Objective To observe the effects of afzelin in the treatment of BALA/C mice with colorectal cancer and its influence on the anti-angiogenesis. Methods Sixty BALA/C mice were divided into the control group, cis-platinum group (20 mg/kg), high-dose afzelin group (100 mg/kg), medium-dose afzelin group (50 mg/kg) and low-dose afzelin group (25 mg/kg). Using LOVO colorectal cancer cells for axillary vaccination to build the colorectal cancer models. After the models were built, the afzelin was gavaged for thirty days. At 24 hours after the last gavage, mice in each group were sacrificed and the tumor tissues were taken, and the weight of tumor tissues, tumor inhibition rate and tumor volume were observed. The tumor tissues were divided into4 parts, the histopathologies were observed by HE staining in one part, while the vascular endothelial growth factor (VEGF), hvpoxia-inducible factor 1-α (HIF-lα) and vascular endothelial growth factor receptor (VEGFR-2) were detected by fluorescence immunoassay in other three parts in the five groups. Results Compared with the model group, the tumor volume and weight were significantly lower in the high-dose, medium-dose, and low-dose afzelin groups (both P <0.05). HE staining showed that the morphology and staining of cancer cells in the tumor tissues of the model group were normal, and the structure was intact. The tumor cells in the high-dose, medium-dose, and low-dose afzelin groups had large areas of apoptosis. Compared with the model group, the expression of VEGF, HIF-lα and VEGFR-2 decreased significantly in the high-dose, medium-dose, and low-dose afzelin groups (all P <0.05). Conclusion Afzelin in the treatment of BALA/C mice with colorectal cancer is effective, and has good anti-angiogenesis effects. [ABSTRACT FROM AUTHOR]