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[Objective] The paper aimed to study the effect of overexpression of Panax notoginseng PASS gene on early blight resistance in potato. [Method] The PASS gene was cloned from the leaves of P. notoginseng and a phylogenetic tree of P. notoginseng PnSS and its homologs was constructed using neighbor-joining method. Potato Jianchuan hong' was transformed using Agrobacterium mediated method. Two transgenic lines were randomly selected and inoculated with the pathogen Alternaria alternata under natural conditions. The disease index was quantified, and the expression levels of the auxin synthesis gene StYUCCAS and the auxin efflux carrier encoding genes StPIN1 and StPIN2 were detected using PCR. [Result] The PASS gene was cloned from P. notoginseng, and the phylogenetic tree showed that the P. notoginseng PnSS and its P. ginseng homologous were in the same cluster, but was in a different cluster with its Solanaceae homologous. After the transformation of P. notoginseng PASS gene into potato, 12 transgenic lines were obtained. Two lines were randomly selected and inoculated with A. alternata. It was found that transgenic potato plants expressing PaSS showed resistance to early blight. Furthermore, the transgenic potatoes showed a decreased levels of StYUCCA8 and StPIN2 before and after the plants inoculated with A. alternata, and the expression level of SIPINI increased before transgenic plants were inoculated with A. alternata, while the expression level decreased after inoculation with A. alternata. [Conclusion] Overexpression of the PASS gene decreases the expression levels of auxin synthesis and auxin efflux carriers-related genes to promote the potato resistance to early blight, which establishes a theoretical basis for potato breeding to obtain potato line resistance to early blight using PaSS gene. [ABSTRACT FROM AUTHOR]

【Objective】Root system architecture plays an important role in plant development, and function of DEEPER ROOTING 1(DRO1) gene involves in regulating plant root growth angle.However, the biological information of potato DRO1 homologous gene remains unclear.【Method】In the present study, bioinformatics softwares were used to analyze the physical and chemical properties, subcellular localization, conserved domain, phylogenetic tree and protein structure of StDRO2 protein in potato.【Result】Results showed that the potato StDRO2 gene encoded a hydrophilic and unstable protein located in the nucleus and the amino acid sequence contained typical domain of IGT gene family.The protein secondary structure showed that StDRO2 protein had random curl, α-helix, β-turn and extended chain structure.Real-time quantitative PCR analysis showed that the expression level of StDRO2 in potato seedling leaves was significantly higher than that in roots and stems.Auxin treatment down regulated the expression level of StDRO2 gene in roots.It indicated that the expression of StDRO2 gene was negatively regulated by auxin.【Conclusion】StDRO2 was a member of IGT gene family and involved in regulating potato root system architecture.The bioinformatic and expression assay of potato StDRO2 protein provided us theoretical basis to analyze potato root development in future research. [ABSTRACT FROM AUTHOR]