Objective: To investigate the effects of long-chain non-coding RNA (LncRNA) MYU on the cycle distribution, cell proliferation, migration, invasion, and apoptosis of glioma cells, and to preliminarily explore its mechanism of action. Methods: The expression of LncRNA MYU in human brain normal glial cells HEB and glioma cells (U-251MG, A172, SHG139) was detected by real- time fluorescent quantitative PCR (RT-qPCR). SHG139 cells were selected and divided into normal control (NC) group, si-con group, and si-LncRNA MYU group for transfection experiments, and RT-qPCR was applied to detect the transfection effect. The effects of silencing LncRNA MYU on the cell cycle distribution and apoptosis, cell proliferation and, cell migration and invasion of SHG139 cells were detected respectively by flow cytometry, cell counting kit (CCK-8), and Transwell experiments. Western blot was used to detect the expression of matrix metalloproteinase (MMP-2), MMP-9, Cleaved caspase-3, Cleaved caspase-9, and Phosphatidylinositol-3-hydroxykinase/protein kinase B (PI3K/Akt) signaling pathway-related proteins. Results: The expression of LncRNA MYU in glioma cell lines was significantly higher than that in normal human brain glial cells(P<0.05), thereby SHG139 cells with the highest expression levels were selected for transfection experiments. Silencing LncRNA MYU can significantly induce G0-G1 phase arrest, inhibit the proliferation, migration and invasion, and induce apoptosis in SHG139 cells(P<0.05). Silencing LncRNA MYU can significantly inhibit the expression of MMP-2, MMP-9, p-PI3K and p-AKT, and promote the expression of Cleaved caspase-3 and Cleaved caspase-9(P<0.05). Conclusion: Silencing LncRNA MYU can induce G0-G1 phase arrest, inhibit cell proliferation, migration and invasion, and promote cell apoptosis in glioma cells, and its mechanism may be related to the inhibition of PI3K/AKT signaling pathway. [ABSTRACT FROM AUTHOR]