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Copyright of China Forest Products Industry is the property of China Forest Products Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Harbin University of Science & Technology is the property of Journal of Harbin University of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electric Power Automation Equipment / Dianli Zidonghua Shebei is the property of Electric Power Automation Equipment Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: The effect of Polygonum cuspidatum extract on Pancreatic cancer cell line Panc-1 was investigated by extracting Polygonum cuspidatum extracts into human pancreatic cancer cell proliferation and apoptosis phenotype. Methods: Different concentrations(0, 10, 50, 100, 150, 200 μg/m L) of Polygonum cuspidatum extract were prepared, and each concentration of Polygonum cuspidatum extract was added to the human pancreatic cancer Panc-1 cell line to be treated for 24 h. The proliferation activity of the cell line Panc-1 was detected by CCK-8(cell counting kit-8) method; the human pancreatic cancer cell line Panc-1 was treated with 100 μg/m L Polygonum cuspidatum extract for 24 h, and then flow cytometry was used( FCM) was used to detect the cell cycle and apoptosis distribution; 100 μg/m L Polygonum cuspidatum extract was used to treat human pancreatic cancer cell line Panc-1 for 24 h, and the total RNA and total protein were extracted. Subsequent real-time PCR and Western blot were used to detect human pancreatic cancer.Transcription and translation levels of the cell line Panc-1 proliferation marker genes PCNA, CDK2 and apoptosis marker genes BAD,BAX. Results: The results of CCK-8 showed that the inhibition rate of Polygonum cuspidatum extract on the proliferation of human pancreatic cancer cell line Panc-1 increased with concentration. The results of flow cytometry showed that Polygonum cuspidatum extract inhibited the proliferation of human pancreatic cancer cells and promoted its apoptosis. The results of real-time PCR and Western blot showed The extract of Polygonum cuspidatum can decrease the expression of PCNA and CDK2 in human pancreatic cancer cell proliferation, and the expression of apoptosis marker gene BAD and BAX increased. Conclusion: Polygonum cuspidatum extract can inhibit the proliferation and promote apoptosis of human pancreatic cancer cell line Panc-1 [ABSTRACT FROM AUTHOR]

