Objective To identify lactobacilli that can inhibit Gardnerella vaginalis（ Gv） and Candida albicansor（ Ca） through isolation and screening from the vaginal secretion of healthy women and to evaluate their properties. Methods Vaginal swab samples were collected from 30 healthy women of childbearing age. The suspected lactobacillus strains were selected after several enriched culture-plate separation, and the strain type was determined by 16 S rDNA sequencing.The inhibitory effect of lactobacillus strains on the indicator bacteria（ 10 kinds of bacteria and 5 kinds of candida albicans）was evaluated by double layer agar diffusion assay, and the human vaginal lactobacillus with inhibitory Gv/Ca activity was screened out. The antimicrobial activity of the screened human vaginal lactobacillus was studied by coincubating Gv and Ca with cultures or supernatants of selected lactobacillus strains. Moreover, the hydrogen peroxide production capacity, the lactic acid production capacity, auto-aggregation and coaggregation and the biofilm formation ability of the lactobacillus strains screened out were observed. Results（1） Fifty-one strains were isolated from the vaginal secretion of 30 healthy women, and 23 strains had the characteristics of lactobacillus.16 S r DNA sequencing confirmed that all of them were lactobacillus.（2） Eight strains were selected based on their antagonist potential against Gv and/or Ca, including: strain 2 that only inhibited Gv, strains 7, 26 and 44 that only inhibited Ca, and strains 16, 19, 31, and 41 that inhibited both simultaneously. The above 8 strains were screened for the human vagina lactobacillus with inhibitory activity on Gv/Ca. Further, through co-cultivation of the strains and co-culture of the supernatants, strains 2, 7, 16, 31, and 41 all showed antibacterial activity against Gv, and strain 41 completely inhibited the growth of Gv. Similarly, strains 7, 16, 19, 26, 31, 41 and44 all had antibacterial activity against Ca; strain 19 supernatant completely inhibited the growth of Ca.（3） Statistical difference was found in the pH value, lactic acid generation and H2O2 generation of 8 lactobacillus strains as compared with those of negative controls（ all P < 0.05）. The strains 19 and 41 had the strongest acid production and H2O2 ability, and reached the level of positive control strains（ both P > 0.05）.（4） The auto-aggregation ability of Gv was 45.25%, while that of Ca was as high as 100%. Lactobacillus strains 16（ 92.57%）, 19（ 96.32%）, 31（ 92.16%） and 41（ 93.52%）showed significant auto-aggregation ability.（5） Strains 19, 26 and 41 showed relatively strong co-aggregation with Ca（ qualitative was + +, quantitative was 61.23%, 58.72% and 54.59%, respectively）, and the co-aggregation ability of other strains with Ca was 28.53%-44.21%. Strains 16, 19, and 41 showed relatively strong co-aggregation with Gv（ qualitative was + +, quantitative was 82.34%, 83.41% and 93.56, respectively）, and the co-aggregation ability of other strains with Gv was 41.33%-46.12%.（6）The 8 strains of lactobacillus were able to form biofilms, of which, strain 19 were strongest, followed by strain 41. Conclusions Eight strains of human vaginal lactobacillus with inhibitory activity on Gv/Ca are successfully screened out. All 8 strains of lactobacillus have antibacterial effect on Gv/Ca, and have strong ability to produce hydrogen peroxide, lactic acid, and biofilm formation. Among them, strains 19 and 41 have the potential to become new vaginal probiotics. [ABSTRACT FROM AUTHOR]