Search

Objective To investigate the expression of glucose regulated protein 78 (GRP78) and its significance in the renal carcinoma tissues. Methods The immunohistochemistry (IHC) method was used to detect the expression of GRP78 in 108 cases of renal carcinoma tissues and para-carcinoma tissues. According to the expression and percentage, the expression of GRP78 was divided into the positive expression and negative expression. The relationships between the GRP78 expression and clinical pathologic features of patients were analyzed. The Kaplan-Meier was used to calculate the overall survival (OS) of different expression of GRP78, and the Log-rank test was applied to compare. We adopted the Cox regression model to analyze the influence factors of OS. Results The positive rate of GRP78 expression in the tumor tissue was 72.3% (78/108), 19.4% (21/108) in para-carcinoma tissues, and significant difference was found between them (P<0.05). The expression of GRP78 in the renal carcinoma was correlated with lymph node metastasis and clinical stage (all P<0.05), and was not related with sex, age, diameter of tumor, pathological type and Fuhrman stage (all P>0.05). The overall survival time of patients with positive and negative expression of GRP78 in renal carcinoma was (57.9 ±8.6) and (77.2 ±7.9) months, respectively. The overall survival time of patients with positive GRP78 expression was significantly lower than that of patients with negative GRP78 expression (P<0.05). Univariate analysis demonstrated GRP78 expression, clinical stage, grade of Fuhrman and lymph node metastasis were the influence factor for OS; Multivariate analysis confirmed that the positive expression of GRP78, grade of Fuhrman and lymph node metastasis were three independent prognostic factors for OS (all P<0.05). Conclusion GRP78 expression was increased in the renal carcinoma and its high expression was related with OS of patients. [ABSTRACT FROM AUTHOR] more...

Objective To invesigate the expression of long non-coding RNA (lncRNA) PTV1 and its significance in the renal carcinoma tissues. Methods Fifty-four cases of renal cell carcinoma tissues were collected and the adjacent tissues of above 2 cm to tumor (no cancer cells were found under microscope) were used as the controls. Total RNA was isolated to get cDNA by performing reverse transcription. Then real-time quantitative PCR (RT-qPCR) was used to detect the expression of PTV1 in the renal cell carcinoma tissues and the adjacent tissues. Then the PTV1 expression level in the renal carcinoma tissues and the relationships between the expression of PTV1 and clinical characteristics and the prognosis were analyzed. The cell proliferation and apoptosis rate were tested by using MTT and flow cytometry, respectively. Results The relative expression levels of PVT1 in the renal carcinoma tissues and adjacent tissues were 6.22 ±0.71 and 2.26 ±0. 36, respectively, and the expression in the renal cell carcinoma tissues was significantly higher than that of the adjacent carcinoma tissues (P<0.05). The high expression of PVT1 in the renal carcinoma tissues was found in 39 cases of 54 cases, and 15 cases were low expression. The relative expression of PTV1 in renal carcinoma tissues was related with lymph node metastasis and TNM stage (all P<0.05), and was not related with sex, age and pathological types (all P>0.05). The overall survival time of patients with high expression and low expression of PVT1 in the renal carcinoma tissues was 47. 9 ±9.4 and 67.2 ±3.8 months, the overall survival time of patients with high PTV1 expression was significantly lower than that of patients with low PTV1 expression (P<0.05). Moreover, knockdown PVT1 expression in 786-0 cell line decreased the proliferation activity and enhanced the apoptosis rate (all P<0.05). Conclusion PVT1 may promote the occurrence and development of renal carcinoma by affecting the proliferation and apoptosis of renal cells, which can be used as a potential new target for renal cell carcinoma. [ABSTRACT FROM AUTHOR] more...