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Objective: To explore the inhibitory effect of melatonin combined with MPA on the proliferation of endometrial hyperplasia cells and its mechanism. Methods: The well differentiated endometrial hyperplasia cell line Ishikawa and endometrial cancer cell line ECC1 were cultured under appropriate conditions. After treatment with melatonin and MPA alone or in combination for 48 hours, the proliferative activity of endometrial cell lines was detected. The Ishikawa cells treated with melatonin and MPA alone or in combination for 48 h were collected, the protein was extracted, and the expression of AKR1C1 was detected. Ishikawa cell line overexpressing AKR1C1 was used to detect the cell proliferation activity after melatonin and MPA were treated alone or in combination for 48 hours. Results: The inhibitory effect of melatonin combined with MPA on endometrial cancer cells and endometrial dysplastic cells was significantly higher than that of melatonin or MPA alone. Melatonin and MPA can inhibit the expression of AKR1C1, and the inhibition of AKR1C1 in combination is higher than that in combination. Conclusion: Melatonin can improve the sensitivity of endometrial cancer to MPA, reduce the dosage of MPA, inhibit the expression of AKR1C1 and reduce the metabolic rate of progesterone. The combination of melatonin and MPA brings new ideas to the treatment strategy of endometrial hyperplasia. [ABSTRACT FROM AUTHOR]

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