Subspecies differentiation of Chinese white-toothed shrew (Crocidura rapax) is controversial. In order to clarify the molecular phylogeographic pattern of C. rapax and provide further molecular biological evidence for taxonomy, this study analyzed the Cyt b gene sequences of C. rapax (20 samples) collected from Guizhou Province in China together with Cyt b gene sequences of C. rapax (19 samples) downloaded from GenBank. The phylogenetic tree was divided into three main clades: C. r. rapax, C. vorax, and C. rapax kurodai. The C. r. rapax is divided into Guizhou, Yunnan, southeast Sichuan lineage and central and west Sichuan lineage. The results of genetic distance showed that the greatest genetic distance was between central and west Sichuan lineage of C. r. rapax and C. r. kurodai (0. 1181), followed by between Guizhou, Yunnan, southeast Sichuan lineage of C. r. rapax and C. r. kurodai (0. 1174). The genetic distance between two lineages of C. r. rapax is 0. 0195. A similar geographical patterns was also observed in the median-joining network analysis. The results of median-joining network are consistent with the results of phylogenetic relationships and genetic distance, supporting the view that the sample from Taiwan is a separate species, namely, C. tadae. The C. rapax have no subspecies differentiation in China, and can be divided into Guizhou, Yunnan, southeast Sichuan lineage and central and west Sichuan lineage. Twenty-three haplotypes were found in the 32 samples of C. rapax, and the nucleotide polymorphism was 0. 01285. The analysis of population history suggested that C. rapax has experienced population expansion recently, but it is not a single dramatic expansion. [ABSTRACT FROM AUTHOR]