【Objective】In order to investigate active organic carbon components in 0-40 cm soil layer under comprehensive ecological farming pattern, the sensitivity of active organic carbon (AOC) and characteristics of carbon pool management were examined to provide a theoretical basic for carbon sequestration and nutrient management in paddy field under this pattern.【Method】The soils under three farming patterns, i.e., comprehensive ecological farming mode (EIM), rice-vegetable crop rotation mode (RVM), and conventional rice farming mode (CRM) at the Agricultural Ecological Environment Field Station in Zengcheng District, Guangzhou City, were selected to determine total organic carbon (TOC), dissolved organic carbon (DOC), oxidized organic carbon (EOC), and microbial biomass carbon (MBC), and to explore the factors influencing changes in soil active organic carbon components.【Result】In EIM, TOC was 10.55 g/kg, DOC EOC POC and MBC contents were 0.29, 1.38, 2.09 and 0.11 g/kg, respectively, and those were significantly higher than that of RVM and CRM. Soil microbial entropy was the highest in EIM, and increased by 51.11%-85.72% and 46.04%-223.99%, respectively, compared with RVM and CRM. The most sensitive AOC within the 0-10 cm layer was MBC, whereas within the 10-20 cm layer, EOC exhibited the highest sensitivity. EIM significantly improved soil carbon pool management indexes, with MBC being an important component factor of soil carbon pool management index. The results of redundancy analysis showed that the variation of soil organic carbon components was mainly affected by soil bulk density and pH. 【Conclusion】MBC serves as a sensitive indicator of changes in AOC components and a significant influencing factor in soil carbon pool management indexes. The comprehensive ecological farming mode enhances soil AOC components mainly by affecting bulk density and microbial activities, significantly elevating soil carbon storage. In conclusion, comprehensive ecological farming mode is an effective practice for carbon sequestration in paddy field. [ABSTRACT FROM AUTHOR]