This experiment was conducted to investigate the effects of Antrodia cinnamomea culture on performance, egg quality and serum antioxidant indexes of laying hens in late peak period. A total of 288 Hyline white laying hens (400 days of age) with similar body condition and laying performance in the late peak period of laying were randomly divided into four groups: control group and low, medium and high dose groups. Laying hens in control group were fed a basal diet, and laying hens in low, medium and high dose groups were fed the basal diet supplemented with 50 mg/kg, 100 mg/kg and 200 mg/kg Antrodia cinnamomea culture, respectively. The pre-test lasted for seven days and the experiment lasted for 28 days. The results showed as follows: compared with the control group, the laying rate, daily feed intake and daily egg weight in the Antrodia cinnamomea culture group were slightly increased, and the ratio of feed to egg in the 50 mg/kg and 100 mg/kg Antrodia cinnamomea culture groups were slightly lower than that in the control group, but the difference was not significant (P>0.05); Antrodia cinnamomea culture had no significant effect on egg quality. The proportion of dark red liver in 200 mg/kg Antrodia cinnamomea culture group was 83.33% with the increase of Antrodia cinnamomea culture dosage. With the increase of Antrodia cinnamomea culture, the pathological changes of liver tissue slices of laying hens could be reduced or disappeared; the superoxide dismutase (SOD) content of 200 mg/kg Antrodia cinnamomea culture group was significantly higher than that of 50 mg/kg group and control group (P<0.05), but the contents of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were not significantly different among all groups (P>0.05). When 50 g and 100 g Antrodia cinnamomea culture were added to the diet per ton, 72 laying hens fed for 28 days could gain an additional profit of 34.4 yuan and 58.4 yuan, respectively. In conclusion, Antrodia cinnamomea culture can improve the production performance of laying hens in the late peak period, reduce the incidence of fatty liver of laying hens, improve serum antioxidant indexes, and increase the breeding income of farmers, among which the 100 mg/kg group of Antrodia cinnamomea has the best profit. [ABSTRACT FROM AUTHOR]