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Copyright of Chinese Journal of Osteoporosis is the property of Chinese Journal of Osteoporosis and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effect and mechanism of local administration of oleanic acid on bone fracture healing in ovariectomized mice. Methods A total of 45 female C57BL/6 mice aged 3 months were randomly divided into sham group, model group and oleanic acid (OA) group, with 15 mice in each group. In the model group and OA group, osteoporosis model in mice was established by bilateral ovariectomy (OVX), while only parovarian adipose tissue was removed in the sham mice. Three months after OVX, a middle fracture model of the left tibia was established, and an implantable osmotic pump was implanted simultaneously during the operation, with local sustained-release of OA in OA group. At 14 and 28 days after the operation, the morphology of callus tissue, bone trabecular microstructure, pathological changes, and the expression levels of Notch signaling pathway target gene and osteogenic related genes were observed. Results The three-dimensional reconstruction of left tibia showed that the fracture lines of the sham group and OA group were fuzzy at 14 days after fracture, and a large number of callus were formed. The fracture line of model group was still clear. At 28 days after fracture, the callus of the sham group and OA group had almost finished remodeling, while there was still larger callus in the model group. The mRNA expression level of Notch signaling pathway target gene Hes1 and RUNt-related transcription factor 2 (Runx 2), osteocalcin (OCN) and alkaline phosphatase (ALP) in callus of the model group were increased by 14 days after fracture compared with those of sham operation group (P<0.05). Compared with the model group, the expression level of Hes1 mRNA in callus of the OA group was decreased (P<0.05), while the expression levels of Runx 2 and OCN mRNA were increased (P<0.05). At 28 days after fracture, there was no significant difference in the mRNA expression levels of Hes1 among different groups, and the mRNA expression levels of Runx2, ALP and OCN in the OA group and the model group were lower than those in the sham operation group (P<0.05), but there was no significant difference in the mRNA expression levels of Runx 2 and ALP between the OA group and the model group, while the mRNA expression level of OCN in the OA group was higher than that in the model group (P<0.05). At 14 days after fracture, gait analysis results showed that the average walking time of left posterior limb in OA group was less than that in model group (P<0.05). Conclusion Local sustained-release OA down-regulated the expression of the target gene of Notch signaling pathway, and up-regulate the expressions of osteogenic related genes, so as to promote the fracture healing of ovariectomized mice. [ABSTRACT FROM AUTHOR]

To observe the effect of Huayu Bushen Formula on fracture healing in ovariectomized mice. Methods 36 female C57BL / 6 mice of 3 month old were randomly divided into sham group, model group and Huayu Bushen Formula group with 12 mice in each group. In the model group and Huayu Bushen formula group, the osteoporosis model was established by ovariectomy (OVX). Twelve weeks after OVX, the left middle tibial fracture model was established in all the mice. After operation, mice in Huayu Bushen Formula group were given Huayu Bushen formula by intragastric administration, and mice in sham group and model group were given equal volume of normal saline. The serum estradiol content was measured 4 weeks after fracture operation. The bone mineral density (BMD) of the fourth lumbar (L4) vertebrae and the left tibias were measured by Micro-?CT, and the moment of inertia of the left tibia was calculated. The paraffin sections of the left tibias were stained with Alcian blue / Orange G to observe the morphological changes of the callus. Results From 4 weeks after OVX, the body weight of the model group increased significantly, and continued to be higher than that of the sham operation group (P<0. 01). The serum estradiol level and L4 BMD of model group were significantly lower than that of sham group (P<0. 01), and were increased in Huayu Bushen Recipe group compared with those of model group (P <0?, 01). The 3 D reconstruction images of left tibias showed that obvious fracture line in the model group but not in sham group and Huayu Bushen Formula group. Micro-CT analysis also showed that compared with sham group, the BV / TV (P <0. 01), Tb. Th (P <0. 05) and Tb. N (P <0. 01) of callus of model group were significantly lower, and Tb.Sp was increased (P<0. 01), while BMD (P<0. 01), BV / TV (P<0. 05) and Tb.Th (P<0. 05) of the Huayu Bushen Formula group were significantly higher than those of the model group. There was no significant difference in Tb.N and Tb.Sp between the two groups. Compared with the sham group, the moment of inertia of callus in the model group was significantly higher (P<0. 01), while it was significantly decreased in the Huayu Bushen Formula group compared with that of the sham operation group (P<0. 05). Alcian blue / Orange G staining showed that in the sham group and Huayu Bushen Formula group, the callus tissue was in the shaping stage with trabecular bones in it, while there was plenty of adipose tissue in the callus of the model group with very few trabecular bone formation. Conclusion Huayu Bushen Formula can effectively promote fracture healing in ovariectomized mice, and improve the biomechanical properties of bone callus. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Osteoporosis is the property of Chinese Journal of Osteoporosis and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective By examining the effect of Gushukang granule (GSK) on weight, serum, and histological morphology of ovariectomized (OVX) mice, the pharmacological effect of GSK on delaying bone loss in OVX mice was further elaborated. Methods After OVX, GSK granules were administrated intragastrically to 3-month-old C57BL/6 J mice for 3 months, with low dose (2 g/kg·d), middle dose (4 g/kg·d), or high dose (8 g/kg·d) of GSK. Body weight was measured at 1-, 2-, and 3-month, respectively. At the end of the intervention, the expression of precollagen amino terminal peptide (PINPX), alkaline phosphatase (ALP), OCN, and β-CTX and were measured. At the same time, the changes of bone mass of the lumbar vertebrae were observed with Alcian blue staining. Results GSK granules exerted no significant effect on the body weight at the 1-, 2-, and 3- month of intervention. After 3 months of GSK intervention, the expression of β-CTX decreased, but the levels of bone formation markers PINP, ALP, and OCN increased. After GSK intervention, bone mass of the lumbar spine increased in OVX mice, and the bone structure-related parameters were optimized in the lumbar vertebrae. Conclusion GSK regulates the expression of bone resorption and bone formation parameters in OVX mice, delays the bone loss induced by OVX, and increases the bone mass in OVX mice. [ABSTRACT FROM AUTHOR]