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Maize（Zea mays L.）originated in the subtropical zone, is a thermophilic crop, and is susceptible to low temperature stress. Dehydrin, group 2 members of the late embryogenesis abundant protein（LEA）, are a class of proteins that play an important role in plant abiotic stress. In this study, the ZmDHN15 gene was cloned and obtained, and the basic characteristics and tissue expression characteristics of the gene were analyzed using bioinformatics methods, real-time fluorescent quantitative PCR and other techniques, and the construction of plant overexpression vector and the genetic transformation of tobacco were carried out. T2 generation plants were used to verify their cold resistance. The results showed that the ZmDHN15 gene was 1 442 bp in length, encoded a total of 290 amino acids, with a molecular weight of 31.44 kD and a theoretical isoelectric point of 6.05. It was a hydrophilic non-transmembrane protein with a conserved domain unique to the dehydratin family. RT-qPCR analysis showed that the ZmDHN15 gene was highly expressed in maize leaves and increased under cold stress conditions. Nine T2 generation transgenic tobacco plants were obtained. Compared with the wild type, the germination rate of transgenic tobacco increased by 1.40 times, the root length increased by 1.58 times, the wilting degree of its leaves was lower, the content of proline, malondialdehyde and peroxidase reduced by 41.17%, 28.47% and 23.33%, respectively. Soluble sugar content, oxide activity and superoxide dismutase activity increased by 58.97%, 47.85% and 47.53%, respectively, and the accumulation of H2O2 and O2 - decreased by 34.78% and 47.00%, respectively. In conclusion, overexpression of ZmDHN15 gene can effectively improve the tolerance of tobacco plants to cold stress, which lays a foundation for further research on the function of ZmDHN15 gene in maize. [ABSTRACT FROM AUTHOR]

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