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Objective: To investigate the differential gene expression and function of peripheral blood monocytes in patients with coronary heart disease and acute myocardial infarction. Methods: Patients receiving coronary angiography in Kunming First People’s Hospital from September 2020 to September 2021 were collected and total RNA of peripheral blood monocytes was extracted. DNBSEQ platform was used for second-generation highthroughput sequencing. We tested and screened differentially expressed genes, and made KEGG and GO enrichment analysis. Results: There were 89 differentially expressed genes,67 up-regulated genes and 22 downregulated genes in acute myocardial infarction group compared with normal coronary artery group. Among them, there were 14 differential lncRNAs, 72 differential mRNAs, and 3 differential circRNAs. KEGG pathway enrichment analysis, KEGG disease enrichment analysis and KEGG molecular enrichment analysis were completed. GO cell composition enrichment analysis, GO cell function enrichment analysis and GO biological process molecular enrichment analysis were completed. Conclusions: 10 mRNAs,6 lncRNAs,and 2 circRNAs with significant differences were screened. The enrichment analysis involves infection,transcriptional disorder,PI3K Akt signaling pathway, lipid synthesis, phagocytic vesicle cavity, extracellular matrix, lipopolysaccharide binding, etc., which may be related to the occurrence and development of coronary atherosclerosis and the occurrence of acute thrombotic events. [ABSTRACT FROM AUTHOR]

Objective　To investigate the correlation between Pre β1-HDL and the predictive value of coronary artery disease severity in patients with coronary heart disease. Methods　In this study， 90 patients undergoing coronary intervention diagnosis and/or treatment in our hospital were enrolled sequentially. Among them， there were 70 patients with coronary artery disease （ including 15 patients with single coronary artery disease， 55 patients with multiple coronary artery disease） and 20 patients with normal coronary artery disease. Clinical baseline data of each group were collected. Blood preβ1-HDL concentration was determined by ELISA. SPSS was used for statistical analysis. Results　The results of this study showed that blood pre β1-HDL levels in patients with different severity of coronary artery disease had the statistically significant differences （P < 0.05）. Preβ1-HDL concentration was positively correlated with coronary Gensini score （ P < 0.000），and the correlation was （ r = 0.782）. Preβ1-HDL concentration was positively correlated with cTnT （P < 0.000），and the correlation was （r = 0.421）. The area under ROC curve （AUC） of blood Pre β1-HDL was 0.993，the diagnostic threshold was 3.66， and the sensitivity and specificity were 100% and 94.7%， respectively. Conclusion　Blood Pre β1-HDL concentration has been found to be a new indicator to predict the severity of coronary artery disease. [ABSTRACT FROM AUTHOR]

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Objective To investigate the correlation of the concentration of blood / urine beta-trace protein (βTP) in the prediction of contrast-induced nephropathy. Methods This study enrolled 88 patients with interventional diagnosis or treatment from september to December 2016 in the first hospital of Kunming and the patients were divided into two groups: CIN group (nn = 7)and non-CIN group (n = 81) . The concentration of blood / urine βTP was measured by ELISA method and the results was analysed with SPSS．Results There was significant difference in the level of blood βTP before the operation，2h after operation and 24h after the operation between the two groups(P<0.05)，so was that in the level of urine βTP(P<0.05) . The area under ROC curve (AUC) of blood βTP 2h after the operation was 0.761，the diagnostic value was 567.568，and the sensitivity and specificity were 71.4% and 81.5% while the area of ROC curve(AUC) of urine βTP 2h after the operation was 0.967，the diagnostic value was 1428.893，and the sensitivity and specificity were 100% and 97.5%．Conclusion The concentration of blood / urine βTP 2h after the operation can be used as a new indicator to predict contrast-induced nephropathy. The sensitivity and specificity of urine βTP is better than those of blood βTP，and urine samples are easier to be collected than blood samples. [ABSTRACT FROM AUTHOR]

Objective To investigate the correlation between blood / urine NGAL concentration and predictive value of contrast induced nephropathy. Methods A total of 88 patients who underwent coronary intervention and / or treatment were enrolled in our hospital from October to December in 2016. The patients with CIN were treated as CIN (7 cases) . In cases where CIN did not occur As non-CIN group (81 cases)， a total of 89 cases. The clinical baseline data were collected from each group. The concentration of NGAL in blood / urine was measured by ELISA. Statistical analysis was performed using SPSS20.0. Results The results showed that the difference of NGAL level between CIN group and non - CIN group was statistically significant (P<0.05) . There was significant difference in urine NGAL level between 2 h and 24 h after operation (P<0.05) . There was significant difference in urinary NGAL level between the CIN group and the non-CIN group (P<0.05) . In the present study， the area under the ROC curve (AUC) was 0.743， the diagnostic value of renal disease was 4.114， and the sensitivity and specificity were 85.7% and 64.2% respectively. In this study， ROC The area under the curve (AUC) was 0.961，and the sensitivity and specificity were 100%and 91.4%，respectively， when the diagnostic value of contrast medium nephropathy was 4.798. Conclusion Blood / urine NGAL 24h after coronary intervention can be used as a predictor of contrast nephropathy. [ABSTRACT FROM AUTHOR]

Objective To investigate the value of the plasma FKN concention in prediction of the severity of coronary heart disease（CHD） . Methods In this study, 89 patients who underwent coronary intervention therapy in our hospital between July and December 2015 were sequentially selected. Patients were divide into 3 groups according to the result of Coronary angiography，including group B: 43 patients of acute myocardial infarction （AMI） or Coronary Severe lesions；group B1: 26 patients of single mild Coronary lesions；group N: 20 patients with no Coronary heart disease（CHD），excluded by normal Coronary angiography result. The baseline clinical data of all 89 patients were collected. Enzyme-linked immunosorbent method was used to test the FKN level. SPSS 20.0 Statistical software was used for data analysis. Results The FKN levels of patients were decreased in all 3 groups: group B> group B1>group N，with significant differencesbetween the three groups（ ＜0.000） . There was a positive correlation between FKN and Gensini score or hs cTnT（p P＜0.05） . The area under the curve（AUC） of the receiver operating characteristic （ROC） curves was 0.784, the diagnostic reference value for Coronary heart disease（CHD） acute myocardial infarction was 1.996 in this sudy. Conclusions The plasma FKN levels are positively correlated to the severity of coronary lesion. The specificity and sensitivity of plasma FKN level for coronary heart disease （CHD） diagnosis is similar to CTnT. The plasma FKN levels can provide evidence of lesion severity of Coronary heart disease （CHD）. [ABSTRACT FROM AUTHOR]