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目的为了探讨lncRNA DANCR在肿瘤细胞中的作用机制，本研究拟构建一种新的MS2-RIP方法用于鉴定DANCR的相互作用分子。方法利用MS2噬菌体衣壳蛋白可与噬菌体复制酶编码基因5’端一段由19个碱基组成的RNA茎环结构序列，即MS2结合位点（MS2 Binding Site，MS2 BS）高效结合的特点，构建串联表达MS2 BS和DANCR的过量表达质粒，通过MS2蛋白与MS2 BS的亲和作用，使DANCR得到富集，并且同步捕获与DANCR相互作用的分子，用于进一步分析鉴定。结果成功构建串联表达MS2 BS和DANCR的过量表达质粒用于MS2-RIP实验，该实验可显著富集DANCR以及作为阳性对照的DANCR结合蛋白EZH2。结论基于MS2蛋白与MS2结合位点的高效结合特性，我们针对lncRNA DANCR建立了MS2-RIP方法。该方法可有效富集DANCR以及与DANCR结合的生物活性分子，为研究DANCR的作用机制提供一种新的技术手段。

Lung transplantation is an effective means of treating various end-stage lung diseases. However, compared with other solid organ transplants, the survival rate after lung transplantation is relatively low. The main reason is the numerous complications after lung transplantation, among which infection is one of the most common complications. Respiratory viral infections are an important type of infection after lung transplantation, which severely affect the survival time and quality of life of lung transplant recipients. Early identification, early prevention, and active diagnosis and treatment are of great significance in reducing the incidence and fatality of respiratory viral infections after lung transplantation. This article reviews the epidemiology, risk factors, prevention and treatment principles, and specific prevention and treatment progress of common viruses in respiratory viral infections after lung transplantation at home and abroad, in order to provide a reference for the prevention and treatment of respiratory viral infections after lung transplantation in clinical practice. [ABSTRACT FROM AUTHOR]

This experiment was conducted to investigate the effects of different fermentation methods on the fermentation quality and microbial diversity of brewer's grains. The experiment was used functional oligosaccharides and compound probiotics to ferment brewer's grains, and divided into 4 groups: Group A was the control group (unfermented group), Group B was the barreled fermentation group, Group C was the traditional compaction fermentation group, Group D was water-sealed fermentation group. The fermentation time was 14 days. The results showed that the concentrations of crude protein, soluble carbohydrate and neutral detergent fiber in brewer's grains in group B were significantly higher than those in Group A (P<0.05). The pH value and ammonia nitrogen content of Groups B, C and D were significantly lower than those of the Group A (P<0.05). The lactic acid and acetic acid concentrations of Groups B, C, and D were significantly higher than those of Group A (P<0.05). In addition, the content of lactic acid in Group B was significantly higher than that in Groups C and D (P<0.05), and the content of lactic acid in Group D was significantly higher than that in Group C (P<0.05). Butyric acid was not detected in any of the groups. The relative abundances of firmicutes in Groups B, C, and D were higher than that in Group A, but the relative abundances of proteobacteria were lower than that in Group A. The dominant species in Groups A and C were Bacillus, while the dominant species in Groups B and D were Lactobacillus. The study indicates that the quality of fermented brewer's grains is best when using the barrel fermentation method, followed by the hydraulic pressure capping method, and worse when using the traditional compacting method in silos. [ABSTRACT FROM AUTHOR]

纤维支气管镜（以下简称为纤支镜）广泛地用于肺部疾病的诊断;对于肺癌的早期诊断,更是日益受到重视。但由于纤支镜取材少,组织易受挤压变形而造成误诊。我们从80年10月～85年12月收检经纤支镜取材的支气管组织共911例次（小部分病人活检1次以上）,其中267例诊断为肺癌。本文就其病理类型、特征及引起误诊的原因作一分析。 材料和方法 所有病例均采用Olympus,BF—B2或BF—B3型纤支镜。按常规术前用药和麻醉,经鼻孔进镜。本组病例进镜取材根据临床记载仅限于段支气管开口,所以均属中央型。每例取组织2～4块,放入10％福尔马林液固定,常规脱水,石蜡包埋切片,HE染色;部分病例作爱先蓝（Alcian blue）和银纤维染色。我们复查了全部切片和临床资料,部分病例作了随访。

