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Using potato variety ‘Xuanshu 2' as materials，we studied the changes of relative conductivity，content of chlorophyll，POD activity ，SOD activity，contents of MDA ，free pro line，water soluble sugar and water soluble protein under different low temperature stress by artificial，at the seedling stage of potatoes. Based on these physiological and biochemical indexes correlation analysis，Some key indexes were screened out which could evaluate the cold tolerance of potato seedlings，the results indicated that with the lowering of stress temperature and the prolonging of stress time，the relative conductivity of potato seedlings was increased，content of chlorophyll was decreased，POD activity and SOD activity were firstly increased and then decreased，increased slowly at last，content of MDA，free proline and water soluble sugar were firstly increased and then decreased， content of water soluble protein was constantly increased. The relative conductivity， content of chlorophyll，POD activity，SOD activity，water soluble sugar and water soluble protein，were significant or extremely significant respectively， but a single index could not evaluate the cold tolerance of potato seedlings. It must be combined with other indexes to a comprehensive evaluation. Evaluation on cold tolerance of 14 potato varieties were made through fuzzy comprehensive evaluation method，the cold tolerance order was Zhuanxinwu > Hui-2 > Jizhangshu12 > Lishu6 > Zhongshu18 > Jinshu16 > Mira > Favorita > Diantongshu1 > Dianshu716 > Mangguoyangyu > Xuanshu2 > Hezuo88 > Weiyu3. [ABSTRACT FROM AUTHOR]