Ｏｂｊｅｃｔｉｖｅ　Ｔｏ ｃｏｎｓｔｒｕｃｔ ａ ｐｒｏｔｅｉｎ ｉｎｔｅｒａｃｔｉｏｎ ｎｅｔｗｏｒｋ ｂａｓｅｄ ｏｎ ｈｉｇｈ － ｔｈｒｏｕｇｈｐｕｔ ｓｅｑｕｅｎｃｉｎｇ ｄａｔａ ｏｆ ｌｉｖｅｒ ｃａｎｃｅｒ － ｒｅｌａｔｅｄ ｎｏｎ － ｃｏｄｉｎｇ ＲＮＡｓ，ｔｏ ｐｅｒｆｏｒｍ ａ ｆｕｎｃｔｉｏｎａｌ ｅｎｒｉｃｈｍｅｎｔ ａｎａｌｙｓｉｓ，ａｎｄ ｔｏ ｓｃｒｅｅｎ ｏｕｔ ｃｉｒｃｕｌａｒ ＲＮＡｓ （ｃｉｒｃＲＮＡｓ）ｐａｒｔｉｃｉｐａｔｉｎｇ ｉｎ ｔｈｅ ｄｅｖｅｌｏｐｍｅｎｔ ａｎｄ ｐｒｏｇｒｅｓｓｉｏｎ ｏｆ ｌｉｖｅｒ ｃａｎｃｅｒ ｖｉａ ｔｈｅ ｍｅｃｈａｎｉｓｍ ｏｆ ｃｏｍｐｅｔｉｔｉｖｅ ｅｎｄｏｇｅｎｏｕｓ ＲＮＡ （ｃｅＲＮＡ）． Ｍｅｔｈｏｄｓ　Ｔｈｅ ｃｉｒｃＲＮＡ － ｍｉＲＮＡ － ｍＲＮＡ ｎｅｔｗｏｒｋ ｗａｓ ｃｏｎｓｔｒｕｃｔｅｄ ｕｓｉｎｇ ｇｅｎｅ ｅｘｐｒｅｓｓｉｏｎ ｏｍｎｉｂｕｓ （ＧＥＯ）ｄａｔａ ｂａｓｅｄ ｏｎ ｔｈｅ ｃｅＲＮＡ ｔｈｅｏｒｙ． Ｇｅｎｅ ｏｎｔｏｌｏｇｙ （ＧＯ）ａｎｄ Ｋｙｏｔｏ Ｅｎｃｙｃｌｏｐｅｄｉａ ｏｆ Ｇｅｎｅｓ ａｎｄ Ｇｅｎｏｍｅ （ＫＥＧＧ）ａｎａｌｙｓｅｓ ｗｅｒｅ ｐｅｒｆｏｒｍｅｄ ｔｏ ｉｄｅｎｔｉｆｙ ｃｉｒｃＲＮＡｓ ｗｉｔｈ ｐｏｔｅｎｔｉａｌ ｃｅＲＮＡ ｆｕｎｃｔｉｏｎ ａｎｄ ｅｘｐｌｏｒｅ ｔｈｅｉｒ ｆｕｎｃｔｉｏｎｓ． Ｒｅｓｕｌｔｓ　Ａ ｔｏｔａｌ ｏｆ ９ ｃｏ － ｅｘｐｒｅｓｓｅｄ ｃｉｒｃＲＮＡｓ，２０ ｃｏ － ｅｘｐｒｅｓｓｅｄ ｍｉＲＮＡｓ，ａｎｄ １５３ ｃｏ － ｅｘｐｒｅｓｓｅｄ ｍＲＮＡｓ ｗｅｒｅ ｓｃｒｅｅｎｅｄ ｏｕｔ ｆｒｏｍ ｔｈｅ ＧＥＯ ｄａｔａｂａｓｅ，ａｎｄ ｔｈｅ ｌｉｖｅｒ ｃａｎｃｅｒ － ｒｅｌａｔｅｄ ｃｉｒｃＲＮＡ － ｍｉＲＮＡ － ｍＲＮＡ ｎｅｔｗｏｒｋ ｗａｓ ｓｕｃｃｅｓｓｆｕｌｌｙ ｃｏｎｓｔｒｕｃｔｅｄ． Ｔｈｅ ＧＯ ａｎａｌｙｓｉｓ ｒｅｖｅａｌｅｄ ９０ ｂｉｏｌｏｇｉｃａｌ ｐｒｏｃｅｓｓｅｓ，ｗｈｉｃｈ ｍａｉｎｌｙ ｉｎｖｏｌｖｅｄ １２ ｆｕｎｃｔｉｏｎａｌ ｃｌｕｓｔｅｒｓ ｉｎｃｌｕｄｉｎｇ ｈｅｐａｔｏｃｙｔｅ ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ，ｐｈａｓｅ － ｃｈａｎｇｅ ｒｅｇｕｌａｔｉｏｎ ｏｆ ｃｅｌｌ ｃｙｃｌｅ，ａｎｄ ｎｅｇａｔｉｖｅ ｒｅｇｕｌａｔｉｏｎ ｏｆ ｔｒａｎｓｃｒｉｐｔｉｏｎ ｆａｃｔｏｒ ａｃｔｉｖｉｔｙ． Ｔｈｅ ＫＥＧＧ ａｎａｌｙｓｉｓ ｓｈｏｗｅｄ ｔｈａｔ ｔｈｅ ｃｏ － ｅｘｐｒｅｓｓｅｄ ｃｉｒｃＲＮＡｓ ｗｅｒｅ ａｌｓｏ ｉｎｖｏｌｖｅｄ ｉｎ ｔｈｅ ｐ５３ ａｎｄ ＰＩ３Ｋ － Ａｋｔ ｓｉｇｎａｌｉｎｇ ｐａｔｈｗａｙｓ． Ｃｏｎｃｌｕｓｉｏｎ　Ｔｈｉｓ ｓｔｕｄｙ ｐｒｏｖｉｄｅｓ ｎｅｗ ｉｎｓｉｇｈｔｓ ｆｏｒ ｃｉｒｃＲＮＡｓ ｍｅｄｉａｔｉｎｇ ｔｈｅ ｄｅｖｅｌｏｐｍｅｎｔ ａｎｄ ｐｒｏｇｒｅｓｓｉｏｎ ｏｆ ｌｉｖｅｒ ｃａｎｃｅｒ ｔｈｒｏｕｇｈ ｔｈｅ ｍｅｃｈａｎｉｓｍ ｏｆ ｃｅＲＮＡ． [ABSTRACT FROM AUTHOR]

Copyright of Journal of Clinical Hepatology / Linchuang Gandanbing Zazhi is the property of Journal of Clinical Hepatology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao is the property of Chinese Society of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao is the property of Chinese Society of Veterinary Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electric Machines & Control / Dianji Yu Kongzhi Xuebao is the property of Electric Machines & Control and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)