系统性红斑性狼疮(简称SLE)是一种自身免疫性疾病,多器官受到损害,临床症状和病理改变比较复杂。国内较大宗的尸解材料研究尚未见有报道。我教研室从1947年5月13日到1982年2月24日在8496例尸解中确诊为本病的有23例。全部病例均作系统尸解,组织用福尔马林液固定,石腊包埋制片,HE染色,部分切片曾作PAS和纤维素染色。我们复习尸解记录和玻片,归纳描述各器官的病理改变,以期反映我国自己的材料的特点,从而提高对本病的认识,特别对狼疮性肾炎的病理组织学分型及其临床预后的关系作一探讨。

Copyright of Computer Measurement & Control is the property of Magazine Agency of Computer Measurement & Control and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Feed Industry is the property of Liaoning Provincial Institute of Agricultural Mechanization and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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目的 探讨规范尿标本留取流程对尿培养标本合格率的影响。方法 前瞻性分析2018年4 月至11月该院330例诊断为泌尿系结石术前进行中段尿培养患者的临床资料,使用随机数字表法分为改良组 (168例)和对照组(162例)。改良组实施规范宣教、清洗、留取、存放、送检、质量控制6个环节流程,对照组进 行常规宣教。比较2组患者标本污染率、住院时间、术前住院时间等,探索尿培养标本留取污染的危险因素。 结果 2组患者年龄、性别、文化程度比较,差异均无统计学意义(P >0.05);改良组患者尿培养标本污染率、术 前住院时间、总住院时间均明显优于对照组,差异均有统计学意义(P <0.05);年龄大、女性患者、文化程度低为 尿培养标本污染的独立危险因素。结论 规范尿培养留取流程可明显降低泌尿系结石患者尿培养标本污染 率,有效缩短术前住院时间及总住院时间。 [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Bioprocess Engineering is the property of Chinese Journal of Bioprocess Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Traditional runtimes based on user estimating is usually less accurate. This paper combined the categorization with the instance-based learning method, used the template similarity and numerical similarity method to find the similar jobs of the current jobs in historical data, and used historical scheduling data to predict the runtimes of the current jobs. This paper only took seven job attributes into account, which included user name, group name, queue name, application name, requested number of processors, requested runtime, requested memory. It applied genetic algorithm to train the best parameters, and used similar jobs attributes to predict runtimes. Compared with the existing method, experimental results show that the proposed prediction method achieves a similar underestimate rate on the premise of using fewer parameters, and gets a lower mean absolute error. Moreover, on the HPC2N04 and HPC2N05 datasets, the mean absolute errors reduce 43% and 77% respectively. This paper studied the effect of using online prediction to replace user estimation on job scheduling, analyzed the results and pointed out the future improvement directions. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the effect of virtual reality ( VR) technology physical therapy on improving insomnia in stroke patients. Methods The convenient sampling method was adopted. Fifty patients with insomnia after stroke treated in the hospital from April 2017 to December 2018 were divided into the study group (V R group) and control group (drug group ) by using the random number table, 25 cases in each group. The VR group used the VR technique physical therapy, and t he drug group was orally given the short acting benzodiazepines. All cases were continuously observed for 2 weeks. The latency to non - rapid eye movement( NREM ), number of awakenings, scores o f the Hamilton Anxiety Scale ( HAMA) and the Simple Intelligence Status Examination Scale (MMSE) at admission and discharge were assessed in the two groups and statistically analyzed. The sleep time and sleep conditions were com pared between the two groups. The effect of VR technology physical therapy on imp roving insomnia in stroke patients and its influence on the quality of life and cognitive function were analyzed. Results The latency of sleep NREM at discharge in the VR group was significantly lower than that at admission, and the difference was statistically significant (P< 0.05). The latency of sleep NREM and the number of awakenings at admission and discharge had no statistical difference between the V R group and drug group ( P> 0.05) . The MMSE score and H AMA score at discharge had statistical difference between the V R group and drug group ( P< 0.05 ). Conclusion The VR technology physical therapy has significant effect for improving the insomnia in stroke patients, and has no adverse effects on their quality of life and cognitive function, which can serve as a reference scheme for clinical optimization treatment. [ABSTRACT FROM AUTHOR]

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Copyright of Computer Engineering & Science / Jisuanji Gongcheng yu Kexue is the property of Computer Engineering & Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Practical Pharmacy & Clinical Remedies is the property of Editorial Department of Practical Pharmacy & Clinical Remedies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)