of Journal of Nanjing Agricultural University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)