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摘要：【目的】研究可能与LOC389023和二肽基肽酶10（DPP10）作用的microRNA 在难治性颞叶癫痫患者皮质中的 表达变化，预测LOC389023在颞叶癫痫中的分子海绵机制。【方法】15例脑外伤患者切除的皮质为正常组，26例难治性颞叶 癫痫患者行癫痫灶切除的皮质为癫痫组。运用western blot、免疫组织化学染色检测DPP10和电压依赖性钾通道4.3（Kv4.3） 的表达变化，采用免疫荧光染色对DPP10和kv4.3进行定位，采用免疫共沉淀分析DPP10与Kv4.3的相关性。运用miRanda、 Pita、TargetScan和miRDB软件分别预测与LOC389023以及DPP10相互作用的microRNA。采用qPCR技术检测LOC389023以 及microRNA 的表达变化。【结果】免疫组织化学染色和western blot 分析结果显示，DPP10 在癫痫组中高表达（P < 0.05）， Kv4.3 则低表达（P < 0.05）；DPP10 与Kv4.3 共表达于神经元的细胞膜且两者有相互作用；通过软件预测得到5 个候选 microRNA（miR-32-5p，miR-140-5p，miR-367-3p，miR-25-3p和miR-4325）；qPCR检测结果显示：在癫痫组中，LOC389023 和miR-140-5p表达水平均低于对照组（P＜0.05），miR-25-3p和miR-367-3p表达水平均高于对照组（P < 0.05），而miR-32-5p 和miR-4325无明显变化（P > 0.05）。【结论】LOC389023可能以分子海绵的机制调控miR-25-3p和miR-367-3p，继而调控 DPP10的表达参与癫痫的形成。

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A method for using the effective damage area to set up the test site is proposed to collect the enough effective damage data in the equipment battle damage test. A solving model for effective damage area is established,and a concept of generalized damage area and task-based equipment functional damage probability is proposed. For the solution of generalized damage area,the physical damage modeling and simulation methods are studied,including fragment damage effectiveness model,equipment description model for fragment damage simulation,equipment fragment damage response model and equipment damage simulation process model. For the solution of equipment functional damage probability,the establishment method and steps of equipment damage simulation model are analyzed. A concept of equipment damage injection is proposed,and the implementation methods and steps of damage injection are analyzed. The simulated results show that the proposed method for solving the effective damage area of equipment is feasible and effective,and has great practical significance to optimize the design of battle damage test program and reduce the cost-effectiveness of test. [ABSTRACT FROM AUTHOR]

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In wartime, the spare parts warehouse or transport carrier may be attacked deliberately, and some storage and transportation capacity may be completely or partially lost, which leads to the interruption of spare parts supply system. The interruption risk of supply network is an uncertain parameter and difficult to be predicted and change dynamically. In view of the depth uncertainty of interruption risk, the diversification of wartime spare parts supply optimization objectives is considered. A joint optimization model of multi-objective location-allocation for wartime spare parts supply is constructed for the minimum cost of spare parts supply and the shortest lead time of supply. A corresponding robust optimization model is established based on the robust equivalent change of polyhedron uncertainty set. The meta-heuristic algorithms are used to obtain the non-dominate solution sets and the corresponding supply plans. The results show that the efficiency and reliability of spare parts supply network can be greatly improved by strengthening some key nodes. On the other hand, the optimal solution of the robust optimization model can guarantee the feasibility of the worst-case solution of spare parts supply, that is, the model has good robustness. [ABSTRACT FROM AUTHOR]

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The application of rock-chiseling hammer in breaking rock and clearing reef is a convenient and environmental-friendly construction technique, which has such advantages as minimized destruction to the existing structures and facilities adjacent to the quay to be reconstructed, convenient, and easy operation maneuver. We elaborate on the application of rock-chiseling hammer in rock-breaking and reef-clearing during construction of foundation bed in explosive-forbidden project by expounding the construction theory, construction technique, quality control and the result of its application for the purpose of serving as a valuable reference for explosive-forbidden project such as reconstruction and extension projects. [ABSTRACT FROM AUTHOR]